BGC0000085 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000085 (Streptomyces rochei)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	76,907	High	lankamycin	PKS

BGC0000085 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 76,907 nt. (total: 76,907 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 76,908
Label		BGC0000085

Tailoring functions

Oxidoreductases: oxidoreductase oxygenase

lkmBII: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

lkmCII: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

lkmCVI: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

lkmF: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

lkmK: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acetyltransferase acyltransferase aminotransferase glycosyltransferase kinase methyltransferase transferase

lkmAI: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

lkmCIV: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

lkmG: methyltransferase

smcogs: SMCOG1089: methyltransferase

lkmI: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

lkmJ: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

lkmL: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

lkmM: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

lkmS: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

lkmU: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

Hydrolases: esterase

lkmE: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

lkmBIV: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

lkmD: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

lkmAII: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

lkmAIII: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000085.5	0.99	PKS	lankamycin	Streptomyces rochei
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001817.5	0.62	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0000067.5	0.62	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001051.5	0.59	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0001065.5	0.57	PKS	herboxidiene	Streptomyces chromofuscus
BGC0000041.3	0.57	PKS	oronofacic acid	Pseudomonas syringae
BGC0000094.5	0.56	PKS, saccharide	methymycin	Streptomyces venezuelae
BGC0001012.3	0.56	NRPS, PKS	meridamycin	Streptomyces violaceusniger

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000085.5	1.00	PKS	lankamycin	Streptomyces rochei
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000067.5	0.62	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001817.5	0.61	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0001051.5	0.59	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0000278.3	0.57	PKS	urdamycin	Streptomyces fradiae
BGC0001065.5	0.57	PKS	herboxidiene	Streptomyces chromofuscus
BGC0000041.3	0.57	PKS	oronofacic acid	Pseudomonas syringae
BGC0000094.5	0.56	PKS, saccharide	methymycin	Streptomyces venezuelae

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 71823:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - Me-ccmal - Me-ohmal) + (Me-ohmal - Me-redmal) + (Me-ohmal - Me-ohmal)
Non-colinear ordering:: lkmAI + lkmAII + lkmAIII

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

lkmAIII: mmal - mmal

PKS_AT (576..870): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 205.0
Ethylmalonyl-CoA: 184.6
Methoxymalonyl-CoA: 168.3
2-Methylbutyryl-CoA: 142.4
Propionyl-CoA: 110.4
trans-1,2-CPDA: 108.7
CHC-CoA: 106.3
Malonyl-CoA: 98.0
Isobutyryl-CoA: 69.2
Benzoyl-CoA: 61.8
Acetyl-CoA: 44.0
fatty_acid: 40.3
inactive: 28.3
3-Methylbutyryl-CoA: 26.0

PKS_AT (2079..2375): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.6
Ethylmalonyl-CoA: 206.0
Methoxymalonyl-CoA: 178.0
2-Methylbutyryl-CoA: 160.7
trans-1,2-CPDA: 124.9
Malonyl-CoA: 122.2
Propionyl-CoA: 119.9
CHC-CoA: 103.0
Isobutyryl-CoA: 89.6
3-Methylbutyryl-CoA: 51.3
Benzoyl-CoA: 47.4
Acetyl-CoA: 41.2
inactive: 36.3
fatty_acid: 22.9

lkmAII: mmal - mmal

PKS_AT (562..862): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 209.2
Ethylmalonyl-CoA: 200.0
Methoxymalonyl-CoA: 178.0
2-Methylbutyryl-CoA: 150.8
trans-1,2-CPDA: 126.4
Propionyl-CoA: 118.6
Malonyl-CoA: 115.9
CHC-CoA: 102.2
Isobutyryl-CoA: 101.7
Benzoyl-CoA: 65.1
Acetyl-CoA: 60.4
inactive: 48.2
fatty_acid: 37.0
3-Methylbutyryl-CoA: 30.1

PKS_AT (2063..2360): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 209.9
Ethylmalonyl-CoA: 194.2
Methoxymalonyl-CoA: 168.6
2-Methylbutyryl-CoA: 145.2
Propionyl-CoA: 111.7
trans-1,2-CPDA: 104.1
Malonyl-CoA: 90.5
Isobutyryl-CoA: 89.5
CHC-CoA: 82.8
Benzoyl-CoA: 62.8
3-Methylbutyryl-CoA: 61.4
Acetyl-CoA: 52.5
inactive: 34.9
fatty_acid: 33.2

lkmAI: pk - mmal - mmal

PKS_AT (108..405): pk

ATSignature: 2-Methylbutyryl-CoA

Top 3 matches:: 2-Methylbutyryl-CoA: 100.0%; Propionyl-CoA: 79.2%; Methylmalonyl-CoA: 70.8%

minowa: 2-Methylbutyryl-CoA

Prediction, score:

2-Methylbutyryl-CoA: 257.4
Propionyl-CoA: 151.9
Methylmalonyl-CoA: 135.3
Ethylmalonyl-CoA: 108.1
Methoxymalonyl-CoA: 104.8
CHC-CoA: 80.6
Malonyl-CoA: 75.2
trans-1,2-CPDA: 71.5
Isobutyryl-CoA: 41.7
Acetyl-CoA: 33.5
Benzoyl-CoA: 33.1
fatty_acid: 26.7
inactive: 25.4
3-Methylbutyryl-CoA: 21.6

PKS_AT (1126..1432): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 211.7
Ethylmalonyl-CoA: 208.6
Methoxymalonyl-CoA: 172.0
2-Methylbutyryl-CoA: 133.3
trans-1,2-CPDA: 124.0
Propionyl-CoA: 112.4
Malonyl-CoA: 105.6
CHC-CoA: 97.5
Isobutyryl-CoA: 73.5
fatty_acid: 61.7
Benzoyl-CoA: 59.6
Acetyl-CoA: 44.9
3-Methylbutyryl-CoA: 27.8
inactive: 20.6

PKS_AT (2630..2922): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 202.5
Ethylmalonyl-CoA: 188.2
Methoxymalonyl-CoA: 162.0
2-Methylbutyryl-CoA: 133.8
trans-1,2-CPDA: 115.2
CHC-CoA: 102.8
Propionyl-CoA: 95.8
Malonyl-CoA: 94.5
Isobutyryl-CoA: 73.9
Benzoyl-CoA: 43.9
3-Methylbutyryl-CoA: 41.9
Acetyl-CoA: 37.5
inactive: 21.2
fatty_acid: 18.6

Link to PARAS substrate predictor