BGC0000032 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000032 (Streptomyces chartreusis NRRL 3882)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	68,313	A23187	PKS	100%

BGC0000032 - Region 1 - T1PKS

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Location: 1 - 68,313 nt. (total: 68,313 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 68,314
Label		BGC0000032

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000032	1.00	Polyketide	A23187	Streptomyces chartreusis NRRL 3882
BGC0000016	0.40	Other	amphotericin B	Streptomyces nodosus
BGC0000041	0.40	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001164	0.35	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000867	0.33	Other	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0000343	0.33	NRP	enterobactin	Pseudomonas sp. J465
BGC0001925	0.32	Alkaloid	altemicidin, SB-203207, SB-203208	Streptomyces sp.
BGC0001163	0.32	Polyketide	1-heptadecene	Moorea producens 3L
BGC0002729	0.31	Polyketide	sordarial	Neurospora crassa OR74A
BGC0001749	0.31	Polyketide	epipyriculol	Pyricularia oryzae 70-15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000032	1.00	Polyketide	A23187	Streptomyces chartreusis NRRL 3882
BGC0000016	0.72	Other	amphotericin B	Streptomyces nodosus
BGC0000041	0.72	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001164	0.68	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000867	0.67	Other	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0000343	0.66	NRP	enterobactin	Pseudomonas sp. J465
BGC0001925	0.66	Alkaloid	altemicidin, SB-203207, SB-203208	Streptomyces sp.
BGC0001163	0.65	Polyketide	1-heptadecene	Moorea producens 3L
BGC0002729	0.65	Polyketide	sordarial	Neurospora crassa OR74A
BGC0001749	0.64	Polyketide	epipyriculol	Pyricularia oryzae 70-15

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 68313:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ohmal) + (Me-redmal) + (ccmal - Me-redmal) + (ohmal)
Non-colinear ordering:: SchA1 + SchA2 + SchA5 + SchA4

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AEH42471.1

AMP-binding (16..428): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SRTESTLPVK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

SchA1

PKS_AT (543..839): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 75.0%; Malonyl-CoA: 70.8%; 2-Methylbutyryl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 133.4
Malonyl-CoA: 127.4
Methoxymalonyl-CoA: 113.1
Ethylmalonyl-CoA: 92.4
Isobutyryl-CoA: 85.5
Propionyl-CoA: 78.7
2-Methylbutyryl-CoA: 70.0
Acetyl-CoA: 60.4
Benzoyl-CoA: 51.1
trans-1,2-CPDA: 47.7
CHC-CoA: 47.6
fatty_acid: 46.7
inactive: 42.9
3-Methylbutyryl-CoA: 32.4

PKS_AT (1898..2194): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 181.7
Ethylmalonyl-CoA: 156.4
Methoxymalonyl-CoA: 134.7
2-Methylbutyryl-CoA: 109.0
trans-1,2-CPDA: 106.6
Propionyl-CoA: 84.7
Malonyl-CoA: 82.6
CHC-CoA: 67.9
Isobutyryl-CoA: 44.7
Benzoyl-CoA: 41.5
inactive: 26.1
fatty_acid: 19.8
3-Methylbutyryl-CoA: 17.7
Acetyl-CoA: 16.9

SchA2

PKS_AT (447..743): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 184.7
Ethylmalonyl-CoA: 159.0
Methoxymalonyl-CoA: 135.8
2-Methylbutyryl-CoA: 115.2
trans-1,2-CPDA: 108.0
Propionyl-CoA: 87.8
Malonyl-CoA: 84.6
CHC-CoA: 68.7
Isobutyryl-CoA: 45.8
Benzoyl-CoA: 42.3
inactive: 26.9
3-Methylbutyryl-CoA: 23.8
Acetyl-CoA: 21.2
fatty_acid: 19.7

SchN2

Search NORINE for peptide: strict or relaxed

AMP-binding (8..400): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLLYLALVCK
Nearest Stachelhaus code(s):: Pro DLLYLALVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

SchA3

PKS_AT (462..723): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 34.0
Methoxymalonyl-CoA: 20.4
Methylmalonyl-CoA: 14.9
Acetyl-CoA: 8.6
Propionyl-CoA: 2.1
trans-1,2-CPDA: 0.8
CHC-CoA: 0.4
inactive: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -3.5
Ethylmalonyl-CoA: -3.6

SchA4

PKS_AT (562..858): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 187.9
inactive: 88.3
Methoxymalonyl-CoA: 64.4
Methylmalonyl-CoA: 61.4
Isobutyryl-CoA: 35.6
Ethylmalonyl-CoA: 33.2
Propionyl-CoA: 25.9
Benzoyl-CoA: 22.4
Acetyl-CoA: 19.1
fatty_acid: 17.3
2-Methylbutyryl-CoA: 14.9
CHC-CoA: 13.5
trans-1,2-CPDA: 11.3
3-Methylbutyryl-CoA: 0.0

SchA5

PKS_AT (588..884): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 187.9
inactive: 88.3
Methoxymalonyl-CoA: 64.4
Methylmalonyl-CoA: 61.4
Isobutyryl-CoA: 35.6
Ethylmalonyl-CoA: 33.2
Propionyl-CoA: 25.9
Benzoyl-CoA: 22.4
Acetyl-CoA: 19.1
fatty_acid: 17.3
2-Methylbutyryl-CoA: 14.9
CHC-CoA: 13.5
trans-1,2-CPDA: 11.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (2365..2661): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 184.7
Ethylmalonyl-CoA: 159.0
Methoxymalonyl-CoA: 135.8
2-Methylbutyryl-CoA: 115.2
trans-1,2-CPDA: 108.0
Propionyl-CoA: 87.8
Malonyl-CoA: 84.6
CHC-CoA: 68.7
Isobutyryl-CoA: 45.8
Benzoyl-CoA: 42.3
inactive: 26.9
3-Methylbutyryl-CoA: 23.8
Acetyl-CoA: 21.2
fatty_acid: 19.7