BGC0000028 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000028 (Streptomyces lohii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	74,009	High	bafilomycin B1	PKS:Type I

BGC0000028 - Region 1 - T1PKS

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Location: 1 - 74,009 nt. (total: 74,009 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 74,010
Label		BGC0000028

Tailoring functions

Oxidoreductases: dehydrogenase

ADC79621.1: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

ADC79623.1: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: acylase

ADC79616.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

ADC79629.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase phosphatase

ADC79624.1: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

ADC79627.1: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

ADC79613.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

ADC79614.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

ADC79615.1: 8-amino-7-oxononanoate synthase

smcogs: SMCOG1109: 8-amino-7-oxononanoate synthase

ADC79617.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ADC79618.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ADC79619.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ADC79620.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000028.5	0.99	PKS	bafilomycin B1	Streptomyces lohii
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001348.5	0.70	PKS	JBIR-100	Streptomyces varsoviensis
BGC0000041.3	0.68	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000028.5	0.99	PKS	bafilomycin B1	Streptomyces lohii
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001348.5	0.70	PKS	JBIR-100	Streptomyces varsoviensis
BGC0000041.3	0.68	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 74009:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ohmal - ohmal - Me-mal) + (Me-ohmal - ohemal - ccmal) + (pk - Me-redmal) + (Me-ohmal - Me-ccmal) + (Me-ccmal)
Non-colinear ordering:: ADC79616.1 + ADC79617.1 + ADC79618.1 + ADC79619.1 + ADC79620.1

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ADC79613.1: X

AMP-binding (10..414): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TFCMFGGPVK
Nearest Stachelhaus code(s):: nLev GFCMYGGCSK (74% 8Å match)
Stachelhaus code match:: 60% (weak)

ADC79614.1: X

AMP-binding (15..418): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SSTLTVVFVK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

ADC79616.1: mmal - mmal - mal - mmal

PKS_AT (106..405): mmal

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 79.2%; Methoxymalonyl-CoA: 70.8%; Methylmalonyl-CoA: 70.8%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 161.1
Ethylmalonyl-CoA: 157.2
2-Methylbutyryl-CoA: 153.9
Methoxymalonyl-CoA: 134.5
Propionyl-CoA: 134.0
CHC-CoA: 94.5
trans-1,2-CPDA: 87.3
Malonyl-CoA: 84.7
Isobutyryl-CoA: 80.4
Benzoyl-CoA: 46.1
Acetyl-CoA: 34.9
3-Methylbutyryl-CoA: 30.8
inactive: 23.0
fatty_acid: 22.1

PKS_AT (1120..1414): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 211.9
Ethylmalonyl-CoA: 206.7
Methoxymalonyl-CoA: 168.8
2-Methylbutyryl-CoA: 162.1
trans-1,2-CPDA: 135.7
Propionyl-CoA: 119.4
CHC-CoA: 99.3
Malonyl-CoA: 87.5
Benzoyl-CoA: 81.7
Isobutyryl-CoA: 73.9
Acetyl-CoA: 55.4
fatty_acid: 41.1
3-Methylbutyryl-CoA: 32.0
inactive: 20.8

PKS_AT (2714..3015): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 235.9
inactive: 116.4
Methoxymalonyl-CoA: 72.9
Methylmalonyl-CoA: 60.1
Propionyl-CoA: 45.7
Isobutyryl-CoA: 36.7
Benzoyl-CoA: 21.5
Ethylmalonyl-CoA: 21.3
2-Methylbutyryl-CoA: 17.5
Acetyl-CoA: 17.1
CHC-CoA: 12.0
fatty_acid: 9.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (4311..4607): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 201.0
Ethylmalonyl-CoA: 198.4
Methoxymalonyl-CoA: 165.9
2-Methylbutyryl-CoA: 142.8
trans-1,2-CPDA: 122.0
Propionyl-CoA: 113.3
CHC-CoA: 91.3
Malonyl-CoA: 81.6
Isobutyryl-CoA: 75.6
Benzoyl-CoA: 69.2
Acetyl-CoA: 47.8
3-Methylbutyryl-CoA: 42.0
fatty_acid: 37.7
inactive: 29.7

ADC79617.1: mmal - emal - mal

PKS_AT (578..878): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.6
Ethylmalonyl-CoA: 205.1
Methoxymalonyl-CoA: 174.1
2-Methylbutyryl-CoA: 165.4
trans-1,2-CPDA: 119.5
Propionyl-CoA: 115.7
CHC-CoA: 106.6
Malonyl-CoA: 93.4
Isobutyryl-CoA: 80.9
Benzoyl-CoA: 79.6
Acetyl-CoA: 67.8
3-Methylbutyryl-CoA: 45.5
fatty_acid: 44.9
inactive: 28.4

PKS_AT (2166..2464): emal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 95.8%; Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 95.8%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 222.7
Methylmalonyl-CoA: 202.7
Methoxymalonyl-CoA: 183.9
trans-1,2-CPDA: 144.9
2-Methylbutyryl-CoA: 137.2
Propionyl-CoA: 104.7
Malonyl-CoA: 97.4
CHC-CoA: 89.5
Benzoyl-CoA: 56.8
Isobutyryl-CoA: 55.8
Acetyl-CoA: 38.9
3-Methylbutyryl-CoA: 30.3
fatty_acid: 29.1
inactive: 27.0

PKS_AT (3812..4103): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; 2-Methylbutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.0
inactive: 106.4
Methoxymalonyl-CoA: 62.2
Methylmalonyl-CoA: 55.8
Propionyl-CoA: 35.8
Ethylmalonyl-CoA: 31.9
Isobutyryl-CoA: 24.1
Acetyl-CoA: 23.0
CHC-CoA: 19.1
2-Methylbutyryl-CoA: 18.5
Benzoyl-CoA: 10.8
fatty_acid: 6.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

ADC79618.1: pk - mmal

PKS_AT (605..903): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 232.8
Methylmalonyl-CoA: 228.1
Methoxymalonyl-CoA: 198.0
2-Methylbutyryl-CoA: 179.8
trans-1,2-CPDA: 149.0
Propionyl-CoA: 128.2
Malonyl-CoA: 121.3
CHC-CoA: 117.3
Benzoyl-CoA: 83.6
Isobutyryl-CoA: 79.8
Acetyl-CoA: 46.2
3-Methylbutyryl-CoA: 44.6
fatty_acid: 41.1
inactive: 33.1

PKS_AT (2376..2670): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.2
Ethylmalonyl-CoA: 208.9
Methoxymalonyl-CoA: 176.9
2-Methylbutyryl-CoA: 164.3
trans-1,2-CPDA: 128.4
Propionyl-CoA: 125.1
CHC-CoA: 101.3
Malonyl-CoA: 96.8
Isobutyryl-CoA: 94.9
Benzoyl-CoA: 78.5
Acetyl-CoA: 63.5
fatty_acid: 46.9
3-Methylbutyryl-CoA: 37.7
inactive: 28.1

ADC79619.1: mmal - mmal

PKS_AT (622..921): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 213.4
Ethylmalonyl-CoA: 213.0
Methoxymalonyl-CoA: 180.8
2-Methylbutyryl-CoA: 145.5
trans-1,2-CPDA: 116.9
Propionyl-CoA: 107.5
Malonyl-CoA: 105.6
CHC-CoA: 104.0
Isobutyryl-CoA: 81.4
Benzoyl-CoA: 66.0
3-Methylbutyryl-CoA: 57.9
Acetyl-CoA: 52.1
inactive: 39.0
fatty_acid: 32.4

PKS_AT (2210..2502): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.8
Ethylmalonyl-CoA: 201.7
Methoxymalonyl-CoA: 171.2
2-Methylbutyryl-CoA: 141.8
trans-1,2-CPDA: 135.0
Propionyl-CoA: 109.4
CHC-CoA: 97.1
Malonyl-CoA: 92.8
Isobutyryl-CoA: 83.3
Benzoyl-CoA: 77.6
Acetyl-CoA: 48.1
fatty_acid: 39.4
3-Methylbutyryl-CoA: 39.4
inactive: 31.0

ADC79620.1: mmal

PKS_AT (567..862): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 201.3
Ethylmalonyl-CoA: 183.7
Methoxymalonyl-CoA: 169.7
2-Methylbutyryl-CoA: 157.8
trans-1,2-CPDA: 129.1
Propionyl-CoA: 114.2
Malonyl-CoA: 102.8
CHC-CoA: 97.5
Isobutyryl-CoA: 71.2
Benzoyl-CoA: 64.5
fatty_acid: 60.3
Acetyl-CoA: 35.7
3-Methylbutyryl-CoA: 33.6
inactive: 27.3

ADC79629.1: pk

PKS_AT (16..277): pk

ATSignature: (unknown)

No matches above 50%

minowa: Isobutyryl-CoA

Prediction, score:

Isobutyryl-CoA: 14.9
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ADC79630.1: X

AMP-binding (70..282): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ELGNRS-FGK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor