BGC0002124: SF2487 biosynthetic gene cluster from Actinomadura verrucosospora NRRL:B18236
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Location: 2,037,887 - 2,164,555 nt. (total: 126,669 nt).
This entry is originally from NCBI GenBank CP053892.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0002124
Short description SF2487 biosynthetic gene cluster from Actinomadura verrucosospora NRRL:B18236
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Remarks "Tetromadurin is a polyether tetronate polyketide. Chain release occurs concomitantly with tetronate ring formation. The KR of Module 12 appears inactive based on its sequence. However, the corresponding position on the acyl chain contains a D-OH group. The domain/responsible for this reduction is unclear."
Biosynthetic class(es)
  • PKS (Type I)
Loci
CP053892.1
2037887 - 2164555
via Knock-out studies
Compounds
  • SF2487
Species Actinomadura verrucosospora NRRL:B18236 [taxonomy]
References
Chemical products information
SF2487 Evidence: NMR, Mass spectrometry, X-ray crystallography, Chemical derivatisation
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Chemical database entries
PubCHEM
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • ACTIVE_1764
  • QKG20128.1
 2038117 - 2039118 (-) regulatory protein
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    • ACTIVE_1765
    • QKG20129.1
    		 2039157 - 2040320 (-) membrane transporter
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      • ACTIVE_1766
      • QKG20130.1
      		 2040401 - 2042209 (-) peptidase S9, prolyl oligopeptidase active site region
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        • ACTIVE_1767
        • QKG20131.1
        		 2042232 - 2043164 (-) transcriptional regulator TrmB
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          • ACTIVE_1768
          • QKG20132.1
          		 2043605 - 2044111 (+) response regulator receiver/SARP domain-containing protein
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            • ACTIVE_1769
            • QKG20133.1
            		 2044148 - 2044618 (+) hypothetical protein
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              • ACTIVE_1770
              • QKG20134.1
              		 2045152 - 2045403 (+) transposase for IS112
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                • ACTIVE_1771
                • QKG20135.1
                		 2045473 - 2045553 (-) hypothetical protein
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                  • ACTIVE_1772
                  • QKG20136.1
                  • madAII
                  		 2046104 - 2058040 (-) type I polyketide synthase
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                    • ACTIVE_1773
                    • QKG20137.1
                    • mad31
                    		 2058085 - 2058645 (-) tetrahydropyran 4+2 cyclase
                    • Mutation phenotype: <mibig.converters.shared.mibig.gene.function.MutationPhenotype object at 0x7fabd7adcf50>
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                    • ACTIVE_1774
                    • QKG20138.1
                    • mad30
                    		 2058862 - 2060064 (+) cytochrome P450
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                      • ACTIVE_1775
                      • QKG20139.1
                      • mad29
                      		 2060109 - 2061269 (-) cytochrome P450
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                        • ACTIVE_1776
                        • QKG20140.1
                        • madB
                        		 2061341 - 2061739 (-) epoxide hydrolase
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                          • ACTIVE_1777
                          • QKG20141.1
                          • mad27
                          		 2062041 - 2062583 (-) MarR family transcriptional regulator
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                            • ACTIVE_1778
                            • QKG20142.1
                            • mad26
                            		 2062645 - 2063583 (+) ABC transporter ATP-binding protein
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                              • ACTIVE_1779
                              • QKG20143.1
                              • mad25
                              		 2063580 - 2065187 (+) ABC transporter permease
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                                • ACTIVE_1780
                                • QKG20144.1
                                • madAIII
                                		 2065493 - 2070952 (+) type I polyketide synthase
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                                  • ACTIVE_1781
                                  • QKG20145.1
                                  • madAIV
                                  		 2070998 - 2081029 (+) type I polyketide synthase
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                                    • ACTIVE_1783
                                    • QKG20146.1
                                    • madAV
                                    		 2081071 - 2098083 (+) Type I polyketide synthase
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                                      • ACTIVE_1784
                                      • QKG20147.1
                                      • madAVI
                                      		 2098226 - 2115097 (+) type I polyketide synthase
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                                        • ACTIVE_1785
                                        • QKG20148.1
                                        • mad20
                                        		 2115851 - 2116672 (+) Hypothetical protein
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                                          • ACTIVE_1786
                                          • QKG20149.1
                                          • madC
                                          		 2116912 - 2118330 (-) Epoxidase
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                                            • ACTIVE_1787
                                            • QKG20150.1
                                            • mad18
                                            		 2118378 - 2119529 (-) dehydratase
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                                              • ACTIVE_1788
                                              • QKG20151.1
                                              • mad17
                                              		 2119529 - 2120374 (-) Acyltransferase
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                                                • ACTIVE_1789
                                                • QKG20152.1
                                                • mad16
                                                		 2120371 - 2121399 (-) 3-oxoacyl-ACP synthase
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                                                  • ACTIVE_1790
                                                  • QKG20153.1
                                                  • mad15
                                                  		 2121463 - 2122143 (-) SAM-dependent methyltransferase
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                                                    • ACTIVE_1791
                                                    • QKG20154.1
                                                    • mad14
                                                    		 2122145 - 2123272 (-) glyceryl transferase
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                                                      • ACTIVE_1792
                                                      • QKG20155.1
                                                      • mad13
                                                      		 2123317 - 2124405 (-) 2-hydroxy-3-oxopropionyl-S-ACP dehydrogenase
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                                                        • ACTIVE_1793
                                                        • QKG20156.1
                                                        • mad12
                                                        		 2124434 - 2124670 (-) acyl-carrier protein
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                                                          • ACTIVE_1794
                                                          • QKG20157.1
                                                          • mad11
                                                          		 2124730 - 2125596 (-) 3-hydroxyacyl-CoA dehydrogenase
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                                                            • ACTIVE_1795
                                                            • QKG20158.1
                                                            • mad10
                                                            		 2125623 - 2127026 (-) cyclohexane 4+2 cyclase
                                                            • Mutation phenotype: <mibig.converters.shared.mibig.gene.function.MutationPhenotype object at 0x7fabd7ae4d10>
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                                                            • ACTIVE_1796
                                                            • QKG20159.1
                                                            • madAVII
                                                            		 2127068 - 2131834 (-) type I polyketide synthase
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                                                              • ACTIVE_1797
                                                              • QKG20160.1
                                                              • mad8
                                                              		 2131853 - 2132080 (-) acyl carrier protein
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                                                                • ACTIVE_1798
                                                                • QKG20161.1
                                                                • mad7
                                                                		 2132077 - 2134005 (-) glyceryl-S-ACP synthase
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                                                                  • ACTIVE_1799
                                                                  • QKG20162.1
                                                                  • mad6
                                                                  		 2134143 - 2134913 (-) type II Thioesterase
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                                                                    • ACTIVE_1800
                                                                    • QKG20163.1
                                                                    • madAI
                                                                    		 2134963 - 2149572 (-) type I polyketide synthase
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                                                                      • ACTIVE_1801
                                                                      • QKG20164.1
                                                                      • mad4
                                                                      		 2149949 - 2150806 (+) metallophosphoesterase
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                                                                        • ACTIVE_1802
                                                                        • QKG20165.1
                                                                        • mad3
                                                                        		 2150896 - 2151474 (+) phosphopantetheinyl transferase
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                                                                          • ACTIVE_1803
                                                                          • QKG20166.1
                                                                          • mad2
                                                                          		 2152037 - 2154850 (-) LuxR family ATP-dependent transcriptional regulator
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                                                                            • ACTIVE_1804
                                                                            • QKG20167.1
                                                                            • mad1
                                                                            		 2155375 - 2155767 (-) SARP family pathway specific transcriptional activator
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                                                                              • ACTIVE_1805
                                                                              • QKG20168.1
                                                                              		 2157056 - 2157955 (-) alpha/beta hydrolase fold protein
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                                                                                • ACTIVE_1806
                                                                                • QKG20169.1
                                                                                		 2158083 - 2159438 (-) amino acid transporter
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                                                                                  • ACTIVE_1807
                                                                                  • QKG20170.1
                                                                                  		 2159697 - 2160977 (-) citrate transporter
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                                                                                    • ACTIVE_1808
                                                                                    • QKG20171.1
                                                                                    		 2161084 - 2162313 (-) sodium/hydrogen exchanger
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                                                                                      • ACTIVE_1809
                                                                                      • QKG20172.1
                                                                                      		 2162486 - 2163133 (+) hypothetical protein
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                                                                                        • ACTIVE_1810
                                                                                        • QKG20173.1
                                                                                        		 2163287 - 2163544 (-) hypothetical protein
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                                                                                          • ACTIVE_1811
                                                                                          • QKG20174.1
                                                                                          		 2164037 - 2164489 (+) hypothetical protein
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                                                                                            Biosynthesis information

