LOCUS       BGC0000016              5811 bp    DNA     linear   BCT 28-NOV-2004
DEFINITION  Streptomyces nodosus amphotericin biosynthetic gene cluster, partial
            sequence.
ACCESSION   BGC0000016
VERSION     BGC0000016
KEYWORDS    .
SOURCE      Streptomyces nodosus
  ORGANISM  Streptomyces nodosus
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 14:36:01
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5811
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /db_xref="taxon:40318"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces nodosus"
     subregion       1..5811
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000016"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..5811
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             227..1090
                     /NRPS_PKS="Domain: Thioesterase (58-281). E-value: 7.5e-60.
                     Score: 195.0. Matches aSDomain:
                     nrpspksdomains_AAV48834.1_Thioesterase.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1004:thioesterase (Score: 252; E-value: 7e-77)"
                     /gene_kind="biosynthetic-additional"
                     /note="AmphE"
                     /product="Discrete thioesterase"
                     /protein_id="AAV48834.1"
                     /transl_table=11
                     /translation="MTVHSSPRSPLEPGAGTTRTSGSEGAGARQGTPATPRTPSAEESL
                     WARCFHPAPSRSTQLYCFPHAGGSASFYFPVSAQLSSVAEVFAVQYPGRQDRRKEVSPR
                     DIATLADQLYDALGPALRARPTTFFGHSMGATLAFEVARRLEADGGELVRLFASGRRAP
                     STVRDEKVHRLSDEGIVRELKLLAGTDTALLGDEEILRMILPAIRSDYEAIETYRCAPD
                     VTVRTPITVLTGDSDPKTSMAEAEAWRGHTTGDFDLQVYPGGHFYLTAEAPAVIALLRK
                     HLTGQG"
     aSDomain        401..1069
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AAV48834.1_Thioesterase.1"
                     /evalue="7.50E-60"
                     /label="AAV48834.1_Thioesterase.1"
                     /locus_tag="AAV48834.1"
                     /protein_end="281"
                     /protein_start="58"
                     /score="195.0"
                     /tool="antismash"
                     /translation="QLYCFPHAGGSASFYFPVSAQLSSVAEVFAVQYPGRQDRRKEVSP
                     RDIATLADQLYDALGPALRARPTTFFGHSMGATLAFEVARRLEADGGELVRLFASGRRA
                     PSTVRDEKVHRLSDEGIVRELKLLAGTDTALLGDEEILRMILPAIRSDYEAIETYRCAP
                     DVTVRTPITVLTGDSDPKTSMAEAEAWRGHTTGDFDLQVYPGGHFYLTAEAPAVIALLR
                     K"
     CDS_motif       599..661
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AAV48834.1_0001"
                     /evalue="2.60E-08"
                     /label="NRPS-te1"
                     /locus_tag="AAV48834.1"
                     /protein_end="145"
                     /protein_start="124"
                     /score="26.3"
                     /tool="antismash"
                     /translation="PTTFFGHSMGATLAFEVARRL"
     CDS             1484..4153
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1149:LuxR family
                     transcriptional regulator (Score: 429.5; E-value:
                     5.2e-130)"
                     /gene_kind="regulatory"
                     /note="AmphRVI"
                     /product="Similar to regulatory protein"
