BGC0000067 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000067 (Streptomyces hygroscopicus subsp. duamyceticus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS-like	1	75,920	Low	candicidin	NRPS:Type I+PKS

BGC0000067 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 75,920 nt. (total: 75,920 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 75,921
Label		BGC0000067

Tailoring functions

Transferases: acetyltransferase

ABB86409.1: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

Ligases: synthase

ABB86408.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ABB86410.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000067.5	1.69	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001275.3	0.89	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000066.5	0.85	PKS	geldanamycin	Streptomyces hygroscopicus
BGC0000041.3	0.75	PKS	oronofacic acid	Pseudomonas syringae
BGC0002191.2	0.65	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001276.3	0.65	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000074.5	0.63	PKS	herbimycin A	Streptomyces hygroscopicus
BGC0002192.3	0.61	PKS	FR901512	fungal sp. No.14919
BGC0001160.5	0.58	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001268.4	0.57	NRPS, PKS	fusarin C	Fusarium fujikuroi

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000067.5	1.00	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000066.5	0.78	PKS	geldanamycin	Streptomyces hygroscopicus
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.70	PKS	oronofacic acid	Pseudomonas syringae
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000074.5	0.65	PKS	herbimycin A	Streptomyces hygroscopicus
BGC0002192.3	0.64	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.64	PKS	ACT-Toxin II	Alternaria alternata

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 75920:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (AHBA - Me-redmal - redmxmal - Me-ohmal) + (Me-ccmal - ohmxmal) + (redmal - Me-ccmal)
Non-colinear ordering:: ABB86408.1 + ABB86409.1 + ABB86410.1

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ABB86408.1: AHBA - mmal - mxmal - mmal

CAL_domain (34..442): AHBA

minowa: AHBA

Prediction, score:

AHBA: 538.5
shikimic_acid: 267.8
Acetyl-CoA: 12.5
fatty_acid: 0.0
NH2: 0.0

PKS_AT (1657..1954): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 204.6
Ethylmalonyl-CoA: 188.2
Methoxymalonyl-CoA: 171.6
2-Methylbutyryl-CoA: 124.0
Propionyl-CoA: 109.7
Malonyl-CoA: 102.6
trans-1,2-CPDA: 96.8
CHC-CoA: 73.8
Isobutyryl-CoA: 69.7
Benzoyl-CoA: 58.4
Acetyl-CoA: 36.8
3-Methylbutyryl-CoA: 36.6
fatty_acid: 25.1
inactive: 22.9

PKS_AT (3727..4027): mxmal

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 91.7%; Methylmalonyl-CoA: 91.7%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 196.5
Methylmalonyl-CoA: 186.9
Ethylmalonyl-CoA: 180.8
2-Methylbutyryl-CoA: 134.0
trans-1,2-CPDA: 111.3
Propionyl-CoA: 96.3
Malonyl-CoA: 91.4
CHC-CoA: 87.9
Isobutyryl-CoA: 61.8
Benzoyl-CoA: 52.2
Acetyl-CoA: 42.1
3-Methylbutyryl-CoA: 37.9
inactive: 27.9
fatty_acid: 17.2

PKS_AT (5794..6093): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 206.8
Ethylmalonyl-CoA: 187.4
Methoxymalonyl-CoA: 170.4
2-Methylbutyryl-CoA: 125.5
Propionyl-CoA: 106.0
Malonyl-CoA: 100.8
trans-1,2-CPDA: 94.6
CHC-CoA: 79.2
Isobutyryl-CoA: 67.5
Benzoyl-CoA: 61.2
Acetyl-CoA: 37.0
3-Methylbutyryl-CoA: 36.4
inactive: 24.0
fatty_acid: 23.4

ABB86409.1: mmal - mxmal

PKS_AT (574..871): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 202.5
Ethylmalonyl-CoA: 186.6
Methoxymalonyl-CoA: 169.2
2-Methylbutyryl-CoA: 123.1
Propionyl-CoA: 108.6
Malonyl-CoA: 100.1
trans-1,2-CPDA: 96.1
CHC-CoA: 72.7
Isobutyryl-CoA: 69.0
Benzoyl-CoA: 58.0
3-Methylbutyryl-CoA: 36.9
Acetyl-CoA: 34.3
fatty_acid: 25.3
inactive: 23.5

PKS_AT (2350..2650): mxmal

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 91.7%; Methylmalonyl-CoA: 91.7%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 198.1
Methylmalonyl-CoA: 189.5
Ethylmalonyl-CoA: 184.4
2-Methylbutyryl-CoA: 134.6
trans-1,2-CPDA: 110.3
Propionyl-CoA: 96.8
Malonyl-CoA: 94.7
CHC-CoA: 90.0
Isobutyryl-CoA: 63.6
Benzoyl-CoA: 54.2
Acetyl-CoA: 44.2
3-Methylbutyryl-CoA: 37.9
inactive: 27.4
fatty_acid: 17.3

ABB86410.1: mal - mmal

PKS_AT (583..885): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 231.5
inactive: 122.6
Methoxymalonyl-CoA: 70.3
Methylmalonyl-CoA: 62.7
Propionyl-CoA: 40.8
Isobutyryl-CoA: 32.2
Benzoyl-CoA: 29.7
Ethylmalonyl-CoA: 21.5
Acetyl-CoA: 18.5
trans-1,2-CPDA: 14.6
CHC-CoA: 13.1
fatty_acid: 10.8
2-Methylbutyryl-CoA: 10.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (2663..2962): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 205.5
Ethylmalonyl-CoA: 189.0
Methoxymalonyl-CoA: 170.4
2-Methylbutyryl-CoA: 120.6
Propionyl-CoA: 106.5
Malonyl-CoA: 100.8
trans-1,2-CPDA: 95.7
CHC-CoA: 74.9
Isobutyryl-CoA: 67.2
Benzoyl-CoA: 62.8
Acetyl-CoA: 43.3
3-Methylbutyryl-CoA: 33.7
fatty_acid: 24.0
inactive: 18.7

Link to PARAS substrate predictor