BGC0000020 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000020 (Actinosynnema pretiosum subsp. auranticum)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS-like	1	82,746	maytansine/ansamitocin P-3	PKS	100%

BGC0000020 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 82,746 nt. (total: 82,746 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 82,747
Label		BGC0000020

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000020	1.87	Polyketide	maytansine, ansamitocin P-3	Actinosynnema pretiosum subsp. auranticum
BGC0001511	1.08	Polyketide	ansamitocin P-3	Actinosynnema pretiosum subsp. pretiosum
BGC0000041	1.03	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0002341	1.03	Other	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0000840	0.83	Other	xanthomonadin I	Xanthomonas oryzae pv. oryzae
BGC0002081	0.78	Polyketide	streptoketide A, streptoketide B, streptoketide C, S2502, S2507, UWM5	Streptomyces sp. Tue6314
BGC0002171	0.77	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275	0.77	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger CBS 101883
BGC0000996	0.77	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0000887	0.77	Alkaloid	saxitoxin, gonyautoxin 2, gonyautoxin 3, toxin C2, toxin C3, gonyautoxin 5, decarbamoylsaxitoxin, decarbamoylgonyautoxin 2, decarbamoylgonyautoxin 3	Dolichospermum circinale AWQC131C

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000020	1.00	Polyketide	maytansine, ansamitocin P-3	Actinosynnema pretiosum subsp. auranticum
BGC0001511	0.77	Polyketide	ansamitocin P-3	Actinosynnema pretiosum subsp. pretiosum
BGC0000131	0.69	Polyketide	pyrrolomycin	Streptomyces sp. UC 11065
BGC0000041	0.68	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0002341	0.67	Other	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002404	0.66	Other	falcarindiol	Solanum lycopersicum
BGC0000996	0.65	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0001163	0.65	Polyketide	1-heptadecene	Moorea producens 3L
BGC0001273	0.64	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001276	0.64	Polyketide	6-methylsalicyclic acid	Aspergillus terreus

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 82746:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal - ccmal) + (Me-ohmal) + (Me-ccmal - ccmal) + (ccmxmal - mal)
Non-colinear ordering:: asmA + asmC + asmD + asmB

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 57041:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal - ccmal) + (ccmxmal - mal)
Non-colinear ordering:: asmA + asmB

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 37225 - 82746:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-ohmal) + (Me-ccmal - ccmal)
Non-colinear ordering:: asmC + asmD

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

asmB

PKS_AT (647..939): mxmal

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 87.5%; Methylmalonyl-CoA: 87.5%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 188.0
Methylmalonyl-CoA: 179.8
Ethylmalonyl-CoA: 169.5
trans-1,2-CPDA: 105.9
Malonyl-CoA: 96.2
2-Methylbutyryl-CoA: 87.4
CHC-CoA: 85.9
Propionyl-CoA: 84.2
Isobutyryl-CoA: 83.0
Acetyl-CoA: 54.1
Benzoyl-CoA: 52.3
3-Methylbutyryl-CoA: 48.7
inactive: 28.1
fatty_acid: 17.8

PKS_AT (2289..2572): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 162.1
Methoxymalonyl-CoA: 64.8
inactive: 64.0
Methylmalonyl-CoA: 54.2
Propionyl-CoA: 32.1
Ethylmalonyl-CoA: 23.9
fatty_acid: 17.2
2-Methylbutyryl-CoA: 15.1
Isobutyryl-CoA: 13.0
CHC-CoA: 11.9
Benzoyl-CoA: 11.6
trans-1,2-CPDA: 8.6
Acetyl-CoA: 5.2
3-Methylbutyryl-CoA: 0.0

asmA

CAL_domain (34..434): pk

minowa: AHBA

Prediction, score:

AHBA: 518.2
shikimic_acid: 248.9
NH2: 10.1
Acetyl-CoA: 10.0
fatty_acid: 0.0

PKS_AT (1536..1819): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 198.8
Ethylmalonyl-CoA: 183.5
Methoxymalonyl-CoA: 161.3
trans-1,2-CPDA: 121.1
2-Methylbutyryl-CoA: 113.7
Malonyl-CoA: 98.8
Propionyl-CoA: 88.3
CHC-CoA: 85.7
Isobutyryl-CoA: 74.5
Benzoyl-CoA: 62.4
inactive: 46.5
3-Methylbutyryl-CoA: 29.5
Acetyl-CoA: 27.9
fatty_acid: 24.1

PKS_AT (3572..3851): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 66.7%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 138.0
Methoxymalonyl-CoA: 51.6
inactive: 48.0
Methylmalonyl-CoA: 46.3
Ethylmalonyl-CoA: 19.5
Isobutyryl-CoA: 17.1
Benzoyl-CoA: 11.0
fatty_acid: 10.6
Propionyl-CoA: 7.2
CHC-CoA: 4.2
Acetyl-CoA: 3.8
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -1.2

asmD

PKS_AT (557..842): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 79.2%; Malonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 181.8
Ethylmalonyl-CoA: 141.0
Methoxymalonyl-CoA: 137.2
2-Methylbutyryl-CoA: 99.2
CHC-CoA: 93.0
Propionyl-CoA: 90.7
trans-1,2-CPDA: 86.0
Malonyl-CoA: 72.9
Isobutyryl-CoA: 54.6
Benzoyl-CoA: 49.7
fatty_acid: 35.8
3-Methylbutyryl-CoA: 31.0
Acetyl-CoA: 29.7
inactive: 14.4

PKS_AT (2251..2525): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 124.9
Methoxymalonyl-CoA: 60.6
Methylmalonyl-CoA: 56.3
inactive: 33.8
Propionyl-CoA: 29.9
Isobutyryl-CoA: 22.3
Ethylmalonyl-CoA: 20.3
fatty_acid: 9.8
Benzoyl-CoA: 4.8
CHC-CoA: 0.8
2-Methylbutyryl-CoA: 0.7
trans-1,2-CPDA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

asmC

PKS_AT (559..841): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 191.1
Ethylmalonyl-CoA: 164.9
Methoxymalonyl-CoA: 144.4
trans-1,2-CPDA: 104.9
Malonyl-CoA: 100.7
2-Methylbutyryl-CoA: 96.6
Propionyl-CoA: 81.1
CHC-CoA: 76.9
Isobutyryl-CoA: 65.6
Benzoyl-CoA: 56.4
inactive: 42.4
fatty_acid: 29.9
3-Methylbutyryl-CoA: 25.5
Acetyl-CoA: 23.7