BGC0000079 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000079 (Streptomyces antibioticus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	79,604	High	X-14547	PKS

BGC0000079 - Region 1 - T1PKS

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Location: 1 - 79,604 nt. (total: 79,604 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 79,605
Label		BGC0000079

Tailoring functions

Oxidoreductases: dehydrogenase

idmE: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

idmF: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

idmI: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

idmP: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: transferase

idmB: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

Hydrolases: esterase

idmA: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

idmJ: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

idmL: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

idmM: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

idmN: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

idmO: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000079.5	1.00	PKS	X-14547	Streptomyces antibioticus
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000067.5	0.69	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000018.5	0.68	PKS	angolamycin	Streptomyces eurythermus
BGC0000113.5	0.66	PKS	niddamycin	Streptomyces caelestis
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000079.5	1.00	PKS	X-14547	Streptomyces antibioticus
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000067.5	0.69	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000018.5	0.68	PKS	angolamycin	Streptomyces eurythermus
BGC0000113.5	0.66	PKS	niddamycin	Streptomyces caelestis
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 79604:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - ohmal - pk) + (ccmal - ccmal) + (Me-ohmal) + (ccmal - pk - Me-ohmal) + (redmal)
Non-colinear ordering:: idmL + idmM + idmP + idmN + idmO

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

idmJ: Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (9..394): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLLYLALVCK
Nearest Stachelhaus code(s):: Pro DLLYLALVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

idmL: mal - mal - pk

PKS_AT (569..865): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 70.8%; CE-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 140.2
Methylmalonyl-CoA: 124.6
Methoxymalonyl-CoA: 101.7
Ethylmalonyl-CoA: 82.0
2-Methylbutyryl-CoA: 75.7
Isobutyryl-CoA: 75.5
Propionyl-CoA: 65.4
Benzoyl-CoA: 58.0
trans-1,2-CPDA: 47.1
inactive: 45.7
Acetyl-CoA: 45.4
fatty_acid: 26.1
CHC-CoA: 24.1
3-Methylbutyryl-CoA: 19.8

PKS_AT (1617..1918): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 231.6
inactive: 124.4
Methoxymalonyl-CoA: 77.6
Methylmalonyl-CoA: 67.9
Propionyl-CoA: 46.4
Isobutyryl-CoA: 40.8
2-Methylbutyryl-CoA: 39.0
Ethylmalonyl-CoA: 34.6
CHC-CoA: 29.2
Benzoyl-CoA: 24.7
Acetyl-CoA: 19.5
fatty_acid: 16.4
trans-1,2-CPDA: 9.8
3-Methylbutyryl-CoA: 0.0

PKS_AT (3180..3478): pk

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%; Methylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 179.3
Ethylmalonyl-CoA: 173.3
Methoxymalonyl-CoA: 141.6
2-Methylbutyryl-CoA: 107.7
trans-1,2-CPDA: 96.6
Propionyl-CoA: 93.0
Malonyl-CoA: 83.1
Isobutyryl-CoA: 80.4
CHC-CoA: 76.3
Benzoyl-CoA: 42.6
fatty_acid: 38.2
Acetyl-CoA: 37.1
3-Methylbutyryl-CoA: 36.5
inactive: 24.2

idmM: mal - mal

PKS_AT (563..848): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.2
inactive: 110.6
Methoxymalonyl-CoA: 74.5
Methylmalonyl-CoA: 69.5
Propionyl-CoA: 49.0
Isobutyryl-CoA: 43.7
Ethylmalonyl-CoA: 35.6
Benzoyl-CoA: 29.4
2-Methylbutyryl-CoA: 29.0
CHC-CoA: 23.5
trans-1,2-CPDA: 19.8
Acetyl-CoA: 17.2
fatty_acid: 14.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (2296..2581): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 200.2
inactive: 109.0
Methoxymalonyl-CoA: 71.8
Methylmalonyl-CoA: 64.5
Propionyl-CoA: 38.4
Isobutyryl-CoA: 35.7
Ethylmalonyl-CoA: 27.9
Benzoyl-CoA: 24.8
CHC-CoA: 23.8
Acetyl-CoA: 18.1
trans-1,2-CPDA: 15.5
2-Methylbutyryl-CoA: 14.8
fatty_acid: 10.6
3-Methylbutyryl-CoA: 3.3

idmN: mal - pk - mmal

PKS_AT (570..855): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.2
inactive: 111.2
Methoxymalonyl-CoA: 76.8
Methylmalonyl-CoA: 71.5
Propionyl-CoA: 45.9
Isobutyryl-CoA: 44.3
Ethylmalonyl-CoA: 36.9
Benzoyl-CoA: 28.0
CHC-CoA: 27.6
2-Methylbutyryl-CoA: 24.2
trans-1,2-CPDA: 20.3
Acetyl-CoA: 18.8
fatty_acid: 16.8
3-Methylbutyryl-CoA: 0.0

PKS_AT (2397..2695): pk

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%; Methylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 179.3
Ethylmalonyl-CoA: 173.3
Methoxymalonyl-CoA: 141.6
2-Methylbutyryl-CoA: 107.7
trans-1,2-CPDA: 96.6
Propionyl-CoA: 93.0
Malonyl-CoA: 83.1
Isobutyryl-CoA: 80.4
CHC-CoA: 76.3
Benzoyl-CoA: 42.6
fatty_acid: 38.2
Acetyl-CoA: 37.1
3-Methylbutyryl-CoA: 36.5
inactive: 24.2

PKS_AT (4129..4427): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 217.1
Ethylmalonyl-CoA: 208.2
Methoxymalonyl-CoA: 172.1
2-Methylbutyryl-CoA: 144.3
trans-1,2-CPDA: 132.6
Propionyl-CoA: 115.2
Malonyl-CoA: 103.6
CHC-CoA: 90.8
Isobutyryl-CoA: 73.4
Benzoyl-CoA: 72.6
Acetyl-CoA: 50.4
fatty_acid: 39.7
3-Methylbutyryl-CoA: 33.7
inactive: 32.2

idmO: mal

PKS_AT (572..857): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 205.7
inactive: 111.7
Methoxymalonyl-CoA: 73.7
Methylmalonyl-CoA: 65.5
Propionyl-CoA: 43.3
Isobutyryl-CoA: 35.6
Benzoyl-CoA: 27.7
Ethylmalonyl-CoA: 26.9
2-Methylbutyryl-CoA: 20.2
CHC-CoA: 19.8
Acetyl-CoA: 16.8
trans-1,2-CPDA: 15.3
fatty_acid: 11.7
3-Methylbutyryl-CoA: 0.0

idmP: mmal

PKS_AT (582..880): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 217.1
Ethylmalonyl-CoA: 208.2
Methoxymalonyl-CoA: 172.1
2-Methylbutyryl-CoA: 144.3
trans-1,2-CPDA: 132.6
Propionyl-CoA: 115.2
Malonyl-CoA: 103.6
CHC-CoA: 90.8
Isobutyryl-CoA: 73.4
Benzoyl-CoA: 72.6
Acetyl-CoA: 50.4
fatty_acid: 39.7
3-Methylbutyryl-CoA: 33.7
inactive: 32.2

Link to PARAS substrate predictor