BGC0000087 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000087 (Streptomyces lasalocidi)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	110,927	High	lasalocid	PKS

BGC0000087 - Region 1 - T1PKS

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Location: 1 - 110,927 nt. (total: 110,927 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 110,928
Label		BGC0000087

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

CAQ64702.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

CAQ64715.1: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

las5: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

las6: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

lasC: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

Transferases: acylase

lasAIII: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

lasAV: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

lasAVII: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase hydrolase

CAQ64696.1: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

CAQ64709.1: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

las1: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: synthase

lasAI: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

lasAII: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

lasAIV: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

lasAVI: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

pseudo9093_9519: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000087.5	0.97	PKS	lasalocid	Streptomyces lasaliensis
BGC0000086.5	0.88	PKS	lasalocid	Streptomyces lasaliensis
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000018.5	0.72	PKS	angolamycin	Streptomyces eurythermus
BGC0001648.5	0.68	PKS	lasalocid	Streptomyces sp.
BGC0000041.3	0.67	PKS	oronofacic acid	Pseudomonas syringae
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000087.5	1.00	PKS	lasalocid	Streptomyces lasaliensis
BGC0000086.5	0.88	PKS	lasalocid	Streptomyces lasaliensis
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000018.5	0.72	PKS	angolamycin	Streptomyces eurythermus
BGC0001648.5	0.68	PKS	lasalocid	Streptomyces sp.
BGC0000041.3	0.67	PKS	oronofacic acid	Pseudomonas syringae
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 100106:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - pk - redmal) + (pk - Me-redmal - pk) + (Me-mal) + (Me-ohmal) + (redmal - ohmal) + (Me-ccmal) + (mal)
Non-colinear ordering:: lasAI + lasAII + lasAIII + lasAIV + lasAV + lasAVI + lasAVII

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

lasAI: mal - pk - mal

PKS_AT (536..835): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 232.3
inactive: 106.5
Methoxymalonyl-CoA: 83.3
Methylmalonyl-CoA: 66.9
Propionyl-CoA: 52.9
Isobutyryl-CoA: 43.2
Ethylmalonyl-CoA: 30.0
2-Methylbutyryl-CoA: 29.3
Benzoyl-CoA: 22.7
fatty_acid: 20.6
Acetyl-CoA: 20.3
CHC-CoA: 14.7
3-Methylbutyryl-CoA: 13.0
trans-1,2-CPDA: 0.0

PKS_AT (1582..1873): pk

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Methylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 185.3
Ethylmalonyl-CoA: 173.6
Methoxymalonyl-CoA: 150.1
2-Methylbutyryl-CoA: 119.3
trans-1,2-CPDA: 115.1
CHC-CoA: 106.5
Malonyl-CoA: 98.8
Propionyl-CoA: 90.6
Isobutyryl-CoA: 67.7
Benzoyl-CoA: 34.3
fatty_acid: 31.4
inactive: 30.3
Acetyl-CoA: 27.2
3-Methylbutyryl-CoA: 24.8

PKS_AT (3385..3671): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 189.8
inactive: 87.2
Methoxymalonyl-CoA: 49.0
Methylmalonyl-CoA: 34.4
Propionyl-CoA: 27.2
Acetyl-CoA: 17.7
fatty_acid: 17.5
Benzoyl-CoA: 14.7
trans-1,2-CPDA: 14.0
Isobutyryl-CoA: 12.3
2-Methylbutyryl-CoA: 9.4
CHC-CoA: 7.1
Ethylmalonyl-CoA: 6.0
3-Methylbutyryl-CoA: 0.0

lasAII: pk - mmal - pk

PKS_AT (566..857): pk

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Methylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 187.0
Ethylmalonyl-CoA: 175.0
Methoxymalonyl-CoA: 152.0
2-Methylbutyryl-CoA: 118.5
trans-1,2-CPDA: 116.8
CHC-CoA: 105.1
Malonyl-CoA: 97.8
Propionyl-CoA: 90.6
Isobutyryl-CoA: 64.8
Benzoyl-CoA: 34.7
fatty_acid: 30.8
inactive: 30.6
Acetyl-CoA: 27.0
3-Methylbutyryl-CoA: 26.9

