BGC0001845: alpiniamide biosynthetic gene cluster from Streptomyces sp. CBMAI 2042
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Location: 103,490 - 169,546 nt. (total: 66,057 nt).
This entry is originally from NCBI GenBank RCOL01000001.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
KnownClusterBlast
NRPS/PKS domains
General information about the BGC
MIBiG accession BGC0001845
Short description alpiniamide biosynthetic gene cluster from Streptomyces sp. CBMAI 2042
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Remarks "The gene cluster codifies alpiniamide (Nat Prod Commun. 2013 Oct;8(10):1393-6. (PMID 24354182), biosynthesized by a trans-AT-PKS-NRPS hybrid gene cluster. Other results were based on gene cluster prediction, gene knock out in the wild type strain and heterologous expression of the whole gene cluster."
Biosynthetic class(es)
  • other (aminocoumarin)
  • PKS (Unknown)
  • NRPS (Type I)
Loci
RCOL01000001.1
122924 - 152602
via Knock-out studies, Heterologous expression
Compounds
  • alpiniamide
Species Streptomyces sp. CBMAI 2042 [taxonomy]
References
Chemical products information
alpiniamide Evidence: NMR, Mass spectrometry
Copy SMILES
C17H29N1O6
Chemical database entries
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • STAN_0115
  • RLV64595.1
 122547 - 122963 (-) glyoxalase/bleomycin resistance protein/dioxygenase
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    • STAN_0116
    • RLV64596.1
    		 122974 - 123393 (-) transcriptional regulator, AraC family, isolated domain protein
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      • STAN_0117
      • RLV64597.1
      		 123490 - 124734 (-) Malonyl CoA-acyl carrier protein transacylase
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        • STAN_0118
        • RLV64598.1
        		 124779 - 125843 (-) 3-oxoacyl-ACP synthase III
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          • STAN_0119
          • RLV64599.1
          		 125840 - 134248 (-) Erythronolide synthase, modules 1 and 2
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            • STAN_0120
            • RLV64600.1
            		 134241 - 138713 (-) 6-deoxyerythronolide-B synthase
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              • STAN_0121
              • RLV64601.1
              		 138738 - 149546 (-) polyketide synthase of type I
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                • STAN_0122
                • RLV64602.1
                		 149813 - 150991 (-) Acyl-CoA dehydrogenase
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                  • STAN_0123
                  • RLV64603.1
                  		 151094 - 152239 (-) ribosomal RNA small subunit methyltransferase
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                    • STAN_0124
                    • RLV64604.1
                    		 152375 - 152554 (-) hypothetical protein
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                      Biosynthesis information

                      Biosynthetic modules

                      Name
                      2
                      Type
                      pks-trans-at
                      Genes
                      STAN_0121
                      Substrates
                      Integrated Monomers
                      Domains
                      ketosynthase, methyltransferase, carrier (ACP)
                      Name
                      3
                      Type
                      pks-trans-at
                      Genes
                      STAN_0121
                      Substrates
                      Integrated Monomers
                      Domains
                      ketosynthase, dehydratase, ketoreductase
                      Name
                      4
                      Type
                      pks-trans-at
                      Genes
                      STAN_0120
                      Substrates
                      Integrated Monomers
                      Domains
                      ketosynthase, thioesterase, methyltransferase, carrier (ACP), carrier (ACP)
                      Name
                      5
                      Type
                      pks-modular
                      Genes
                      STAN_0119
                      Substrates
                      Integrated Monomers
                      Domains
                      acyltransferase, ketosynthase, acyltransferase, carrier (ACP)
                      Name
                      6
                      Type
                      pks-trans-at
                      Genes
                      STAN_0119
                      Substrates
                      Integrated Monomers
                      Domains
                      ketosynthase, dehydratase, ketoreductase, carrier (ACP)
                      Name
                      1
                      Type
                      nrps-type1
                      Genes
                      STAN_0121
                      Substrates
                      glycine (evidence: Sequence-based prediction)
                      Integrated Monomers
                      Domains
                      condensation (LCL), adenylation
                      Annotation changelog

                      Entry version: 1

                      Date
                      Changes
                      Submitters
                      Reviewers
                      2022-09-15
                      Submitted
                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                      2022-09-15
                      Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                      2022-09-15
                      Removed empty PKS module modifiers
                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                      2022-09-15
                      Fetch compound properties from PubChem
                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                      2022-09-15
                      Update comment
                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                      2022-09-15
                      Changed amino acid substrates to lower case
                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                      2022-09-15
                      Released from embargo
                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
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                      Detailed domain annotation
                      Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
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