BGC0000060 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000060 (Streptomyces pulveraceus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	73,577	High	fostriecin	PKS

BGC0000060 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 73,577 nt. (total: 73,577 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 73,578
Label		BGC0000060

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

fosD: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

fosG: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

fosJ: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

fosK: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acylase methyltransferase

AEC13064.1: methyltransferase

smcogs: SMCOG1089: methyltransferase

fosC: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase hydrolase

AEC13083.1: thioesterase

smcogs: SMCOG1004: thioesterase

fosL: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Ligases: synthase

fosA: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

fosB: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

fosE: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

fosF: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000060.5	1.00	PKS	fostriecin	Streptomyces pulveraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000060.5	1.00	PKS	fostriecin	Streptomyces pulveraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 73577:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - redmal) + (ccmal) + (ccmal - ohmal) + (Me-ccmal) + (ccmal - ohmal) + (ohmal)
Non-colinear ordering:: fosA + fosB + fosC + fosD + fosE + fosF

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

fosC: mal - mal

PKS_AT (653..950): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 188.6
inactive: 101.2
Methoxymalonyl-CoA: 74.2
Methylmalonyl-CoA: 72.4
Ethylmalonyl-CoA: 42.7
Propionyl-CoA: 39.9
2-Methylbutyryl-CoA: 38.2
Isobutyryl-CoA: 35.2
Benzoyl-CoA: 28.5
Acetyl-CoA: 27.8
CHC-CoA: 26.4
trans-1,2-CPDA: 25.9
fatty_acid: 16.3
3-Methylbutyryl-CoA: 11.9

PKS_AT (2441..2738): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 187.3
inactive: 94.4
Methoxymalonyl-CoA: 78.1
Methylmalonyl-CoA: 67.3
2-Methylbutyryl-CoA: 42.6
Ethylmalonyl-CoA: 41.9
Propionyl-CoA: 40.7
CHC-CoA: 32.0
Benzoyl-CoA: 27.5
Acetyl-CoA: 26.0
trans-1,2-CPDA: 24.2
Isobutyryl-CoA: 18.9
fatty_acid: 15.5
3-Methylbutyryl-CoA: 11.1

fosD: mmal

PKS_AT (577..874): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 198.1
Ethylmalonyl-CoA: 192.3
Methoxymalonyl-CoA: 156.4
2-Methylbutyryl-CoA: 128.0
trans-1,2-CPDA: 109.2
Propionyl-CoA: 98.7
Malonyl-CoA: 86.8
Isobutyryl-CoA: 76.9
CHC-CoA: 71.1
Benzoyl-CoA: 68.2
Acetyl-CoA: 50.4
3-Methylbutyryl-CoA: 43.8
inactive: 36.7
fatty_acid: 23.1

fosE: mal - mal

PKS_AT (600..890): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 183.9
inactive: 106.2
Methoxymalonyl-CoA: 52.6
Methylmalonyl-CoA: 52.0
Propionyl-CoA: 38.7
fatty_acid: 26.1
Isobutyryl-CoA: 17.4
Ethylmalonyl-CoA: 16.1
Acetyl-CoA: 15.7
Benzoyl-CoA: 14.4
CHC-CoA: 5.8
2-Methylbutyryl-CoA: 4.0
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2450..2740): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 183.9
inactive: 106.2
Methoxymalonyl-CoA: 52.6
Methylmalonyl-CoA: 52.0
Propionyl-CoA: 38.7
fatty_acid: 26.1
Isobutyryl-CoA: 17.4
Ethylmalonyl-CoA: 16.1
Acetyl-CoA: 15.7
Benzoyl-CoA: 14.4
CHC-CoA: 5.8
2-Methylbutyryl-CoA: 4.0
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

fosF: mal

PKS_AT (570..874): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 202.9
inactive: 107.6
Methoxymalonyl-CoA: 63.2
Methylmalonyl-CoA: 62.6
Propionyl-CoA: 48.6
Acetyl-CoA: 29.9
Ethylmalonyl-CoA: 28.3
fatty_acid: 26.8
Isobutyryl-CoA: 18.6
Benzoyl-CoA: 11.9
2-Methylbutyryl-CoA: 11.7
CHC-CoA: 11.2
trans-1,2-CPDA: 4.7
3-Methylbutyryl-CoA: 0.0

fosA: mmal - mal

PKS_AT (550..765): mmal

ATSignature: (unknown)

No matches above 50%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 54.6
Ethylmalonyl-CoA: 49.2
Methoxymalonyl-CoA: 49.1
2-Methylbutyryl-CoA: 35.5
CHC-CoA: 33.6
Propionyl-CoA: 33.2
Malonyl-CoA: 31.7
trans-1,2-CPDA: 29.8
Benzoyl-CoA: 19.2
Isobutyryl-CoA: 15.4
Acetyl-CoA: 15.2
fatty_acid: 8.8
3-Methylbutyryl-CoA: 8.1
inactive: 0.0

PKS_AT (1632..1930): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 172.3
inactive: 77.8
Methoxymalonyl-CoA: 69.0
Methylmalonyl-CoA: 57.3
Acetyl-CoA: 32.7
Propionyl-CoA: 28.4
Isobutyryl-CoA: 27.7
Ethylmalonyl-CoA: 20.6
trans-1,2-CPDA: 14.8
fatty_acid: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

fosB: mal

PKS_AT (567..868): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 173.1
inactive: 75.2
Methoxymalonyl-CoA: 65.8
Methylmalonyl-CoA: 59.6
Propionyl-CoA: 31.0
Isobutyryl-CoA: 29.8
Acetyl-CoA: 29.6
Ethylmalonyl-CoA: 22.0
trans-1,2-CPDA: 13.9
CHC-CoA: 7.0
fatty_acid: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor