BGC0000109 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000109 (Streptomyces cyaneogriseus subsp. noncyanogenus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	80,358	High	LL-F28249α	PKS

BGC0000109 - Region 1 - T1PKS

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Location: 1 - 80,358 nt. (total: 80,358 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 80,359
Label		BGC0000109

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

BAF85845.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

nemE: Heterocyclization, Hydroxylation

MITE: MITE0000010Heterocyclization, Hydroxylation (identity: 60.00%)
smcogs: SMCOG1034: cytochrome P450

nemF: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: methyltransferase

nemD: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: esterase

nemG: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: synthase

nemA1-1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

nemA1-2: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

nemA2: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

nemA3: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

nemA4: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000109.5	1.00	PKS	LL-F28249α	Streptomyces cyaneogriseus subsp. noncyanogenus
BGC0000041.3	0.78	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001254.3	0.64	PKS	ACT-Toxin II	Alternaria alternata
BGC0002192.3	0.64	PKS	FR901512	fungal sp. No.14919
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000109.5	1.00	PKS	LL-F28249α	Streptomyces cyaneogriseus subsp. noncyanogenus
BGC0000041.3	0.78	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001254.3	0.64	PKS	ACT-Toxin II	Alternaria alternata
BGC0002192.3	0.64	PKS	FR901512	fungal sp. No.14919
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 80358:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (emal - Me-ccmal) + (Me-ohmal - redmal) + (mal - ohmal - ohmal - Me-ccmal) + (Me-redmal - ccmal - Me-ccmal) + (ohmal - Me-ohmal - ccmal)
Non-colinear ordering:: nemA1-1 + nemA1-2 + nemA2 + nemA3 + nemA4

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

nemA1-1: emal - mmal

PKS_AT (22..329): emal

ATSignature: 2-Methylbutyryl-CoA

Top 3 matches:: 2-Methylbutyryl-CoA: 83.3%; Methoxymalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 66.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 165.7
Methylmalonyl-CoA: 165.2
2-Methylbutyryl-CoA: 163.0
Methoxymalonyl-CoA: 143.5
trans-1,2-CPDA: 125.9
Propionyl-CoA: 115.2
CHC-CoA: 106.6
Malonyl-CoA: 88.1
Benzoyl-CoA: 63.7
Isobutyryl-CoA: 56.8
Acetyl-CoA: 32.2
inactive: 26.8
3-Methylbutyryl-CoA: 23.7
fatty_acid: 16.4

PKS_AT (1009..1301): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.1
Ethylmalonyl-CoA: 211.6
Methoxymalonyl-CoA: 181.3
2-Methylbutyryl-CoA: 149.3
trans-1,2-CPDA: 131.1
Propionyl-CoA: 124.8
Malonyl-CoA: 103.9
CHC-CoA: 93.4
Isobutyryl-CoA: 80.3
Benzoyl-CoA: 68.3
Acetyl-CoA: 53.2
3-Methylbutyryl-CoA: 48.5
fatty_acid: 32.1
inactive: 30.4

nemA1-2: mmal - mal

PKS_AT (574..866): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 211.0
Ethylmalonyl-CoA: 206.6
Methoxymalonyl-CoA: 173.0
2-Methylbutyryl-CoA: 146.4
trans-1,2-CPDA: 121.9
Propionyl-CoA: 113.0
Malonyl-CoA: 96.4
Isobutyryl-CoA: 91.7
CHC-CoA: 91.1
Benzoyl-CoA: 75.2
Acetyl-CoA: 59.3
3-Methylbutyryl-CoA: 39.0
inactive: 29.5
fatty_acid: 27.8

PKS_AT (2137..2418): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 114.1
Methoxymalonyl-CoA: 51.5
inactive: 42.6
Methylmalonyl-CoA: 32.2
Ethylmalonyl-CoA: 17.4
Propionyl-CoA: 14.4
fatty_acid: 6.7
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0
Acetyl-CoA: -2.0

