BGC0002288: ashimide A biosynthetic gene cluster from Streptomyces sp. NA03103
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Location: 1,746,781 - 1,815,669 nt. (total: 68,889 nt).
This entry is originally from NCBI GenBank CP054920.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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General
Compounds
Genes
Biosynthesis
History
KnownClusterBlast
NRPS/PKS domains
General information about the BGC
MIBiG accession BGC0002288
Short description ashimide A biosynthetic gene cluster from Streptomyces sp. NA03103
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: unknown
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
Loci
CP054920.1
1764340 - 1795669
via Heterologous expression, Enzymatic assays
Compounds
  • ashimide A
  • ashimide B
Species Streptomyces sp. NA03103 [taxonomy]
References
Chemical products information
ashimide A Evidence: NMR [2]
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C19H20N4O10
Chemical database entries
NPAtlas
ashimide B Evidence: NMR [2]
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C19H19Cl1N4O9
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • HUT15_07560
  • QKW60381.1
 1764358 - 1765065 (+) response regulator transcription factor
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    • HUT15_07565
    • QKW60382.1
    		 1765062 - 1766510 (+) HAMP domain-containing histidine kinase
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      • HUT15_07570
      • QKW60383.1
      		 1766781 - 1770011 (-) amino acid adenylation domain-containing protein
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        • HUT15_07575
        • QKW60384.1
        		 1770008 - 1771360 (-) serine hydroxymethyltransferase
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          • HUT15_07580
          • QKW60385.1
          		 1771514 - 1772980 (+) MFS transporter
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            • HUT15_07585
            • QKW60386.1
            		 1773071 - 1773613 (+) DUF1697 domain-containing protein
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              • HUT15_07590
              • QKW60387.1
              		 1773645 - 1774841 (-) 3-deoxy-7-phosphoheptulonate synthase
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                • HUT15_07595
                • QKW65597.1
                		 1774851 - 1776710 (-) chorismate-binding protein
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                  • HUT15_07600
                  • QKW60388.1
                  		 1776833 - 1778209 (-) cytochrome P450
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                    • HUT15_07605
                    • QKW60389.1
                    		 1778260 - 1778979 (-) flavodoxin family protein
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                      • HUT15_07610
                      • QKW60390.1
                      		 1778976 - 1780217 (-) cytochrome P450
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                        • HUT15_07615
                        • QKW60391.1
                        		 1780387 - 1781208 (+) SAM-dependent methyltransferase
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                          • HUT15_07620
                          • QKW60392.1
                          		 1781435 - 1784704 (-) amino acid adenylation domain-containing protein
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                            • HUT15_07625
                            • QKW60393.1
                            		 1784701 - 1788696 (-) amino acid adenylation domain-containing protein
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                              • HUT15_07630
                              • QKW60394.1
                              		 1788824 - 1790008 (+) NAD(P)/FAD-dependent oxidoreductase
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                                • HUT15_07635
                                • QKW60395.1
                                		 1790034 - 1790702 (+) class I SAM-dependent methyltransferase
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                                  • HUT15_07640
                                  • QKW60396.1
                                  		 1790829 - 1791605 (-) winged helix-turn-helix domain-containing protein
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                                    • HUT15_07645
                                    • QKW60397.1
                                    		 1791867 - 1793252 (+) phenylacetate--CoA ligase family protein
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                                      • HUT15_07650
                                      • QKW60398.1
                                      		 1793249 - 1795669 (+) non-ribosomal peptide synthetase
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                                        • HUT15_07655
                                        • QKW60399.1
                                        • msrB
                                        		 1795629 - 1796036 (-) peptide-methionine (R)-S-oxide reductase MsrB
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                                          Biosynthesis information

                                          Biosynthetic modules

                                          Name
                                          Unk01
                                          Type
                                          nrps-type1
                                          Genes
                                          HUT15_07650
                                          Substrates
                                          benzoxazolinate (evidence: Structure-based inference, ATP-PPi exchange assay)
                                          Integrated Monomers
                                          Domains
                                          adenylation
                                          Name
                                          Unk02
                                          Type
                                          nrps-type1
                                          Genes
                                          HUT15_07570
                                          Substrates
                                          glycine, serine, alanine (evidence: Structure-based inference, ATP-PPi exchange assay)
                                          Integrated Monomers
                                          Domains
                                          adenylation
                                          Name
                                          Unk03
                                          Type
                                          nrps-type1
                                          Genes
                                          HUT15_07620
                                          Substrates
                                          glycine (evidence: Structure-based inference, ATP-PPi exchange assay)
                                          Integrated Monomers
                                          Domains
                                          adenylation
                                          Name
                                          Unk04
                                          Type
                                          nrps-type1
                                          Genes
                                          HUT15_07625
                                          Substrates
                                          alanine, 3-chloro-N-methylalanine, N-methylserine (evidence: Structure-based inference, ATP-PPi exchange assay)
                                          Integrated Monomers
                                          Domains
                                          adenylation
                                          Annotation changelog

                                          Entry version: 2

                                          Date
                                          Changes
                                          Submitters
                                          Reviewers
                                          2024-04-30
                                          MIBiG v4 annotathon
                                          • (ID: 5SRAIADCX5E626OGO7LYHFZD)
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                          Entry version: 1

                                          Date
                                          Changes
                                          Submitters
                                          Reviewers
                                          2022-09-15
                                          Entry added
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          2022-09-15
                                          Added NRP substrate specificities
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
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