BGC0000014 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000014 (Sorangium cellulosum)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS-like	1	100,708	ambruticin	PKS	100%

BGC0000014 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 100,708 nt. (total: 100,708 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 100,709
Label		BGC0000014

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000014	1.47	Polyketide	ambruticin	Sorangium cellulosum
BGC0001163	0.68	Polyketide	1-heptadecene	Moorea producens 3L
BGC0000043	0.66	Polyketide	curacin A	Moorea producens 19L
BGC0001162	0.63	Polyketide	1-heptadecene	Moorea producens JHB
BGC0001161	0.62	Polyketide	1-heptadecene	Moorea bouillonii PNG
BGC0001276	0.62	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001164	0.58	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000041	0.56	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000122	0.53	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0000080	0.53	Polyketide	jerangolid A, jerangolid D	Sorangium cellulosum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000014	1.00	Polyketide	ambruticin	Sorangium cellulosum
BGC0000080	0.73	Polyketide	jerangolid A, jerangolid D	Sorangium cellulosum
BGC0000043	0.70	Polyketide	curacin A	Moorea producens 19L
BGC0001164	0.69	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001163	0.69	Polyketide	1-heptadecene	Moorea producens 3L
BGC0000122	0.68	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0000996	0.66	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0001068	0.65	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0001273	0.65	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001276	0.65	Polyketide	6-methylsalicyclic acid	Aspergillus terreus

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 91724:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal) + (Me-ohmal - redmal) + (Me-ccmal - ccmal) + (ccmal) + (Me-ccmal - ccmal) + (mal) + (pk)
Non-colinear ordering:: ambA + ambB + ambC + ambD + ambE + ambF + ambH

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ambA

PKS_AT (562..859): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Malonyl-CoA: 83.3%; Methoxymalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 186.9
Ethylmalonyl-CoA: 177.1
Methoxymalonyl-CoA: 154.5
2-Methylbutyryl-CoA: 120.6
Isobutyryl-CoA: 103.5
Malonyl-CoA: 99.0
Propionyl-CoA: 98.2
trans-1,2-CPDA: 85.1
CHC-CoA: 76.0
Benzoyl-CoA: 56.5
fatty_acid: 52.2
Acetyl-CoA: 49.6
3-Methylbutyryl-CoA: 24.2
inactive: 19.5

ambB

PKS_AT (570..867): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 203.0
Ethylmalonyl-CoA: 191.7
Methoxymalonyl-CoA: 163.8
2-Methylbutyryl-CoA: 131.3
Malonyl-CoA: 109.9
Propionyl-CoA: 105.8
trans-1,2-CPDA: 98.7
Isobutyryl-CoA: 96.2
CHC-CoA: 86.8
Acetyl-CoA: 60.1
fatty_acid: 59.5
Benzoyl-CoA: 54.8
inactive: 29.5
3-Methylbutyryl-CoA: 29.1

PKS_AT (2056..2356): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.8
inactive: 116.0
Methoxymalonyl-CoA: 72.9
Methylmalonyl-CoA: 62.5
Isobutyryl-CoA: 41.3
Propionyl-CoA: 38.4
Benzoyl-CoA: 29.6
Ethylmalonyl-CoA: 26.6
2-Methylbutyryl-CoA: 26.3
Acetyl-CoA: 23.7
CHC-CoA: 11.7
trans-1,2-CPDA: 11.0
fatty_acid: 10.2
3-Methylbutyryl-CoA: 0.0

ambC

PKS_AT (571..868): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 205.8
Ethylmalonyl-CoA: 201.2
Methoxymalonyl-CoA: 171.1
2-Methylbutyryl-CoA: 132.1
Propionyl-CoA: 114.3
Isobutyryl-CoA: 113.1
Malonyl-CoA: 111.2
trans-1,2-CPDA: 101.6
CHC-CoA: 94.3
Benzoyl-CoA: 72.0
Acetyl-CoA: 69.8
fatty_acid: 50.2
inactive: 34.9
3-Methylbutyryl-CoA: 34.2

PKS_AT (2350..2651): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.8
inactive: 116.0
Methoxymalonyl-CoA: 72.9
Methylmalonyl-CoA: 62.5
Isobutyryl-CoA: 41.3
Propionyl-CoA: 38.4
Benzoyl-CoA: 29.6
Ethylmalonyl-CoA: 26.6
2-Methylbutyryl-CoA: 26.3
Acetyl-CoA: 23.7
CHC-CoA: 11.7
trans-1,2-CPDA: 11.0
fatty_acid: 10.2
3-Methylbutyryl-CoA: 0.0

ambD

PKS_AT (580..881): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.8
inactive: 137.6
Methoxymalonyl-CoA: 72.6
Methylmalonyl-CoA: 60.9
Propionyl-CoA: 31.1
Isobutyryl-CoA: 28.3
Ethylmalonyl-CoA: 24.2
Acetyl-CoA: 23.9
trans-1,2-CPDA: 22.0
Benzoyl-CoA: 18.8
CHC-CoA: 15.5
2-Methylbutyryl-CoA: 12.7
fatty_acid: 10.3
3-Methylbutyryl-CoA: 0.0

ambE

PKS_AT (570..865): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 198.9
Ethylmalonyl-CoA: 190.3
Methoxymalonyl-CoA: 158.7
2-Methylbutyryl-CoA: 123.8
Malonyl-CoA: 105.7
Propionyl-CoA: 98.7
Isobutyryl-CoA: 97.0
trans-1,2-CPDA: 95.0
CHC-CoA: 83.1
Acetyl-CoA: 65.8
Benzoyl-CoA: 59.7
fatty_acid: 52.6
3-Methylbutyryl-CoA: 31.0
inactive: 29.5

PKS_AT (2342..2643): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.9
inactive: 121.6
Methoxymalonyl-CoA: 70.9
Methylmalonyl-CoA: 61.0
Isobutyryl-CoA: 32.4
Ethylmalonyl-CoA: 29.2
Propionyl-CoA: 29.0
Benzoyl-CoA: 27.0
Acetyl-CoA: 23.0
2-Methylbutyryl-CoA: 18.0
CHC-CoA: 14.4
trans-1,2-CPDA: 13.6
fatty_acid: 10.1
3-Methylbutyryl-CoA: 0.0

ambG

CAL_domain (53..497): pk

minowa: NH2

Prediction, score:

NH2: 12.3
shikimic_acid: 11.8
AHBA: 7.7
Acetyl-CoA: 0.8
fatty_acid: 0.0

ambH

PKS_AT (561..857): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 66.7%; Propionyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 135.0
Malonyl-CoA: 125.5
Methoxymalonyl-CoA: 115.5
Isobutyryl-CoA: 114.8
Ethylmalonyl-CoA: 83.6
Acetyl-CoA: 79.7
Propionyl-CoA: 79.5
trans-1,2-CPDA: 68.1
Benzoyl-CoA: 64.4
2-Methylbutyryl-CoA: 63.2
inactive: 57.4
CHC-CoA: 41.0
3-Methylbutyryl-CoA: 37.4
fatty_acid: 35.9