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Overview
1.1

### Identified secondary metabolite regions using strictness 'relaxed'

BGC0000021 (Amycolatopsis sp. FU40)

Region | Type | From | To | Similarity Confidence | Most similar known cluster
--- | --- | --- | --- | --- | ---
Region 1 | T1PKS,transAT-PKS | 1 | 115,746 | High | apoptolidin | PKS

BGC0000021 - Region 1 - T1PKS,transAT-PKS
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
More detailed help is available here.
Download region GenBank file
Download region SVG
Location: 1 - 115,746 nt. (total: 115,746 nt) Show pHMM detection rules used
Region on contig edge.
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))

#### Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
Gene overview
MIBiG
Tailoring
MIBiG comparison
KnownClusterBlast
NRPS/PKS modules
NRPS/PKS domains
Gene/CDS overview
A brief tabular summary of genes/CDS features within the region.
Filtering the table will also search biosynthetic profiles and gene function data. If enabled, the overview will then zoom to show the area covered by the filtered selection.
Genes selected in the region drawing above will be marked in the table with an indicator to the left of the gene name.
Filter:
Automatically zoom to filtered/selected features

Identifier | Product | Length | Function | Sequence | NCBI Blast | Filter details
--- | --- | --- | --- | --- | --- | ---
|  | NT | AA |  | NT | AA |  |

No genes match the given filter
External annotation
This annotation was made externally by 'MIBiG' and not by antiSMASH

Name |  | MIBiG
Version |  | 4.0alpha

Type |  | Subregion
Location |  | 1 - 115,747
Label |  | BGC0000021

Tailoring functions
Tailoring enzymes detected in this region
Oxidoreductases: dehydrogenase oxidoreductase oxygenase
apoC: oxidoreductase

smcogs
  SMCOG1079: oxidoreductase

apoH2: oxidoreductase

smcogs
  SMCOG1079: oxidoreductase

apoI: acyl-CoA dehydrogenase

smcogs
  SMCOG1006: acyl-CoA dehydrogenase

apoK: 3-hydroxybutyryl-CoA dehydrogenase

smcogs
  SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

apoP: cytochrome P450

smcogs
  SMCOG1007: cytochrome P450

Transferases: acylase glycosyltransferase methyltransferase
apoGT1: glycosyltransferase, MGT family

smcogs
  SMCOG1062: glycosyltransferase, MGT family

apoGT2: glycosyltransferase, MGT family

smcogs
  SMCOG1062: glycosyltransferase, MGT family

apoGT3: glycosyltransferase, MGT family

smcogs
  SMCOG1062: glycosyltransferase, MGT family

apoM4: methyltransferase

smcogs
  SMCOG1089: methyltransferase

apoS10: malonyl CoA-acyl carrier protein transacylase

smcogs
  SMCOG1021: malonyl CoA-acyl carrier protein transacylase

apoS4: malonyl CoA-acyl carrier protein transacylase

smcogs
  SMCOG1021: malonyl CoA-acyl carrier protein transacylase

apoS6: malonyl CoA-acyl carrier protein transacylase

smcogs
  SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase phosphatase
apoE: FkbH like protein

smcogs
  SMCOG1256: FkbH like protein

apoS11: thioesterase

smcogs
  SMCOG1004: thioesterase

Lyases: dehydratase
apoH3: NAD-dependent epimerase/dehydratase

smcogs
  SMCOG1010: NAD-dependent epimerase/dehydratase

apoH4: NAD-dependent epimerase/dehydratase

smcogs
  SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase
apoS1: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

apoS2: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

apoS3: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

apoS5: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

apoS7: Beta-ketoacyl synthase

smcogs
  SMCOG1093: Beta-ketoacyl synthase

apoS8: Beta-ketoacyl synthase

smcogs
  SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters
Shows areas that are similar to the current region to a reference database.
Mouseover a score cell in the table to get a breakdown of how the score was calculated.The MIBiG database.
Click on an accession to open that entry in the MIBiG database.
Analysis type:

Reference | T1PKS | transAT-PKS | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | --- | ---
BGC0000021.5 |  |  | 1.61 | PKS | apoptolidin | Nocardiopsis sp. FU 40
BGC0001244.3 |  |  | 0.97 | PKS | (-)-Mellein | Parastagonospora nodorum
BGC0000041.3 |  |  | 0.92 | PKS | oronofacic acid | Pseudomonas syringae
BGC0001275.3 |  |  | 0.91 | PKS | 6-methylsalicyclic acid | Glarea lozoyensis
BGC0001276.3 |  |  | 0.91 | PKS | 6-methylsalicyclic acid | Aspergillus terreus
BGC0001273.3 |  |  | 0.90 | PKS | asperlactone | Aspergillus ochraceus
BGC0001257.3 |  |  | 0.68 | PKS | 1,3,6,8-tetrahydroxynaphthalene | Nodulisporium sp. ATCC74245
BGC0002154.2 |  |  | 0.68 | PKS | 1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene | Pyricularia oryzae 70-15
BGC0000018.5 |  |  | 0.67 | PKS | angolamycin | Streptomyces eurythermus
BGC0001284.4 |  |  | 0.67 | PKS | alternariol | Parastagonospora nodorum SN15

