BGC0000034 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000034 (Streptomyces sp. FR-008)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS-like	1	138,203	candicidin	NRPS:Type I+PKS	100%

BGC0000034 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 138,203 nt. (total: 138,203 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 138,204
Label		BGC0000034

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000034	1.35	NRP, Polyketide	candicidin	Streptomyces sp. FR-008
BGC0000016	0.81	Other	amphotericin B	Streptomyces nodosus
BGC0002031	0.68	Alkaloid	kitasetaline	Kitasatospora setae KM-6054
BGC0002631	0.63	RiPP	myxarylin	Pyxidicoccus fallax
BGC0000138	0.59	Polyketide	rimocidin	Streptomyces diastaticus
BGC0000122	0.58	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0001075	0.55	Terpene, Polyketide	aurachin RE	Rhodococcus erythropolis
BGC0000892	0.54	Other	caryoynencin	Burkholderia gladioli BSR3
BGC0002597	0.50	NRP, Polyketide	nocuolactylates	Nodularia sp. LEGE 06071
BGC0001817	0.50	Other	vancosamine	Streptomyces sp. WAC2288

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000034	1.00	NRP, Polyketide	candicidin	Streptomyces sp. FR-008
BGC0000016	0.72	Other	amphotericin B	Streptomyces nodosus
BGC0000122	0.68	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0002031	0.67	Alkaloid	kitasetaline	Kitasatospora setae KM-6054
BGC0001164	0.65	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000996	0.65	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0000138	0.65	Polyketide	rimocidin	Streptomyces diastaticus
BGC0002104	0.65	NRP, Polyketide	eurocidin D, eurocidin E	Streptomyces eurocidicus ATCC 27428
BGC0001163	0.65	Polyketide	1-heptadecene	Moorea producens 3L
BGC0002631	0.65	RiPP	myxarylin	Pyxidicoccus fallax

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 926 - 138203:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal - ohmal - pk - ohmal - ohmal - ohmal) + (redmal - redmal - ohmal - redmal) + (ccmal) + (Me-ohmal - Me-redmal - Me-ccmal) + (ccmal - ccmal - ccmal - ccmal - ccmal - ccmal) + (X - mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

fscA

AMP-binding (30..440): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: --QVLAVNGK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

PKS_AT (1204..1498): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.7
inactive: 87.0
Methoxymalonyl-CoA: 62.5
Methylmalonyl-CoA: 54.0
Isobutyryl-CoA: 46.8
Acetyl-CoA: 28.7
Ethylmalonyl-CoA: 27.8
Propionyl-CoA: 26.4
2-Methylbutyryl-CoA: 21.2
Benzoyl-CoA: 18.8
fatty_acid: 18.4
CHC-CoA: 9.7
trans-1,2-CPDA: 8.7
3-Methylbutyryl-CoA: 6.6

fscC

PKS_AT (578..870): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 231.6
inactive: 106.2
Methoxymalonyl-CoA: 54.7
Methylmalonyl-CoA: 50.1
Propionyl-CoA: 29.8
Ethylmalonyl-CoA: 20.9
Benzoyl-CoA: 20.3
Isobutyryl-CoA: 20.0
Acetyl-CoA: 17.7
fatty_acid: 14.3
trans-1,2-CPDA: 12.3
2-Methylbutyryl-CoA: 11.4
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2320..2612): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 225.6
inactive: 112.3
Methoxymalonyl-CoA: 57.8
Methylmalonyl-CoA: 48.6
Propionyl-CoA: 28.5
Isobutyryl-CoA: 24.0
Benzoyl-CoA: 22.3
Acetyl-CoA: 21.5
Ethylmalonyl-CoA: 14.4
2-Methylbutyryl-CoA: 13.5
fatty_acid: 10.2
trans-1,2-CPDA: 6.0
CHC-CoA: 2.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (4061..4351): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.3
inactive: 99.0
Methoxymalonyl-CoA: 54.0
Methylmalonyl-CoA: 51.1
Acetyl-CoA: 26.2
Propionyl-CoA: 25.5
Benzoyl-CoA: 13.9
Isobutyryl-CoA: 12.4
Ethylmalonyl-CoA: 11.4
CHC-CoA: 11.3
2-Methylbutyryl-CoA: 10.4
fatty_acid: 8.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (5777..6069): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 228.9
inactive: 112.2
Methoxymalonyl-CoA: 56.4
Methylmalonyl-CoA: 53.7
Propionyl-CoA: 31.4
Acetyl-CoA: 24.0
Benzoyl-CoA: 19.8
trans-1,2-CPDA: 18.6
Isobutyryl-CoA: 18.2
Ethylmalonyl-CoA: 17.1
2-Methylbutyryl-CoA: 13.3
fatty_acid: 11.6
CHC-CoA: 3.8
3-Methylbutyryl-CoA: 0.0

