LOCUS       BGC0000099              8104 bp    DNA     linear   PLN 03-JUN-2010
DEFINITION  Penicillium marneffei strain PM1 putative polyketide synthase PKS3
            gene, complete cds.
ACCESSION   BGC0000099
VERSION     BGC0000099
KEYWORDS    .
SOURCE      Talaromyces marneffei (anamorph: Penicillium marneffei)
  ORGANISM  Talaromyces marneffei
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Eurotiales; Trichocomaceae;
            Talaromyces; Talaromyces sect. Talaromyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 16:01:57
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8104
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /country="China: Hong Kong"
                     /db_xref="taxon:37727"
                     /host="Homo sapiens; HIV-negative patient suffering from
                     culture-documented penicilliosis"
                     /mol_type="genomic DNA"
                     /note="anamorph: Penicillium marneffei"
                     /organism="Talaromyces marneffei"
                     /strain="PM1"
     mRNA            join(<1..618,671..>8104)
                     /product="putative polyketide synthase PKS3"
     protocluster    1..8104
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[0:8104]"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..8104
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     cand_cluster    1..8104
                     /SMILES="C([*])C(-O)"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..8104
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000099"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..8104
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             join(1..618,671..8104)
                     /NRPS_PKS="Domain: SAT (96-224). E-value: 3.3e-15. Score:
                     48.1. Matches aSDomain: nrpspksdomains_ADH01663.1_SAT.1"
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (378-794). E-value:
                     1.2e-141. Score: 464.3. Matches aSDomain:
                     nrpspksdomains_ADH01663.1_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (899-1191). E-value: 7.4e-68.
                     Score: 220.9. Matches aSDomain:
                     nrpspksdomains_ADH01663.1_PKS_AT.1"
                     /NRPS_PKS="Domain: PP-binding (1659-1725). E-value:
                     1.8e-13. Score: 42.7. Matches aSDomain:
                     nrpspksdomains_ADH01663.1_PP-binding.1"
                     /NRPS_PKS="Domain: PP-binding (1778-1838). E-value:
                     2.9e-11. Score: 35.6. Matches aSDomain:
                     nrpspksdomains_ADH01663.1_PP-binding.2"
                     /NRPS_PKS="Domain: cMT (2045-2235). E-value: 2.2e-57.
                     Score: 185.8. Matches aSDomain:
                     nrpspksdomains_ADH01663.1_cMT.1"
                     /NRPS_PKS="Domain: TD (2313-2560). E-value: 1.8e-27. Score:
                     88.0. Matches aSDomain: nrpspksdomains_ADH01663.1_TD.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     itr_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 215.2; E-value:
                     2.7e-65)"
                     /gene_kind="biosynthetic"
                     /product="putative polyketide synthase PKS3"
                     /protein_id="ADH01663.1"
                     /sec_met_domain="PP-binding (E-value: 3.2e-15, bitscore:
                     42.7, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 1.4e-69, bitscore: 220.9,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="itr_KS (E-value: 8.9e-153, bitscore:
                     495.1, seeds: 96, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 1.3e-26, bitscore:
                     79.4, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=1
                     /translation="MVSRIVNPSQGNTVLLFGPQALSFDQEAFLKLRATILGSPAYQWV
                     LNVVDELPIVWTSLLKHNPRLEAIDGAKELHSLVDWIKFGDIRGDCFPLPNILLSPLVV
                     ITQLTQYANYIQEHPDVRKSDKTQTLGFCTGILSALAVSSSADETGFAKYGSVAVRLAM
                     VIGAVVDAQDAPTERGPSKSLATAWNSANGEDELKRILAEFSDAYISVSYDQRRATVTT
                     STKTIAALQKELRGAGIIANEVGLVGRFHSDSHRKDLDSVIDFTNTQLDFQFPELSELH
                     LPTSFTTQIEDLANSKLHHAALRQILVDKSEWHQLFESVQEKSLSDKASLLLSFGPERP
                     VPPSIMRVLSSQVIHIADVNGKTIQQPNTNPHLIGDNDIAVVGMSLKVAGADDVEEFWD
                     LLLRGESQHQEVPKERITFETIFRDVDPKRKWFGNFIRDHDAFDHKFFKKSPREIGSTD
                     PQQRHMLQCAYQAVEQSGYFRSKNVNKKVGCYVGVCAADYENNIACHAPNAFSATGNLK
                     SFIAGKISHYFGWTGPGLTIDTACSSSAVAVHQACRAILSGECSAALAGGTNIMTNPLW
                     FQNLAGASFLSQTGQCKPFDAKADGYCRGEGIAAVFLKKLSSAIEDGDQILATIAGSAV
                     YQNQNCTPIFVPNAPSLSDLFRNVVAQARLEPKQITVVEAHGTGTAVGDPAEYESIHNV
                     LGGINRTTALQLSSVKGLIGHTECTSGLISLVKTVLMIQEARIPAQASFSSINPHIHHS
                     PDHNMEIPTKVLTWNENFRAALINNYGASGSNASMVVVQAPFTDKLAPAMEGSKSPFWF
                     TGPDDRSLRAYASRFVRFLQSKSVSSKSLTLGNVSFNVSRQSNRIHDKGLIFNAASLQE
                     LEQKLSTFASGESELASQSKLATKPVILCFGGQVSTFVGLDQQLYDNVTILRQHLNECD
                     AIVVKLGHSSIFPGVFQKTSVEDPVHLQTMLFAVQYSSAKAWIESGVEPAAVVGHSFGE
                     LTALCVSGALSLEDTVKMIIGRAKIIRENWGSEKGAMMAVEGDLEQVQNLLSESVVTGE
                     VAATIACYNGPRSFTLAGSMKAIEGVVKTASTGTFASMRTKVLNVTNAFHSTLVEPLME
                     DLDKVGGELTFCQPTIRLERATETVLSGDLTSKFVAEHMRHPVYFNHAVQRLAQEFPSS
                     IWIEAGSNSTITNMASRALGSSGGHHFQAINITNTSSGLSNLSDATVTLWKNGLDISFW
                     AHSSKQTYSYAPLLLPPYQFEKSRHWIEMKQPPKASGEPTVIEVVKTTVEELPKTLLTF
                     VGHQDTQKRQPRFRINTMIPKYEELVTGHVIANTAAICPATVEVDLAIEGLRTLYPGVT
                     DTMQPQIHSVDNQAPICVDPSRSVWLEYEALDDAAHTWNFKIYSTGNGKAATTTHVTGK
                     LIFRGLDDPQAQADFSRYERFVSHERCVALLKTDDADDVIQGRNIYKTFGEIVDYGEQY
                     RGLQKLVGKGTESAGRVVMKPSTETWLDAHLSDAFSQVGGIWVNCMTDREPADMYIANG
                     FEQWIRSPKVKSTVERPASWDVFALHQKHGDKAYVTDIFIFNGLTGDLTEVILGINYAK
                     VAKMSMSKLLSRLTPNGSGVGSAAPSAAAKVQAGTAVTAFTPATSSNVGSLPAVTKPRK
                     TPKAPKAPKKKVSSGQPEITGQVRAMLAELSGLEPEEIKEDVELANIGIDSLMGMELAR
                     EIENIFKCSLPEDQLVEVTTFQALLQCIRANLPASAGAAEEIEDDDDEEEEDEDDDESL
                     EDARVFTPSDAATSVSSAGKADVIEFLVEFLGIEESSVTRSTLLRDLGVDSLLSTELRA
                     DISSKFGVDVPEEVMLEDLSVEEFDVQINGSSSSKAEPPKPAPSVSVPGVSSSSSNAPR
                     ATGGNLDLPASVILEAFHETKVLTDKFIEDYRCAGYIDNVLPKQDELCIALAVEAFEQL
                     GVSLKGAKPGQKLEPIKYLPQHNRLAEYLYMMLEKGARLIDIDANGVITRTAVATPTKS
                     SNDICQQLVRDYPDHNFANRLTQFCGLRLADVLTGKMDGIKLIFGSAEGRELVSGLYGD
                     SLLNKLAYEQMKDFLRRLISKLPVNEGPLKILEMGAGTGGTTKWIVPLLAELNFPIEYT
                     YTDLAPSFVAAARQKFKAYPFMKFRVHDIEKQPADDLLGTQHLILASNAVHATHNLTVS
                     TQNIRKALRPDGFLMMLEMTETVYWIDVIFGLLEGWWLFEDGRRHAIAHQSRWEKDLKS
                     VGFGHVDYTDGHRPEVNIQRIFIALASGQRYDRIPIPPKAVTNEAPKTDILARKAAVSK
                     YLQKAVSGFTAVSISSPAHPVLSNQGVLVTGATGSVGSHLVAHLANLPSVKTVFCINRV
                     SRSDASKRQQEALLSKGISLTPAAMSKLVVIETDTHKPLLGLQRGEYDRLVRDVSHIVH
                     NAWPMSGKRALMGFEQQFVVMRNLINLANEASANGPRRITFQLISSISTVGYYPIWSGE
                     RNVPEIRVEMNSVLDNGYSDAKLVCERMLDETLHKHPDRFRPMAVRLGQVAGSSSGGYW
                     NPMEHFSFLIKSSQTLRAFPALEGELSWTPVDAVAATLGDLISGDHRPYPVYHIDNPVR
                     QQWRDTIPVLAEALGIPSSNVIPFKDWVGMVRRFPGSEVDNPAVRLIDFLDDNFLRMSC
                     GGLLLDTTKSREHSPTLAAVGPVSADVTRQYVKAWRDMGFLY"
     aSDomain        join(289..618,671..724)
                     /aSDomain="SAT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ADH01663.1_SAT.1"
                     /evalue="3.30E-15"
                     /label="ADH01663.1_SAT.1"
                     /locus_tag="ADH01663.1"
                     /protein_end="224"
                     /protein_start="96"
                     /score="48.1"
                     /tool="antismash"
                     /translation="ILLSPLVVITQLTQYANYIQEHPDVRKSDKTQTLGFCTGILSALA
                     VSSSADETGFAKYGSVAVRLAMVIGAVVDAQDAPTERGPSKSLATAWNSANGEDELKRI
                     LAEFSDAYISVSYDQRRATVTTST"
     aSDomain        1187..2434
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ADH01663.1_PKS_KS.1"
                     /domain_subtype="Iterative-KS"
                     /evalue="1.20E-141"
                     /label="ADH01663.1_PKS_KS.1"
                     /locus_tag="ADH01663.1"
                     /protein_end="794"
                     /protein_start="378"
                     /score="464.3"
                     /tool="antismash"
                     /translation="IAVVGMSLKVAGADDVEEFWDLLLRGESQHQEVPKERITFETIFR
                     DVDPKRKWFGNFIRDHDAFDHKFFKKSPREIGSTDPQQRHMLQCAYQAVEQSGYFRSKN
                     VNKKVGCYVGVCAADYENNIACHAPNAFSATGNLKSFIAGKISHYFGWTGPGLTIDTAC
                     SSSAVAVHQACRAILSGECSAALAGGTNIMTNPLWFQNLAGASFLSQTGQCKPFDAKAD
                     GYCRGEGIAAVFLKKLSSAIEDGDQILATIAGSAVYQNQNCTPIFVPNAPSLSDLFRNV
                     VAQARLEPKQITVVEAHGTGTAVGDPAEYESIHNVLGGINRTTALQLSSVKGLIGHTEC
                     TSGLISLVKTVLMIQEARIPAQASFSSINPHIHHSPDHNMEIPTKVLTWNENFRAALIN
                     NYGASGSNASMVVVQAP"
     aSModule        1187..5227
                     /complete
                     /domains="nrpspksdomains_ADH01663.1_PKS_KS.1"
                     /domains="nrpspksdomains_ADH01663.1_PKS_AT.1"
                     /domains="nrpspksdomains_ADH01663.1_PP-binding.1"
                     /iterative
                     /locus_tags="ADH01663.1"
                     /monomer_pairings="pk -> pk"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       1646..1693
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ADH01663.1_0001"
                     /evalue="4.10E-05"
                     /label="PKSI-KS_m3"
                     /locus_tag="ADH01663.1"
                     /protein_end="547"
                     /protein_start="531"
                     /score="15.8"
                     /tool="antismash"
                     /translation="GPGLTIDTACSSSAVA"
     CDS_motif       1835..1873
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ADH01663.1_0002"
                     /evalue="5.70E-05"
                     /label="PKSI-KS_m4"
                     /locus_tag="ADH01663.1"
                     /protein_end="607"
                     /protein_start="594"
                     /score="15.9"
                     /tool="antismash"
                     /translation="FDAKADGYCRGEG"
     CDS_motif       2069..2113
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ADH01663.1_0003"
                     /evalue="1.00E-03"
                     /label="PKSI-KS_m5"
                     /locus_tag="ADH01663.1"
                     /protein_end="687"
                     /protein_start="672"
                     /score="11.5"
                     /tool="antismash"
                     /translation="VEAHGTGTAVGDPAE"
     CDS_motif       2195..2221
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ADH01663.1_0004"
                     /evalue="5.40E+01"
                     /label="PKSI-KS_m3"
                     /locus_tag="ADH01663.1"
                     /protein_end="723"
                     /protein_start="714"
                     /score="-2.2"
                     /tool="antismash"
                     /translation="HTECTSGLI"
     aSDomain        2750..3625
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ADH01663.1_PKS_AT.1"
                     /evalue="7.40E-68"
                     /label="ADH01663.1_PKS_AT.1"
                     /locus_tag="ADH01663.1"
                     /protein_end="1191"
                     /protein_start="899"
                     /score="220.9"
                     /specificity="consensus: pk"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mxmal"
                     /tool="antismash"
                     /translation="CFGGQVSTFVGLDQQLYDNVTILRQHLNECDAIVVKLGHSSIFPG
                     VFQKTSVEDPVHLQTMLFAVQYSSAKAWIESGVEPAAVVGHSFGELTALCVSGALSLED
                     TVKMIIGRAKIIRENWGSEKGAMMAVEGDLEQVQNLLSESVVTGEVAATIACYNGPRSF
                     TLAGSMKAIEGVVKTASTGTFASMRTKVLNVTNAFHSTLVEPLMEDLDKVGGELTFCQP
                     TIRLERATETVLSGDLTSKFVAEHMRHPVYFNHAVQRLAQEFPSSIWIEAGSNSTITNM
                     ASRALGSSGGH"
     CDS_motif       2978..3097
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ADH01663.1_0005"
                     /evalue="4.00E-16"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="ADH01663.1"
                     /protein_end="1015"
                     /protein_start="975"
                     /score="51.1"
                     /tool="antismash"
                     /translation="GVEPAAVVGHSFGELTALCVSGALSLEDTVKMIIGRAKII"
     aSDomain        5030..5227
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ADH01663.1_PP-binding.1"
                     /evalue="1.80E-13"
                     /label="ADH01663.1_PP-binding.1"
                     /locus_tag="ADH01663.1"
                     /protein_end="1725"
                     /protein_start="1659"
                     /score="42.7"
                     /tool="antismash"
                     /translation="QVRAMLAELSGLEPEEIKEDVELANIGIDSLMGMELAREIENIFK
                     CSLPEDQLVEVTTFQALLQCI"
     aSDomain        5387..5566
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ADH01663.1_PP-binding.2"
                     /evalue="2.90E-11"
                     /label="ADH01663.1_PP-binding.2"
                     /locus_tag="ADH01663.1"
                     /protein_end="1838"
                     /protein_start="1778"
                     /score="35.6"
                     /tool="antismash"
                     /translation="VIEFLVEFLGIEESSVTRSTLLRDLGVDSLLSTELRADISSKFGV
                     DVPEEVMLEDLSVEE"
     CDS_motif       5624..5665
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ADH01663.1_0006"
                     /evalue="9.90E+01"
                     /label="PKSI-KS_m3"
                     /locus_tag="ADH01663.1"
                     /protein_end="1871"
                     /protein_start="1857"
                     /score="-4.4"
                     /tool="antismash"
                     /translation="PSVSVPGVSSSSSN"
     aSDomain        6188..6757
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ADH01663.1_cMT.1"
                     /domain_subtype="cMT"
                     /evalue="2.20E-57"
                     /label="ADH01663.1_cMT.1"
                     /locus_tag="ADH01663.1"
                     /protein_end="2235"
                     /protein_start="2045"
                     /score="185.8"
                     /tool="antismash"
                     /translation="SGLYGDSLLNKLAYEQMKDFLRRLISKLPVNEGPLKILEMGAGTG
                     GTTKWIVPLLAELNFPIEYTYTDLAPSFVAAARQKFKAYPFMKFRVHDIEKQPADDLLG
                     TQHLILASNAVHATHNLTVSTQNIRKALRPDGFLMMLEMTETVYWIDVIFGLLEGWWLF
                     EDGRRHAIAHQSRWEKDLKSVGFGHVD"
     aSModule        6188..7732
                     /domains="nrpspksdomains_ADH01663.1_cMT.1"
                     /domains="nrpspksdomains_ADH01663.1_TD.1"
                     /final_module
                     /incomplete
                     /locus_tags="ADH01663.1"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        6992..7732
                     /aSDomain="TD"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ADH01663.1_TD.1"
                     /evalue="1.80E-27"
                     /label="ADH01663.1_TD.1"
                     /locus_tag="ADH01663.1"
                     /protein_end="2560"
                     /protein_start="2313"
                     /score="88.0"
                     /tool="antismash"
                     /translation="VLVTGATGSVGSHLVAHLANLPSVKTVFCINRVSRSDASKRQQEA
                     LLSKGISLTPAAMSKLVVIETDTHKPLLGLQRGEYDRLVRDVSHIVHNAWPMSGKRALM
                     GFEQQFVVMRNLINLANEASANGPRRITFQLISSISTVGYYPIWSGERNVPEIRVEMNS
                     VLDNGYSDAKLVCERMLDETLHKHPDRFRPMAVRLGQVAGSSSGGYWNPMEHFSFLIKS
                     SQTLRAFPALEGELSWTPVDAVAAT"
ORIGIN
        1 atggtaagcc ggatagtcaa tccctctcag gggaacactg tccttctttt cggtcctcag
       61 gccctgtcat ttgatcagga agccttcctc aaattacgcg caaccattct cggctcgcct
      121 gcttaccaat gggttctcaa tgttgtcgat gagttgccta tcgtatggac aagccttcta
      181 aaacataacc ctagattgga ggcgatcgat ggagcaaagg aattacacag cctggttgac
      241 tggatcaaat tcggtgatat acgcggcgat tgtttccctc ttcccaacat tcttcttagc
      301 ccgcttgtcg tcatcaccca attgacgcaa tatgccaatt atatccaaga acacccagac
      361 gtccggaagt cagacaaaac acagactcta ggattttgca ccggtatact tagtgccctt
      421 gccgtatcca gcagcgctga tgagaccgga tttgcaaagt acggctcggt cgcagtacgc
      481 cttgcaatgg ttatcggagc tgttgtagac gcgcaagacg caccgaccga acggggtcct
      541 tcgaaatcat tggcaactgc ctggaactcc gccaacggtg aggacgaact aaagcgaatc
      601 ctggctgaat tctcagatgt aggcatattc atcccttcta tcttgctatg gttagttctg
      661 actctcatag gcctacattt cggttagcta tgaccagcgt cgtgcaactg tcacaacttc
      721 gaccaagacc attgcggctt tacaaaaaga actccgtggc gctggtatca ttgccaatga
      781 agttggtctc gttggccgtt tccacagcga ctcgcaccgg aaagatctgg attcagtcat
      841 tgactttacc aacacacaac tcgatttcca attcccagag ctgtcggagc tacatcttcc
      901 caccagcttc accacccaga ttgaggatct agctaacagc aagctacatc atgccgcatt
      961 gcgccaaata ttggttgata aatcggaatg gcatcaatta tttgagtccg tacaagaaaa
     1021 aagcttaagc gacaaggctt ccctccttct ttcatttggc ccggagcgac ccgtacctcc
     1081 ctctatcatg cgagtgctta gctctcaggt tattcatatt gctgatgtga acggaaaaac
     1141 tatccaacag cccaacacca acccgcacct gattggagat aacgatatcg cagttgttgg
     1201 catgtccctc aaagtcgctg gcgctgatga cgttgaggag ttctgggacc ttcttctcag
     1261 aggggaatcg caacaccaag aagttccaaa agagcgaatc acttttgaga ccatcttccg
     1321 agatgtcgat cccaagagga agtggtttgg aaacttcatt cgagaccatg atgcattcga
     1381 tcacaaattc ttcaagaaga gtccacgtga gatcggatcc acagaccctc aacaaaggca
     1441 tatgttgcag tgtgcttatc aggcggtcga gcaatctggt tattttcgat caaagaacgt
     1501 caataaaaaa gttgggtgct acgttggtgt ttgtgcagca gattatgaga acaacattgc
     1561 atgtcatgcc cctaacgcat tctcagcgac tggaaacttg aagagtttta tcgctggtaa
     1621 gatcagtcat tatttcggtt ggaccggccc cggcttgaca atcgacactg cttgctcatc
     1681 gtccgctgtc gctgttcacc aggcttgtcg agcaatcctc agcggtgaat gctcagcagc
     1741 gttggctgga ggaaccaata tcatgacaaa tccactttgg ttccaaaact tagcaggagc
     1801 ctcctttctc agtcaaactg ggcagtgtaa accatttgac gctaaggcag atggatactg
     1861 tcggggtgag ggtatcgcgg ccgtgttcct caagaagctg tcttctgcta tcgaagacgg
     1921 tgaccaaatt ttagcaacta ttgcaggctc tgctgtttac caaaaccaga actgtactcc
     1981 tatcttcgtt ccgaacgcac cttctctttc ggatctcttc cgtaatgtcg ttgctcaagc
     2041 tcgcctggag cccaaacaga ttactgttgt tgaagcacac ggtactggta cagcggttgg
     2101 tgatccggct gaatacgaga gtattcacaa tgtgctcggt ggaataaatc gaacaacagc
     2161 ccttcagctg tcgtctgtta agggcttgat tggccatacc gaatgtacat caggtttaat
     2221 ctccctcgtt aagaccgtcc tcatgattca agaggccaga attcccgcac aggccagttt
     2281 ctctagtatt aaccctcaca tacaccatag cccggaccac aacatggaaa taccaacaaa
     2341 agtcttgacc tggaatgaga atttccgggc tgccttgatc aacaactacg gagcctccgg
     2401 ttccaacgct tccatggtgg ttgtgcaggc tccatttacg gacaagttgg cacccgcaat
     2461 ggaaggctcc aagtctccat tctggttcac cggtccggat gatcggagtt tgagggctta
     2521 tgcatcgcgg ttcgtcaggt tcttgcaatc caaatcagtt tcttcgaaaa gcttgacatt
     2581 aggcaatgtt tccttcaacg tttcccgcca atccaacaga attcatgaca agggtctgat
     2641 cttcaatgct gcttctctgc aggagttaga acaaaaatta tccacctttg ccagtggcga
     2701 atctgagctt gcttctcagt caaaacttgc aaccaaacct gttatactct gctttggagg
     2761 tcaagtgtct acatttgttg gtctggacca acaactttac gataatgtga ccattctccg
     2821 ccaacacttg aatgagtgtg atgctatcgt ggttaagcta ggacatagca gtattttccc
     2881 tggagtcttc caaaaaacgt ctgtcgaaga tccggtgcac ttgcagacaa tgctctttgc
     2941 cgtccagtac tcgtccgcta aagcctggat tgagtctggt gttgaaccgg cagctgtcgt
     3001 tggtcacagc ttcggagaat tgactgcgct ttgcgtctcg ggagcgctta gcctcgaaga
     3061 cactgtcaag atgataattg gccgcgctaa gatcattcgt gagaattggg gttctgaaaa
     3121 gggggccatg atggcagttg aaggtgatct cgaacaggtt cagaacctgc tttcggagtc
     3181 ggttgttaca ggagaagtag cagcaaccat tgcttgttac aatggacccc gtagcttcac
     3241 acttgcgggt tctatgaaag ctattgaagg agtcgtcaag actgcatcca ccggtacttt
     3301 cgcctccatg cgtactaagg tgctgaatgt caccaatgcc ttccactcca ctttggtaga
     3361 gccattgatg gaagacttgg ataaagtagg aggtgagctg accttctgtc agcccactat
     3421 tcgcctcgag agagccactg agactgtctt gagtggagat ttaacctcca agtttgtcgc
     3481 agagcacatg cggcaccctg tctatttcaa tcatgccgtg caaagactcg ctcaagaatt
     3541 tccttccagt atttggatcg aggcgggctc caactcaacg attaccaaca tggctagtcg
     3601 tgctttggga tcttctggcg ggcaccattt tcaggctatc aacatcacga atacctccag
     3661 tggactgtca aatctctctg atgcaacagt gactttgtgg aagaacgggc tcgacatttc
     3721 tttctgggct cacagctcca agcaaacata ctcatatgcc ccgttattac ttccaccata
     3781 tcaattcgaa aaatctcggc attggattga aatgaaacag ccccctaagg ccagtgggga
     3841 gcccactgtt attgaagtag taaagacaac tgttgaggag cttcccaaga ctcttttgac
     3901 ctttgtcggt caccaagata cccagaagcg ccaacctcgt ttccgtatca atactatgat
     3961 acctaagtat gaagagttag tcaccggtca tgttattgcc aatactgctg caatttgtcc
     4021 tgcgactgtg gaagtcgatc tggctatcga gggtcttcgc acactttatc cgggagtgac
     4081 cgacactatg cagcctcaaa tacattcggt tgataaccaa gccccaatct gcgtggatcc
     4141 gtcgcgatct gtttggctcg agtatgaggc gcttgacgac gctgctcata cctggaactt
     4201 caagatctat agcactggca atggaaaggc tgcgacgacc acccatgtta ctggaaagct
     4261 tattttccgg ggcttagatg acccccaagc tcaggctgac ttttccagat atgagcgttt
     4321 cgtgagccac gaacgttgtg tggctttact caaaacggac gatgcagacg acgtaattca
     4381 gggtcgcaac atctacaaga cttttggtga aatcgtcgat tacggtgaac aatatcgcgg
     4441 cttgcagaaa ctggtcggta aaggtaccga gtctgctggt agagtcgtca tgaagccatc
     4501 aactgaaacg tggctggatg cccatttgtc cgacgctttc agtcaagttg gcggaatatg
     4561 ggtcaattgt atgacggacc gcgagccggc cgacatgtac attgcaaatg gctttgaaca
     4621 atggattcga tcacccaaag taaaatctac cgttgagcgg cctgcgtcat gggatgtgtt
     4681 tgctcttcac caaaagcacg gcgacaaggc ttatgtcaca gatattttta tcttcaatgg
     4741 cctcaccggt gacctcaccg aggttattct tggcatcaat tatgcaaagg tcgcaaagat
     4801 gtctatgagc aagctgctta gccgtcttac acctaacggt agtggagttg gttctgccgc
     4861 gccttcggca gcagccaagg tgcaagctgg gaccgccgtg actgcattta ctccagctac
     4921 atcatcgaac gttgggtctc ttcctgctgt gacaaagcct cggaaaaccc cgaaagcacc
     4981 caaagcaccc aagaagaagg tttcgtctgg ccagccggaa atcactggtc aggttcgagc
     5041 aatgctggca gagctgtcag gccttgagcc cgaagaaatc aaagaggacg ttgagcttgc
     5101 aaacattgga attgattctc tgatgggcat ggagcttgca cgagaaattg agaatatttt
     5161 caaatgctca ttgcctgaag atcaacttgt ggaggtaacc acattccaag cactcttgca
     5221 gtgtattcgg gccaatctcc cagcatctgc aggtgcggcc gaagagattg aagacgacga
     5281 tgacgaggaa gaggaagatg aagacgacga cgagtctctc gaagatgcaa gggtgttcac
     5341 accatccgat gccgctactt ccgtttcgtc agctggcaaa gcagatgtca ttgaattcct
     5401 cgttgagttc ttgggcattg aggagagcag cgtcacccga agcacacttc tccgcgacct
     5461 tggtgttgac tccttgcttt cgactgaact acgcgctgat atctcgtcca agttcggcgt