Reference | Aggregated | Similarity score | Type | Compound(s) | Organism
--- | --- | --- | --- | --- | ---
BGC0000021.5 |  | 0.99 | PKS | apoptolidin | Nocardiopsis sp. FU 40
BGC0001244.3 |  | 0.78 | PKS | (-)-Mellein | Parastagonospora nodorum
BGC0000041.3 |  | 0.76 | PKS | oronofacic acid | Pseudomonas syringae
BGC0001275.3 |  | 0.76 | PKS | 6-methylsalicyclic acid | Glarea lozoyensis
BGC0001276.3 |  | 0.76 | PKS | 6-methylsalicyclic acid | Aspergillus terreus
BGC0001273.3 |  | 0.75 | PKS | asperlactone | Aspergillus ochraceus
BGC0001257.3 |  | 0.67 | PKS | 1,3,6,8-tetrahydroxynaphthalene | Nodulisporium sp. ATCC74245
BGC0002154.2 |  | 0.67 | PKS | 1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene | Pyricularia oryzae 70-15
BGC0001284.4 |  | 0.67 | PKS | alternariol | Parastagonospora nodorum SN15
BGC0002191.2 |  | 0.66 | PKS | prolipyrone B, gibepyrone D | Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0
Shows clusters from the MIBiG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.
Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.
Click on an accession to open that entry in the MIBiG database.
Location:
Identifier
Identity
Coverage
Module view
Shows module structures for each candidate cluster in NRPS and PKS regions.
Genes are shown in predicted order, and are only present when containing at least one complete module.
A domain glossary is available here, and an explanation of the visualisation is available here.
Legend
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A domain glossary is available here, and an explanation of the visualisation is available here.
Selected features only
Show module domains
NRPS/PKS products
NRPS/PKS substrates
Predicted core structure(s)
Shows estimated product structure and polymer for each candidate cluster in the region. To show the product, click on the expander or the candidate cluster feature drawn in the overview.
For candidate cluster 1, location 3921 - 115746:
Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:
  (pk - ccmal - Me-ohmal - Me-ohmal) + (ccmal) + (ohmal) + (ohmal - redmal - pk) + (ccmal - pk) + (Me-ccmal - Me-ccmal) + (Me-ccmal)
Non-colinear ordering:
  apoS1 + apoS2 + apoS3 + apoS4 + apoS5 + apoS6 + apoS7 + apoS8

Direct lookup in NORINE database: strict or relaxed
Link to NORINE database query form
NRPS/PKS substrate predictions
Shows the predicted substrates for each adenylation domain and acyltransferase within genes. Each gene prediction can be expanded to view detailed predictions of each domain. Each prediction can be expanded to view the predictions by tool (and, for some tools, further expanded for extra details).
apoS10: mmal
PKS_AT (488..785): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 91.7%
  Ethylmalonyl-CoA: 83.3%
  Methoxymalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 201.2
  Ethylmalonyl-CoA: 194.1
  Methoxymalonyl-CoA: 161.2
  2-Methylbutyryl-CoA: 127.7
  trans-1,2-CPDA: 119.4
  CHC-CoA: 106.5
  Malonyl-CoA: 89.2
  Propionyl-CoA: 89.0
  Isobutyryl-CoA: 72.1
  Benzoyl-CoA: 52.8
  Acetyl-CoA: 45.9
  3-Methylbutyryl-CoA: 32.2
  inactive: 31.6
  fatty_acid: 13.4

apoS8: mmal
PKS_AT (371..662): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 91.7%
  Methoxymalonyl-CoA: 83.3%
  Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 216.9
  Ethylmalonyl-CoA: 215.5
  Methoxymalonyl-CoA: 174.2
  2-Methylbutyryl-CoA: 157.8
  trans-1,2-CPDA: 146.6
  Propionyl-CoA: 118.3
  Malonyl-CoA: 109.3
  CHC-CoA: 102.4
  Isobutyryl-CoA: 85.9
  Benzoyl-CoA: 73.2
  Acetyl-CoA: 61.3
  fatty_acid: 44.9
  inactive: 29.8
  3-Methylbutyryl-CoA: 29.4

apoS7: mmal - mmal
PKS_AT (559..848): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 95.8%
  Methoxymalonyl-CoA: 87.5%
  Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 215.6
  Ethylmalonyl-CoA: 214.8
  Methoxymalonyl-CoA: 173.2
  2-Methylbutyryl-CoA: 160.4
  trans-1,2-CPDA: 136.6
  Propionyl-CoA: 119.1
  CHC-CoA: 108.1
  Malonyl-CoA: 103.4
  Isobutyryl-CoA: 80.1
  Benzoyl-CoA: 68.5
  Acetyl-CoA: 54.1
  3-Methylbutyryl-CoA: 44.5
  fatty_acid: 37.4
  inactive: 26.9

PKS_AT (2259..2556): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 218.6
  Ethylmalonyl-CoA: 217.0
  Methoxymalonyl-CoA: 170.2
  2-Methylbutyryl-CoA: 150.0
  trans-1,2-CPDA: 148.3
  Propionyl-CoA: 127.2
  Malonyl-CoA: 112.1
  CHC-CoA: 107.0
  Isobutyryl-CoA: 83.4
  Benzoyl-CoA: 56.8
  Acetyl-CoA: 51.7
  fatty_acid: 41.9
  inactive: 38.9
  3-Methylbutyryl-CoA: 35.2

apoS6: mal - pk
PKS_AT (541..820): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 79.2%
  Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 201.9
  inactive: 100.9
  Methoxymalonyl-CoA: 65.4
  Methylmalonyl-CoA: 59.6
  Propionyl-CoA: 42.7
  Benzoyl-CoA: 28.1
  Acetyl-CoA: 26.7
  Ethylmalonyl-CoA: 25.9
  2-Methylbutyryl-CoA: 22.8
  Isobutyryl-CoA: 21.0
  trans-1,2-CPDA: 14.6
  CHC-CoA: 12.7
  fatty_acid: 12.3
  3-Methylbutyryl-CoA: 0.0

PKS_AT (2274..2564): pk
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 95.8%
  Methoxymalonyl-CoA: 91.7%
  Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:
  Ethylmalonyl-CoA: 217.2
  Methylmalonyl-CoA: 214.6
  Methoxymalonyl-CoA: 172.9
  2-Methylbutyryl-CoA: 159.8
  trans-1,2-CPDA: 137.4
  Propionyl-CoA: 116.6
  Malonyl-CoA: 102.4
  CHC-CoA: 93.7
  Isobutyryl-CoA: 84.7
  Benzoyl-CoA: 71.5
  Acetyl-CoA: 59.2
  3-Methylbutyryl-CoA: 33.7
  fatty_acid: 29.9
  inactive: 25.3

apoS5: mal - mal - pk
PKS_AT (543..822): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 83.3%
  Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 194.4
  inactive: 96.9
  Methoxymalonyl-CoA: 59.1
  Methylmalonyl-CoA: 50.6
  Propionyl-CoA: 44.4
  Benzoyl-CoA: 22.6
  Isobutyryl-CoA: 17.3
  Acetyl-CoA: 13.7
  2-Methylbutyryl-CoA: 11.7
  trans-1,2-CPDA: 11.3
  fatty_acid: 10.2
  CHC-CoA: 8.9
  3-Methylbutyryl-CoA: 0.0
  Ethylmalonyl-CoA: -0.7

PKS_AT (1973..2264): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 87.5%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 216.7
  inactive: 121.6
  Methoxymalonyl-CoA: 73.4
  Methylmalonyl-CoA: 66.9
  Isobutyryl-CoA: 49.9
  Propionyl-CoA: 47.8
  Ethylmalonyl-CoA: 33.5
  2-Methylbutyryl-CoA: 32.9
  Benzoyl-CoA: 31.8
  Acetyl-CoA: 30.4
  CHC-CoA: 23.4
  fatty_acid: 18.8
  trans-1,2-CPDA: 14.7
  3-Methylbutyryl-CoA: 0.0

