LOCUS       BGC0000025              9530 bp    DNA     linear   BCT 12-JUN-2009
DEFINITION  Streptomyces avermitilis avermectin biosynthetic gene cluster (orf1,
            aveBI, aveBII, aveBIII, aveBIV, aveBV, aveBVI, aveBVII, aveBVIII),
            complete cds.
ACCESSION   BGC0000025
VERSION     BGC0000025
KEYWORDS    .
SOURCE      Streptomyces avermitilis
  ORGANISM  Streptomyces avermitilis
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 14:44:48
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 9530
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..9530
                     /db_xref="taxon:33903"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces avermitilis"
     subregion       1..9530
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Polyketide"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..9530
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            160..873
                     /gene="orf-1"
     CDS             160..873
                     /codon_start=1
                     /gene="orf-1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     152.9; E-value: 1.8e-46)"
                     /gene_kind="biosynthetic-additional"
                     /note="contains NAD(P)H binding domain; not function in
                     avermectin biosynthesis"
                     /product="reductase"
                     /protein_id="BAA84591.1"
                     /transl_table=11
                     /translation="MCVDRDGRGAGVTADLARTRGARAAWPEVADVSDGAAMERFAERV
                     AETYGVVDLLVNNAGIGMAGRFLDTSVEDWQRTLGVNLWGVIHGCRLIGRQMAERGQGG
                     HIVTVASAAAFQPTRAVPAYATSKAAVLMLSECLRAEFAEFGVGVSVVCPGFVRTSFAS
                     AMHFAGVPRLEQERLRALFAGRGCSAEKVAAAVLRSVARDSAVVTVTAEARLSRLMSRF
                     TPRLRAAVARMDPPS"
     gene            931..2169
                     /gene="aveBI"
     CDS             931..2169
                     /codon_start=1
                     /gene="aveBI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MGT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1062:glycosyltransferase, MGT family (Score: 266.8;
                     E-value: 6.6e-81)"
                     /gene_kind="biosynthetic-additional"
                     /note="transfer of oleandrose onto polyketide-derived
                     aglycons"
                     /product="glycosyl transferase"
                     /protein_id="BAA84592.1"
                     /sec_met_domain="MGT (E-value: 2.1e-46, bitscore: 148.1,
                     seeds: 193, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDHFLFMSAPFWGHVFPSLAVAEELVHRGHHVTFVTGAEMADAV
                     RSVGADFLRYESAFEGVDMYRLMTEAEPNAIPMTLYDEGMSMLRSVEEHVGKDVPDLVA
                     YDIATSLNVGRVLAASWSRPAMTVIPLFASNGRFSTMQSVLDPDSAQVSAPPPRFSEQM
                     ELFGLGALVPRLAELLVSRGITEPVDDFLSGPEDFNLVCLPRAFQYAGDTFDERFAFVG
                     PCLGKRRGLGEWTPPGSGHPVVLISLGTVFNRQLSFFRTFVRAFTDVPVHVVISLGKGV
                     DPDVLRPLPPNVEVHRWVPHHAVLEHARALVTHGGTGSVMEALHAGCPVLVMPLSRDAQ
                     VTGRRIAELGLGRMVQPEEVTATTLRRHVLDIISDDAITRQVRQMQRATVEAGGALRAA
                     DETERFLRRTRRH"
     gene            complement(2075..3142)
                     /gene="aveBII"
     CDS             complement(2075..3142)
                     /NRPS_PKS="Domain: NAD_binding_4 (1-181). E-value: 1.3e-20.
