MIBiG Community Annotation Submission Form - Step 3: NRPS/PKS Module Info

Please consider the following instructions when filling in the table below:
  • If your gene cluster does not contain PKS/NRPS modules, just click 'Skip this step'.
  • All the information items that are preloaded are predictions. Please check them carefully and adjust them to match the true situation as described in the literature and verified by experiments.
  • Please make sure you fill out all columns; there is a scroll bar that allows you to move to columns in the table that are placed further to the right.
  • Module numbers: Counting starts from 1 at the start of the assembly-line, and includes loading modules (which are numbered '0'). If a module is split across two genes, please re-indicate the same module at each gene, but only with the domains present in that gene. Every module with at least an substrate-selecting domain (A / AT / CAL) is included in the count. If a module is split over two genes, the same number can be re-used for the domain(s) in the second gene. For hybrids, both PKS and NRPS modules are included in the counting. If this PKS/NRPS module is not part of a main assembly-line for producing this compound or if the PKS/NRPS complex is too noncanonical to describe in a linear fashion, please enter 'x'. The same applies for monomodular precursor synthases such as MSAS synthase, which may be encoded within larger multi-modular NRPS/PKS-encoding gene clusters. If there are multiple assembly-lines involved in synthesis of the main product, these can be indicated as 'A1, A2, A3, etc.' and 'B1, B2, B3'. Similarly, if the assembly-line branches at a later stage to make multiple products, the shared part can be called e.g. '1, 2, 3' and the split part 'A4, A5' and 'B4, B5'. Up to four parallel assembly-lines are supported (with letters A-D).
  • Core domains: Please use standard abbreviations, separated by commas: AT (acyltransferase), KR (ketoreductase), DH (dehydratase), ER (enoylreductase), KS (ketosynthase), T (thiolation), CAL (Co-A ligase), C (condensation), A (adenylation), E (epimerization). Do not include custom modifying domains, but select these in the next column.
  • Modifying domains: If multiple types are needed, use the exact same wording as in the drop-down menu for each descriptor, but separate them by commas: e.g., "Methylation, Oxidation".
  • KR domain activity / stereochemistry: A-group / B-group according to Caffrey, ChemBioChem 2003.
  • AT / CAL / A domain specificity: Enter 'None' if no AT-domain present (e.g., for trans-AT modules) or module is skipped. Enter 'Unknown' if specificity not known. Please use input identical to the auto-completed suggestion whenever possible. If the specificity is promiscuous (multiple), please enter all specificities separated by a forward slash (" / "), e.g. "Alanine / Glycine / Valine".
  • Evidence for substrate specificity: Choose 'None' if substrate specificity not known or not applicable. Please select the strongest level of evidence.
  • C domain subtype: See Rausch et al. (2007) BMC Evolutionary Biology 7:78. Epimerization domains are not counted as C domains, but as a separate domain type. Epimerized: please tick this box if selected amino acid is epimerized to a D-enantiomer by an epimerization domain or epimerase.

MIBiG accession: