BGC0000101 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000101 (Aspergillus nidulans FGSC A4)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	45,562	High	emodin/monodictyphenone/shamixanthone	PKS:Iterative type I

BGC0000101 - Region 1 - T1PKS

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Location: 1 - 45,562 nt. (total: 45,562 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 45,563
Label		BGC0000101

Tailoring functions

Oxidoreductases: Monooxygenation dehydrogenase

ANIA_00146: Biaryl bond formation, Reduction

MITE: MITE0000160Biaryl bond formation, Reduction (identity: 74.30%)
smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

ANIA_00147: Monooxygenation

MITE: MITE0000156Monooxygenation (identity: 60.30%)

Transferases: methyltransferase sulfotransferase

ANIA_10021: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

ANIA_10038: glutathione S-transferase

smcogs: SMCOG1193: glutathione S-transferase

Hydrolases: hydrolase

ANIA_00149: Decarboxylation

MITE: MITE0000163Decarboxylation (identity: 67.30%)
smcogs: SMCOG1170: metallo-beta-lactamase family protein

Lyases: hydrolase

ANIA_00149: Decarboxylation

MITE: MITE0000163Decarboxylation (identity: 67.30%)
smcogs: SMCOG1170: metallo-beta-lactamase family protein

Ligases: dehydrogenase ligase synthase

ANIA_00146: Biaryl bond formation, Reduction

MITE: MITE0000160Biaryl bond formation, Reduction (identity: 74.30%)
smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

ANIA_00150: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ANIA_10035: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000101.5	0.99	PKS	emodin, monodictyphenone, shamixanthone	Aspergillus nidulans FGSC A4
BGC0000013.3	0.69	PKS	alternariol	Aspergillus nidulans FGSC A4
BGC0001284.4	0.68	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.65	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001258.3	0.65	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0002154.2	0.65	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.65	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002175.3	0.65	PKS	YWA1	Aspergillus oryzae RIB40

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000101.5	0.99	PKS	emodin, monodictyphenone, shamixanthone	Aspergillus nidulans FGSC A4
BGC0000013.3	0.69	PKS	alternariol	Aspergillus nidulans FGSC A4
BGC0001284.4	0.68	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.65	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001258.3	0.65	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0002154.2	0.65	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.65	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002175.3	0.65	PKS	YWA1	Aspergillus oryzae RIB40

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 45562:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ANIA_10035: X

AMP-binding (83..493): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GIAVQGNGAK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

ANIA_00150: mal

PKS_AT (952..1250): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 88.2
inactive: 63.2
Methylmalonyl-CoA: 58.5
Methoxymalonyl-CoA: 51.5
Propionyl-CoA: 34.3
2-Methylbutyryl-CoA: 30.6
Ethylmalonyl-CoA: 28.4
CHC-CoA: 25.0
Isobutyryl-CoA: 24.6
Benzoyl-CoA: 24.1
fatty_acid: 22.0
Acetyl-CoA: 15.7
3-Methylbutyryl-CoA: 15.6
trans-1,2-CPDA: 14.4

Link to PARAS substrate predictor