Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000083.1:
Cluster 1Polyketide150543

BGC0000083, lactimidomycin biosynthetic gene cluster from Streptomyces amphibiosporus. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Lactimidomycin
PubChem ID: 11669726
SMILES string: Copy to clipboard
Molecular formula: C26H35NO6
Average molecular mass: 457.567 Da
Molecular activity: Antifungal, Cytotoxic, Inhibitor
Molecular target: ribosome

Class-specific details

Biosynthetic class(es):
Polyketide
Polyketide subclass:
Macrolide (cyclic)
Polyketide synthase subclass:
Modular type I / Trans-AT type I
Starter unit:
Malonyl-CoA
ACY01403
Thioesterase type:
Type I & II
Release / cyclization type:
Macrolactonization

Modular polyketide synthases:
ltmC (ACY01398.1)
Module 0
Scaffold-modifying domain: None

ltmE (ACY01400.1)
Module 1
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 2
Scaffold-modifying domain: Michael branching
Module 3
Scaffold-modifying domain: None

ltmF (ACY01401.1)
Module 3
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 4
Scaffold-modifying domain: None
Module 5
KR stereochemistry: Unknown
Scaffold-modifying domain: Methylation
Module 6
KR stereochemistry: A-group
Scaffold-modifying domain: Methylation
Module 7
Scaffold-modifying domain: None
Module 8
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 9
Scaffold-modifying domain: None

ltmG (ACY01402.1)
Module 10
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 9
Scaffold-modifying domain: None

Gene cluster description

lactimidomycin (BGC0000083). Gene Cluster 1. Biosynthetic class = Polyketide. GenBank GQ274954, positions 1-50543. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Guohui Pan (The Scripps Research Institute)

Literature references

1. Seo JW et al. (2014) Comparative characterization of the lactimidomycin and iso-migrastatin biosynthetic machineries revealing unusual features for acyltransferase-less type I polyketide synthases and providing an opportunity to engineer new analogues. Biochemistry 53(49):7854-65. doi: 10.1021/bi501396v. Epub 2014 Dec
2. Ju J et al. (2007) New lactimidomycin congeners shed insight into lactimidomycin biosynthesis in Streptomyces amphibiosporus. Org Lett 9(25):5183-6. doi: 10.1021/ol702249g. Epub 2007 Nov 13.