BGC0000055 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000055 (Saccharopolyspora erythraea NRRL 2338)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	72,299	High	erythromycin A/erythromycin B/erythromycin C/erythromycin D	PKS:Type I+saccharide:hybrid/tailoring

BGC0000055 - Region 1 - T1PKS

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Location: 1 - 72,299 nt. (total: 72,299 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 72,300
Label		BGC0000055

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

SACE_0713: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

SACE_0723: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

SACE_0724: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

SACE_0725: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

SACE_0727: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

SACE_0730: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: aminotransferase glycosyltransferase methyltransferase

SACE_0716: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

SACE_0718: methyltransferase

smcogs: SMCOG1248: methyltransferase

SACE_0719: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

SACE_0726: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

SACE_0728: methyltransferase

smcogs: SMCOG1089: methyltransferase

SACE_0734: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

Hydrolases: esterase

SACE_0729: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

SACE_0720: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

SACE_0721: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000055.5	1.00	PKS, saccharide	erythromycin A, erythromycin B, erythromycin C, erythromycin D	Saccharopolyspora erythraea NRRL 2338
BGC0000041.3	0.65	PKS	oronofacic acid	Pseudomonas syringae
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000067.5	0.60	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002084.4	0.58	ribosomal	biarylitide YYH	Planomonospora sp. ID82291
BGC0001817.5	0.56	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0000054.5	0.55	PKS	erythromycin	Aeromicrobium erythreum
BGC0001051.5	0.55	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0000016.4	0.52	other (other)	amphotericin B	Streptomyces nodosus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000055.5	1.00	PKS, saccharide	erythromycin A, erythromycin B, erythromycin C, erythromycin D	Saccharopolyspora erythraea NRRL 2338
BGC0000041.3	0.65	PKS	oronofacic acid	Pseudomonas syringae
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000067.5	0.60	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002084.4	0.58	ribosomal	biarylitide YYH	Planomonospora sp. ID82291
BGC0001817.5	0.56	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0000054.5	0.55	PKS	erythromycin	Aeromicrobium erythreum
BGC0001051.5	0.55	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0000016.4	0.52	other (other)	amphotericin B	Streptomyces nodosus

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 72299:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - Me-ohmal - Me-ohmal) + (Me-ohmal - Me-redmal) + (Me-ohmal - Me-ohmal)
Non-colinear ordering:: SACE_0721 + SACE_0723 + SACE_0724

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

SACE_0721: pk - mmal - mmal

PKS_AT (110..404): pk

ATSignature: Propionyl-CoA

Top 3 matches:: Propionyl-CoA: 100.0%; 2-Methylbutyryl-CoA: 79.2%; Methylmalonyl-CoA: 70.8%

minowa: Propionyl-CoA

Prediction, score:

Propionyl-CoA: 229.4
2-Methylbutyryl-CoA: 182.9
Methylmalonyl-CoA: 140.2
Ethylmalonyl-CoA: 126.8
Methoxymalonyl-CoA: 117.9
CHC-CoA: 92.1
trans-1,2-CPDA: 86.4
Malonyl-CoA: 73.7
Acetyl-CoA: 51.1
fatty_acid: 46.2
Isobutyryl-CoA: 41.8
Benzoyl-CoA: 31.9
3-Methylbutyryl-CoA: 26.4
inactive: 22.0

PKS_AT (1085..1386): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 218.9
Ethylmalonyl-CoA: 207.0
Methoxymalonyl-CoA: 181.8
2-Methylbutyryl-CoA: 138.0
trans-1,2-CPDA: 125.3
Propionyl-CoA: 118.8
Malonyl-CoA: 102.2
CHC-CoA: 82.9
Isobutyryl-CoA: 77.6
Benzoyl-CoA: 59.9
Acetyl-CoA: 51.0
fatty_acid: 44.9
3-Methylbutyryl-CoA: 35.0
inactive: 31.7

PKS_AT (2563..2861): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.3
Ethylmalonyl-CoA: 207.3
Methoxymalonyl-CoA: 168.2
2-Methylbutyryl-CoA: 151.4
trans-1,2-CPDA: 116.0
Propionyl-CoA: 115.1
Malonyl-CoA: 100.9
CHC-CoA: 95.8
Isobutyryl-CoA: 69.9
Benzoyl-CoA: 63.1
Acetyl-CoA: 40.3
3-Methylbutyryl-CoA: 35.9
fatty_acid: 23.1
inactive: 21.3

SACE_0723: mmal - mmal

PKS_AT (561..860): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 208.8
Ethylmalonyl-CoA: 200.7
Methoxymalonyl-CoA: 170.0
2-Methylbutyryl-CoA: 135.5
trans-1,2-CPDA: 112.4
Malonyl-CoA: 100.4
Propionyl-CoA: 98.1
CHC-CoA: 89.1
Isobutyryl-CoA: 81.7
Benzoyl-CoA: 66.6
inactive: 45.7
Acetyl-CoA: 41.4
3-Methylbutyryl-CoA: 39.3
fatty_acid: 25.9

PKS_AT (2015..2311): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 211.9
Ethylmalonyl-CoA: 190.4
Methoxymalonyl-CoA: 170.2
2-Methylbutyryl-CoA: 140.0
trans-1,2-CPDA: 121.2
Propionyl-CoA: 100.9
Malonyl-CoA: 92.3
CHC-CoA: 81.0
Isobutyryl-CoA: 78.4
Benzoyl-CoA: 61.9
Acetyl-CoA: 58.7
inactive: 35.5
fatty_acid: 35.2
3-Methylbutyryl-CoA: 26.7

SACE_0724: mmal - mmal

PKS_AT (555..852): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 216.1
Ethylmalonyl-CoA: 206.8
Methoxymalonyl-CoA: 173.9
2-Methylbutyryl-CoA: 144.5
trans-1,2-CPDA: 129.9
Propionyl-CoA: 111.3
Malonyl-CoA: 105.3
CHC-CoA: 99.0
Isobutyryl-CoA: 98.0
Benzoyl-CoA: 84.1
Acetyl-CoA: 62.6
3-Methylbutyryl-CoA: 47.4
fatty_acid: 42.6
inactive: 31.6

PKS_AT (2018..2309): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 211.2
Ethylmalonyl-CoA: 202.8
Methoxymalonyl-CoA: 174.6
2-Methylbutyryl-CoA: 140.8
Malonyl-CoA: 120.1
trans-1,2-CPDA: 120.0
Propionyl-CoA: 106.2
CHC-CoA: 90.8
Isobutyryl-CoA: 67.3
Benzoyl-CoA: 53.1
Acetyl-CoA: 37.5
fatty_acid: 33.5
inactive: 28.5
3-Methylbutyryl-CoA: 21.1

Link to PARAS substrate predictor