BGC0000110 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000110 (Streptomyces orinoci)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	38,365	High	spectinabilin/orinocin/SNF4435C/SNF4435D	PKS:Type I

BGC0000110 - Region 1 - T1PKS

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Location: 1 - 38,365 nt. (total: 38,365 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 38,366
Label		BGC0000110

Tailoring functions

Oxidoreductases: oxygenase

norH: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acetyltransferase

norA_: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

Isomerases: isomerase

norG: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase

norA: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

norB: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

norC: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

norE: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000110.5	0.99	PKS	spectinabilin, orinocin, SNF4435C, SNF4435D	Streptomyces orinoci
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000024.5	0.69	PKS	aureothin	Streptomyces thioluteus
BGC0000041.3	0.68	PKS	oronofacic acid	Pseudomonas syringae
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002175.3	0.66	PKS	YWA1	Aspergillus oryzae RIB40

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000110.5	0.99	PKS	spectinabilin, orinocin, SNF4435C, SNF4435D	Streptomyces orinoci
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000024.5	0.69	PKS	aureothin	Streptomyces thioluteus
BGC0000041.3	0.68	PKS	oronofacic acid	Pseudomonas syringae
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002175.3	0.66	PKS	YWA1	Aspergillus oryzae RIB40

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 38365:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (Me-ccmal - Me-ccmal) + (redmal) + (pk - mal)
Non-colinear ordering:: norA + norA_ + norB + norC

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

norE: X

AMP-binding (127..513): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: NGAFVAVLLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

norA: mmal

PKS_AT (644..942): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 221.5
Ethylmalonyl-CoA: 219.7
Methoxymalonyl-CoA: 184.4
2-Methylbutyryl-CoA: 151.0
trans-1,2-CPDA: 137.5
Propionyl-CoA: 127.1
Malonyl-CoA: 121.8
CHC-CoA: 109.1
Isobutyryl-CoA: 82.5
Benzoyl-CoA: 74.9
Acetyl-CoA: 49.8
fatty_acid: 45.8
3-Methylbutyryl-CoA: 35.7
inactive: 30.7

norA_: mmal - mmal

PKS_AT (564..862): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 221.1
Ethylmalonyl-CoA: 214.4
Methoxymalonyl-CoA: 181.5
2-Methylbutyryl-CoA: 118.6
trans-1,2-CPDA: 115.5
Malonyl-CoA: 110.3
CHC-CoA: 107.3
Propionyl-CoA: 102.8
Isobutyryl-CoA: 95.4
Benzoyl-CoA: 73.9
Acetyl-CoA: 59.2
3-Methylbutyryl-CoA: 44.4
fatty_acid: 33.5
inactive: 30.6

PKS_AT (2337..2636): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 217.4
Ethylmalonyl-CoA: 211.3
Methoxymalonyl-CoA: 174.0
2-Methylbutyryl-CoA: 147.7
trans-1,2-CPDA: 128.7
Propionyl-CoA: 110.7
Malonyl-CoA: 104.1
CHC-CoA: 103.2
Benzoyl-CoA: 92.8
Isobutyryl-CoA: 92.2
Acetyl-CoA: 50.1
fatty_acid: 28.4
inactive: 27.0
3-Methylbutyryl-CoA: 21.7

norB: mal

PKS_AT (567..865): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.8
inactive: 103.3
Methoxymalonyl-CoA: 55.1
Methylmalonyl-CoA: 37.8
Propionyl-CoA: 29.1
Acetyl-CoA: 17.1
Isobutyryl-CoA: 16.2
Ethylmalonyl-CoA: 14.3
Benzoyl-CoA: 9.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.2

norC: pk - mal

PKS_AT (578..876): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 218.7
Methylmalonyl-CoA: 215.4
Methoxymalonyl-CoA: 182.7
2-Methylbutyryl-CoA: 131.1
trans-1,2-CPDA: 128.6
Propionyl-CoA: 113.3
Malonyl-CoA: 101.6
CHC-CoA: 97.8
Isobutyryl-CoA: 94.5
Benzoyl-CoA: 75.4
Acetyl-CoA: 64.0
fatty_acid: 42.5
3-Methylbutyryl-CoA: 41.3
inactive: 36.8

PKS_AT (1608..1778): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 20.6
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor