BGC0000066 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000066 (Streptomyces hygroscopicus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS-like	1	69,644	High	geldanamycin	PKS

BGC0000066 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 69,644 nt. (total: 69,644 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 69,645
Label		BGC0000066

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

gdmI: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

gdmK: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

gdmL: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

gdmM: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

gdmP: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acetyltransferase

gdmAII: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

Hydrolases: hydrolase phosphatase

AAO06911.1: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

AAO06931.1: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

gdmH: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

Ligases: synthase

gdmAI: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

gdmAIII: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

gdmO: 3-dehydroquinate synthase

smcogs: SMCOG1183: 3-dehydroquinate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000066.5	1.52	PKS	geldanamycin	Streptomyces hygroscopicus
BGC0000067.5	1.23	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000074.5	0.92	PKS	herbimycin A	Streptomyces hygroscopicus
BGC0001273.3	0.90	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.89	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.89	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000068.5	0.86	PKS	geldanamycin	Streptomyces hygroscopicus
BGC0000041.3	0.78	PKS	oronofacic acid	Pseudomonas syringae
BGC0002191.2	0.65	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001276.3	0.65	PKS	6-methylsalicyclic acid	Aspergillus terreus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000066.5	1.00	PKS	geldanamycin	Streptomyces hygroscopicus
BGC0000067.5	0.95	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000068.5	0.89	PKS	geldanamycin	Streptomyces hygroscopicus
BGC0000074.5	0.81	PKS	herbimycin A	Streptomyces hygroscopicus
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.71	PKS	oronofacic acid	Pseudomonas syringae
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 69644:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (AHBA - Me-redmal - redmxmal - Me-ohmal) + (Me-ccmal - ohmxmal) + (redmal - Me-ccmal)
Non-colinear ordering:: gdmAI + gdmAII + gdmAIII

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

gdmAI: AHBA - mmal - mxmal - mmal

CAL_domain (34..442): AHBA

minowa: AHBA

Prediction, score:

AHBA: 538.5
shikimic_acid: 267.8
Acetyl-CoA: 12.5
fatty_acid: 0.0
NH2: 0.0

PKS_AT (1643..1941): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 202.5
Ethylmalonyl-CoA: 186.6
Methoxymalonyl-CoA: 169.2
2-Methylbutyryl-CoA: 123.1
Propionyl-CoA: 108.6
Malonyl-CoA: 100.1
trans-1,2-CPDA: 96.1
CHC-CoA: 72.7
Isobutyryl-CoA: 69.0
Benzoyl-CoA: 58.0
3-Methylbutyryl-CoA: 36.9
Acetyl-CoA: 34.3
fatty_acid: 25.3
inactive: 23.5

PKS_AT (3714..4014): mxmal

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 91.7%; Methylmalonyl-CoA: 91.7%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 199.0
Methylmalonyl-CoA: 191.2
Ethylmalonyl-CoA: 186.5
2-Methylbutyryl-CoA: 137.1
trans-1,2-CPDA: 110.3
Propionyl-CoA: 96.6
Malonyl-CoA: 95.8
CHC-CoA: 89.5
Isobutyryl-CoA: 65.5
Benzoyl-CoA: 53.8
Acetyl-CoA: 43.9
3-Methylbutyryl-CoA: 38.1
inactive: 27.6
fatty_acid: 17.3

PKS_AT (5781..6080): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 204.2
Ethylmalonyl-CoA: 186.6
Methoxymalonyl-CoA: 169.7
2-Methylbutyryl-CoA: 120.9
Propionyl-CoA: 108.3
Malonyl-CoA: 98.4
trans-1,2-CPDA: 95.8
CHC-CoA: 75.1
Isobutyryl-CoA: 64.5
Benzoyl-CoA: 63.0
Acetyl-CoA: 43.1
3-Methylbutyryl-CoA: 32.4
fatty_acid: 25.8
inactive: 18.8

gdmAII: mmal - mxmal

PKS_AT (574..871): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 203.9
Ethylmalonyl-CoA: 188.1
Methoxymalonyl-CoA: 170.9
2-Methylbutyryl-CoA: 122.3
Propionyl-CoA: 109.7
Malonyl-CoA: 101.0
trans-1,2-CPDA: 97.7
CHC-CoA: 72.1
Isobutyryl-CoA: 71.3
Benzoyl-CoA: 57.2
Acetyl-CoA: 37.9
3-Methylbutyryl-CoA: 37.3
fatty_acid: 25.1
inactive: 22.8

PKS_AT (2350..2650): mxmal

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 91.7%; Methylmalonyl-CoA: 91.7%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 198.6
Methylmalonyl-CoA: 190.2
Ethylmalonyl-CoA: 185.0
2-Methylbutyryl-CoA: 132.9
trans-1,2-CPDA: 113.4
Propionyl-CoA: 97.1
Malonyl-CoA: 94.2
CHC-CoA: 94.2
Isobutyryl-CoA: 62.0
Benzoyl-CoA: 54.8
Acetyl-CoA: 44.3
3-Methylbutyryl-CoA: 38.2
inactive: 27.6
fatty_acid: 23.9

gdmAIII: mal - mmal

PKS_AT (583..885): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 231.5
inactive: 122.6
Methoxymalonyl-CoA: 70.3
Methylmalonyl-CoA: 62.7
Propionyl-CoA: 40.8
Isobutyryl-CoA: 32.2
Benzoyl-CoA: 29.7
Ethylmalonyl-CoA: 21.5
Acetyl-CoA: 18.5
trans-1,2-CPDA: 14.6
CHC-CoA: 13.1
fatty_acid: 10.8
2-Methylbutyryl-CoA: 10.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (2663..2962): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 205.6
Ethylmalonyl-CoA: 187.2
Methoxymalonyl-CoA: 169.8
2-Methylbutyryl-CoA: 121.8
Propionyl-CoA: 105.4
Malonyl-CoA: 100.8
trans-1,2-CPDA: 94.2
CHC-CoA: 78.4
Isobutyryl-CoA: 68.1
Benzoyl-CoA: 61.4
Acetyl-CoA: 37.8
3-Methylbutyryl-CoA: 36.9
fatty_acid: 23.4
inactive: 23.3

Link to PARAS substrate predictor