                     Score: 65.4. Matches aSDomain:
                     nrpspksdomains_aveBII_NAD_binding_4.1"
                     /codon_start=1
                     /gene="aveBII"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     346.4; E-value: 2.6e-105)"
                     /gene_kind="biosynthetic-additional"
                     /note="function in oleandrose biosynthesis"
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="BAA84593.1"
                     /sec_met_domain="NAD_binding_4 (E-value: 2.2e-21, bitscore:
                     65.4, seeds: 102, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 2.3e-20,
                     bitscore: 62.1, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 6e-22, bitscore:
                     67.3, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGGAGFIGSHFVRRLLTGAYPAFTGAEVVVLDKLTYAGRLENLA
                     PVLGSPSLIFVHGDICDGPLVADLMDGSDMVVHFAAESHVDRSVADAAEFVRTNVLGTH
                     TLLRAATDAAVDRFVYISTDEVYGSIDSGSWTEDAPLEPNSPYSASKASSDLLARSFHR
                     THGLPVIITRCSNNYGPHQFPEKLIPRFVTHLLNGTKVPLYGDGENVRDWLHVDDHCRG
                     IALVAERGRPGEIYHIGGGTELSNRELTARLLDLLGVDWSMVEPVTDRKGHDRRYSLDI
                     SKISAELGYAPRVPFEEGLAQTVQWYVENRTLWEPLTARPELPVSDGASGAETARSRPL
                     PAGRRPPRPWPAASA"
     aSDomain        complement(2600..3139)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_aveBII_NAD_binding_4.1"
                     /evalue="1.30E-20"
                     /label="aveBII_NAD_binding_4.1"
                     /locus_tag="aveBII"
                     /protein_end="181"
                     /protein_start="1"
                     /score="65.4"
                     /tool="antismash"
                     /translation="TGGAGFIGSHFVRRLLTGAYPAFTGAEVVVLDKLTYAGRLENLAP
                     VLGSPSLIFVHGDICDGPLVADLMDGSDMVVHFAAESHVDRSVADAAEFVRTNVLGTHT
                     LLRAATDAAVDRFVYISTDEVYGSIDSGSWTEDAPLEPNSPYSASKASSDLLARSFHRT
                     HGLPVIITRCSNNYGPH"
     gene            complement(3139..4038)
                     /gene="aveBIII"
     CDS             complement(3139..4038)
                     /codon_start=1
                     /gene="aveBIII"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 287; E-value:
                     2.6e-87)"
                     /gene_kind="biosynthetic-additional"
                     /note="function in oleandrose biosynthesis"
                     /product="glucose-1-phosphate thymidyltransferase"
                     /protein_id="BAA84594.1"
                     /transl_table=11
                     /translation="MKGIVLAGGTGSRLYPLTRALSKQLLPVYDKPMIYYPLSVLMLGG
                     IKDILVISSPDHLEQFRRLLGDGSRLGLNIDYAAQQRPGGIAEAFLIGADFIGQDQVSL
                     VLGDNIFHGMGFSHLLRSHTRDVDGCVLFGYAVTDPERYGVGEVDASGKLLSVEEKPTA
                     PRSNLAITGLYLYDNDVIEVARGIRSSARGELEITDVNRAYLAEGRARLVDLGRGFTWL
                     DAGTHDSLMHAGQYVQVLEKRQGVRIACLEEIAFRMGLIDADDCYLRGVELAGSGYGEY
                     LMSIAAEAAVRSPGCAYS"
     gene            4164..5195
                     /gene="aveBIV"
     CDS             4164..5195
                     /codon_start=1
                     /gene="aveBIV"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score: 165;
                     E-value: 4.4e-50)"
                     /gene_kind="biosynthetic-additional"
                     /note="function in oleandrose biosynthesis"
                     /product="dTDP-4-keto-6-deoxy-L-hexose 4-reductase"
                     /protein_id="BAA84595.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.8e-13, bitscore:
                     38.8, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGRFSVCPPRPTGILKSMLTTGMCDRPLVVVLGASGYIGSAVAAE
                     LARWPVLLRLVARRPGVVPPGGAAETETRTADLTAASEVALAVTDADVVIHLVARLTQG
                     AAWRAAESDPVAERVNVGVMHDVVAALRSGRRAGPPPVVVFAGSVYQVGRPGRVDGSEP
                     DEPVTAYARQKLDAERTLKSATVEGVLRGISLRLPTVYGAGPGPQGNGVVQAMVLRALA
                     DEALTVWNGSVVERDLVHVEDVAQAFVSCLAHADALAGRHWLLGSGRPVTVPHLFGAIA
                     AGVSARTGRPAVPVTAVDPPAMATAADFHGTVVDSSAFRAVTGWRPRLSLQEGLDHMVA
                     AYV"
     gene            complement(5249..5926)
                     /gene="aveBV"
     CDS             complement(5249..5926)
                     /codon_start=1
                     /gene="aveBV"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) dTDP_sugar_isom"
                     /gene_kind="biosynthetic-additional"
                     /note="function in oleandrose biosynthesis"
                     /product="dTDP-4-keto-6-deoxyhexose 3,5-epimerase"
                     /protein_id="BAA84596.1"
                     /sec_met_domain="dTDP_sugar_isom (E-value: 1.9e-59,
                     bitscore: 189.4, seeds: 19, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLGRPRRSSADRPAPPAGARATAAGVTVRRLVVEGAVEFTPTVF
                     PDERGLFVTPYQEPVLSEAVGHRFPTAQTCQSVSRRGVVRGVHFTATPPGQAKYVHCAR
                     GRALDFVVDLRTGSPTFGQWDSVLLDQERFRSVYLPIGVGHAFVALEDDTAMVYLMSSG
                     YVPQNEHALSPEDPDLALPLGHHLGRAPILSERDRRAPTLQQALRRGMLPEYRASRGLD
                     EKL"
     gene            complement(5988..7397)
                     /gene="aveBVI"
     CDS             complement(5988..7397)
                     /codon_start=1
                     /gene="aveBVI"
                     /note="function in oleandrose biosynthesis"
                     /product="dTDP-4-keto-6-deoxy-L-hexose2,3-dehydratase"
                     /protein_id="BAA84597.1"
                     /transl_table=11
                     /translation="MSVRADADHTEPSTAHRAARRRPARVPRPLRRRGRHRRRTSLDAF
                     TGWWTRRSGAHRFRVERIPFHGMDAWSFHPGTGNLAHRSGRFFSVEGLHVRGGEQPFPE
                     WQQPIIHQPEIGILGILAKKFDGVLHFLMQAKMEPGNINLVQLSPTVQATRSNYTKVHG
                     GAAVKYLEYFTQPRRATVVVDVLQSEHGAWFHRKFNRNIVVETDEDVPLDDDFRWLTLG
                     QIGELMHRDNLVNMDARTVLACLPTPFDEPAALHSDAELLSWYAAERSRHSVHARRVPL
                     AGIPGWTTGAESIAHHADRYFRVVAVRVEASNREVAAWTQPLIEPCGHGITAFLTRRIG
                     GVPHLLAHGRVEGGFLDTIELGPTVQYTPRNYAHLTGPARPRFLDLVLEAAPDRIRYAA
                     VHSEEGGRFLHAQARYLFVEADESQAPNDPPPGYRWCTPGQLTQLLRYGRYVNVQARTL
                     LSLLTTRAVEL"
     gene            complement(7394..8167)
                     /gene="aveBVII"
     CDS             complement(7394..8167)
                     /codon_start=1
                     /gene="aveBVII"
                     /note="function in oleandrose biosynthesis"
                     /product="dTDP-6-deoxy-L-hexose 3-O-methyltransferase"
                     /protein_id="BAA84598.1"
                     /transl_table=11
                     /translation="MTEREFTDPRIVPHESEQERAAREQLTKLLVDSPIPPKYLIDNLS
                     VYMRRNQLADLLSMDALYRMLPEVPGVIMEFGVLHGRHLATLTALRSIYEPYNSLRRVI
                     GFDTFTGFPDIDEADEVSTSAVPGRFAVPDGEVEHLRQVLAAHEANEPYGHTQRSFVVQ
                     GDVRETVPQYLAEHPHTVIALAYFDLDLYRPTRELLDVITPHLTRGSILAFDELTHPKW
                     PGETRALSEAFGLDHAPLRQLPGREPPVIYMTWGA"
     gene            complement(8254..9297)
                     /gene="aveBVIII"
     CDS             complement(8254..9297)
                     /codon_start=1
                     /gene="aveBVIII"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039:aldo/keto reductase family oxidoreductase (Score:
                     312.2; E-value: 5.7e-95)"
                     /gene_kind="biosynthetic-additional"
                     /note="function in oleandrose biosynthesis"
                     /product="dTDP-4-keto-6-deoxy-L-hexose 2,3-reductase"
                     /protein_id="BAA84599.1"
                     /transl_table=11
                     /translation="MMPTTAEPAPVQSDSNSSAPLHTELGRTRLRISRLALGTVNIGGR
                     VEEPEARRLMDHALAQGITLFDTANTYGWRVHKGYTEEVIGRWLADRPARREQVVLATK
                     VGDPMGSGPNDHGLSVRNIVAACDASLRRLRTDWIDLYQLHHIDRRAGWDEVWQAMDLL
                     ITQGKVRYVGSSNFAGWDIASAQEAARRRNALGLASEQCVYNLVTRHAELEVIPAASAY
                     GVGVLVWSPLHGGLLGGVLRKTRENTAVKSAQGRAVEALEHHRTTIAAYEDVCADHGLD
                     PAHVGMAWVLSRPGVTGLVIGPRTEQHVDGALHALRTPLPEPVLARLEELFPPVGRGGS
                     APDAWLS"
ORIGIN
        1 cagacccctg atgctcgatg aatgccgcta tagctagggg gtatggcgcc agacatgaat
       61 tcacagcgtt tcggcggccg gctggcgctt gtcacaggtg caggcggtgg catcgggcgg
      121 gcgaccgcct gcgctctcgg atcggccggg gcgcgagtgg tgtgcgtgga ccgggacggc
      181 cgcggcgccg gggtgacggc cgacctggcc cggacgcggg gcgcgcgggc ggcctggccc
      241 gaggtggccg acgtgtccga cggagcggcg atggagcggt tcgccgagcg cgtcgccgag
      301 acgtacgggg tcgtggacct gctggtgaac aacgccggca tcggcatggc ggggcgtttt
      361 ctcgacacgt ccgtcgagga ctggcagcgc accctgggcg tcaacctctg gggtgtcatt
      421 catggttgcc gcctcatcgg ccggcagatg gcggagcgcg ggcagggcgg gcacatcgtg
      481 acggtggcgt cggcggcggc gttccagccg acgcgggcgg tccccgcgta tgccaccagc
      541 aaggcggcgg tgctgatgct gagcgagtgc ctgcgcgcgg agttcgcgga gttcggggtc
      601 ggagtgagcg tggtgtgccc gggcttcgtc cgtacgtcgt tcgcgtcggc gatgcatttc
      661 gccggtgtgc cccggctgga gcaggagcgg ctgcgggcgc tgttcgccgg tcgcggatgc
      721 agcgcggaga aggtggccgc ggcggtactg cggtcggtgg cgcgcgactc ggccgtggtg
      781 accgtgacgg cggaagcgcg gctgtcacgg ctgatgagcc gcttcacgcc acgcctgcgc
      841 gccgcggtgg cgcggatgga tcccccttcg tagggctggc ggggatcccc tccttgcctt
      901 cgaacatctt ccgacgatgg gcagtgagag atgtcagatc attttctctt catgagtgcg
      961 ccgttctggg ggcatgtgtt ccccagtctc gccgtggcgg aggagctcgt gcaccggggc
     1021 caccacgtca cctttgtgac gggcgcggaa atggccgatg cggtgcgttc cgtgggcgct
     1081 gatttcctgc ggtacgagtc cgccttcgag ggtgtcgaca tgtaccggct gatgaccgag
     1141 gccgagccga acgccatccc catgacgctg tacgacgagg gcatgtccat gttgcgttcg
     1201 gtggaggagc acgtcggcaa ggacgttccg gacctggtgg cctacgacat cgccacctcc
     1261 ctcaacgtgg gtcgtgtcct cgccgcctcc tggagcaggc cggccatgac ggtcattccc
     1321 ctgttcgcgt ccaacgggcg cttctccacg atgcagtcgg tattggatcc ggattccgct
     1381 caggtcagtg cgccgccgcc gcgcttctcg gagcagatgg agttgttcgg cctcggggcg
     1441 ctggtgccgc gcctcgcgga gctgctcgtt tcccggggta tcacggaacc ggtcgacgat
     1501 ttcctttccg gaccggagga cttcaacctg gtgtgtctgc cgcgcgcctt ccagtacgcg
     1561 ggcgacacct tcgacgagcg gttcgccttc gtcggaccat gtctgggtaa gcgcaggggt
     1621 ctgggcgagt ggacaccacc gggcagcggg catccagtgg tgctcatctc cctcgggacc
     1681 gtgttcaacc ggcagctgtc cttcttccgc acgttcgtcc gggcgttcac cgacgtcccc
     1741 gtgcacgtcg tgatctcgct cggcaagggg gtcgaccccg atgtgctgcg gccgctgccg
     1801 ccgaatgtcg aggtgcaccg gtgggtgccg caccatgcgg tgctggagca tgccagggct
     1861 ctggtcacgc acggcggtac cggcagtgtg atggaggcac tgcacgcagg gtgcccggtg
     1921 ctcgtcatgc ccttgtcgcg ggacgcgcag gtgaccggcc ggcggatcgc cgagctgggg
     1981 ctgggtcgta tggtgcagcc ggaggaggtc acggcgacga cgctgcgccg gcacgtgctg
     2041 gacatcatct ccgatgacgc gatcacccga caggtcaggc agatgcagcg ggccacggtc
     2101 gaggcgggcg gcgccctgcg ggcagcggac gagaccgagc ggtttctgcg ccggacgcgc
     2161 cgtcactgac cggcagctcg ggccgggcgg tgagtggctc ccacagggtt cggttctcca
     2221 cgtaccactg aacggtctgt gccagcccct cctcgaaggg cacgcggggc gcgtaaccga
     2281 gctcggcgga gatcttgctg atgtccagcg agtagcgccg gtcgtgcccc ttgcggtcgg
     2341 tcacgggttc gaccatcgac cagtccacgc cgagcaggtc caggagccgg gcggtgagct
     2401 cacggttgga cagctccgtc ccgcctccga tgtggtagat ctcgccgggc ctgccgcgtt
     2461 cggcgaccag ggcgatgcca cggcagtggt cgtccacgtg cagccagtcg cggacgtttt
     2521 cgccgtcgcc gtacaagggc accttcgtgc cgttcagcag atgggtgacg aaccgcggga
     2581 tgagtttctc cgggaactgg tgggggccgt agttgttcga gcatcgggtg atgatcactg
     2641 gtaggccgtg cgtgcggtgg aaggaccggg cgagcaggtc ggaggacgcc ttggacgcgg
     2701 agtagggcga gttcggctcc agcggggcgt cctcggtcca cgagccggag tcgatggagc
     2761 cgtagacctc gtccgtcgag atgtacacga agcggtccac ggcggcgtcg gtggcggcgc
     2821 ggagcagggt gtgagtgccg aggacattgg tgcgtacgaa ctcggcggcg tcggccacgg
     2881 accggtccac gtgtgactcc gccgcgaagt ggaccaccat gtcggagccg tccatcaggt
     2941 ccgcgaccaa gggcccgtcg cagatgtcgc cgtgcacgaa gatcagggat gggcttccca
     3001 ggaccggtgc gaggttctcc aggcgacccg cgtaggtcag cttgtcgagc accacgacct
     3061 cggcaccggt gaacgccgga tacgcgcccg tcagcaaccg ccgtacgaaa tgggaaccga
     3121 tgaaaccggc gccgcccgtc acgagtaggc gcatcccggg ctcctcaccg cggcttccgc
     3181 cgcaatactc atcagatact cgccgtagcc ggagccggcc agttcgaccc cgcgcagata
     3241 gcagtcgtcc gcgtcgatca gacccatccg gaaggcgatc tcctcgagac aggcgatccg
     3301 tactccctgg cgcttctcca ggacctgcac atactgcccg gcgtgcatca gcgagtcgtg
     3361 cgtccccgca tcgagccagg tgaagccccg gcccaggtcc accagccggg cccgcccctc
     3421 ggcgaggtag gccctgttga cgtcggtgat ctccagctcg ccgcgggccg acgagcggat
     3481 gccccgggcc acctcgatca cgtcgttgtc gtacaggtac aggcctgtga tcgccaggtt
     3541 ggaccggggg gcggtgggtt tctcctcgac ggacagcagc tttccggagg cgtcgacctc
     3601 tccgactccg taccgttcgg gatccgtcac cgcgtatccg aacaacacac agccgtcgac
     3661 atcgcgggtg tggctgcgca gcaggtgcga aaagcccatg ccatggaaga tgttgtcccc
     3721 aaggacaagg gacacctgat cctgaccgat gaaatcggcg ccgatgagga atgcctcggc
     3781 gattcctccc ggtcgctgct gcgcggcgta gtcgatgttc agcccgaggc ggcttccgtc
     3841 tccgagcagt ctccggaatt gttcgagatg atcgggtgag gaaatcacca ggatgtcttt
     3901 tatgccgccg agcatcaaca cggagagcgg gtagtagatc atgggtttgt cgtagacagg
     3961 gagcagctgc ttggaaaggg cacgggtcaa cgggtaaagc cgagagccgg ttccccccgc
     4021 gagcacgatt cccttcatgt cggactcccc gcagtcgacg ttatatatct ctgccgtctg
     4081 cccgacggta ccaagtggcg gaaaacgcac caggaattcg agcgccgcta gggggaaggg
     4141 ctcaagaaga taggggccac cagatggggc ggttttcggt gtgcccgccc cggccgaccg
     4201 gaatactgaa gagcatgctg acgactggga tgtgcgaccg accgctggtc gtcgtactcg
     4261 gagcctccgg ctatatcggg tcggccgtcg cggcggaact cgcccggtgg ccggtcctgt
     4321 tgcggctggt ggcccggcga ccgggcgtcg ttccgccggg cggcgccgcg gagaccgaga
     4381 cgcgtacggc cgacctgacg gcggcgagcg aggtcgccct cgccgtgacg gacgccgacg
     4441 tggtgatcca cctggtcgcg cgcctcaccc agggagcggc atggcgggcg gcggagagcg
     4501 atccggtggc cgagcgggtg aacgtcgggg tgatgcacga cgtcgtcgcg gccctgcggt
     4561 ccgggcgccg cgccgggccg cccccggtgg tggtgttcgc cgggtcggtc taccaggtgg
     4621 gccgcccggg tcgggtcgac ggcagtgagc cggacgagcc cgtgacggcc tatgcccgtc
     4681 agaaactcga cgccgaacgg acgttgaagt ccgccacggt cgagggtgtc ctgcggggga
     4741 tctcgctgcg gctgcccacc gtctacggcg cggggccggg cccgcagggc aacggcgtcg
     4801 tgcaggcgat ggtgctccgg gcgctcgccg acgaggccct caccgtgtgg aacggaagcg
     4861 tggtggagcg tgacctggtg catgtggagg atgtcgcgca ggccttcgtg agctgcctgg
     4921 cgcacgcgga tgcgctcgcc gggcggcact ggctgctcgg cagcggtcgt cctgtgaccg
     4981 tcccgcacct cttcggtgcc atcgccgccg gcgtgtccgc ccgcaccggg cgccccgcgg
     5041 tgcccgtgac cgcggtggac cctccggcga tggcgacggc ggcggacttc cacgggaccg
     5101 tcgtcgactc ctcggcgttc cgcgcggtca ccgggtggcg gccgcggctg tcgcttcagg
     5161 agggcctgga ccacatggtg gcggcttacg tgtagcgccg gggtggcggc cgggcccggg
     5221 cggtgacggc ccggatccgg gtcggccgtc acagcttctc gtcgaggccg cggctcgcgc
     5281 ggtactccgg caacatgccg cgtcgcaggg cctgctggag agtcggcgcg cgccggtcgc
     5341 gctcggagag gatcggtgcc cgcccgaggt ggtggccgag gggcagggcg aggtccggat
     5401 cctcgggcga gagggcgtgt tcgttctgcg gaacgtagcc gctcgacatc aggtacacca
     5461 tcgccgtgtc gtcttccagc gccacgaacg cgtgcccgac cccgatcggc aggtagacgg
     5521 aacggaagcg ctcctggtcg aggaggaccg agtcccactg cccgaaagtc ggtgagccgg
     5581 tgcgcaggtc gacgacgaag tccagggccc gtccccgggc gcagtggacg tacttggcct
     5641 ggccgggtgg tgtcgcggtg aagtgcacgc cgcggacgac gccgcggcgc gagacgctct
     5701 ggcaggtctg cgcggtggga aaccggtgcc cgacggcctc gctgaggacc ggttcctggt
     5761 agggggtgac gaagagcccg cgctcgtcgg ggaagaccgt cggggtgaat tcgacggcgc
     5821 cctcgacgac gagcctccgg accgtgacac cggcggcggt ggcccgggcg cccgcgggcg
     5881 gggcgggccg gtcggcggag ctccggcgag gccggccaag ggtcatcgct gcactctctc
     5941 tgtcgtgcgg gttgtcatac gggtagtcgt acgggccggt tccggagtca cagctcgacg
     6001 gcgcgggtgg tgagcaggga cagcagggtg cgggcctgca cgttcacgta acggccgtac
     6061 cgcagcagct gggtcagctg gcccggggtg caccagcggt accccggggg cgggtcgttc
     6121 ggcgcctggc tctcgtcggc ctcgacgaac aggtagcgcg cctgtgcgtg cagaaagcga
     6181 ccgccctcct ccgagtggac cgccgcgtag cggatgcggt cgggcgcggc ctccagcacc
     6241 aggtcgagga agcgcggcct ggccggtccc gtgaggtggg cgtagttgcg cggggtgtac
     6301 tggaccgtcg ggccgagttc gatcgtgtcg aggaagccgc cctcgaccct gccgtgggcg
     6361 agcaggtgcg gtacgccgcc gatccgccgg gtcaggaagg cggtgatgcc gtggccgcac
     6421 ggttcgatca ggggctgggt ccaggcggcg acctcccggt tggaggcctc gacacggacc
     6481 gcgaccacac ggaagtaccg gtccgcgtgg tgggcgatgg actccgcgcc cgtggtccag
     6541 ccggggatgc cggccagggg cacgcggcgg gcgtgcacgg agtgccggga gcgttcggcg
     6601 gcgtaccagg agagcagttc ggcgtcgctg tgcagggccg cgggctcgtc gaacggggtg
     6661 ggaaggcagg cgaggaccgt gcgtgcgtcc atgttcacca ggttgtcccg gtgcatcagt
     6721 tcgccgatct gccccagtgt cagccagcgg aagtcgtcgt ccagtggtac gtcctcgtcg
     6781 gtctccacca cgatgttgcg gttgaacttc cggtggaacc aggctccgtg ctcggactgg
     6841 aggacgtcga ccaccacggt ggcgcgccgg ggctgtgtga agtactcgag gtacttcacg
     6901 gcggcgcccc cgtggacctt ggtgtagttg ctgcgcgtgg cctgcacggt gggcgacagc
     6961 tggaccaggt tgatgttgcc gggctccatc ttggcctgca tcaggaagtg caggaccccg
     7021 tcgaacttct tggcgaggat gccgaggatg ccgatctcgg gctggtggat gatgggctgc
     7081 tgccattccg ggaagggctg ttcaccgcct cggacgtgca gtccctccac ggagaagaac
     7141 cggccgctgc ggtgggccag attgccggtt ccggggtgaa acgaccaggc gtccatcccg
     7201 tggaagggga tgcgctcgac ccggaaccgg tgggccccgg accgccgcgt ccaccagccg
     7261 gtgaacgcgt cgagggacgt ccggcgccgg tgtcgcccac ggcggcggag cgggcgaggc
     7321 acgcgggcag ggcggcgtcg tgccgcgcgg tgagcggtgc tgggctcggt gtggtcggca
     7381 tcggctcgta cgctcatgca ccccacgtca tgtagatcac cggtggctcg cggccgggca
     7441 gttggcgcag tggggcgtgg tcgaggccga acgcctcgct cagcgccctg gtctcccccg
     7501 gccatttggg gtgggtgagt tcgtcgaagg cgaggatgct gcccctggtc aggtgcggtg
     7561 tgatgacgtc cagcagttcg cgcgtggggc ggtagaggtc caggtcgaag taggccagcg
     7621 cgatgacggt gtgcgggtgt tccgccaggt attggggcac cgtttcgcgt acgtcgccct
     7681 ggaccacgaa ggaacgctgg gtgtggccgt agggttcgtt cgcctcgtgc gccgcgagca
     7741 cctgccgcag gtgctccact tcgccgtccg gcacggcgaa ccgcccaggg accgcgctgg
     7801 tgctgacctc gtccgcctcg tcgatgtcgg ggaagccggt gaacgtgtcg aagccgatga
     7861 cgcggcgcag cgagttgtac ggctcataga tgctgcgcag cgcggtcagc gtggcgaggt
     7921 gccgtccgtg cagaacgccg aactccatga tgacgccggg gacttccggc agcatgcggt
     7981 acagcgcgtc catggagagc aggtcggcga gctggttgcg ccgcatgtag acggacaggt
     8041 tgtcgatcag gtacttcggc gggatcgggc tgtcgacgag gagcttggtc agctgctcgc
     8101 gggcagcgcg ttcctgctcg gactcgtgcg gcacgatccg gggatcggtg aactcccgct
     8161 cggtcatgga ggcctttcct ttcatgggtc ggtaccgggc gcgccggacg tgccggtcgt
     8221 accgggcgtg ccggcgggca cgacgctgtc gggtcaggac agccaggcgt cgggggcgga
     8281 tccgccgcgg ccgaccgggg ggaacagctc ctccaggcgg gccaggacgg gctcgggcag
     8341 cggggtgcgc agggcgtgca gtgccccgtc cacgtgctgt tcggtgcgcg gcccgatgac
     8401 cagcccggtc acgccgggcc gcgacagcac ccaggccatg ccgacatggg cggggtcgag
     8461 gccgtggtcc gcgcacacgt cctcgtacgc cgcgatggtg gtgcggtggt gctccagggc
     8521 ctcgacggcc cggccctgtg ccgacttgac cgcggtgttc tcccgcgtct tgcgcaggac
     8581 accgccgagc aggccgccgt gcagtggcga ccagaccagg acgccgacac cgtaggcgga
     8641 cgcggcgggg atgacttcca gctcggcgtg tcgggtcacg aggttgtaga cgcactgctc
     8701 ggaggcgagg cccagggcgt tgcgccgccg ggccgcctcc tgggcggaag cgatgtccca
     8761 gcccgcgaag ttggaggagc cgacgtagcg caccttgccc tgcgtgatga gcaggtccat
     8821 cgcctgccac acctcgtccc agccggcgcg gcggtcgatg tggtgcagct ggtacaggtc
     8881 gatccagtcg gtgcgcagtc ggcgcagcga ggcgtcgcag gcggccacga tattgcgtac
     8941 ggacagtccg tgatcgttgg ggccgctgcc catcggatcg ccgaccttgg tggccagcac
     9001 cacctgctca cgccgggcgg ggcggtccgc cagccacctg ccgatgacct cttcggtgta
     9061 ccccttgtgg acgcgccagc cgtaggtgtt ggcggtgtcg aacagggtga tgccctgagc
     9121 cagggcgtga tccatcagtc ggcgcgcttc gggctcctcc acccgtccgc cgatgttgac
     9181 cgttccgagc gccagtcggc tgatcctcag ccgggtcctg cccagttcgg tgtggagggg
     9241 agcactgctg ttgctgtcgg actggacggg tgcgggctcg gccgtcgtag gcatcatcga
     9301 tcagtcgaca ctccctcgtg cgtgagcggc gggcgctcga gcaggaccct gacctgaggc
     9361 ccaggaggct accggcgatc atgcgataca ggcagccgct cgatggtggg acacgggctg
     9421 ccgtcgccgg gcataggggc tgatgggggt tgtccggtgc gggtccggct gacagcttcg
     9481 tggacaccaa gttgatccag ttgatccact ccgaaaggca gaggctgcag
//
