BGC0000018 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000018 (Streptomyces eurythermus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	42,960	Low	niphimycin C/niphimycin D/niphimycin E/17-O-methylniphimycin/niphimycin Iα/19-O-malonylniphimycin	PKS

BGC0000018 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 42,960 nt. (total: 42,960 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 42,961
Label		BGC0000018

Tailoring functions

Transferases: acylase

angAIII: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

angAIV: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Ligases: synthase

angAI: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

angAV: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000018.5	1.00	PKS	angolamycin	Streptomyces eurythermus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000166.5	0.74	PKS	tylactone	Streptomyces fradiae
BGC0000041.3	0.73	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000018.5	1.00	PKS	angolamycin	Streptomyces eurythermus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000166.5	0.74	PKS	tylactone	Streptomyces fradiae
BGC0000041.3	0.73	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 42960:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ohmal - Me-ccmal) + (ccmal) + (Me-ccmal) + (Me-ohmal - redemal) + (ohmal)
Non-colinear ordering:: angAI + angAII + angAIV + angAIII + angAV

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

angAI: mmal - mmal - mmal

PKS_AT (595..889): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 222.8
Ethylmalonyl-CoA: 215.6
Methoxymalonyl-CoA: 179.0
2-Methylbutyryl-CoA: 143.1
trans-1,2-CPDA: 126.9
Propionyl-CoA: 106.9
CHC-CoA: 97.5
Malonyl-CoA: 89.5
Isobutyryl-CoA: 85.5
Benzoyl-CoA: 81.0
Acetyl-CoA: 43.3
fatty_acid: 30.8
3-Methylbutyryl-CoA: 30.5
inactive: 25.8

PKS_AT (1594..1888): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 221.1
Ethylmalonyl-CoA: 217.9
Methoxymalonyl-CoA: 177.9
2-Methylbutyryl-CoA: 141.3
trans-1,2-CPDA: 125.9
Propionyl-CoA: 106.9
CHC-CoA: 99.5
Malonyl-CoA: 88.8
Isobutyryl-CoA: 81.0
Benzoyl-CoA: 80.4
Acetyl-CoA: 44.2
fatty_acid: 32.2
3-Methylbutyryl-CoA: 29.2
inactive: 25.5

PKS_AT (3078..3340): mmal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 79.2%; Methylmalonyl-CoA: 79.2%; Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 172.4
Methoxymalonyl-CoA: 150.0
Ethylmalonyl-CoA: 134.4
2-Methylbutyryl-CoA: 112.5
trans-1,2-CPDA: 104.6
Propionyl-CoA: 83.6
CHC-CoA: 82.0
Malonyl-CoA: 64.2
Benzoyl-CoA: 51.9
Isobutyryl-CoA: 44.7
Acetyl-CoA: 19.1
inactive: 17.9
fatty_acid: 16.3
3-Methylbutyryl-CoA: 14.1

angAII: mal

PKS_AT (641..983): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 219.5
inactive: 120.8
Methoxymalonyl-CoA: 80.4
Methylmalonyl-CoA: 70.4
Isobutyryl-CoA: 42.3
Ethylmalonyl-CoA: 30.7
Propionyl-CoA: 28.1
Acetyl-CoA: 24.8
Benzoyl-CoA: 19.2
CHC-CoA: 18.6
2-Methylbutyryl-CoA: 17.5
fatty_acid: 13.3
3-Methylbutyryl-CoA: 10.9
trans-1,2-CPDA: 10.6

angAIII: mmal - emal

PKS_AT (569..863): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 222.9
Ethylmalonyl-CoA: 214.6
Methoxymalonyl-CoA: 179.8
2-Methylbutyryl-CoA: 149.1
trans-1,2-CPDA: 126.5
Propionyl-CoA: 108.0
CHC-CoA: 95.2
Malonyl-CoA: 89.9
Isobutyryl-CoA: 87.5
Benzoyl-CoA: 78.4
Acetyl-CoA: 44.3
fatty_acid: 33.3
3-Methylbutyryl-CoA: 31.0
inactive: 29.0

PKS_AT (2148..2445): emal

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Methylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 234.6
Methylmalonyl-CoA: 207.6
Methoxymalonyl-CoA: 182.0
trans-1,2-CPDA: 159.6
2-Methylbutyryl-CoA: 148.7
Propionyl-CoA: 118.4
CHC-CoA: 107.5
Malonyl-CoA: 83.9
Isobutyryl-CoA: 71.9
Benzoyl-CoA: 61.6
Acetyl-CoA: 56.1
fatty_acid: 46.5
3-Methylbutyryl-CoA: 36.2
inactive: 17.4

angAIV: mmal

PKS_AT (557..851): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 222.3
Ethylmalonyl-CoA: 214.0
Methoxymalonyl-CoA: 176.0
2-Methylbutyryl-CoA: 140.7
trans-1,2-CPDA: 121.9
Propionyl-CoA: 103.4
CHC-CoA: 94.7
Malonyl-CoA: 88.3
Benzoyl-CoA: 76.9
Isobutyryl-CoA: 73.9
Acetyl-CoA: 42.7
fatty_acid: 33.5
3-Methylbutyryl-CoA: 23.6
inactive: 21.2

angAV: mal

PKS_AT (599..899): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 204.4
inactive: 94.0
Methoxymalonyl-CoA: 59.5
Methylmalonyl-CoA: 51.3
Propionyl-CoA: 34.8
Acetyl-CoA: 25.4
Ethylmalonyl-CoA: 19.1
CHC-CoA: 16.7
Isobutyryl-CoA: 14.9
Benzoyl-CoA: 14.0
2-Methylbutyryl-CoA: 12.6
fatty_acid: 10.7
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor