BGC0000023 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000023 (Stigmatella aurantiaca DW4/3-1)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	58,500	High	aurafuron A	PKS:Type I

BGC0000023 - Region 1 - T1PKS

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Location: 1 - 58,500 nt. (total: 58,500 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 58,501
Label		BGC0000023

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

CAO98842.1: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

aufA: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

aufB: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

aufH: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

aufJ: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: acylase

aufF: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

aufG: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Ligases: ligase synthase

CAO98843.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

aufC: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

aufD: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

aufE: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

aufI: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000023.5	1.00	PKS	aurafuron A	Stigmatella aurantiaca DW4/3-1
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.77	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.77	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001254.3	0.67	PKS	ACT-Toxin II	Alternaria alternata
BGC0000041.3	0.67	PKS	oronofacic acid	Pseudomonas syringae
BGC0001164.3	0.67	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001284.4	0.67	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001160.5	0.66	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000023.5	1.00	PKS	aurafuron A	Stigmatella aurantiaca DW4/3-1
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.77	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.77	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001254.3	0.67	PKS	ACT-Toxin II	Alternaria alternata
BGC0000041.3	0.67	PKS	oronofacic acid	Pseudomonas syringae
BGC0001164.3	0.67	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001284.4	0.67	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001160.5	0.66	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 58500:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (Me-ccmal - ohmal) + (ohmxmal) + (ccmal - Me-ohmal) + (Me-mal)
Non-colinear ordering:: aufC + aufD + aufE + aufF + aufG

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CAO98843.1: NH2

CAL_domain (76..514): NH2

minowa: NH2

Prediction, score:

NH2: 25.0
fatty_acid: 20.9
shikimic_acid: 10.5
AHBA: 3.8
Acetyl-CoA: -4.0

aufC: mmal

PKS_AT (567..860): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Methoxymalonyl-CoA: 79.2%; Ethylmalonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 140.8
Ethylmalonyl-CoA: 116.0
Methoxymalonyl-CoA: 109.3
Malonyl-CoA: 102.6
Propionyl-CoA: 77.2
Isobutyryl-CoA: 76.8
2-Methylbutyryl-CoA: 72.3
3-Methylbutyryl-CoA: 60.6
Benzoyl-CoA: 52.1
trans-1,2-CPDA: 51.0
CHC-CoA: 48.8
Acetyl-CoA: 42.5
inactive: 35.6
fatty_acid: 29.4

aufD: mmal - mal

PKS_AT (568..861): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 83.3%; Methoxymalonyl-CoA: 75.0%; Ethylmalonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 158.8
Ethylmalonyl-CoA: 142.7
Methoxymalonyl-CoA: 130.2
Isobutyryl-CoA: 101.9
Malonyl-CoA: 93.0
2-Methylbutyryl-CoA: 87.5
trans-1,2-CPDA: 85.8
Propionyl-CoA: 85.8
Benzoyl-CoA: 77.7
CHC-CoA: 69.5
Acetyl-CoA: 59.7
3-Methylbutyryl-CoA: 39.4
fatty_acid: 38.6
inactive: 24.2

PKS_AT (2377..2666): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 66.7%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 161.6
inactive: 94.2
Methylmalonyl-CoA: 88.5
Methoxymalonyl-CoA: 87.0
Propionyl-CoA: 61.2
Isobutyryl-CoA: 59.0
Ethylmalonyl-CoA: 50.5
2-Methylbutyryl-CoA: 42.4
Benzoyl-CoA: 32.4
CHC-CoA: 28.5
trans-1,2-CPDA: 24.5
Acetyl-CoA: 20.4
3-Methylbutyryl-CoA: 18.1
fatty_acid: 16.6

aufE: mxmal

PKS_AT (553..779): mxmal

ATSignature: (unknown)

No matches above 50%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 42.1
Isobutyryl-CoA: 34.6
Methylmalonyl-CoA: 31.1
Propionyl-CoA: 26.3
Malonyl-CoA: 22.9
2-Methylbutyryl-CoA: 21.0
Benzoyl-CoA: 17.6
Acetyl-CoA: 17.5
trans-1,2-CPDA: 17.2
CHC-CoA: 12.2
3-Methylbutyryl-CoA: 11.2
Ethylmalonyl-CoA: 3.9
inactive: 0.0
fatty_acid: 0.0

aufF: mal - mmal

PKS_AT (542..833): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 174.9
inactive: 124.6
Methoxymalonyl-CoA: 78.8
Methylmalonyl-CoA: 75.6
Ethylmalonyl-CoA: 57.4
Propionyl-CoA: 50.9
Isobutyryl-CoA: 45.4
Acetyl-CoA: 43.0
Benzoyl-CoA: 36.1
2-Methylbutyryl-CoA: 34.8
CHC-CoA: 28.5
fatty_acid: 26.4
trans-1,2-CPDA: 18.4
3-Methylbutyryl-CoA: 9.3

PKS_AT (2372..2665): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 75.0%; Malonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 159.2
Ethylmalonyl-CoA: 147.8
Methoxymalonyl-CoA: 125.1
2-Methylbutyryl-CoA: 91.4
Propionyl-CoA: 87.6
trans-1,2-CPDA: 78.9
Malonyl-CoA: 75.8
Isobutyryl-CoA: 72.6
Benzoyl-CoA: 63.8
CHC-CoA: 63.4
Acetyl-CoA: 52.9
fatty_acid: 44.2
3-Methylbutyryl-CoA: 36.0
inactive: 21.4

aufG: mmal

PKS_AT (571..864): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 83.3%; Methoxymalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 186.7
Ethylmalonyl-CoA: 171.1
Methoxymalonyl-CoA: 147.7
Isobutyryl-CoA: 112.6
2-Methylbutyryl-CoA: 110.3
Malonyl-CoA: 102.8
Benzoyl-CoA: 101.1
trans-1,2-CPDA: 98.8
CHC-CoA: 93.4
Propionyl-CoA: 85.2
Acetyl-CoA: 60.3
3-Methylbutyryl-CoA: 51.7
fatty_acid: 42.0
inactive: 24.7

aufI: mmal - mmal

PKS_AT (619..916): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 62.5%; Benzoyl-CoA: 58.3%; Methoxymalonyl-CoA: 54.2%

minowa: Benzoyl-CoA

Prediction, score:

Benzoyl-CoA: 133.3
Methylmalonyl-CoA: 110.5
Methoxymalonyl-CoA: 97.0
Isobutyryl-CoA: 95.2
Ethylmalonyl-CoA: 83.2
CHC-CoA: 50.8
2-Methylbutyryl-CoA: 47.9
Propionyl-CoA: 46.5
trans-1,2-CPDA: 45.8
Malonyl-CoA: 43.5
3-Methylbutyryl-CoA: 40.8
fatty_acid: 20.8
Acetyl-CoA: 15.6
inactive: 0.0

PKS_AT (1046..1343): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 208.7
Ethylmalonyl-CoA: 201.1
Methoxymalonyl-CoA: 177.6
trans-1,2-CPDA: 136.4
2-Methylbutyryl-CoA: 131.1
Malonyl-CoA: 102.7
CHC-CoA: 101.8
Propionyl-CoA: 95.8
Isobutyryl-CoA: 80.8
Benzoyl-CoA: 75.8
Acetyl-CoA: 52.1
3-Methylbutyryl-CoA: 48.7
inactive: 35.9
fatty_acid: 26.7

Link to PARAS substrate predictor