                     /protein_id="AAV48835.1"
                     /transl_table=11
                     /translation="MQHDAAYGRDNELTALLARAAAASRGETGSVVLTAPSGYGKSVLL
                     DTLLRAPACRGMRVLRGRCREAATGAYTGLRALFAPARAPAPDHPEATAGTDGPAVFLP
                     DPGDGRRFTPASAYPVFRRLSEHVVRLTADRPLLLVLDDAHHCDEHSLRWLDFLLRQGA
                     GRPLLVLLARRSGAGLRAPHAWTTVLAHPVVSVMPLGPLPTDAVGGLAEQVYGRPVHHR
                     LPECVAAVTGGNPRNVVRLLRELRRRGAAPDEAGAHWAMELGGGMAARSVPRLLERESP
                     EVHSVATAIALLGSVQPLDHVAVLADVDRVRTEEAIAALRHAGALTPDGTDIVHEAVRT
                     ALLEPLGTERTAELRARAALLLSDVGRPAEEAARHLMRLPDAREPWMTAVLRAAAARAE
                     QRGAFAEAAGYLRRVLDAEPDDTDVLLRLALATAPDDPPSAVPLFRAALAGTTDTLARA
                     GIAVQYAMACLSLPLPAVCRAEIQGALEDARDVLASGAETDTRLRDQVASVLLLTGRRE
                     TGAVVREHRATATAPYRPRTDRLQTEGLAALRTALTGHSRPAALEGARRVLAASAPQQP
                     LWLLFTAAVTLGLADETTEAHRALDTMVRRTSDSQTARGRLLAGSLRSYLLHRDGSVRD
                     AAAEARSVFRTECQNPGDARLVAARAVLASVLVDRGEPRQAERLLSRTGRVEPHRSALV
                     SLLSLHALARARWAAGSPVQALRLLRECGWAQESAGVLNPVVTPWWADTCLILAGLRRS
                     VEGRELAEQGAERARRWGTPRALGLAALAEGVLTPGRAGLDLLTESAHQLSGSPAVIEY
                     ARAEHVLGQALLRYGDEAGARERLRRAAGIAGGCGALALAKSARRLLVSAGAAWAMPSG
                     YRPEVCSPSPSRRWPNRR"
     misc_feature    3953..3955
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     CDS             complement(4234..5727)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1038:phenylalanine-specific permease (Score: 435.6;
                     E-value: 2.8e-132)"
                     /gene_kind="biosynthetic-additional"
                     /note="ORF10"
                     /product="Amino acid permease"
                     /protein_id="AAV48836.1"
                     /transl_table=11
                     /translation="MTPQPPPPKADNGQAGRTGPTSPAEHGSGLQAGLKNRHLSMIAIG
                     GVIGAGLFVGSSSGIATAGPGILLSYALVGTLVVLVMRMLGEMSAANPTSGSFSAHADR
                     ALGSWAGFSIGWLYWFFWVVVLAVEATAGAAILSSWVPAVPQWGWALIVMIVLTATNLI
                     SVGSYGEFEFWFAGIKVVAISIFIVIGLLAVFGVLPGVHADKAGLGNLTDHGGFLPHGL
                     SAIFTGVLLVVFSFMGSEIATLAAGESEDPQRAVTKATNSIIWRIGVFYLGSVFVVVTL
                     LPWNDKAIAEKGSYVAALDSLGIAHAGEIMKFIVLTSVLSCLNSGLYTASRMAFSLGER
                     GDAPKAFARTNSRGVPMAAILSSVVFGFVAVFFNYRFPDSVFLFLVNSSGAVALFVWLV
                     ICFSQLRLRRIIQSEAPEKLIVKMWLYPYLTWAAAFLIILVFGYMLTDTEHDGRETVLL
                     SMLVAVIVLAVAFIKQRVEGGRAKATEPAAAEAEKVSAG"
ORIGIN
        1 ggatccgccc gtcgtgcgga cgtccgccga tcccaggtgc cgcacacgag cgggacgacc
       61 ccgggcaagg ggccgtccgg tcctcggcat cgctgaagcc cctgcacggg caccatgtcg
      121 tccgccggcg ggacggcgga tcgggcgagc gcggcagtcc tcgccccgca cacgccgcat
      181 gtcaccgccg gcaccgtgcc ggcgctcacg aaagggccga gaaagcatga ccgtccacag
      241 cagcccgagg agtccgctcg agccgggcgc gggaacgacc cgcacatccg gttccgaggg
      301 ggccggagct cggcaaggta ctccggccac gccccggacg ccgtccgccg aggagagtct
      361 gtgggcccgg tgcttccacc cggccccttc ccgctccacc cagctctact gcttcccgca
      421 cgcgggcggc tcggcgtcct tctacttccc ggtgtccgca cagctgtcct cggtggccga
      481 ggtattcgcg gtgcagtacc cgggacggca ggaccggcgc aaggaagtgt cgccccggga
      541 catcgccacc ctcgccgacc agctctacga cgccctcggc cccgccctca gggcgcggcc
