BGC0000083: lactimidomycin biosynthetic gene cluster from Streptomyces amphibiosporus
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Location: 1 - 50,543 nt. (total: 50,543 nt).
This entry is originally from NCBI GenBank GQ274954.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
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resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000083
Short description lactimidomycin biosynthetic gene cluster from Streptomyces amphibiosporus
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • PKS (Type I)
Loci
GQ274954.1
via Knock-out studies
Compounds
  • lactimidomycin
  • 8,9-dihydrolactimidomycin
  • 8-hydroxy-8,9-dihydrolactidomycin
  • 17-hydroxy-8-desmethoxy-isomigrastatin
Species Streptomyces amphibiosporus [taxonomy]
References
Chemical products information
lactimidomycin Evidence:
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C26H35N1O6
Chemical database entries
NPAtlas
PubCHEM
8,9-dihydrolactimidomycin Evidence:
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C26H37N1O6
Chemical database entries
NPAtlas
8-hydroxy-8,9-dihydrolactidomycin Evidence:
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C26H37N1O7
Chemical database entries
NPAtlas
17-hydroxy-8-desmethoxy-isomigrastatin Evidence:
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C26H37N1O7
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • ACY01397.1
 1 - 1698 (+) trans-acting acyl transferase/thioesterase fusion protein
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    • ACY01398.1
    		 1908 - 2162 (+) acyl carrier protein
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      • ACY01399.1
      		 2166 - 4136 (+) amidotransferase
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        • ACY01400.1
        		 4152 - 14462 (+) AT-less polyketide synthase
        • Scaffold biosynthesis
        • Knock-out
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        • ACY01401.1
        		 14549 - 39631 (+) AT-less polyketide synthase
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          • ACY01402.1
          		 39628 - 45924 (+) AT-less polyketide synthase
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            • ACY01403.1
            		 45926 - 48232 (+) trans-acting acyl transferase/oxidoreductase fusion protein
            • Scaffold biosynthesis
            • Knock-out
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            • ACY01404.1
            		 48441 - 49697 (+) Cytochrome P450 desaturase
            • Tailoring
            • Knock-out
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            • ACY01405.1
            		 49800 - 50543 (-) phosphopantetheinyl transferase
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              Biosynthesis information

              Biosynthetic modules

              Name
              0
              Type
              pks-trans-at-starter
              Genes
              ACY01398.1
              Substrates
              Integrated Monomers
              Domains
              carrier (ACP)
              Name
              1
              Type
              pks-trans-at
              Genes
              ACY01400.1
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, dehydratase, ketoreductase, carrier (ACP)
              Name
              2
              Type
              pks-trans-at
              Genes
              ACY01400.1
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, branching, carrier (ACP)
              Name
              3
              Type
              pks-trans-at
              Genes
              ACY01400.1 ACY01401.1
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, ketoreductase, carrier (ACP)
              Name
              4
              Type
              pks-trans-at
              Genes
              ACY01401.1
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, carrier (ACP)
              Name
              5
              Type
              pks-trans-at
              Genes
              ACY01401.1
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, dehydratase, ketoreductase, methyltransferase, carrier (ACP)
              Name
              6
              Type
              pks-trans-at
              Genes
              ACY01401.1
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, ketoreductase, methyltransferase, carrier (ACP)
              Name
              7
              Type
              pks-trans-at
              Genes
              ACY01401.1
              Substrates
              Integrated Monomers
              Domains
              ketosynthase
              Name
              8
              Type
              pks-trans-at
              Genes
              ACY01401.1
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, dehydratase, ketoreductase, carrier (ACP), carrier (ACP)
              Name
              9
              Type
              pks-trans-at
              Genes
              ACY01401.1 ACY01402.1
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, dehydratase, carrier (ACP)
              Name
              10
              Type
              pks-trans-at
              Genes
              ACY01402.1
              Substrates
              Integrated Monomers
              Domains
              ketosynthase, dehydratase, ketoreductase, carrier (ACP)
              Annotation changelog

              Entry version: 4

              Date
              Changes
              Submitters
              Reviewers
              2022-10-07
              Update chemical activity to schema version 2.11
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

              Entry version: 3

              Date
              Changes
              Submitters
              Reviewers
              2022-09-15
              Fixed inconsistent gene labelling
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              2022-09-15
              Removed ketoreductase stereochemistry annotation from modules without ketoreductases
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              2022-09-15
              Merged cross-CDS PKS modules
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              2022-09-15
              Updated bioactivity data
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

              Entry version: 2

              Date
              Changes
              Submitters
              Reviewers
              2019-10-16
              Migrated from v1.4
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              2019-10-16
              Updated compound(s) information (NPAtlas curation)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

              Entry version: 1

              Date
              Changes
              Submitters
              Reviewers
              2015-06-12
              Submitted
              • (ID: HPZ6LL7T2WJIC44FKRYGWFQ6)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              Detailed domain annotation
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