BGC0000017 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000017 ([Oscillatoria] sp. PCC 6506 (Oscillatoria sp. PCC 9029 = Lyngbya kuetzingii UTEX B 1547))

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	28,910	High	anatoxin-a/homoanatoxin-a	PKS:Type I+other:other

BGC0000017 - Region 1 - T1PKS

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Location: 1 - 28,910 nt. (total: 28,910 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 28,911
Label		BGC0000017

Tailoring functions

Oxidoreductases: dehydrogenase

anaB: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

anaE: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Hydrolases: esterase

anaA: thioesterase

smcogs: SMCOG1004: thioesterase

Isomerases: mutase

ACR33086.1: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: ligase synthase

anaC: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

anaF: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

anaG: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000017.5	0.99	PKS, other (other)	anatoxin-a, homoanatoxin-a	[Oscillatoria] sp. PCC 6506
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.67	PKS	asperlactone	Aspergillus ochraceus
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002175.3	0.67	PKS	YWA1	Aspergillus oryzae RIB40
BGC0000107.3	0.67	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001284.4	0.67	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000017.5	0.99	PKS, other (other)	anatoxin-a, homoanatoxin-a	[Oscillatoria] sp. PCC 6506
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.67	PKS	asperlactone	Aspergillus ochraceus
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002175.3	0.67	PKS	YWA1	Aspergillus oryzae RIB40
BGC0000107.3	0.67	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001284.4	0.67	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 28910:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (redmal) + (ccmal) + (Me-mal)
Non-colinear ordering:: anaE + anaF + anaG

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

anaC: Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (11..433): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLFYLALVCK
Nearest Stachelhaus code(s):: Pro DLFYLALVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

anaE: mal

PKS_AT (551..848): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 177.6
inactive: 170.2
Methoxymalonyl-CoA: 49.5
Methylmalonyl-CoA: 42.0
Propionyl-CoA: 28.3
Isobutyryl-CoA: 25.7
Benzoyl-CoA: 17.3
2-Methylbutyryl-CoA: 12.1
Ethylmalonyl-CoA: 10.9
CHC-CoA: 10.9
3-Methylbutyryl-CoA: 9.8
trans-1,2-CPDA: 9.4
fatty_acid: 0.0
Acetyl-CoA: 0.0

anaF: mal

PKS_AT (559..852): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 100.0%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 210.7
inactive: 188.7
Methylmalonyl-CoA: 67.6
Methoxymalonyl-CoA: 66.3
Propionyl-CoA: 52.1
2-Methylbutyryl-CoA: 39.8
Ethylmalonyl-CoA: 34.5
Isobutyryl-CoA: 33.7
Acetyl-CoA: 26.0
CHC-CoA: 25.4
Benzoyl-CoA: 23.4
trans-1,2-CPDA: 21.1
3-Methylbutyryl-CoA: 11.6
fatty_acid: 0.0

anaG: mal

PKS_AT (566..859): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 100.0%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 210.8
inactive: 185.7
Methoxymalonyl-CoA: 73.8
Methylmalonyl-CoA: 72.5
Propionyl-CoA: 51.2
Isobutyryl-CoA: 42.9
CHC-CoA: 35.3
Ethylmalonyl-CoA: 34.5
2-Methylbutyryl-CoA: 31.7
Acetyl-CoA: 24.3
Benzoyl-CoA: 22.8
trans-1,2-CPDA: 19.8
3-Methylbutyryl-CoA: 13.5
fatty_acid: 0.0

Link to PARAS substrate predictor