BGC0000063 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000063 (Fusarium oxysporum)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	53,327	High	hydroxyl-Fumonisin C1/Fumonisin C1/Fumonisin C2/Fumonisin C3/Fumonisin B1	PKS

BGC0000063 - Region 1 - T1PKS

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Location: 1 - 53,327 nt. (total: 53,327 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 53,328
Label		BGC0000063

Tailoring functions

Oxidoreductases: oxygenase

cpm1: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

fum15: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

fum2: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

fum6: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Lyases: dehydratase

fum13: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

fum1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

fum10: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

fum14: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

fum16: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

fum8: 8-amino-7-oxononanoate synthase

smcogs: SMCOG1109: 8-amino-7-oxononanoate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000063.3	0.91	PKS	hydroxyl-Fumonisin C1, Fumonisin C1, Fumonisin C2, Fumonisin C3, Fumonisin B1	Fusarium oxysporum
BGC0000062.4	0.81	PKS	Fumonisin B1, Fumonisin B2, Fumonisin B3	Fusarium verticillioides
BGC0002502.3	0.79	PKS	fumonisin B3, fumonisin B2, fumonisin B1	Fusarium fujikuroi
BGC0002191.2	0.75	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.65	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.65	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001254.3	0.63	PKS	ACT-Toxin II	Alternaria alternata
BGC0002192.3	0.63	PKS	FR901512	fungal sp. No.14919
BGC0001268.4	0.62	NRPS, PKS	fusarin C	Fusarium fujikuroi

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000063.3	0.99	PKS	hydroxyl-Fumonisin C1, Fumonisin C1, Fumonisin C2, Fumonisin C3, Fumonisin B1	Fusarium oxysporum
BGC0000062.4	0.86	PKS	Fumonisin B1, Fumonisin B2, Fumonisin B3	Fusarium verticillioides
BGC0002502.3	0.84	PKS	fumonisin B3, fumonisin B2, fumonisin B1	Fusarium fujikuroi
BGC0002191.2	0.75	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002192.3	0.66	PKS	FR901512	fungal sp. No.14919
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.65	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.65	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001268.4	0.64	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001254.3	0.63	PKS	ACT-Toxin II	Alternaria alternata

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 35569:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

fum1: pk

PKS_AT (608..914): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 70.8%; Methoxymalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 93.5
Methoxymalonyl-CoA: 82.2
Ethylmalonyl-CoA: 58.9
Malonyl-CoA: 58.0
Isobutyryl-CoA: 51.1
Propionyl-CoA: 43.9
trans-1,2-CPDA: 39.3
3-Methylbutyryl-CoA: 30.7
Benzoyl-CoA: 25.8
2-Methylbutyryl-CoA: 24.3
inactive: 24.1
fatty_acid: 22.5
CHC-CoA: 18.9
Acetyl-CoA: 10.0

fum10: X

AMP-binding (30..459): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IIAHRGVPAK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

fum16: X

AMP-binding (83..542): X

nrpys: (unknown)

SVM prediction details:

NOTE: uncertain match

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: EMEVGV--FK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor