BGC0000090: macbecin biosynthetic gene cluster from Actinosynnema pretiosum subsp. pretiosum
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Location: 1 - 100,588 nt. (total: 100,588 nt).
This entry is originally from NCBI GenBank EU827593.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000090
Short description macbecin biosynthetic gene cluster from Actinosynnema pretiosum subsp. pretiosum
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • PKS (Unknown)
Loci
EU827593.1
via Knock-out studies, Heterologous expression
Compounds
  • macbecin
  • macbecin II
Species Actinosynnema pretiosum subsp. pretiosum [taxonomy]
References
Chemical products information
macbecin Evidence:
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C30H42N2O8
Chemical database entries
NPAtlas
macbecin II Evidence: NMR, Mass spectrometry
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C30H44N2O8
Chemical database entries
NPAtlas
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Comment Extra
  • ACF35442.1
  • mbcRII
 14925 - 17909 (+) mbcRII
  • Regulation
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  • ACF35443.1
  • mbcO
 18025 - 19074 (-) mbcO
  • Precursor biosynthesis
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  • ACF35444.1
  • mbcB
 19263 - 20066 (-) mbcB
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    • ACF35445.1
    • mbcAI
    		 20330 - 40657 (+) mbcAI
    • Scaffold biosynthesis
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    • ACF35446.1
    • mbcAII
    		 40654 - 50859 (+) mbcAII
    • Scaffold biosynthesis
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    • ACF35447.1
    • mbcAIII
    		 50867 - 62491 (+) mbcAIII
    • Scaffold biosynthesis
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    • ACF35448.1
    • mbcF
    		 62500 - 63276 (+) mbcF
    • Scaffold biosynthesis
    Release and cyclization of PKS bound chain
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    • ACF35449.1
    • mbcM
    		 63281 - 64852 (+) mbcM
    • Tailoring
    • Knock-out
    Bioinformatic analysis plus studies of other post PKS genes confirmed function
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    • ACF35450.1
    • mbcC
    		 64899 - 65696 (-) mbcC
    • Precursor biosynthesis
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    • ACF35451.1
    • mbcD
    		 65693 - 66853 (-) mbcD
    • Precursor biosynthesis
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    • ACF35452.1
    • mbcE
    		 66891 - 68057 (-) mbcE
    • Precursor biosynthesis
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    • ACF35453.1
    • mbcL
    		 68301 - 68732 (+) mbcL
    • Precursor biosynthesis
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    • ACF35454.1
    • mbcQ
    		 68690 - 69661 (-) mbcQ
    • Precursor biosynthesis
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    • ACF35455.1
    • mbcN
    		 70185 - 72194 (-) mbcN
    • Tailoring
    Carbamoyl transferase; homology to gene in related systems
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    • ACF35456.1
    • mbcH
    		 72248 - 73339 (-) mbcH
    • Precursor biosynthesis
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    • ACF35457.1
    • mbcI
    		 73336 - 74493 (-) mbcI
    • Precursor biosynthesis
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    • ACF35458.1
    • mbcJ
    		 74490 - 74765 (-) mbcJ
    • Precursor biosynthesis
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    • ACF35459.1
    • mbcK
    		 74762 - 75628 (-) mbcK
    • Precursor biosynthesis
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    • ACF35460.1
    • mbcG
    		 75881 - 76537 (+) mbcG
    • Precursor biosynthesis
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    • ACF35461.1
    • mbcP
    		 76534 - 77802 (+) mbcP
    • Tailoring
    • Knock-out
    Desaturase; Bioinformatic analysis plus studies of other post-PKS genes confirmed function inc. complementation
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    • ACF35462.1
    • mbcS
    		 77831 - 79054 (+) mbcS
    • Tailoring
    • Knock-out
    Complementation studies used to verify structure
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    • ACF35463.1
    • mbcT
    		 79119 - 79934 (+) mbcT
    • Tailoring
    • Knock-out
    Complementation studies used to verify function and regiochemistry
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    • ACF35464.1
    • mbcU
    		 79931 - 80716 (+) mbcU
    • Tailoring
    • Knock-out
    Complementation studies used to verify function and regiochemistry
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    Biosynthesis information
    Annotation changelog

    Entry version: 3

    Date
    Changes
    Submitters
    Reviewers
    2022-10-07
    Update chemical activity to schema version 2.11
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

    Entry version: 2

    Date
    Changes
    Submitters
    Reviewers
    2019-10-16
    Migrated from v1.4
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    2019-10-16
    Updated compound(s) information (NPAtlas curation)
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

    Entry version: 1

    Date
    Changes
    Submitters
    Reviewers
    2015-06-12
    Submitted
    • (ID: SOJ5J6FNXPWDFJNGJGPS2QRA)
    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
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