BGC0000028 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000028 (Streptomyces lohii)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	74,009	bafilomycin B1	PKS:Type I	100%

BGC0000028 - Region 1 - T1PKS

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Location: 1 - 74,009 nt. (total: 74,009 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 74,010
Label		BGC0000028

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000028	1.00	Polyketide	bafilomycin B1	Streptomyces lohii
BGC0000016	0.40	Other	amphotericin B	Streptomyces nodosus
BGC0001348	0.37	Polyketide	JBIR-100	Streptomyces varsoviensis
BGC0000041	0.36	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000122	0.34	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0001137	0.32	Alkaloid	marinacarboline A, marinacarboline B, marinacarboline C, marinacarboline D	Marinactinospora thermotolerans
BGC0000113	0.31	Polyketide	niddamycin	Streptomyces caelestis
BGC0001163	0.31	Polyketide	1-heptadecene	Moorea producens 3L
BGC0000706	0.31	Saccharide	kanamycin	Streptomyces kanamyceticus
BGC0002597	0.30	NRP, Polyketide	nocuolactylates	Nodularia sp. LEGE 06071

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000028	1.00	Polyketide	bafilomycin B1	Streptomyces lohii
BGC0000016	0.72	Other	amphotericin B	Streptomyces nodosus
BGC0001348	0.70	Polyketide	JBIR-100	Streptomyces varsoviensis
BGC0000041	0.68	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000122	0.67	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0001137	0.65	Alkaloid	marinacarboline A, marinacarboline B, marinacarboline C, marinacarboline D	Marinactinospora thermotolerans
BGC0000113	0.65	Polyketide	niddamycin	Streptomyces caelestis
BGC0001163	0.64	Polyketide	1-heptadecene	Moorea producens 3L
BGC0000706	0.64	Saccharide	kanamycin	Streptomyces kanamyceticus
BGC0002597	0.64	NRP, Polyketide	nocuolactylates	Nodularia sp. LEGE 06071

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 74009:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ohmal - ohmal - Me-mal) + (Me-ohmal - ohemal - ccmal) + (pk - Me-redmal) + (Me-ohmal - Me-ccmal) + (Me-ccmal)
Non-colinear ordering:: ADC79616.1 + ADC79617.1 + ADC79618.1 + ADC79619.1 + ADC79620.1

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ADC79613.1

AMP-binding (10..414): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TFCMFGGPVK
Nearest Stachelhaus code(s):: nLev GFCMYGGCSK (74% 8Å match)
Stachelhaus code match:: 60% (weak)

ADC79614.1

AMP-binding (15..418): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SSTLTVVFVK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

ADC79616.1

PKS_AT (106..405): mmal

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 79.2%; Methoxymalonyl-CoA: 70.8%; Methylmalonyl-CoA: 70.8%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 161.1
Ethylmalonyl-CoA: 157.2
2-Methylbutyryl-CoA: 153.9
Methoxymalonyl-CoA: 134.5
Propionyl-CoA: 134.0
CHC-CoA: 94.5
trans-1,2-CPDA: 87.3
Malonyl-CoA: 84.7
Isobutyryl-CoA: 80.4
Benzoyl-CoA: 46.1
Acetyl-CoA: 34.9
3-Methylbutyryl-CoA: 30.8
inactive: 23.0
fatty_acid: 22.1

PKS_AT (1120..1414): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 211.9
Ethylmalonyl-CoA: 206.7
Methoxymalonyl-CoA: 168.8
2-Methylbutyryl-CoA: 162.1
trans-1,2-CPDA: 135.7
Propionyl-CoA: 119.4
CHC-CoA: 99.3
Malonyl-CoA: 87.5
Benzoyl-CoA: 81.7
Isobutyryl-CoA: 73.9
Acetyl-CoA: 55.4
fatty_acid: 41.1
3-Methylbutyryl-CoA: 32.0
inactive: 20.8

PKS_AT (2714..3015): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 235.9
inactive: 116.4
Methoxymalonyl-CoA: 72.9
Methylmalonyl-CoA: 60.1
Propionyl-CoA: 45.7
Isobutyryl-CoA: 36.7
Benzoyl-CoA: 21.5
Ethylmalonyl-CoA: 21.3
2-Methylbutyryl-CoA: 17.5
Acetyl-CoA: 17.1
CHC-CoA: 12.0
fatty_acid: 9.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (4311..4607): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 201.0
Ethylmalonyl-CoA: 198.4
Methoxymalonyl-CoA: 165.9
2-Methylbutyryl-CoA: 142.8
trans-1,2-CPDA: 122.0
Propionyl-CoA: 113.3
CHC-CoA: 91.3
Malonyl-CoA: 81.6
Isobutyryl-CoA: 75.6
Benzoyl-CoA: 69.2
Acetyl-CoA: 47.8
3-Methylbutyryl-CoA: 42.0
fatty_acid: 37.7
inactive: 29.7

ADC79617.1

PKS_AT (578..878): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.6
Ethylmalonyl-CoA: 205.1
Methoxymalonyl-CoA: 174.1
2-Methylbutyryl-CoA: 165.4
trans-1,2-CPDA: 119.5
Propionyl-CoA: 115.7
CHC-CoA: 106.6
Malonyl-CoA: 93.4
Isobutyryl-CoA: 80.9
Benzoyl-CoA: 79.6
Acetyl-CoA: 67.8
3-Methylbutyryl-CoA: 45.5
fatty_acid: 44.9
inactive: 28.4

PKS_AT (2166..2464): emal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 95.8%; Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 95.8%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 222.7
Methylmalonyl-CoA: 202.7
Methoxymalonyl-CoA: 183.9
trans-1,2-CPDA: 144.9
2-Methylbutyryl-CoA: 137.2
Propionyl-CoA: 104.7
Malonyl-CoA: 97.4
CHC-CoA: 89.5
Benzoyl-CoA: 56.8
Isobutyryl-CoA: 55.8
Acetyl-CoA: 38.9
3-Methylbutyryl-CoA: 30.3
fatty_acid: 29.1
inactive: 27.0

PKS_AT (3812..4103): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; 2-Methylbutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.0
inactive: 106.4
Methoxymalonyl-CoA: 62.2
Methylmalonyl-CoA: 55.8
Propionyl-CoA: 35.8
Ethylmalonyl-CoA: 31.9
Isobutyryl-CoA: 24.1
Acetyl-CoA: 23.0
CHC-CoA: 19.1
2-Methylbutyryl-CoA: 18.5
Benzoyl-CoA: 10.8
fatty_acid: 6.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

ADC79618.1

PKS_AT (605..903): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 232.8
Methylmalonyl-CoA: 228.1
Methoxymalonyl-CoA: 198.0
2-Methylbutyryl-CoA: 179.8
trans-1,2-CPDA: 149.0
Propionyl-CoA: 128.2
Malonyl-CoA: 121.3
CHC-CoA: 117.3
Benzoyl-CoA: 83.6
Isobutyryl-CoA: 79.8
Acetyl-CoA: 46.2
3-Methylbutyryl-CoA: 44.6
fatty_acid: 41.1
inactive: 33.1

PKS_AT (2376..2670): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.2
Ethylmalonyl-CoA: 208.9
Methoxymalonyl-CoA: 176.9
2-Methylbutyryl-CoA: 164.3
trans-1,2-CPDA: 128.4
Propionyl-CoA: 125.1
CHC-CoA: 101.3
Malonyl-CoA: 96.8
Isobutyryl-CoA: 94.9
Benzoyl-CoA: 78.5
Acetyl-CoA: 63.5
fatty_acid: 46.9
3-Methylbutyryl-CoA: 37.7
inactive: 28.1

ADC79619.1

PKS_AT (622..921): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 213.4
Ethylmalonyl-CoA: 213.0
Methoxymalonyl-CoA: 180.8
2-Methylbutyryl-CoA: 145.5
trans-1,2-CPDA: 116.9
Propionyl-CoA: 107.5
Malonyl-CoA: 105.6
CHC-CoA: 104.0
Isobutyryl-CoA: 81.4
Benzoyl-CoA: 66.0
3-Methylbutyryl-CoA: 57.9
Acetyl-CoA: 52.1
inactive: 39.0
fatty_acid: 32.4

PKS_AT (2210..2502): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.8
Ethylmalonyl-CoA: 201.7
Methoxymalonyl-CoA: 171.2
2-Methylbutyryl-CoA: 141.8
trans-1,2-CPDA: 135.0
Propionyl-CoA: 109.4
CHC-CoA: 97.1
Malonyl-CoA: 92.8
Isobutyryl-CoA: 83.3
Benzoyl-CoA: 77.6
Acetyl-CoA: 48.1
fatty_acid: 39.4
3-Methylbutyryl-CoA: 39.4
inactive: 31.0

ADC79620.1

PKS_AT (567..862): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 201.3
Ethylmalonyl-CoA: 183.7
Methoxymalonyl-CoA: 169.7
2-Methylbutyryl-CoA: 157.8
trans-1,2-CPDA: 129.1
Propionyl-CoA: 114.2
Malonyl-CoA: 102.8
CHC-CoA: 97.5
Isobutyryl-CoA: 71.2
Benzoyl-CoA: 64.5
fatty_acid: 60.3
Acetyl-CoA: 35.7
3-Methylbutyryl-CoA: 33.6
inactive: 27.3

ADC79629.1

PKS_AT (16..277): pk

ATSignature: (unknown)

No matches above 50%

minowa: Isobutyryl-CoA

Prediction, score:

Isobutyryl-CoA: 14.9
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ADC79630.1

AMP-binding (70..282): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ELGNRS-FGK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)