     5521 cgacgtgcca gaggaggtga tgctggaaga tctgtcagtc gaagagtttg acgtccagat
     5581 caacggatct tcttcctcca aggctgagcc acccaaacca gctccttccg tttcggtacc
     5641 cggggtttct tcttcctctt ccaacgcgcc acgtgccaca ggaggcaacc tcgacctccc
     5701 agcttcggtt atccttgagg ctttccacga aacgaaggtg ctgactgaca aattcatcga
     5761 agactaccgc tgtgccggtt acatcgacaa tgttctgccc aagcaagatg agctttgcat
     5821 tgctctggcc gtcgaggcct ttgaacaact tggagtatcc ctcaaaggag ccaagccagg
     5881 tcaaaagttg gaacctatca agtatttgcc tcagcataat cgacttgcgg agtacctcta
     5941 catgatgcta gagaaaggag ctcgcttgat agacattgac gcgaacggtg tgattacaag
     6001 gactgccgtg gctacaccaa ccaaatcgag caacgacatc tgccagcagt tggttagaga
     6061 ttaccccgat cataactttg caaacagact cacccaattc tgtggtcttc gacttgctga
     6121 cgtcctgact ggcaagatgg acggcatcaa gctcattttc ggatccgccg aaggacgtga
     6181 actagtctct ggtctttacg gtgattctct attgaacaag ttggcctacg aacagatgaa
     6241 agacttcctt cgtcgtctga tatcgaaact accagtgaac gaagggccat taaagatctt
     6301 ggagatgggt gctggcactg gaggcaccac caaatggatt gtaccattgc tggctgagtt
     6361 gaacttccct atcgagtata catacaccga tttggcacca tcttttgtgg ccgccgctcg
     6421 tcaaaagttc aaagcctatc cgttcatgaa gttccgtgtc cacgacattg agaaacagcc
     6481 agcagatgat ctgcttggaa ctcagcatct aattctcgca agcaatgctg tccatgccac
     6541 ccacaatctt actgtctcta cgcaaaacat tcgcaaggca ctacgccctg acgggttcct
     6601 aatgatgctt gagatgactg aaactgtata ctggattgat gtgatctttg gtctacttga
     6661 aggctggtgg ctgtttgagg acggccgccg tcacgcaatt gcccaccagt cacggtggga
     6721 gaaggacctc aagtcggtag gctttggaca tgtcgactat actgatggac accgtccaga
     6781 ggtcaatatc cagcgcatct ttatcgcgct ggcttccggc cagaggtacg accgaatccc
     6841 tatccctccc aaggctgtta cgaatgaagc tccgaaaact gacattctgg cacgtaaagc
     6901 ggccgtcagt aaatatcttc agaaagcagt cagtggcttt accgcagtct ctatctcctc
     6961 acctgcccac cctgtactct caaatcaagg cgtgcttgtc acaggagcta ctgggagcgt
     7021 cggatctcat ctagttgctc acttggccaa tctaccctct gtcaagacag tgttctgtat
     7081 caatcgagtc agtcgctcag atgcatcaaa acgccagcaa gaagcactcc tgtccaaagg
     7141 catttcattg actcccgcag caatgtcaaa gctagtggtg attgaaacgg atacgcacaa
     7201 acctctgctc ggtcttcagc gtggtgaata cgatcgtcta gtccgcgatg tttctcacat
     7261 cgtgcacaat gcttggccaa tgagcggcaa acgagcgttg atgggcttcg agcagcaatt
     7321 cgttgtcatg agaaatctca tcaatcttgc aaacgaagct tcagcaaacg gtccacggag
     7381 aatcaccttc cagcttatat cttcaatctc tacagtgggc tattacccga tatggagtgg
     7441 agagcgtaac gtaccggaaa ttcgtgtcga gatgaactct gtacttgaca acggatacag
     7501 cgatgccaaa ctggtttgtg agcgaatgtt ggatgagaca cttcataaac atcccgaccg
     7561 attccggccg atggccgttc gattaggtca ggtcgccgga tctagctcag gcggttactg
     7621 gaatccaatg gagcactttt ctttcctaat caaatcgtca caaacactga gggcttttcc
     7681 agctcttgaa ggtgaacttt cctggacacc tgttgatgcg gttgcagcta cactaggtga
     7741 tttgatctct ggtgaccatc gtccttaccc agtttaccac atcgacaacc ctgttcgtca
     7801 gcaatggcgg gatacaattc cagtactcgc tgaggctctg ggcattcctt caagcaacgt
     7861 tattcccttc aaagactggg tgggcatggt gcgacgcttc ccggggtctg aggtggacaa
     7921 ccctgcggta cggcttattg atttcttgga tgataatttt ttgagaatgt cgtgtggtgg
     7981 attgctcctt gatactacaa agagccgcga gcattctccg acattagcag cagttggtcc
     8041 agtgagcgca gacgttacac ggcaatatgt aaaagcatgg agggatatgg gattcttgta
     8101 ctga
//