PKS_AT (2323..2620): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 200.7
Ethylmalonyl-CoA: 186.2
Methoxymalonyl-CoA: 160.7
2-Methylbutyryl-CoA: 133.6
trans-1,2-CPDA: 124.5
CHC-CoA: 97.3
Propionyl-CoA: 95.5
Malonyl-CoA: 80.7
Isobutyryl-CoA: 58.1
Benzoyl-CoA: 45.4
3-Methylbutyryl-CoA: 23.5
Acetyl-CoA: 17.5
fatty_acid: 16.4
inactive: 13.9

PKS_AT (4389..4680): pk

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Methylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 186.2
Ethylmalonyl-CoA: 174.2
Methoxymalonyl-CoA: 151.1
2-Methylbutyryl-CoA: 123.7
trans-1,2-CPDA: 111.6
CHC-CoA: 106.7
Malonyl-CoA: 99.3
Propionyl-CoA: 90.6
Isobutyryl-CoA: 68.0
Benzoyl-CoA: 34.2
inactive: 32.8
fatty_acid: 31.2
Acetyl-CoA: 24.8
3-Methylbutyryl-CoA: 24.5

lasAIII: mmal

PKS_AT (564..861): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 197.1
Ethylmalonyl-CoA: 183.6
Methoxymalonyl-CoA: 156.0
2-Methylbutyryl-CoA: 131.7
trans-1,2-CPDA: 122.6
Propionyl-CoA: 94.1
CHC-CoA: 93.9
Malonyl-CoA: 78.9
Isobutyryl-CoA: 58.2
Benzoyl-CoA: 44.5
3-Methylbutyryl-CoA: 22.0
Acetyl-CoA: 18.9
fatty_acid: 15.4
inactive: 12.3

lasAIV: mmal

PKS_AT (567..864): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 192.4
Ethylmalonyl-CoA: 178.6
Methoxymalonyl-CoA: 152.3
2-Methylbutyryl-CoA: 131.3
trans-1,2-CPDA: 119.9
Propionyl-CoA: 94.2
CHC-CoA: 91.8
Malonyl-CoA: 77.0
Isobutyryl-CoA: 54.5
Benzoyl-CoA: 46.6
Acetyl-CoA: 22.4
3-Methylbutyryl-CoA: 21.6
fatty_acid: 17.1
inactive: 11.4

lasAV: mal - mal

PKS_AT (564..850): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 180.5
inactive: 81.5
Methoxymalonyl-CoA: 43.8
Methylmalonyl-CoA: 29.9
Propionyl-CoA: 22.4
Acetyl-CoA: 15.2
fatty_acid: 12.5
2-Methylbutyryl-CoA: 12.1
Benzoyl-CoA: 11.9
Isobutyryl-CoA: 11.7
trans-1,2-CPDA: 9.8
CHC-CoA: 0.5
Ethylmalonyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2654..2939): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 180.2
inactive: 81.2
Methoxymalonyl-CoA: 42.4
Methylmalonyl-CoA: 30.7
Propionyl-CoA: 23.0
Acetyl-CoA: 15.2
fatty_acid: 12.5
2-Methylbutyryl-CoA: 12.1
CHC-CoA: 12.0
Benzoyl-CoA: 11.9
Isobutyryl-CoA: 11.7
trans-1,2-CPDA: 11.3
Ethylmalonyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

lasAVI: mmal

PKS_AT (578..875): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 196.4
Ethylmalonyl-CoA: 182.0
Methoxymalonyl-CoA: 155.0
2-Methylbutyryl-CoA: 138.1
trans-1,2-CPDA: 121.6
Propionyl-CoA: 96.9
CHC-CoA: 93.7
Malonyl-CoA: 81.3
Isobutyryl-CoA: 55.4
Benzoyl-CoA: 50.2
3-Methylbutyryl-CoA: 25.4
fatty_acid: 21.6
Acetyl-CoA: 18.1
inactive: 14.7

lasAVII: mal

PKS_AT (566..845): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 184.5
inactive: 82.7
Methoxymalonyl-CoA: 43.7
Methylmalonyl-CoA: 30.1
Propionyl-CoA: 23.1
Acetyl-CoA: 14.9
fatty_acid: 13.9
Benzoyl-CoA: 12.1
Isobutyryl-CoA: 12.0
trans-1,2-CPDA: 9.7
2-Methylbutyryl-CoA: 8.9
CHC-CoA: 6.0
Ethylmalonyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor