BGC0000090: macbecin biosynthetic gene cluster from Actinosynnema pretiosum subsp. pretiosum
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Location: 1 - 100,588 nt. (total: 100,588 nt).
This entry is originally from NCBI GenBank EU827593.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
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resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
History
NRPS/PKS domains
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General information about the BGC
MIBiG accession BGC0000090
Short description macbecin biosynthetic gene cluster from Actinosynnema pretiosum subsp. pretiosum
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • Polyketide (Ansamycin)
Loci NCBI GenBank: EU827593.1
Compounds
  • macbecin
  • macbecin II
Species Actinosynnema pretiosum subsp. pretiosum [taxonomy]
References
Chemical products information
macbecin
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C30H42N2O8
Chemical database entries
NPAtlas
macbecin II
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C30H44N2O8
Chemical database entries
NPAtlas
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Comment Extra
  • ACF35442.1
  • mbcRII
 14925 - 17909 (+) mbcRII
  • Regulation
Sequence-based prediction
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  • ACF35443.1
  • mbcO
 18025 - 19074 (-) mbcO
  • Precursor biosynthesis
Sequence-based prediction
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  • ACF35444.1
  • mbcB
 19263 - 20066 (-) mbcB
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  • ACF35445.1
  • mbcAI
 20330 - 40657 (+) mbcAI
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACF35446.1
  • mbcAII
 40654 - 50859 (+) mbcAII
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACF35447.1
  • mbcAIII
 50867 - 62491 (+) mbcAIII
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACF35448.1
  • mbcF
 62500 - 63276 (+) mbcF
  • Scaffold biosynthesis
Sequence-based prediction
Release and cyclization of PKS bound chain
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  • ACF35449.1
  • mbcM
 63281 - 64852 (+) mbcM
  • Tailoring (Monooxygenation)
Knock-out
Bioinformatic analysis plus studies of other post PKS genes confirmed function
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  • ACF35450.1
  • mbcC
 64899 - 65696 (-) mbcC
  • Precursor biosynthesis
Sequence-based prediction
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  • ACF35451.1
  • mbcD
 65693 - 66853 (-) mbcD
  • Precursor biosynthesis
Sequence-based prediction
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  • ACF35452.1
  • mbcE
 66891 - 68057 (-) mbcE
  • Precursor biosynthesis
Sequence-based prediction
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  • ACF35453.1
  • mbcL
 68301 - 68732 (+) mbcL
  • Precursor biosynthesis
Sequence-based prediction
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  • ACF35454.1
  • mbcQ
 68690 - 69661 (-) mbcQ
  • Precursor biosynthesis
Sequence-based prediction
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  • ACF35455.1
  • mbcN
 70185 - 72194 (-) mbcN
  • Tailoring (Unknown)
Sequence-based prediction
Carbamoyl transferase; homology to gene in related systems
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  • ACF35456.1
  • mbcH
 72248 - 73339 (-) mbcH
  • Precursor biosynthesis
Sequence-based prediction
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  • ACF35457.1
  • mbcI
 73336 - 74493 (-) mbcI
  • Precursor biosynthesis
Sequence-based prediction
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  • ACF35458.1
  • mbcJ
 74490 - 74765 (-) mbcJ
  • Precursor biosynthesis
Sequence-based prediction
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  • ACF35459.1
  • mbcK
 74762 - 75628 (-) mbcK
  • Precursor biosynthesis
Sequence-based prediction
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  • ACF35460.1
  • mbcG
 75881 - 76537 (+) mbcG
  • Precursor biosynthesis
Sequence-based prediction
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  • ACF35461.1
  • mbcP
 76534 - 77802 (+) mbcP
  • Tailoring (Unknown)
Knock-out
Desaturase; Bioinformatic analysis plus studies of other post-PKS genes confirmed function inc. complementation
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  • ACF35462.1
  • mbcS
 77831 - 79054 (+) mbcS
  • Tailoring (Monooxygenation)
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Complementation studies used to verify structure
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  • ACF35463.1
  • mbcT
 79119 - 79934 (+) mbcT
  • Tailoring (Methylation)
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Complementation studies used to verify function and regiochemistry
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  • ACF35464.1
  • mbcU
 79931 - 80716 (+) mbcU
  • Tailoring (Methylation)
Knock-out
Complementation studies used to verify function and regiochemistry
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Polyketide-specific information
Subclass Ansamycin
Starter unit 3,5-AHBA-CoA
Cyclic? yes
Release type
  • Macrolactamization
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: SOJ5J6FNXPWDFJNGJGPS2QRA, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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