Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000026.1:
Cluster 1Saccharide138410

BGC0000026, avilamycin A biosynthetic gene cluster from Streptomyces viridochromogenes. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Avilamycin A
PubChem ID: 122779
ChemSpider ID: 16735687
SMILES string: Copy to clipboard
Molecular formula: C61H88Cl2O32
Average molecular mass: 1404.243 Da
Molecular activity: Antibacterial
Molecular target: 50S ribosomal subunit

Class-specific details

Biosynthetic class(es):
Saccharide
Saccharide subclass:
oligosaccharide
Glycosyltransferases:
AAK83182.1:
Specificity: None
Evidence for specificity: Unknown
AAK83170.1:
Specificity: None
Evidence for specificity: Unknown
AAK83192.1:
Specificity: None
Evidence for specificity: Unknown
AAK83193.1:
Specificity: eurekanate
Evidence for specificity: Knock-out construct

Gene cluster description

avilamycin A (BGC0000026). Gene Cluster 1. Biosynthetic class = Saccharide. GenBank AF333038, positions 0-39999. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?incomplete
Evidence for cluster-compound connection:Knock-out studies
MIxS-compliance:Unknown
Contact for this cluster:Andreas Bechthold (Albert-Ludwigs-University of Freiburg, Germany)

Literature references

1. Weitnauer G et al. (2001) Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics. Chem Biol 8(6):569-81.
2. Weitnauer G et al. (2001) An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes Tu57. Antimicrob Agents Chemother 45(3):690-5. doi:
3. Weitnauer G et al. (2002) Analysis of a C-methyltransferase gene (aviG1) involved in avilamycin biosynthesis in Streptomyces viridochromogenes Tu57 and complementation of a Saccharopolyspora erythraea eryBIII mutant by aviG1. Microbiology 148(Pt 2):373-9. doi: 10.1099/00221287-148-2-373.
4. Weitnauer G et al. (2004) Novel avilamycin derivatives with improved polarity generated by targeted gene disruption. Chem Biol 11(10):1403-11. doi: 10.1016/j.chembiol.2004.08.016.
5. Treede I et al. (2005) Genes involved in formation and attachment of a two-carbon chain as a component of eurekanate, a branched-chain sugar moiety of avilamycin A. Appl Environ Microbiol 71(1):400-6. doi: 10.1128/AEM.71.1.400-406.2005.
6. Hofmann C et al. (2005) Genes encoding enzymes responsible for biosynthesis of L-lyxose and attachment of eurekanate during avilamycin biosynthesis. Chem Biol 12(10):1137-43. doi: 10.1016/j.chembiol.2005.08.016.
7. Boll R et al. (2006) The active conformation of avilamycin A is conferred by AviX12, a radical AdoMet enzyme. J Biol Chem 281(21):14756-63. doi: 10.1074/jbc.M601508200. Epub 2006
8. Martinez-Fleites C et al. (2006) Insights into the synthesis of lipopolysaccharide and antibiotics through the structures of two retaining glycosyltransferases from family GT4. Chem Biol 13(11):1143-52. doi: 10.1016/j.chembiol.2006.09.005.
9. Rebets Y et al. (2009) Production of avilamycin A is regulated by AviC1 and AviC2, two transcriptional activators. J Antibiot (Tokyo) 62(8):461-4. doi: 10.1038/ja.2009.63. Epub 2009 Jul
10. Mosbacher TG et al. (2005) Structure and function of the antibiotic resistance-mediating methyltransferase AviRb from Streptomyces viridochromogenes. J Mol Biol 345(3):535-45. doi: 10.1016/j.jmb.2004.10.051.
11. Treede I et al. (2003) The avilamycin resistance determinants AviRa and AviRb methylate 23S rRNA at the guanosine 2535 base and the uridine 2479 ribose. Mol Microbiol 49(2):309-18.
12. McNicholas PM et al. (2000) Evernimicin binds exclusively to the 50S ribosomal subunit and inhibits translation in cell-free systems derived from both gram-positive and gram-negative bacteria. Antimicrob Agents Chemother 44(5):1121-6.
13. Ninet L et al. (1974) Flambamycin, a new antibiotic from Streptomyces hygroscopicus DS 23 230. Experientia 30(11):1270-2.