BGC0000083: lactimidomycin biosynthetic gene cluster from Streptomyces amphibiosporus
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Location: 1 - 50,543 nt. (total: 50,543 nt).
This entry is originally from NCBI GenBank GQ274954.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000083
Short description lactimidomycin biosynthetic gene cluster from Streptomyces amphibiosporus
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • Polyketide (Macrolide)
Loci NCBI GenBank: GQ274954.1
Compounds
  • lactimidomycin
  • 8,9-dihydrolactimidomycin
  • 8-hydroxy-8,9-dihydrolactidomycin
  • 17-hydroxy-8-desmethoxy-isomigrastatin
Species Streptomyces amphibiosporus [taxonomy]
References
Chemical products information
lactimidomycin [synonyms: ltm]
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C26H35N1O6
Chemical database entries
NPAtlas
PubCHEM
8,9-dihydrolactimidomycin
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C26H37N1O6
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NPAtlas
8-hydroxy-8,9-dihydrolactidomycin
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C26H37N1O7
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NPAtlas
17-hydroxy-8-desmethoxy-isomigrastatin
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C26H37N1O7
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • ACY01397.1
 1 - 1698 (+) trans-acting acyl transferase/thioesterase fusion protein
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  • ACY01398.1
 1908 - 2162 (+) acyl carrier protein
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  • ACY01399.1
 2166 - 4136 (+) amidotransferase
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  • ACY01400.1
 4152 - 14462 (+) AT-less polyketide synthase
  • Scaffold biosynthesis
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  • ACY01401.1
 14549 - 39631 (+) AT-less polyketide synthase
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  • ACY01402.1
 39628 - 45924 (+) AT-less polyketide synthase
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  • ACY01403.1
 45926 - 48232 (+) trans-acting acyl transferase/oxidoreductase fusion protein
  • Scaffold biosynthesis
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  • ACY01404.1
 48441 - 49697 (+) Cytochrome P450 desaturase
  • Tailoring (Oxidation)
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  • ACY01405.1
 49800 - 50543 (-) phosphopantetheinyl transferase
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Polyketide-specific information
Subclass Macrolide
Starter unit Malonyl-CoA
Cyclic? yes
Release type
  • Macrolactonization
Polyketide-synthases
Genes Properties Modules
ACY01397.1
+
ACY01398.1
+
ACY01400.1
+
ACY01401.1
+
ACY01402.1
+
ACY01403.1 		Synthase subclass: Modular type I/Trans-AT type I
Trans-acyltansferases: ACY01403.1
Thioesterases:
  • ACY01397.1 (Unknown)
  • ACY01402.1 (Unknown)
Module 0
Genes: ACY01398.1
Core domains: Thiolation (ACP/PCP)
Module 1
Genes: ACY01400.1
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 2
Genes: ACY01400.1
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Michael branching
Module 3
Genes: ACY01400.1, ACY01401.1
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 4
Genes: ACY01401.1
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 5
Genes: ACY01401.1
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: Unknown
Module 6
Genes: ACY01401.1
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: D-OH
Module 7
Genes: ACY01401.1
Core domains: Ketosynthase
Module 8
Genes: ACY01401.1
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP), Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 9
Genes: ACY01401.1, ACY01402.1
Core domains: Ketosynthase, Dehydratase, Thiolation (ACP/PCP)
Module 10
Genes: ACY01402.1
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: HPZ6LL7T2WJIC44FKRYGWFQ6, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Fixed inconsistent gene labelling
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Merged cross-CDS PKS modules
  • Updated bioactivity data
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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