BGC0001853: Largimycin A1 biosynthetic gene cluster from Streptomyces argillaceus
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Location: 1 - 69,807 nt. (total: 69,807 nt).
This entry is originally from NCBI GenBank LR131959.1, but has been modified (see Modifications tab for details).

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
Modifications
General information about the BGC
MIBiG accession BGC0001853
Short description Largimycin A1 biosynthetic gene cluster from Streptomyces argillaceus
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Remarks "Largimycin biosynthesis gene cluster"
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Type I)
Loci
LR131959.1
1 - 69807
via Knock-out studies
Compounds
  • Largimycin A1
  • Largimycin A2
  • Largimycin A3
Species Streptomyces argillaceus [taxonomy]
References
Chemical products information
Largimycin A1 Evidence: NMR [1]
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C33H40N4O14S3
Chemical database entries
NPAtlas
Largimycin A2 Evidence: NMR [1]
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C28H31N3O11S2
Chemical database entries
NPAtlas
Largimycin A3 Evidence: Mass spectrometry [1]
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C23H23Cl1N2O8S1
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • ctg3_1
 1 - 1530 (-)
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    • ctg3_2
    		 1687 - 2304 (+)
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      • ctg3_3
      		 2506 - 2928 (-)
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        • ctg3_4
        		 3553 - 4911 (+)
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          • ctg3_5
          		 5671 - 6339 (+)
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            • ctg3_6
            		 6321 - 7073 (-)
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              • ctg3_7
              		 7084 - 8385 (-)
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                • ctg3_8
                		 8715 - 9473 (+)
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                  • ctg3_9
                  		 9544 - 10857 (+)
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                    • ctg3_10
                    		 10928 - 11845 (+)
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                      • ctg3_11
                      		 11829 - 13061 (+)
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                        • ctg3_12
                        		 13066 - 13857 (+)
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                          • ctg3_13
                          		 13854 - 16250 (+)
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                            • ctg3_14
                            		 16353 - 17303 (+)
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                              • ctg3_15
                              		 17365 - 19164 (+)
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                                • ctg3_16
                                		 19185 - 20129 (+)
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                                  • ctg3_17
                                  		 20165 - 21340 (+)
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                                    • ctg3_18
                                    		 21353 - 34219 (+)
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                                      • ctg3_19
                                      		 34216 - 56238 (+)
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                                        • ctg3_20
                                        		 56238 - 57227 (+)
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                                          • ctg3_21
                                          		 57211 - 57474 (+)
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                                            • ctg3_22
                                            		 57471 - 58709 (+)
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                                              • ctg3_23
                                              		 58722 - 58964 (+)
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                                                • ctg3_24
                                                		 58961 - 60232 (+)
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                                                  • ctg3_25
                                                  		 60234 - 60995 (+)
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                                                    • ctg3_26
                                                    		 60992 - 61711 (+)
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                                                      • ctg3_27
                                                      		 61762 - 62160 (-)
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                                                        • ctg3_28
                                                        		 62314 - 63777 (+)
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                                                          • ctg3_29
                                                          		 63908 - 64240 (+)
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                                                            • ctg3_30
                                                            		 64230 - 64835 (+)
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                                                              • ctg3_31
                                                              		 64961 - 66148 (+)
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                                                                • ctg3_32
                                                                		 66321 - 66413 (-)
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                                                                  • ctg3_33
                                                                  		 66507 - 66872 (+)
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                                                                    • ctg3_34
                                                                    		 67043 - 68527 (+)
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                                                                      • ctg3_35
                                                                      		 68563 - 69807 (+)
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                                                                        Biosynthesis information