      601 gaccacgttc ttcgggcaca gcatgggcgc caccctggcc ttcgaggtgg cccgccggct
      661 ggaggccgac ggcggtgaac tcgtccggtt gttcgcgtcc ggtcggcgcg cgccgtcgac
      721 cgtccgcgac gagaaggtgc accgcctctc cgacgagggc atcgtgcggg agctgaagct
      781 ccttgcaggc accgacaccg cgctgctcgg cgacgaggag atcctgcgca tgatcctgcc
      841 cgccatccgc agcgactacg aggcgatcga gacctatcgg tgcgcccccg acgtcaccgt
      901 ccgcaccccg atcaccgtcc tcaccggcga cagcgacccc aagacctcga tggccgaggc
      961 cgaggcgtgg cgcggccaca ccaccggcga cttcgacctc caggtctacc ccggaggcca
     1021 cttctacctg acggcggagg cccccgcggt catcgccttg ctccggaagc acctcaccgg
     1081 gcagggctag cgccccgaca ggcaacgttc gccccgtcgc gacgcccggc acgccttctt
     1141 gccgcaccgg ccgcaagccc gagtacgtcc agtacgaggg cttccggccg gcacaccgag
     1201 agcatgcacc ggccaccgct ccctcacggg cagacgttgc ctgccgcggt accggcgtct
     1261 gccgttccgc ccctcgtcag gccacggacg gcgggcggaa cggacggcct ccagcggggc
     1321 gggcagatgc cggaggagtc ccgtgccggt gtgccgcacg gccgagtcgc gtgtgcggct
     1381 cagtcctgcg gtccaccgga ccgcccgacg cggagggccg gtgtgtcgag ggcggcgggg
     1441 gctgtgtgcg caaccaaggg aaaacgaacc aggggaagtt ttcgtgcagc acgacgcggc
     1501 atacggccgc gacaacgaac tgaccgctct cctcgcccgg gcggccgccg catcccgcgg
     1561 tgagacgggg tccgtggttc tgaccgcacc gtccggctac ggcaagagcg ttctcctcga
     1621 caccctgctg cgtgcccccg cctgccgggg catgagggta ctgcggggcc gctgccgaga
     1681 ggcggcgacg ggcgcctaca ccggactgcg ggcgttgttc gcaccggcga gagcaccggc
     1741 tccggaccac ccggaagcga cggcgggcac ggacggcccg gccgtgttcc tgcccgaccc
     1801 tggggacggc cggagattca cccccgcctc cgcctacccg gtgttccgcc ggctcagcga
     1861 gcacgtggtc cggctgacgg ccgaccggcc gctgctgctg gtgctcgacg acgcccacca
     1921 ctgcgacgaa cactccctgc gctggctgga cttcctgctg cggcagggag caggacgccc
     1981 gctgctggtg ctcctcgccc ggcgctccgg ggccggcctg cgcgctcccc acgcctggac
     2041 gaccgtcctc gcccacccgg tcgtgtccgt gatgcccctc ggtccgctcc ccaccgacgc
     2101 cgtcggcggc ctcgccgaac aggtctacgg cagaccggtg caccatcggc tccccgaatg
     2161 cgtggccgcg gtcaccggcg gcaacccacg gaacgtcgta cgactgctgc gtgagctgcg
     2221 acggcggggc gccgccccgg acgaggccgg tgcacactgg gcgatggagc tcggcggcgg
     2281 catggcggcc aggtcggtgc cacggctgct ggagcgcgag tcccccgagg tccacagcgt
     2341 cgccacggcg atcgcactgc tcgggagcgt gcagccgctg gaccatgtgg cggtcctggc
     2401 ggatgtggac cgcgtgcgaa ccgaggaggc gatcgcggct ctgcgccatg cgggagccct
     2461 caccccggac ggcaccgaca tcgtgcacga agccgtacgc accgcgctgc tcgaaccgct
     2521 cggcaccgag cggacggccg aactgcgcgc ccgcgccgcg ctgttgctca gcgatgtcgg
     2581 ccggcccgcg gaagaggcgg cccggcatct gatgcggctg cccgatgccc gggaaccgtg
     2641 gatgaccgcg gtgctgcgcg ccgccgccgc ccgggcggaa cagcggggcg ccttcgccga
     2701 ggcggccggc tatctgcgcc gggtcctgga cgcggagccc gacgacaccg acgtactgct
     2761 gcggctcgcg ctcgccacgg cccccgacga cccgccgagc gccgttccgc tgttccgcgc
     2821 ggcactcgcc ggcacgaccg acaccctcgc gcgcgccggt atcgcggtcc agtacgccat
     2881 ggcctgcctg tccctgccgc tccccgccgt ctgccgggcg gagatccagg gggccctgga
     2941 ggacgcccgg gacgtcctcg cgtccggcgc cgagaccgac acacgactgc gcgaccaggt
     3001 ggcgtccgtc ttgctgctga cgggccgacg ggagaccggc gccgtggtgc gggaacaccg
     3061 cgccacggcg acggcaccgt accggccgcg caccgaccgg ctccagacgg agggcctcgc
     3121 cgccctgcgc accgcgctga ccggccactc caggccggcc gccctggaag gcgcccggcg