PKS_AT (7492..7783): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 214.4
inactive: 98.5
Methoxymalonyl-CoA: 52.6
Methylmalonyl-CoA: 44.6
Propionyl-CoA: 26.3
Acetyl-CoA: 20.9
Isobutyryl-CoA: 16.2
Benzoyl-CoA: 11.5
Ethylmalonyl-CoA: 9.6
trans-1,2-CPDA: 8.8
fatty_acid: 8.8
2-Methylbutyryl-CoA: 8.6
CHC-CoA: 2.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (9332..9621): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.5
inactive: 89.0
Methoxymalonyl-CoA: 40.7
Methylmalonyl-CoA: 40.0
Propionyl-CoA: 29.0
Acetyl-CoA: 25.1
Ethylmalonyl-CoA: 16.8
Isobutyryl-CoA: 9.8
Benzoyl-CoA: 9.8
2-Methylbutyryl-CoA: 6.6
CHC-CoA: 2.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

fscB

PKS_AT (573..870): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 216.7
Ethylmalonyl-CoA: 212.0
Methoxymalonyl-CoA: 168.8
2-Methylbutyryl-CoA: 155.9
trans-1,2-CPDA: 126.5
Propionyl-CoA: 120.3
Malonyl-CoA: 98.3
CHC-CoA: 95.6
Isobutyryl-CoA: 68.3
Benzoyl-CoA: 65.0
Acetyl-CoA: 51.2
fatty_acid: 44.1
inactive: 33.8
3-Methylbutyryl-CoA: 33.6

PKS_AT (2141..2438): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 216.7
Ethylmalonyl-CoA: 212.0
Methoxymalonyl-CoA: 168.8
2-Methylbutyryl-CoA: 155.9
trans-1,2-CPDA: 126.5
Propionyl-CoA: 120.3
Malonyl-CoA: 98.3
CHC-CoA: 95.6
Isobutyryl-CoA: 68.3
Benzoyl-CoA: 65.0
Acetyl-CoA: 51.2
fatty_acid: 44.1
inactive: 33.8
3-Methylbutyryl-CoA: 33.6

PKS_AT (4249..4546): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 206.1
Ethylmalonyl-CoA: 202.9
Methoxymalonyl-CoA: 169.3
2-Methylbutyryl-CoA: 131.7
trans-1,2-CPDA: 129.8
Propionyl-CoA: 108.2
Malonyl-CoA: 103.0
CHC-CoA: 98.2
Isobutyryl-CoA: 71.5
Benzoyl-CoA: 57.5
Acetyl-CoA: 41.0
inactive: 38.2
fatty_acid: 30.1
3-Methylbutyryl-CoA: 28.7

fscF

PKS_AT (547..843): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 226.9
inactive: 118.3
Methoxymalonyl-CoA: 63.2
Methylmalonyl-CoA: 60.7
Isobutyryl-CoA: 26.9
Propionyl-CoA: 26.3
Benzoyl-CoA: 25.2
fatty_acid: 23.1
Ethylmalonyl-CoA: 20.6
CHC-CoA: 20.5
Acetyl-CoA: 20.2
trans-1,2-CPDA: 13.1
2-Methylbutyryl-CoA: 10.9
3-Methylbutyryl-CoA: 0.0

fscE

PKS_AT (550..845): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 233.9
inactive: 116.1
Methoxymalonyl-CoA: 63.1
Methylmalonyl-CoA: 56.5
Isobutyryl-CoA: 27.3
Propionyl-CoA: 24.2
Benzoyl-CoA: 22.9
Acetyl-CoA: 21.2
Ethylmalonyl-CoA: 14.7
fatty_acid: 11.4
2-Methylbutyryl-CoA: 8.7
CHC-CoA: 8.4
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2592..2891): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.5
inactive: 93.3
Methoxymalonyl-CoA: 58.2
Methylmalonyl-CoA: 46.0
Benzoyl-CoA: 20.3
Isobutyryl-CoA: 19.8
Ethylmalonyl-CoA: 17.0
Acetyl-CoA: 16.3
fatty_acid: 13.3
Propionyl-CoA: 11.2
CHC-CoA: 8.8
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (4635..4931): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 231.4
inactive: 113.9
Methoxymalonyl-CoA: 69.2
Methylmalonyl-CoA: 61.2
Propionyl-CoA: 37.6
Isobutyryl-CoA: 28.3
fatty_acid: 22.5
Ethylmalonyl-CoA: 22.5
Benzoyl-CoA: 22.4
Acetyl-CoA: 21.2
2-Methylbutyryl-CoA: 16.1
CHC-CoA: 15.9
trans-1,2-CPDA: 12.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (6160..6458): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 221.5
inactive: 109.3
Methoxymalonyl-CoA: 63.0
Methylmalonyl-CoA: 55.4
Propionyl-CoA: 25.5
CHC-CoA: 22.1
Acetyl-CoA: 21.0
2-Methylbutyryl-CoA: 20.3
Benzoyl-CoA: 20.2
Ethylmalonyl-CoA: 19.7
Isobutyryl-CoA: 16.8
fatty_acid: 16.0
trans-1,2-CPDA: 9.6
3-Methylbutyryl-CoA: 0.0

fscD

PKS_AT (562..863): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.6
inactive: 91.1
Methoxymalonyl-CoA: 52.8
Methylmalonyl-CoA: 42.8
Isobutyryl-CoA: 27.8
Propionyl-CoA: 26.7
Ethylmalonyl-CoA: 20.5
Benzoyl-CoA: 20.3
Acetyl-CoA: 17.4
2-Methylbutyryl-CoA: 16.2
CHC-CoA: 13.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2315..2622): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 215.9
inactive: 103.3
Methoxymalonyl-CoA: 57.9
Methylmalonyl-CoA: 45.5
Propionyl-CoA: 34.7
Acetyl-CoA: 21.2
Isobutyryl-CoA: 20.3
Benzoyl-CoA: 16.9
2-Methylbutyryl-CoA: 16.4
fatty_acid: 7.4
CHC-CoA: 7.3
Ethylmalonyl-CoA: 6.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (3874..4171): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 224.9
Methylmalonyl-CoA: 223.8
Methoxymalonyl-CoA: 181.4
2-Methylbutyryl-CoA: 163.1
trans-1,2-CPDA: 134.0
Propionyl-CoA: 118.2
CHC-CoA: 117.5
Malonyl-CoA: 109.9
Isobutyryl-CoA: 84.8
Benzoyl-CoA: 67.1
Acetyl-CoA: 52.7
3-Methylbutyryl-CoA: 37.3
inactive: 32.4
fatty_acid: 31.4

PKS_AT (5410..5717): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.1
inactive: 112.2
Methoxymalonyl-CoA: 61.9
Methylmalonyl-CoA: 54.9
Propionyl-CoA: 21.6
Isobutyryl-CoA: 16.8
Ethylmalonyl-CoA: 13.8
Acetyl-CoA: 13.6
Benzoyl-CoA: 10.6
fatty_acid: 9.6
CHC-CoA: 9.0
3-Methylbutyryl-CoA: 6.6
trans-1,2-CPDA: 3.5
2-Methylbutyryl-CoA: -0.2

PKS_AT (6937..7236): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 238.0
inactive: 117.7
Methoxymalonyl-CoA: 64.5
Methylmalonyl-CoA: 58.6
Propionyl-CoA: 27.4
Benzoyl-CoA: 22.0
Isobutyryl-CoA: 20.3
Acetyl-CoA: 19.7
Ethylmalonyl-CoA: 15.5
fatty_acid: 15.2
CHC-CoA: 12.8
2-Methylbutyryl-CoA: 12.6
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (8466..8767): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 224.8
inactive: 103.6
Methoxymalonyl-CoA: 65.5
Methylmalonyl-CoA: 59.3
Isobutyryl-CoA: 29.0
Ethylmalonyl-CoA: 26.8
Propionyl-CoA: 26.2
Benzoyl-CoA: 19.7
Acetyl-CoA: 18.3
fatty_acid: 17.7
CHC-CoA: 16.8
2-Methylbutyryl-CoA: 11.6
trans-1,2-CPDA: 11.4
3-Methylbutyryl-CoA: 0.0