LOCUS       BGC0000056              6689 bp    DNA     linear   BCT 25-JUL-2016
DEFINITION  Actinomadura verrucosospora strain ATCC 39334 esperamicin enediyne
            iterative type I polyketide synthase gene locus, partial sequence.
ACCESSION   BGC0000056
VERSION     BGC0000056
KEYWORDS    .
SOURCE      Actinomadura verrucosospora
  ORGANISM  Actinomadura verrucosospora
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 12:41:11
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            <1..890
                     /gene="espT3"
     source          1..6689
                     /db_xref="ATCC:39334"
                     /db_xref="taxon:46165"
                     /mol_type="genomic DNA"
                     /organism="Actinomadura verrucosospora"
                     /strain="ATCC 39334"
     protocluster    1..6689
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[886:6481]"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      887..6481
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     cand_cluster    1..6689
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..6689
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000056"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6689
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             <1..890
                     /codon_start=3
                     /gene="espT3"
                     /note="putative; similar to UNBU"
                     /product="EspT3"
                     /protein_id="AAP92147.1"
                     /transl_table=11
                     /translation="MHRCVGFEQFWATPLAAMAVAYVMELGLETLDAWATRRPVKYAGG
                     PMALVNFLMPAHITALACAMLLYANARLGPVIFAVIVAVASKYLIRVRIGGRPRHVLNP
                     SNFGIAVVLVLFPWVGIAPPYQFTEWVGTPLDLAIPALILAAGTMLNAKLTRKMSLILG
                     WIGVFALQALIRTPIEGTATLSALLAMSGVAFVLFTNYMITDPGTTPVRPRNQFVFGAS
                     TALVYALLVHFHVVYGLFFALVIVCLLRGAALAVMDMRQRAAAQPAPLAEPKSVPRRHA
                     PARGGVLATGEEAK"
     gene            887..6481
                     /gene="espE"
     CDS             887..6481
                     /NRPS_PKS="Domain: PKS_KS(Enediyne-KS) (7-463). E-value:
                     2e-95. Score: 312.0. Matches aSDomain:
                     nrpspksdomains_espE_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (620-843). E-value: 4.1e-55.
                     Score: 179.1. Matches aSDomain:
                     nrpspksdomains_espE_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_KR (1205-1385). E-value: 1.9e-31.
                     Score: 101.3. Matches aSDomain:
                     nrpspksdomains_espE_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_DH (1448-1584). E-value: 9e-19.
                     Score: 60.3. Matches aSDomain:
                     nrpspksdomains_espE_PKS_DH.1"
                     /codon_start=1
                     /gene="espE"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     ene_KS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 247.5; E-value:
                     4.2e-75)"
                     /gene_kind="biosynthetic"
                     /note="enediyne warhead iterative type I polyketide
                     synthase"
                     /product="EspE"
                     /protein_id="AAP92148.1"
                     /sec_met_domain="ene_KS (E-value: 3.8e-235, bitscore:
                     768.2, seeds: 19, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 2.2e-56, bitscore: 179.1,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVPSDAIAVVGLGCRHPDASTPEQLWETVLARRRAFRPLPRERL
                     DLGDYGDPAAGPDATDLRYAAVLDGWSFDRSRYRVPGPAYRVTDLAHWLALDVAGDTLA
                     AAGFAGGGGLDRRRAGVILGNTLTGEFSRAALMRLRWPYVRRNLVSALAARGWGAEEMG
                     GLLREVEESYKEPFAEPNDESLVGGLANAIAGRVCNHFDFRGAGYTVDGACSSSLLAVI
                     TACTYLRDGDLDFALAGGVDLSLDPFELVGFSRLGALAGSAMRVYDARPTGFLPGEGCS
                     MVALMRAGDAVAAGRRPIALIRGWGMSSDGNGGLTRPDVSGQRLALERAYARAGFGPET
                     VALFEGHGTGTEVGDRTELHALAEIHDGRRRPRPAALGSVKANIGHTKAAAGAAGLIKA
                     ALALQRQVIPPTTGCDEPHPLLREPGAPLRVVREAEPWPDAPLRAGVSAMGFGGINTHV
                     VLEAAGARRATARLAARDRLTARRPLDCEVFALSAGTDADLAAVLERVAVRARTMSLAE
                     HADLAAALAERAHGDAPVRAAIVARDPAELSARAERAGEILAGLAGPGFAARPGVYVGR
                     GAPGRVGLLFPGQGAPVRESAGALGVVLPEAGEHFEEGLGADPIDTAVAQPAVFRASTA
                     ALRWLDRLGVTAAAAVGHSLGEITALHWAGALGGPDALHLVTTRGRVMADTSQAGTGMA
                     SLAAPAGAVTELIDDADLVIAADNGAVQVVAGPLTEVDRVVKRARAKGITAGRLRVSHA
                     FHSPAVAAAEPVLARHLTDLAVAAPSGRVYSTVTGRELTAADDVRALLARQLTAPVRFR
                     RALELLAADCDLLVEAGPGHTLTAMAQSITDVPVRTLDAGAESAAALGDATAALFAAGA
                     VSDLTALFAERFHRPFDLWREPEFLANPCESAPAVNLDHGLDHGLGRAATATAAPAPAD
                     AVAQAPPEAESDVPAVVRGLIAEALELPPDVIGDGDRLLGDLHLNSLRVVQLAAEAAER
                     TRRAVPAAPPVFAEATVAAFVQAIEELPAAEGEAGSHGPPEGVREWHRVLVEFSRPVEP
                     EAGTASHAWHVFGGGRLRALVEPRLTARPEGEARPAVVAFLPENPDDGAIDALVEAAHL
                     AVDTEAPFTVVDSGDTASGFVGTIAAEYPSLPVRWVRALEAAPADAVGALLAAPWQGHA
                     EIVVDEEGRAGTPAYRAAAPGAPDGRGRDAAAPPLSAGEVLLVTGGGKGIGFASASFLA
                     RRWGVRLGLIGRSGPDADAEPRDNLSRLREAGVDFCYQAADVTDPESLRRGVAAIAGRL
                     GPVRGVIHASGLNRPARFREIGDAYAEHAAPKHHGLRALLDAAGTDGLRLLLTFGSVIG
                     RFGLAGEAHYALANGRMRELARVLARDLPDCRVANLDWTVWSGVGMGERLKVLDDLARA
                     GVSPVPPDRGAELLARAAEAEPPSHSLVVSGRLPQLGRAGEAAPDGHRFLRRVRAWTPG
                     VELVAEAELSLADDPYLDDHRIDGVAVLPAVCVLEAMAQAASALTGRPVAGVADSRFDR
                     PVVVPEHGTRTLRVCALAREDGDVDVAVRSDETGFAVDHFAGTVTAADAEPPENPAAAA
                     RTPAHTGDDLYGPLFFHGRRFRRLRRYAHLEAAACTAVLDGDRPWRFGDGLPADLLFGG
                     PARNDASIHVLQACVPHRRLLPVGCARFAVHRSGDGGGELTLAAVEREHSGADYTYDVV
                     VRDGSGRPVVSWSGLRLRDVGPVPVTTLHPVLVGPYLQRSLTALPPERDVQVAAEAGEP
                     WAVLVEGEPVACARTSDPAPEPPAHWTGLAGRLGRLLGEPDRHVRARLQTVGACLSGPP
                     GPPGLDVQGVYENGWVVLRAGGADIVSVVLSIEGEAGPTAMAIPVKGAS"
     aSDomain        908..2275
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_espE_PKS_KS.1"
                     /domain_subtypes="Enediyne-KS"
                     /evalue="2.00E-95"
                     /label="espE_PKS_KS.1"
                     /locus_tag="espE"
                     /protein_end="463"
                     /protein_start="7"
                     /score="312.0"
                     /tool="antismash"
                     /translation="IAVVGLGCRHPDASTPEQLWETVLARRRAFRPLPRERLDLGDYGD
                     PAAGPDATDLRYAAVLDGWSFDRSRYRVPGPAYRVTDLAHWLALDVAGDTLAAAGFAGG
                     GGLDRRRAGVILGNTLTGEFSRAALMRLRWPYVRRNLVSALAARGWGAEEMGGLLREVE
                     ESYKEPFAEPNDESLVGGLANAIAGRVCNHFDFRGAGYTVDGACSSSLLAVITACTYLR
                     DGDLDFALAGGVDLSLDPFELVGFSRLGALAGSAMRVYDARPTGFLPGEGCSMVALMRA
                     GDAVAAGRRPIALIRGWGMSSDGNGGLTRPDVSGQRLALERAYARAGFGPETVALFEGH
                     GTGTEVGDRTELHALAEIHDGRRRPRPAALGSVKANIGHTKAAAGAAGLIKAALALQRQ
                     VIPPTTGCDEPHPLLREPGAPLRVVREAEPWPDAPLRAGVSAMGFGGINTHVVLEAA"
     aSModule        908..5638
                     /domains="nrpspksdomains_espE_PKS_KS.1"
                     /domains="nrpspksdomains_espE_PKS_AT.1"
                     /domains="nrpspksdomains_espE_PKS_KR.1"
                     /domains="nrpspksdomains_espE_PKS_DH.1"
                     /incomplete
                     /locus_tags="espE"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       1499..1546
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_espE_0001"
                     /evalue="2.20E-03"
                     /label="PKSI-KS_m3"
                     /locus_tag="espE"
                     /protein_end="220"
                     /protein_start="204"
                     /score="10.8"
                     /tool="antismash"
                     /translation="GAGYTVDGACSSSLLA"
     CDS_motif       2018..2062
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_espE_0002"
                     /evalue="1.40E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="espE"
                     /protein_end="392"
                     /protein_start="377"
                     /score="11.2"
                     /tool="antismash"
                     /translation="GSVKANIGHTKAAAG"
     aSDomain        2747..3415
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_espE_PKS_AT.1"
                     /evalue="4.10E-55"
                     /label="espE_PKS_AT.1"
                     /locus_tag="espE"
                     /protein_end="843"
                     /protein_start="620"
                     /score="179.1"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="DTAVAQPAVFRASTAALRWLDRLGVTAAAAVGHSLGEITALHWAG
                     ALGGPDALHLVTTRGRVMADTSQAGTGMASLAAPAGAVTELIDDADLVIAADNGAVQVV
                     AGPLTEVDRVVKRARAKGITAGRLRVSHAFHSPAVAAAEPVLARHLTDLAVAAPSGRVY
                     STVTGRELTAADDVRALLARQLTAPVRFRRALELLAADCDLLVEAGPGHTLTAMAQSIT
                     D"
     CDS_motif       2816..2938
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_espE_0003"
                     /evalue="1.30E-12"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="espE"
                     /protein_end="684"
                     /protein_start="643"
                     /score="39.7"
                     /tool="antismash"
                     /translation="GVTAAAAVGHSLGEITALHWAGALGGPDALHLVTTRGRVMA"
     aSDomain        4502..5041
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_espE_PKS_KR.1"
                     /evalue="1.90E-31"
                     /label="espE_PKS_KR.1"
                     /locus_tag="espE"
                     /protein_end="1385"
                     /protein_start="1205"
                     /score="101.3"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: (unknown)"
                     /tool="antismash"
                     /translation="VLLVTGGGKGIGFASASFLARRWGVRLGLIGRSGPDADAEPRDNL
                     SRLREAGVDFCYQAADVTDPESLRRGVAAIAGRLGPVRGVIHASGLNRPARFREIGDAY
                     AEHAAPKHHGLRALLDAAGTDGLRLLLTFGSVIGRFGLAGEAHYALANGRMRELARVLA
                     RDLPDCRVANLDWTVWS"
     CDS_motif       4508..4600
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_espE_0004"
                     /evalue="6.90E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="espE"
                     /protein_end="1238"
                     /protein_start="1207"
                     /score="12.3"
                     /tool="antismash"
                     /translation="LVTGGGKGIGFASASFLARRWGVRLGLIGRS"
     CDS_motif       4883..4981
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_espE_0005"
                     /evalue="3.10E-05"
                     /label="PKSI-KR_m4"
                     /locus_tag="espE"
                     /protein_end="1365"
                     /protein_start="1332"
                     /score="16.5"
                     /tool="antismash"
                     /translation="RLLLTFGSVIGRFGLAGEAHYALANGRMRELAR"
     aSDomain        5231..5638
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_espE_PKS_DH.1"
                     /evalue="9.00E-19"
                     /label="espE_PKS_DH.1"
                     /locus_tag="espE"
                     /protein_end="1584"
                     /protein_start="1448"
                     /score="60.3"
                     /tool="antismash"
                     /translation="HRFLRRVRAWTPGVELVAEAELSLADDPYLDDHRIDGVAVLPAVC
                     VLEAMAQAASALTGRPVAGVADSRFDRPVVVPEHGTRTLRVCALAREDGDVDVAVRSDE
                     TGFAVDHFAGTVTAADAEPPENPAAAARTPAH"
     CDS_motif       5315..5392
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_espE_0006"
                     /evalue="3.10E-07"
                     /label="PKSI-DH_m2"
                     /locus_tag="espE"
                     /protein_end="1502"
                     /protein_start="1476"
                     /score="23.0"
                     /tool="antismash"
                     /translation="YLDDHRIDGVAVLPAVCVLEAMAQAA"
     gene            6615..>6689
                     /gene="espE7"
     CDS             6615..>6689
                     /codon_start=1
                     /gene="espE7"
                     /note="putative thioesterase; similar to TEBC"
                     /product="EspE7"
                     /protein_id="AAP92149.1"
                     /transl_table=11
                     /translation="MARMHDDLALVTSSCSCDFFAELTP"
ORIGIN
        1 ttctacaccg gtgcgtcggt ttcgagcagt tctgggcgac ccccctcgcc gcgatggccg
       61 tcgcgtacgt gatggagctg gggctggaga ccctggacgc ctgggcgacg cggcggccgg
      121 tcaagtacgc gggcggcccg atggcgctcg tgaacttcct gatgcccgcc cacatcaccg
      181 cgctggcctg cgcgatgctc ctctacgcca acgcgcggct gggaccggtg atcttcgccg
      241 tcatcgtggc ggtcgcgtcg aagtacctga tcagggtccg gatcggcggg cggccgcggc
      301 acgtgctgaa cccctccaac ttcggcatcg cggtggtcct cgtgctgttc ccctgggtcg
      361 ggatcgcgcc cccgtaccag ttcaccgagt gggtcggcac cccgctcgac ctggcgatcc
      421 ccgcgctcat cctcgccgcg gggacgatgc tgaacgccaa gctcaccagg aagatgtccc
      481 tgatcctcgg ctggatcggc gtgttcgctc tccaggcgct gatccggacc cccatcgagg
      541 gcacggcgac gctcagcgcg ctgctggcga tgagcggcgt ggcgttcgtc ctgttcacca
      601 actacatgat cactgacccg gggacgacgc cggtgcggcc gcgcaaccag ttcgtcttcg
      661 gcgcgtcgac cgccctcgtc tacgcgctcc tggtccactt ccacgtggtg tacggcctgt
      721 tcttcgcgct cgtcatcgtg tgcctgctgc gcggcgccgc cctggcggtc atggacatgc
      781 ggcagcgtgc cgcggcgcag cccgcgcccc tggccgagcc caagtccgtg ccccgccggc
      841 acgcccccgc gcgcgggggc gtgctggcga ccggcgagga ggcgaagtga ccgttccgag
      901 cgacgcgatc gccgtcgtcg gcctcgggtg ccgccatccc gacgcctcca cgccggagca
      961 gttgtgggag acggtcctgg cgcggcggcg cgccttccgg ccgctcccgc gcgagcgcct
     1021 cgacctcggc gactacggcg acccggcggc cggccccgac gcgaccgacc tgcggtacgc
     1081 ggccgtgctc gacggctggt ccttcgaccg ctcgcgctac cgggtgcccg gaccggcgta
     1141 ccgggtgacc gacctcgcgc actggctcgc cctggacgtc gccggcgaca cgctggccgc
     1201 cgccggcttc gccgggggag gcggcctgga ccgccggcgc gccggcgtga tcctcggcaa
     1261 caccctgacg ggcgagttca gccgcgccgc gctgatgcgg ctgcgctggc cctacgtccg
     1321 caggaacctg gtgtccgccc tcgccgcgcg cggctggggg gccgaggaga tgggcggcct
     1381 gctccgggag gtcgaggagt cctacaagga gccgttcgcc gaacccaacg acgagagcct
     1441 cgtcggcggg ctggccaacg ccatcgcggg ccgggtctgc aaccacttcg acttccgcgg
     1501 cgcgggctac acggtcgacg gcgcctgctc gtcgtccctg ctcgccgtca tcacggcctg
     1561 cacctacctg cgcgacggcg atctcgactt cgccctcgcg ggcggcgtcg acctcagcct
     1621 cgacccgttc gagctggtcg ggttctcccg gctcggcgcg ctggccggct cggcgatgcg
     1681 ggtgtacgac gcccggccga ccggcttcct gcccggcgag ggctgctcga tggtcgcgct
     1741 gatgcgcgcc ggggacgccg tcgccgccgg gcggcggccg atcgccctga tcaggggctg
     1801 ggggatgtcc tcggacggga acggcggact gacccggccg gacgtctccg ggcagcggct
     1861 cgccctggaa cgcgcctacg cgcgggcggg cttcggaccg gagaccgtgg cgctgttcga
     1921 gggccacggc accggcaccg aggtcggcga ccgcaccgaa ctgcacgccc tcgccgagat
     1981 ccacgacggg cggcggagac cgcgaccggc ggcgctcgga tccgtcaagg ccaacatcgg
     2041 gcacacgaag gcggccgcgg gcgccgccgg gctgatcaag gcggcgctgg cgctgcagcg
     2101 gcaggtcatc ccgccgacca ccggctgcga cgagccccac ccgctcctgc gcgaaccggg
     2161 cgcgccgctg cgcgtcgtcc gcgaggccga gccctggccg gacgccccgc tccgggccgg
     2221 cgtcagcgcg atggggttcg gcggtatcaa cacccatgtc gtcctggagg cggcgggcgc
     2281 ccgccgcgcc acggcgcggt tggcggcccg ggaccgcctg acggcgcgca ggccgctcga
     2341 ctgcgaggtc ttcgccctct ccgccggcac ggacgccgac ctcgccgccg tgctggagcg
     2401 cgtggccgtg cgggcgagga cgatgtcgct cgccgagcac gccgacctgg ccgccgccct
     2461 ggcggagcgg gcgcacggcg acgcgccggt gcgggcggcc atcgtcgccc gcgaccccgc
     2521 cgagctctcc gcgcgcgcgg aacgggcggg cgagatcctc gcgggcctcg ccggacccgg
     2581 gttcgccgcc cggcccggcg tctacgtcgg ccgcggcgct ccgggccggg tcgggctgct
     2641 gttcccgggg cagggcgccc ccgtccggga gagcgccggc gccctcggcg tggtcctccc
     2701 ggaggcgggg gagcacttcg aggaagggct cggggccgac ccgatcgaca ccgccgtcgc
     2761 ccagcccgcc gtcttccgcg cctccacggc ggcgctgcgc tggctcgacc ggctcggcgt
     2821 cacggcggcc gcggcggtcg ggcacagcct cggcgagatc accgccctgc actgggccgg
     2881 cgcgctgggc ggcccggacg ccctgcacct ggtgaccacg cgcggccgcg tcatggccga
     2941 cacctcccag gcgggaaccg ggatggcctc cctcgccgcg ccggccggcg cggtcaccga
     3001 gctcatcgac gacgccgacc tggtgatcgc cgccgacaac ggcgccgtcc aggtcgtcgc
     3061 cggccccctc accgaggtcg accgcgtcgt caagcgggcc cgcgcgaagg ggatcaccgc
     3121 cggcaggctg agggtcagcc acgccttcca ctccccggcg gtcgccgcgg ccgagccggt
     3181 gctcgcgcgc cacctcaccg acctggcggt ggcggcccct tccggtcgcg tgtactccac
     3241 ggtcaccggg cgggagctga ccgccgccga tgacgtccgc gcgctgctcg cccggcagct
     3301 caccgcgccg gtgcggttcc gccgggcgct ggagctcctc gccgcggact gcgacctgct
     3361 cgtcgaggcg ggccccggcc acaccctcac ggcgatggcc cagtcgatca cggacgtgcc
     3421 ggtccgcacg ctggacgcgg gcgcggagtc ggccgccgcg ctcggcgacg ccaccgccgc
     3481 gctgttcgcc gccggcgccg tctccgatct cacggccctc ttcgccgagc ggttccaccg
     3541 cccgttcgac ctctggcggg agccggagtt cctcgccaac ccctgcgaga gcgccccggc
     3601 cgtcaacctc gatcacggcc tcgaccacgg gctcggccgt gccgcgacgg ccaccgcggc
     3661 cccggccccc gccgacgccg tggcgcaggc gccgcccgag gcggaatccg acgtgcccgc
     3721 cgtggtgcgc gggctcatcg ccgaggccct ggagctcccg cccgatgtga tcggcgacgg
     3781 ggaccggctc ctcggcgacc tccatctcaa ctccctccgc gtcgtccagc tcgccgccga
     3841 ggccgccgaa cggacccggc gggccgtgcc ggccgcgccg ccggtcttcg ccgaggccac
     3901 ggtcgccgcg ttcgtccagg cgatcgagga actgcccgcc gcggaaggcg aggccgggtc
     3961 ccacgggccg ccggaggggg tccgcgaatg gcatcgcgtc ctggtcgagt tcagccgtcc
     4021 ggtcgaaccg gaagccggaa ccgcctccca tgcctggcac gtcttcggcg gcggacggct
     4081 ccgcgcgctc gtcgagccgc ggctgaccgc ccgccccgag ggggaggcgc gcccggcggt
     4141 cgtcgcgttc ctccccgaga acccggacga cggcgcgatc gacgcgctgg tcgaagccgc
     4201 gcacctcgcg gtggacaccg aggcgccctt caccgtcgtg gactcgggcg acaccgcgtc
     4261 gggcttcgtg ggcacgatcg ccgccgagta cccgtccctg ccggtccgct gggtccgggc
     4321 tctggaggcc gcacccgcgg acgcggtcgg cgcactgctc gcggccccgt ggcagggcca
     4381 tgccgagatc gtcgtggacg aggagggccg cgccggaacc cccgcgtacc gggccgccgc
     4441 ccccggcgcg cccgacggcc gggggcggga cgccgccgcg cctccgctgt ccgctggaga
     4501 ggtgctcctg gtgacgggag gcgggaaggg catcggcttc gcgagcgcgt ccttcctggc
     4561 gcgtcgctgg ggggtccggc tggggctgat cggacgcagc gggccggacg ccgacgccga
     4621 gccgcgggac aacctctccc ggctgcggga ggccggcgtc gacttctgct accaggccgc
     4681 ggacgtcacc gatccggagt cgctccgccg cggcgtcgcc gcgatcgccg gccggctcgg
     4741 accggtcagg ggagtgatcc acgccagtgg cctgaaccgc cccgcgcggt tccgcgagat
     4801 cggcgacgcc tacgccgagc acgcggcgcc caagcaccac gggctccgcg cgctgctgga
     4861 cgccgccggc acggacgggc tgcggctgct cctcaccttc ggctccgtga tcggccgttt
     4921 cggcctcgcc ggggaggcgc actacgccct ggccaacggc cgcatgcggg agctggcccg
     4981 ggtcctcgcc cgggacctgc cggactgccg ggtcgccaac ctcgactgga cggtctggtc
     5041 gggagtcggc atgggcgagc gcctcaaggt gctcgacgac ctcgcccgcg cgggcgtgtc
     5101 gccggtgccc cccgaccgcg gcgccgagct gctcgcccgg gcggccgagg cggagccgcc
     5161 ctcgcactcc ctggtggtca gcggccggct gccgcagctc ggccgcgccg gggaggcggc
     5221 cccggacggg caccgcttcc tgcggcgggt gcgcgcgtgg accccgggcg tcgaactggt
     5281 ggccgaggcc gagctgagcc tggccgacga cccgtacctg gacgaccacc gcatcgacgg
     5341 tgtggccgtg ctgccggcgg tgtgcgtgct cgaggcgatg gcccaggcgg cgtccgcgct
     5401 gaccgggcgg cccgtggcgg gggtggcgga ttcccgcttc gaccgcccgg tggtcgtccc
     5461 cgagcacggg acccgcaccc tccgggtgtg cgcgctcgcc cgcgaggacg gagacgtgga
     5521 cgtcgcggtg cgcagtgacg agacgggctt cgcggtcgac cacttcgccg ggaccgtcac
     5581 cgcggcggac gccgagcccc cggagaaccc ggccgcggcg gcgcggacac cggcccacac
     5641 cggcgacgac ctgtacgggc cgctcttctt ccacggccgg cgcttccgca ggctccgccg
     5701 ctacgcgcac ctggaggccg ccgcgtgcac ggccgtgctg gacggcgacc ggccctggcg
     5761 gttcggggac ggcctcccgg cggacctgct gttcggcggc cccgcccgca acgacgcctc
     5821 gatccacgtc ctccaggcgt gcgtccccca ccggcggctt ctgccggtcg ggtgcgcgcg
     5881 gttcgccgtc caccggagcg gggacggagg gggcgagctg acgctggccg ccgtcgaacg
     5941 cgagcactcc ggcgccgact acacctacga cgtggtggtg cgcgacggct ccggccggcc
     6001 ggtcgtgagc tggagcgggc tccgccttcg cgacgtcggc cccgtaccgg tcacgaccct
     6061 gcacccggtg ctcgtcgggc cgtacctgca gcgcagcctc accgccctgc ccccggagcg
     6121 cgacgtccag gtggccgccg aggccggcga gccctgggcc gtcctggtgg agggggagcc
     6181 cgtcgcctgc gcgcggacct ccgatccggc gccggagccc cccgcgcact ggaccggcct
     6241 cgccggccgg ctcggccgcc tcctcggcga gccggaccgg cacgtccggg cgcgactgca
     6301 gacggtcggc gcatgcctgt ccgggccgcc gggcccgccc ggactcgacg tccaaggcgt
     6361 ttacgagaac ggatgggtgg tcctccgggc cgggggagcg gacatcgtct ccgtggtcct
     6421 ttccatcgag ggagaagccg ggccgacggc gatggcgatc cccgtcaagg gggcctcgtg
     6481 agggcgtatt tcgagtaccg gcatctcgtg acgttcgccg acaccaacct cgtggggaac
     6541 gttatttcac gaactatctc tcctggcagg gcgcctgccg ggagcggttt ctcgcggaga
     6601 aggcgccgaa gaccgtggcg cgcatgcacg acgacctcgc gctggtcacc tcgtcgtgct
     6661 cgtgcgactt cttcgccgag ctgacgcca
//