                                                                                            Biosynthetic modules

                                                                                            Name
                                                                                            0
                                                                                            Type
                                                                                            pks-modular
                                                                                            Genes
                                                                                            madAI
                                                                                            Substrates
                                                                                            Integrated Monomers
                                                                                            Domains
                                                                                            acyltransferase, ketosynthase, acyltransferase, carrier (ACP)
                                                                                            Name
                                                                                            1
                                                                                            Type
                                                                                            pks-modular
                                                                                            Genes
                                                                                            madAI
                                                                                            Substrates
                                                                                            Integrated Monomers
                                                                                            Domains
                                                                                            acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
                                                                                            Name
                                                                                            2
                                                                                            Type
                                                                                            pks-modular
                                                                                            Genes
                                                                                            madAI
                                                                                            Substrates
                                                                                            Integrated Monomers
                                                                                            Domains
                                                                                            acyltransferase, ketosynthase, acyltransferase, dehydratase, enoylreductase, ketoreductase, carrier (ACP)
                                                                                            Name
                                                                                            3
                                                                                            Type
                                                                                            pks-modular
                                                                                            Genes
                                                                                            madAII
                                                                                            Substrates
                                                                                            Integrated Monomers
                                                                                            Domains
                                                                                            acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
                                                                                            Name
                                                                                            4
                                                                                            Type
                                                                                            pks-modular
                                                                                            Genes
                                                                                            madAII
                                                                                            Substrates
                                                                                            Integrated Monomers
                                                                                            Domains
                                                                                            acyltransferase, ketosynthase, acyltransferase, dehydratase, enoylreductase, ketoreductase, carrier (ACP)
                                                                                            Name
                                                                                            5
                                                                                            Type
                                                                                            pks-modular
                                                                                            Genes
                                                                                            madAIII
                                                                                            Substrates
                                                                                            Integrated Monomers
                                                                                            Domains
                                                                                            acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
                                                                                            Name
                                                                                            6
                                                                                            Type
                                                                                            pks-modular
                                                                                            Genes
                                                                                            madAIV
                                                                                            Substrates
                                                                                            Integrated Monomers
                                                                                            Domains
                                                                                            acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
                                                                                            Name
                                                                                            7
                                                                                            Type
                                                                                            pks-modular
                                                                                            Genes
                                                                                            madAIV
                                                                                            Substrates
                                                                                            Integrated Monomers
                                                                                            Domains
                                                                                            acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
                                                                                            Name
                                                                                            8
                                                                                            Type
                                                                                            pks-modular
                                                                                            Genes
                                                                                            madAV
                                                                                            Substrates
                                                                                            Integrated Monomers
                                                                                            Domains
                                                                                            acyltransferase, ketosynthase, acyltransferase, dehydratase, enoylreductase, ketoreductase, carrier (ACP)
                                                                                            Name
                                                                                            9
                                                                                            Type
                                                                                            pks-modular
                                                                                            Genes
                                                                                            madAV
                                                                                            Substrates
                                                                                            Integrated Monomers
                                                                                            Domains
                                                                                            acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
                                                                                            Name
                                                                                            10
                                                                                            Type
                                                                                            pks-modular
                                                                                            Genes
                                                                                            madAV
                                                                                            Substrates
                                                                                            Integrated Monomers
                                                                                            Domains
                                                                                            acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
                                                                                            Name
                                                                                            11
                                                                                            Type
                                                                                            pks-modular
                                                                                            Genes
                                                                                            madAVI
                                                                                            Substrates
                                                                                            Integrated Monomers
                                                                                            Domains
                                                                                            acyltransferase, ketosynthase, acyltransferase, dehydratase, enoylreductase, ketoreductase, carrier (ACP)
                                                                                            Name
                                                                                            12
                                                                                            Type
                                                                                            pks-modular
                                                                                            Genes
                                                                                            madAVI
                                                                                            Substrates
                                                                                            Integrated Monomers
                                                                                            Domains
                                                                                            acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
                                                                                            Name
                                                                                            13
                                                                                            Type
                                                                                            pks-modular
                                                                                            Genes
                                                                                            madAVI
                                                                                            Substrates
                                                                                            Integrated Monomers
                                                                                            Domains
                                                                                            acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
                                                                                            Name
                                                                                            14
                                                                                            Type
                                                                                            pks-modular
                                                                                            Genes
                                                                                            madAVII
                                                                                            Substrates
                                                                                            Integrated Monomers
                                                                                            Domains
                                                                                            acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
                                                                                            Annotation changelog

                                                                                            Entry version: 2

                                                                                            Date
                                                                                            Changes
                                                                                            Submitters
                                                                                            Reviewers
                                                                                            2022-10-07
                                                                                            Update chemical activity to schema version 2.11
                                                                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                            2022-10-07
                                                                                            Changed chemical structures for compounds: SF2487.
                                                                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                            2022-10-07
                                                                                            Added NPAtlas links to compounds: SF2487.
                                                                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                            2022-10-07
                                                                                            Renamed compounds: tetromadurin to SF2487.
                                                                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                            2022-10-07
                                                                                            Synonyms added: tetromadurin for SF2487.
                                                                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                                                            Entry version: 1

                                                                                            Date
                                                                                            Changes
                                                                                            Submitters
                                                                                            Reviewers
                                                                                            2022-09-15
                                                                                            Entry added
                                                                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                            2022-09-15
                                                                                            Fix thioesterase ID
                                                                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                            2022-09-15
                                                                                            Cleaned up synonym list
                                                                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                            Detailed domain annotation
                                                                                            Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
                                                                                            A domain glossary is available here, and an explanation of the visualisation is available here.
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