nemA2: mal - mal - mal - mmal

PKS_AT (583..864): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 120.3
Methoxymalonyl-CoA: 50.7
inactive: 43.4
Methylmalonyl-CoA: 31.8
Ethylmalonyl-CoA: 17.0
Propionyl-CoA: 14.9
Acetyl-CoA: 12.8
fatty_acid: 7.5
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (1614..1895): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 123.2
Methoxymalonyl-CoA: 50.9
inactive: 44.3
Methylmalonyl-CoA: 32.1
Ethylmalonyl-CoA: 17.4
Propionyl-CoA: 13.7
Acetyl-CoA: 12.8
fatty_acid: 6.2
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (3169..3450): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 120.7
Methoxymalonyl-CoA: 50.3
inactive: 43.9
Methylmalonyl-CoA: 31.7
Ethylmalonyl-CoA: 17.0
Propionyl-CoA: 14.3
Acetyl-CoA: 12.8
fatty_acid: 7.0
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (4727..5019): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 224.1
Ethylmalonyl-CoA: 222.4
Methoxymalonyl-CoA: 181.8
2-Methylbutyryl-CoA: 147.5
trans-1,2-CPDA: 134.2
Propionyl-CoA: 115.7
Malonyl-CoA: 101.8
CHC-CoA: 95.7
Isobutyryl-CoA: 92.8
Benzoyl-CoA: 74.8
Acetyl-CoA: 56.6
3-Methylbutyryl-CoA: 39.4
fatty_acid: 37.5
inactive: 33.0

nemA3: mmal - mal - mmal

PKS_AT (574..866): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 224.2
Ethylmalonyl-CoA: 223.3
Methoxymalonyl-CoA: 181.8
2-Methylbutyryl-CoA: 148.3
trans-1,2-CPDA: 134.5
Propionyl-CoA: 116.3
Malonyl-CoA: 100.3
CHC-CoA: 97.1
Isobutyryl-CoA: 91.0
Benzoyl-CoA: 74.9
Acetyl-CoA: 53.5
3-Methylbutyryl-CoA: 40.5
fatty_acid: 37.5
inactive: 32.2

PKS_AT (2662..2943): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 120.3
Methoxymalonyl-CoA: 50.7
inactive: 43.4
Methylmalonyl-CoA: 31.8
Ethylmalonyl-CoA: 17.0
Propionyl-CoA: 14.9
Acetyl-CoA: 12.8
fatty_acid: 7.5
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (4402..4694): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 224.1
Ethylmalonyl-CoA: 222.4
Methoxymalonyl-CoA: 181.8
2-Methylbutyryl-CoA: 147.5
trans-1,2-CPDA: 134.2
Propionyl-CoA: 115.7
Malonyl-CoA: 101.8
CHC-CoA: 95.7
Isobutyryl-CoA: 92.8
Benzoyl-CoA: 74.8
Acetyl-CoA: 56.6
3-Methylbutyryl-CoA: 39.4
fatty_acid: 37.5
inactive: 33.0

nemA4: mal - mmal - mal

PKS_AT (578..876): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 204.9
inactive: 91.6
Methoxymalonyl-CoA: 53.2
Methylmalonyl-CoA: 45.6
Acetyl-CoA: 25.4
Ethylmalonyl-CoA: 24.8
Propionyl-CoA: 23.4
Benzoyl-CoA: 19.0
Isobutyryl-CoA: 15.1
2-Methylbutyryl-CoA: 14.2
CHC-CoA: 13.7
fatty_acid: 11.4
trans-1,2-CPDA: 8.4
3-Methylbutyryl-CoA: 0.0

PKS_AT (2105..2403): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 193.1
Ethylmalonyl-CoA: 188.7
Methoxymalonyl-CoA: 164.4
2-Methylbutyryl-CoA: 139.0
trans-1,2-CPDA: 118.9
Malonyl-CoA: 102.7
Propionyl-CoA: 93.7
CHC-CoA: 93.2
Isobutyryl-CoA: 87.9
Benzoyl-CoA: 64.4
Acetyl-CoA: 51.2
3-Methylbutyryl-CoA: 43.8
fatty_acid: 43.0
inactive: 35.5

PKS_AT (3631..3931): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 62.5%; CE-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 196.2
Methylmalonyl-CoA: 72.5
Methoxymalonyl-CoA: 69.5
inactive: 62.9
Propionyl-CoA: 44.9
Ethylmalonyl-CoA: 32.2
Acetyl-CoA: 29.4
Isobutyryl-CoA: 27.9
fatty_acid: 23.4
trans-1,2-CPDA: 20.7
CHC-CoA: 18.0
2-Methylbutyryl-CoA: 17.8
Benzoyl-CoA: 17.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor