BGC0000112: neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus subsp. neocarzinostaticus
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Location: 1 - 78,477 nt. (total: 78,477 nt).
This entry is originally from NCBI GenBank AY117439.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
KnownClusterBlast
NRPS/PKS domains
General information about the BGC
MIBiG accession BGC0000112
Short description neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus subsp. neocarzinostaticus
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • PKS (Iterative type I)
Loci
AY117439.1
10097 - 72712
via Knock-out studies, Enzymatic assays, Gene expression correlated with compound production
Compounds
  • neocarzinostatin
Species Streptomyces carzinostaticus subsp. neocarzinostaticus [taxonomy]
References
Chemical products information
neocarzinostatin Evidence:
Copy SMILES
C35H33N1O12
Chemical database entries
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AAM77980.1
 10097 - 10894 (+) NDP-hexose-4-ketoreductase
  • Tailoring
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  • AAM77981.1
 11170 - 12750 (+) aminotransferase
  • Tailoring
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  • AAM77982.1
 12823 - 13503 (+) Unknown
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    • AAM77983.1
    		 13500 - 14543 (+) Unknown
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      • AAM77984.1
      		 14674 - 15672 (+) O-methyltransferase
      • Tailoring
      • Activity assay
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      • AAM77985.1
      		 15786 - 16220 (+) Unknown
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        • AAM77986.1
        		 16754 - 22015 (-) iterative type I polyketide synthase
        • Scaffold biosynthesis
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        • AAM77987.1
        		 22479 - 24155 (+) adenylate ligase
        • Activation / processing
        • Activity assay
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        • AAM77988.1
        		 24215 - 26377 (-) membrane protein
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          • AAM77989.1
          		 26475 - 27179 (-) NDP-hexose synthetase
          • Activation / processing
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          • AAM77990.1
          		 27203 - 28198 (-) NDP-hexose-4,6-dehydratase
          • Tailoring
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          • AAM77991.1
          		 28341 - 29060 (-) N-methyltransferase
          • Tailoring
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          • AAM77992.1
          		 29285 - 30493 (+) glycosyltransferase
          • Tailoring
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          • AAM77993.1
          		 30490 - 31383 (+) Unknown
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            • AAM77994.1
            		 31475 - 31918 (-) neocarzinostatin apoprotein
            • Resistance/immunity
            • Activity assay
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            • AAM77995.1
            		 32765 - 33922 (+) epoxide hydrolase
            • Tailoring
            • Activity assay
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            • AAM77996.1
            		 34420 - 35190 (-) esterase/hydrolase
            • Tailoring
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            • AAM77997.1
            		 35390 - 36622 (-) cytochrome P-450
            • Tailoring
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            • AAM77998.1
            		 36937 - 37308 (-) hydroxylase
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              • AAM77999.1
              		 37422 - 38906 (-) transmembrane transporter
              • Transport
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              • AAM78000.1
              		 39459 - 40076 (+) transcriptional regulator
              • Regulation
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              • AAM78001.1
              		 40368 - 41744 (+) oxidase
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                • AAM78002.1
                		 41951 - 43114 (-) epoxide hydrolase
                • Tailoring
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                • AAM78003.1
                		 43345 - 44670 (+) hydroxylase
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                  • AAM78004.1
                  		 44775 - 45578 (-) Unknown
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                    • AAM78005.1
                    		 45715 - 47373 (-) oxidoreductase
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                      • AAM78006.1
                      		 47370 - 47969 (-) Unknown
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                        • AAM78007.1
                        		 48237 - 49301 (+) transcriptional regulator
                        • Regulation
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                        • AAM78008.1
                        		 49345 - 50139 (+) transcriptional regulator
                        • Regulation
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                        • AAM78009.1
                        		 50199 - 51551 (-) cytochrome P-450
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                          • AAM78010.1
                          		 51548 - 52096 (-) flavin reductase
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                            • AAM78011.1
                            		 52093 - 52554 (-) thioesterase
                            • Scaffold biosynthesis
                            • Activity assay
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                            • AAM78012.1
                            		 52544 - 58477 (-) warhead-forming iterative polyketide synthase
                            • Scaffold biosynthesis
                            • Activity assay
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                            • AAM78013.1
                            		 58474 - 59568 (-) putative transporter
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                              • AAM78014.1
                              		 59565 - 61475 (-) Unknown
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                                • AAM78015.1
                                		 61472 - 62458 (-) Unknown
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                                  • AAM78016.1
                                  		 62542 - 63522 (-) Unknown
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                                    • AAM78017.1
                                    		 63625 - 64068 (-) Unknown
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                                      • AAM78018.1
                                      		 64364 - 65566 (+) transposase
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                                        • AAM78019.1
                                        		 65682 - 66860 (-) transcriptional regulator
                                        • Regulation
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                                        • AAM78020.1
                                        		 67166 - 67786 (+) transcriptional regulator
                                        • Regulation
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                                        • AAM78021.1
                                        		 67831 - 68811 (-) NDP-hexose-2,3-dehydratase
                                        • Regulation
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                                        • AAM78022.1
                                        		 68808 - 69434 (-) butyrolactone receptor
                                        • Regulation
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                                        • AAM78023.1
                                        		 69554 - 70507 (+) transcriptional regulator
                                        • Regulation
                                        • Knock-out
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                                        • AAM78024.1
                                        		 70817 - 71611 (-) Unknown
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                                          • AAM78025.1
                                          		 71738 - 72712 (-) Unknown
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                                            Biosynthesis information

                                            Biosynthetic modules

                                            Name
                                            1
                                            Type
                                            pks-modular
                                            Genes
                                            AAM77986.1
                                            Substrates
                                            Integrated Monomers
                                            Domains
                                            acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
                                            Name
                                            2
                                            Type
                                            pks-modular
                                            Genes
                                            AAM78012.1
                                            Substrates
                                            Integrated Monomers
                                            Domains
                                            acyltransferase, ketosynthase, acyltransferase, ketoreductase, dehydratase, carrier (ACP), carrier (ACP)
                                            Annotation changelog

                                            Entry version: 4

                                            Date
                                            Changes
                                            Submitters
                                            Reviewers
                                            2022-10-07
                                            Update chemical activity to schema version 2.11
                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                            Entry version: 3

                                            Date
                                            Changes
                                            Submitters
                                            Reviewers
                                            2022-09-15
                                            Corrected incomplete and duplicated gene identifiers
                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                            Entry version: 2

                                            Date
                                            Changes
                                            Submitters
                                            Reviewers
                                            2019-10-16
                                            Migrated from v1.4
                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                            Entry version: 1

                                            Date
                                            Changes
                                            Submitters
                                            Reviewers
                                            2015-06-12
                                            Submitted
                                            • (ID: E2T2HCM4X7QXVS4LL4JVBPPK)
                                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
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