                                                                        Biosynthetic modules

                                                                        Name
                                                                        1
                                                                        Type
                                                                        nrps-type1
                                                                        Genes
                                                                        ctg3_15
                                                                        Substrates
                                                                        threonine (evidence: Structure-based inference)
                                                                        Integrated Monomers
                                                                        Domains
                                                                        adenylation
                                                                        Name
                                                                        2
                                                                        Type
                                                                        nrps-type1
                                                                        Genes
                                                                        ctg3_18
                                                                        Substrates
                                                                        serine (evidence: Structure-based inference)
                                                                        Integrated Monomers
                                                                        Domains
                                                                        condensation (Heterocyclization), adenylation, oxidase
                                                                        Name
                                                                        3
                                                                        Type
                                                                        pks-trans-at
                                                                        Genes
                                                                        ctg3_18
                                                                        Substrates
                                                                        Integrated Monomers
                                                                        Domains
                                                                        ketosynthase, ketoreductase, carrier (ACP)
                                                                        Name
                                                                        3A
                                                                        Type
                                                                        pks-trans-at
                                                                        Genes
                                                                        ctg3_18
                                                                        Substrates
                                                                        Integrated Monomers
                                                                        Domains
                                                                        ketosynthase, carrier (ACP)
                                                                        Name
                                                                        4A
                                                                        Type
                                                                        pks-trans-at
                                                                        Genes
                                                                        ctg3_18
                                                                        Substrates
                                                                        Integrated Monomers
                                                                        Domains
                                                                        ketosynthase
                                                                        Name
                                                                        4B
                                                                        Type
                                                                        pks-trans-at-starter
                                                                        Genes
                                                                        ctg3_19
                                                                        Substrates
                                                                        Integrated Monomers
                                                                        Domains
                                                                        dehydratase, ketoreductase, carrier (ACP)
                                                                        Name
                                                                        5
                                                                        Type
                                                                        pks-trans-at
                                                                        Genes
                                                                        ctg3_19
                                                                        Substrates
                                                                        Integrated Monomers
                                                                        Domains
                                                                        ketosynthase, other (Enoyl-CoA dehydratase), other (Enoyl-CoA dehydratase), carrier (ACP)
                                                                        Name
                                                                        6
                                                                        Type
                                                                        pks-trans-at
                                                                        Genes
                                                                        ctg3_19
                                                                        Substrates
                                                                        Integrated Monomers
                                                                        Domains
                                                                        ketosynthase, dehydratase, ketoreductase, methyltransferase, carrier (ACP)
                                                                        Name
                                                                        7
                                                                        Type
                                                                        pks-trans-at
                                                                        Genes
                                                                        ctg3_19
                                                                        Substrates
                                                                        Integrated Monomers
                                                                        Domains
                                                                        ketosynthase, ketoreductase, carrier (ACP)
                                                                        Name
                                                                        8
                                                                        Type
                                                                        pks-trans-at
                                                                        Genes
                                                                        ctg3_19
                                                                        Substrates
                                                                        Integrated Monomers
                                                                        Domains
                                                                        ketosynthase, thioesterase, carrier (ACP), carrier (ACP)
                                                                        Annotation changelog

                                                                        Entry version: next

                                                                        Date
                                                                        Changes
                                                                        Submitters
                                                                        Reviewers
                                                                        2024-04-23
                                                                        MIBiG v4 annotathon
                                                                        • (ID: AA7SE7MC65QS2IJNSQXWZD7I)
                                                                        • (ID: YO3TP6VWB5CBNPZKNYNQSKUW)
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                                        Entry version: 2

                                                                        Date
                                                                        Changes
                                                                        Submitters
                                                                        Reviewers
                                                                        2022-09-15
                                                                        Fixed NRPS module numbering
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        2022-09-15
                                                                        Re-added to MIBiG
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        2022-09-15
                                                                        Corrected NRP module activity
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        2022-09-15
                                                                        Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        2022-09-15
                                                                        Reworked NRPS/PKS module information
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        2022-09-15
                                                                        Sorted modules by module number
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        2022-09-15
                                                                        Removed empty PKS module modifiers
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        2022-09-15
                                                                        Added citation
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        2022-09-15
                                                                        Removed empty optional properties
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        2022-09-15
                                                                        Changed amino acid substrates to lower case
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                                        Entry version: 1

                                                                        Date
                                                                        Changes
                                                                        Submitters
                                                                        Reviewers
                                                                        2019-10-16
                                                                        Submitted
                                                                        • (ID: 5GAWSZNTLIUCV6N473S7S3QZ)
                                                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                        Detailed domain annotation
                                                                        Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
                                                                        A domain glossary is available here, and an explanation of the visualisation is available here.
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                                                                        Modifications to original record
                                                                        • ctg3_1 was added
                                                                        • ctg3_2 was added
                                                                        • ctg3_3 was added
                                                                        • ctg3_4 was added
                                                                        • ctg3_5 was added
                                                                        • ctg3_6 was added
                                                                        • ctg3_7 was added
                                                                        • ctg3_8 was added
                                                                        • ctg3_9 was added
                                                                        • ctg3_10 was added
                                                                        • ctg3_11 was added
                                                                        • ctg3_12 was added
                                                                        • ctg3_13 was added
                                                                        • ctg3_14 was added
                                                                        • ctg3_15 was added
                                                                        • ctg3_16 was added
                                                                        • ctg3_17 was added
                                                                        • ctg3_18 was added
                                                                        • ctg3_19 was added
                                                                        • ctg3_20 was added
                                                                        • ctg3_21 was added
                                                                        • ctg3_22 was added
                                                                        • ctg3_23 was added
                                                                        • ctg3_24 was added
                                                                        • ctg3_25 was added
                                                                        • ctg3_26 was added
                                                                        • ctg3_27 was added
                                                                        • ctg3_28 was added
                                                                        • ctg3_29 was added
                                                                        • ctg3_30 was added
                                                                        • ctg3_31 was added
                                                                        • ctg3_32 was added
                                                                        • ctg3_33 was added
                                                                        • ctg3_34 was added
                                                                        • ctg3_35 was added