     3181 tgtgctggcg gcgtccgccc cccagcagcc gctgtggctg ctgttcaccg ccgccgtcac
     3241 cctgggcctc gcggacgaga ccacggaagc gcaccgggcg ctggacacca tggtgcggcg
     3301 caccagcgac agccagacgg cccggggccg gctgctcgcc gggtccctgc ggtcctatct
     3361 gttgcaccgg gacggctcgg tgcgcgacgc cgccgcggaa gcccggtcgg tgttccgcac
     3421 ggagtgtcag aaccccggtg acgcacggct ggtggcggca cgagcggtgc tggcgtcggt
     3481 gctcgtggac cgcggcgagc cgcgccaggc cgaacggctg ctgtcccgga cgggccgcgt
     3541 cgagccgcac cgctccgccc tcgtctccct gctgtctctg cacgcccttg cccgggcccg
     3601 ctgggcggcg ggcagtcccg tgcaggcgct gcggctgctg cgcgagtgcg gctgggcgca
     3661 ggagagtgcc ggtgtactga acccggtggt caccccgtgg tgggcggaca cctgtctgat
     3721 cctggcgggc ctgcgccgat ccgtggaggg cagggaactg gccgaacagg gcgccgaacg
     3781 cgcccggcgc tggggaacac cccgggccct cgggctcgcc gcgctcgcgg agggcgtcct
     3841 cacccccggc cgggccggcc tcgacctgct caccgagtcg gcccaccagc tttccgggtc
     3901 gcccgcggtc atcgagtacg cccgcgccga acacgtactg ggccaggccc tcttacgcta
     3961 cggcgacgag gccggggccc gggagcggct gcgcagggcg gccggcatcg cgggcggctg
     4021 cggggcgctc gccctcgcga agtcggcccg tcgcctgctg gtctccgccg gggccgcatg
     4081 ggccatgccg tccgggtacc ggccggaggt ctgctcgccg tcaccgagcc gaaggtggcc
     4141 gaaccggcgg tgaccggtcc ctgccgcccg tacggaaggc ctccacggcc ttccgtacga
     4201 acaggcgccg cacggggtgc cggcctgggg ccgtcagccg gccgagacct tctccgcctc
     4261 ggcggcggcg ggctccgtgg ccttcgcccg gccgccctcg accctctgct tgatgaaggc
     4321 gaccgccagc acgatcaccg cgaccagcat cgacagcagc acggtctcgc ggccgtcgtg
     4381 ttcggtgtcg gtcagcatgt agccgaacac gagaatgatc agaaacgccg ccgcccaggt
     4441 cagatacggg tacagccaca tcttcacgat cagcttctcc ggcgcctccg actggatgat
     4501 cctgcgcagg cgcagctgcg agaagcagat caccagccac acgaacaggg cgaccgcgcc
     4561 cgaggagttg accaggaaga ggaagacgga gtccgggaac cggtagttga agaagacggc
     4621 cacgaagccg aacacgacgg acgacaggat cgcggccatc ggcacaccac ggctgttggt
     4681 ccgcgcgaac gccttcggcg cgtccccgcg ctcgccgagc gagaaggcca tccgggaggc
     4741 ggtgtagaga ccggagttga gacaggacag caccgaggtc agcacgatga atttcatgat
     4801 ctcaccggcg tgcgcgatac cgagggagtc cagggcggcg acataggagc ccttctcggc
     4861 gatcgccttg tcgttccagg ggagcagggt gacgaccacg aagaccgagc cgaggtagaa
     4921 gacgccgatg cgccagatga tgctgttggt cgccttggtg acggcgcgct gagggtcctc
     4981 cgactcgccg gcggccagcg tcgcgatctc actgcccatg aaggagaaga cgacgagcag
     5041 cacaccggtg aagatcgcgc tgagcccgtg cggcaggaag ccgccgtggt cggtgaggtt
     5101 gccgagcccg gccttgtcgg cgtgcacacc gggcagcacc ccgaagacgg ccagcagacc
     5161 gatgacgatg aagatgctga tcgccacgac cttgattccg gcgaaccaga actcgaactc
     5221 gccgtaggag ccgacggaga tcaggttggt ggcggtcagc acgatcatga cgatcagggc
     5281 ccagccccac tggggcacgg cgggtaccca gctggagagg atcgcggcgc cggcggtcgc
     5341 ctccaccgcc agcacgacga cccagaagaa ccagtacagc cagccgatgg agaacccggc
     5401 ccagctgccg agggcgcggt cggcgtgcgc cgagaaggag ccggaggtgg ggttggcggc
     5461 ggacatctcg ccgagcatgc gcatcacgag gacgacgagc gtgccgacga gggcgtacga
     5521 cagcaggatg ccggggccgg ccgtggcgat gccggagctg gagccgacga acagtcccgc
     5581 gccgatcaca ccgccgatgg cgatcatcga cagatgacgg ttcttcaggc ccgcctgaag
     5641 ccctgaacca tgttctgcgg ggcttgtcgg gccggttcgg cctgcctggc cgttgtcagc
     5701 cttcggcggg ggcggctggg gggtcatggg gggagtcctt agtgccgggt cagcgatctc
     5761 ccgtacggag agcgcacgag ctggaccagt gaatccgagg caatgggatc c
//