PKS_AT (4011..4301): pk
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 95.8%
  Methoxymalonyl-CoA: 91.7%
  Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:
  Ethylmalonyl-CoA: 227.0
  Methylmalonyl-CoA: 221.8
  Methoxymalonyl-CoA: 176.7
  2-Methylbutyryl-CoA: 160.9
  trans-1,2-CPDA: 142.5
  Propionyl-CoA: 118.3
  Malonyl-CoA: 111.2
  CHC-CoA: 97.4
  Isobutyryl-CoA: 87.9
  Benzoyl-CoA: 74.8
  Acetyl-CoA: 66.2
  fatty_acid: 35.7
  3-Methylbutyryl-CoA: 35.7
  inactive: 26.8

apoS4: mal
PKS_AT (77..354): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 75.0%
  Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 203.3
  inactive: 97.7
  Methoxymalonyl-CoA: 61.0
  Methylmalonyl-CoA: 55.7
  Propionyl-CoA: 41.6
  Ethylmalonyl-CoA: 25.7
  Benzoyl-CoA: 24.5
  Isobutyryl-CoA: 19.1
  Acetyl-CoA: 17.7
  2-Methylbutyryl-CoA: 15.5
  fatty_acid: 14.7
  trans-1,2-CPDA: 13.1
  CHC-CoA: 12.7
  3-Methylbutyryl-CoA: 0.0

apoS2: mal
PKS_AT (554..831): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 95.8%
  inactive: 75.0%
  Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 199.0
  inactive: 100.3
  Methoxymalonyl-CoA: 59.0
  Methylmalonyl-CoA: 55.7
  Propionyl-CoA: 39.7
  Ethylmalonyl-CoA: 28.7
  Benzoyl-CoA: 24.5
  Isobutyryl-CoA: 20.4
  2-Methylbutyryl-CoA: 18.8
  Acetyl-CoA: 17.9
  fatty_acid: 14.6
  trans-1,2-CPDA: 13.2
  CHC-CoA: 12.7
  3-Methylbutyryl-CoA: 0.0

apoS1: pk - mal - mmal - mmal
PKS_AT (549..845): pk
ATSignature: Methoxymalonyl-CoA

Top 3 matches:
  Methoxymalonyl-CoA: 95.8%
  Methylmalonyl-CoA: 91.7%
  Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:
  Ethylmalonyl-CoA: 192.8
  Methylmalonyl-CoA: 191.6
  Methoxymalonyl-CoA: 176.8
  Malonyl-CoA: 125.6
  2-Methylbutyryl-CoA: 124.5
  trans-1,2-CPDA: 115.9
  Propionyl-CoA: 112.4
  Isobutyryl-CoA: 94.7
  CHC-CoA: 91.1
  Acetyl-CoA: 65.4
  Benzoyl-CoA: 59.2
  3-Methylbutyryl-CoA: 53.2
  fatty_acid: 36.6
  inactive: 36.3

PKS_AT (2036..2330): mal
ATSignature: Malonyl-CoA

Top 3 matches:
  Malonyl-CoA: 91.7%
  inactive: 87.5%
  2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:
  Malonyl-CoA: 216.3
  inactive: 117.5
  Methoxymalonyl-CoA: 59.8
  Methylmalonyl-CoA: 56.9
  Isobutyryl-CoA: 27.7
  Propionyl-CoA: 26.2
  fatty_acid: 22.4
  Benzoyl-CoA: 20.0
  Ethylmalonyl-CoA: 15.6
  Acetyl-CoA: 12.4
  CHC-CoA: 5.2
  2-Methylbutyryl-CoA: 4.2
  3-Methylbutyryl-CoA: 2.9
  trans-1,2-CPDA: 0.0

PKS_AT (3724..4013): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 91.7%
  Methoxymalonyl-CoA: 83.3%
  Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 214.5
  Ethylmalonyl-CoA: 213.4
  Methoxymalonyl-CoA: 170.8
  2-Methylbutyryl-CoA: 159.8
  trans-1,2-CPDA: 137.5
  Propionyl-CoA: 122.3
  CHC-CoA: 104.3
  Malonyl-CoA: 104.0
  Isobutyryl-CoA: 85.7
  Benzoyl-CoA: 72.3
  Acetyl-CoA: 63.1
  fatty_acid: 40.9
  3-Methylbutyryl-CoA: 32.2
  inactive: 30.8

PKS_AT (5214..5511): mmal
ATSignature: Methylmalonyl-CoA

Top 3 matches:
  Methylmalonyl-CoA: 100.0%
  Methoxymalonyl-CoA: 95.8%
  Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:
  Methylmalonyl-CoA: 217.1
  Ethylmalonyl-CoA: 208.4
  Methoxymalonyl-CoA: 179.0
  2-Methylbutyryl-CoA: 168.3
  trans-1,2-CPDA: 126.8
  Propionyl-CoA: 117.9
  Malonyl-CoA: 116.4
  CHC-CoA: 97.9
  Isobutyryl-CoA: 91.7
  Benzoyl-CoA: 67.6
  Acetyl-CoA: 57.0
  inactive: 41.5
  3-Methylbutyryl-CoA: 39.4
  fatty_acid: 21.5

Link to PARAS substrate predictor