BGC0000110 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000110 (Streptomyces orinoci)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	38,365	spectinabilin/orinocin/SNF4435C/SNF4435D	PKS:Type I	100%

BGC0000110 - Region 1 - T1PKS

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Location: 1 - 38,365 nt. (total: 38,365 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 38,366
Label		BGC0000110

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000110	1.00	Polyketide	spectinabilin, orinocin, SNF4435C, SNF4435D	Streptomyces orinoci
BGC0000024	0.39	Polyketide	aureothin	Streptomyces thioluteus
BGC0000041	0.36	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0002031	0.35	Alkaloid	kitasetaline	Kitasatospora setae KM-6054
BGC0002405	0.34	Saccharide	mid-chain acyl sugars	Solanum lycopersicum
BGC0001068	0.30	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0002171	0.30	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275	0.30	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger CBS 101883
BGC0001276	0.29	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273	0.29	Polyketide	asperlactone	Aspergillus ochraceus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000110	1.00	Polyketide	spectinabilin, orinocin, SNF4435C, SNF4435D	Streptomyces orinoci
BGC0000024	0.71	Polyketide	aureothin	Streptomyces thioluteus
BGC0000041	0.68	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0002031	0.68	Alkaloid	kitasetaline	Kitasatospora setae KM-6054
BGC0002405	0.67	Saccharide	mid-chain acyl sugars	Solanum lycopersicum
BGC0001068	0.63	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0002171	0.63	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275	0.63	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger CBS 101883
BGC0001276	0.63	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273	0.63	Polyketide	asperlactone	Aspergillus ochraceus

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 38365:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (Me-ccmal - Me-ccmal) + (redmal) + (pk - mal)
Non-colinear ordering:: norA + norA_ + norB + norC

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

norE

AMP-binding (127..513): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: NGAFVAVLLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

norA

PKS_AT (644..942): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 221.5
Ethylmalonyl-CoA: 219.7
Methoxymalonyl-CoA: 184.4
2-Methylbutyryl-CoA: 151.0
trans-1,2-CPDA: 137.5
Propionyl-CoA: 127.1
Malonyl-CoA: 121.8
CHC-CoA: 109.1
Isobutyryl-CoA: 82.5
Benzoyl-CoA: 74.9
Acetyl-CoA: 49.8
fatty_acid: 45.8
3-Methylbutyryl-CoA: 35.7
inactive: 30.7

norA_

PKS_AT (564..862): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 221.1
Ethylmalonyl-CoA: 214.4
Methoxymalonyl-CoA: 181.5
2-Methylbutyryl-CoA: 118.6
trans-1,2-CPDA: 115.5
Malonyl-CoA: 110.3
CHC-CoA: 107.3
Propionyl-CoA: 102.8
Isobutyryl-CoA: 95.4
Benzoyl-CoA: 73.9
Acetyl-CoA: 59.2
3-Methylbutyryl-CoA: 44.4
fatty_acid: 33.5
inactive: 30.6

PKS_AT (2337..2636): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 217.4
Ethylmalonyl-CoA: 211.3
Methoxymalonyl-CoA: 174.0
2-Methylbutyryl-CoA: 147.7
trans-1,2-CPDA: 128.7
Propionyl-CoA: 110.7
Malonyl-CoA: 104.1
CHC-CoA: 103.2
Benzoyl-CoA: 92.8
Isobutyryl-CoA: 92.2
Acetyl-CoA: 50.1
fatty_acid: 28.4
inactive: 27.0
3-Methylbutyryl-CoA: 21.7

norB

PKS_AT (567..865): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.8
inactive: 103.3
Methoxymalonyl-CoA: 55.1
Methylmalonyl-CoA: 37.8
Propionyl-CoA: 29.1
Acetyl-CoA: 17.1
Isobutyryl-CoA: 16.2
Ethylmalonyl-CoA: 14.3
Benzoyl-CoA: 9.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.2

norC

PKS_AT (578..876): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 218.7
Methylmalonyl-CoA: 215.4
Methoxymalonyl-CoA: 182.7
2-Methylbutyryl-CoA: 131.1
trans-1,2-CPDA: 128.6
Propionyl-CoA: 113.3
Malonyl-CoA: 101.6
CHC-CoA: 97.8
Isobutyryl-CoA: 94.5
Benzoyl-CoA: 75.4
Acetyl-CoA: 64.0
fatty_acid: 42.5
3-Methylbutyryl-CoA: 41.3
inactive: 36.8

PKS_AT (1608..1778): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 20.6
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0