BGC0000097: ML-449 biosynthetic gene cluster from Streptomyces sp. MP39-85
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Location: 1 - 89,357 nt. (total: 89,357 nt).
This entry is originally from NCBI GenBank FJ872525.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
KnownClusterBlast
NRPS/PKS domains
General information about the BGC
MIBiG accession BGC0000097
Short description ML-449 biosynthetic gene cluster from Streptomyces sp. MP39-85
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • PKS (Type I)
Loci
FJ872525.1
via Knock-out studies
Compounds
  • ML-449
Species Streptomyces sp. MP39-85 [taxonomy]
References
Chemical products information
ML-449 Evidence:
Copy SMILES
C29H39N1O3
Chemical database entries
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • ACO94480.1
 530 - 1102 (-) putative lipoprotein
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    • ACO94481.1
    		 1290 - 1838 (-) hypothetical protein
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      • ACO94482.1
      • mlaH
      		 3783 - 6638 (+) LuxR-type transcriptional regulator
      • Regulation
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      • ACO94483.1
      • mlaA1
      		 7108 - 26259 (+) polyketide synthase type I
      • Precursor biosynthesis
      • Knock-out
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      • ACO94484.1
      • mlaA2
      		 26297 - 29428 (+) polyketide synthase type I
      • Precursor biosynthesis
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      • ACO94485.1
      • mlaI
      		 29667 - 30758 (-) glycine oxidase/FAD-dependent oxidoreductase
      • Precursor biosynthesis
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      • ACO94486.1
      • mlaC
      		 30755 - 32842 (-) polyketide synthase type I
      • Precursor biosynthesis
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      • ACO94487.1
      • mlaU
      		 32874 - 33437 (-) putative NRPS accessory protein
      • Precursor biosynthesis
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      • ACO94488.1
      • mlaB
      		 33434 - 44026 (-) polyketide synthase type I
      • Scaffold biosynthesis
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      • ACO94489.1
      • mlaJ
      		 44073 - 45671 (-) putative AMP-dependent acyl-CoA synthetase/ligase
      • Scaffold biosynthesis
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      • ACO94490.1
      • mlaK
      		 45898 - 46839 (+) acyltransferase
      • Scaffold biosynthesis
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      • ACO94491.1
      • mlaS
      		 46855 - 47091 (-) peptidyl carrier protein
      • Scaffold biosynthesis
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      • ACO94492.1
      • mlaL
      		 47209 - 48723 (+) NRPS adenylation domain protein
      • Activation / processing
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      • ACO94493.1
      • mlaM
      		 48706 - 49302 (-) TetR-type transcriptional regulator
      • Regulation
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      • ACO94494.1
      • mlaN
      		 49403 - 50974 (+) MFS-type efflux pump
      • Transport
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      • ACO94495.1
      • mlaO
      		 51086 - 52321 (-) P450 monooxygenase
      • Tailoring
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      • ACO94496.1
      • mlaD
      		 52371 - 62468 (-) polyketide synthase type I
      • Scaffold biosynthesis
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      • ACO94497.1
      • mlaP
      		 62476 - 63411 (-) putative L-amino acid amidase/proline iminopeptidase
      • Scaffold biosynthesis
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      • ACO94498.1
      • mlaG
      		 63505 - 69483 (-) polyketide synthase type I
      • Scaffold biosynthesis
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      • ACO94499.1
      • mlaF
      		 69491 - 79612 (-) polyketide synthase type I
      • Scaffold biosynthesis
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      • ACO94500.1
      • mlaE
      		 79662 - 84575 (-) polyketide synthase type I
      • Scaffold biosynthesis
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      • ACO94501.1
      • mlaT
      		 85138 - 85404 (-) SimX2-like protein
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        • ACO94502.1
        • mlaQ
        		 85808 - 86578 (+) thioesterase type II
        • Scaffold biosynthesis
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        • ACO94503.1
        		 86721 - 87437 (+) PlsC-type phospholipid/glycerol acyltransferase
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          Biosynthesis information

          Biosynthetic modules

          Name
          1
          Type
          pks-modular
          Genes
          mlaA1
          Substrates
          Integrated Monomers
          Domains
          acyltransferase, ketosynthase, acyltransferase, carrier (ACP)
          Name
          2
          Type
          pks-modular
          Genes
          mlaA1
          Substrates
          Integrated Monomers
          Domains
          acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
          Name
          3
          Type
          pks-modular
          Genes
          mlaA1
          Substrates
          Integrated Monomers
          Domains
          acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
          Name
          4
          Type
          pks-modular
          Genes
          mlaA1
          Substrates
          Integrated Monomers
          Domains
          acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
          Name
          5
          Type
          pks-modular
          Genes
          mlaA2 mlaC
          Substrates
          Integrated Monomers
          Domains
          acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
          Name
          6
          Type
          pks-modular
          Genes
          mlaB
          Substrates
          Integrated Monomers
          Domains
          acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
          Name
          7
          Type
          pks-modular
          Genes
          mlaB
          Substrates
          Integrated Monomers
          Domains
          acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
          Name
          8
          Type
          pks-modular
          Genes
          mlaD
          Substrates
          Integrated Monomers
          Domains
          acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
          Name
          9
          Type
          pks-modular
          Genes
          mlaD
          Substrates
          Integrated Monomers
          Domains
          acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
          Name
          10
          Type
          pks-modular
          Genes
          mlaE
          Substrates
          Integrated Monomers
          Domains
          acyltransferase, ketosynthase, acyltransferase, ketoreductase, carrier (ACP)
          Name
          11
          Type
          pks-modular
          Genes
          mlaF
          Substrates
          Integrated Monomers
          Domains
          acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
          Name
          12
          Type
          pks-modular
          Genes
          mlaF
          Substrates
          Integrated Monomers
          Domains
          acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
          Name
          13
          Type
          pks-modular
          Genes
          mlaG
          Substrates
          Integrated Monomers
          Domains
          acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, thioesterase, carrier (ACP)
          Annotation changelog

          Entry version: 4

          Date
          Changes
          Submitters
          Reviewers
          2022-10-07
          Update chemical activity to schema version 2.11
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

          Entry version: 3

          Date
          Changes
          Submitters
          Reviewers
          2022-09-15
          Removed gene names of 'No gene ID'
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
          2022-09-15
          Removed duplicate gene name
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
          2022-09-15
          Removed ketoreductase stereochemistry annotation from modules without ketoreductases
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
          2022-09-15
          Sorted modules by module number
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
          2022-09-15
          Corrected module numbering
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

          Entry version: 2

          Date
          Changes
          Submitters
          Reviewers
          2019-10-16
          Migrated from v1.4
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
          2019-10-16
          Updated compound(s) information (MIBiG Annotathon)
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
          2019-10-16
          Updated compound(s) information (NPAtlas curation)
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

          Entry version: 1

          Date
          Changes
          Submitters
          Reviewers
          2015-06-12
          Submitted
          • (ID: MU4LZLKCEF7Q67JF6ZEMKX3R)
          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
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