Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000097.1:
Cluster 1Polyketide189357

BGC0000097, ML-449 biosynthetic gene cluster from Streptomyces sp. MP39-85. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: ML-449
Molecular formula: C29H39NO3
Exact molecular mass: 449.2924 Da (None)
Molecular activity: Antibacterial, Antifungal
Molecular target: unknown

Class-specific details

Biosynthetic class(es):
Polyketide
Polyketide subclass:
Other (cyclic)
Polyketide synthase subclass:
Modular type I
Starter unit:
Methylmalonyl-CoA
Thioesterase type:
Type I
Release / cyclization type:
Macrolactamization

Modular polyketide synthases:
mlaE (ACO94500.1)
Module 11
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None

mlaF (ACO94499.1)
Module 2
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 3
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None

mlaG (ACO94498.1)
Module 4
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None

mlaD (ACO94496.1)
Module 10
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 9
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None

mlaB (ACO94488.1)
Module 7
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 8
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None

mlaC (ACO94486.1)
Module 6
KR stereochemistry: Unknown
Scaffold-modifying domain: None

mlaA2 (ACO94484.1)
Module 5
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Scaffold-modifying domain: None

mlaA1 (ACO94483.1)
Module 1
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Scaffold-modifying domain: None
Module is non-elongating
Evidence for non-elongation: Unknown
Module 2
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 3
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 4
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None

Gene cluster description

ML-449 (BGC0000097). Gene Cluster 1. Biosynthetic class = Polyketide. GenBank FJ872525. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies
MIxS-compliance:Unknown
Contact for this cluster:Sergey Zotchev (Norwegian University of Science and Technology)

Literature references

1. Jorgensen H et al. (2010) Insights into the evolution of macrolactam biosynthesis through cloning and comparative analysis of the biosynthetic gene cluster for a novel macrocyclic lactam, ML-449. Appl Environ Microbiol 76(1):283-93. doi: 10.1128/AEM.00744-09. Epub