BGC0000112: neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus subsp. neocarzinostaticus
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Location: 10,097 - 72,712 nt. (total: 62,616 nt).
This entry is originally from NCBI GenBank AY117439.1.

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General
Compounds
Genes
Polyketide
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NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000112
Short description neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus subsp. neocarzinostaticus
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • Polyketide (Enediyine)
Loci NCBI GenBank: AY117439.1
Compounds
  • neocarzinostatin
Species Streptomyces carzinostaticus subsp. neocarzinostaticus [taxonomy]
References
Chemical products information
neocarzinostatin [synonyms: neocarzinostatin chromophore]
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C35H33N1O12
Chemical database entries
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AAM77980.1
 10097 - 10894 (+) NDP-hexose-4-ketoreductase
  • Tailoring (Reduction)
Sequence-based prediction
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  • AAM77981.1
 11170 - 12750 (+) aminotransferase
  • Tailoring (Amination)
Sequence-based prediction
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  • AAM77982.1
 12823 - 13503 (+) Unknown
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  • AAM77983.1
 13500 - 14543 (+) Unknown
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  • AAM77984.1
 14674 - 15672 (+) O-methyltransferase
  • Tailoring (Methylation)
Activity assay
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  • AAM77985.1
 15786 - 16220 (+) Unknown
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  • AAM77986.1
 16754 - 22015 (-) iterative type I polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AAM77987.1
 22479 - 24155 (+) adenylate ligase
  • Activation / processing
Activity assay
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  • AAM77988.1
 24215 - 26377 (-) membrane protein
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  • AAM77989.1
 26475 - 27179 (-) NDP-hexose synthetase
  • Activation / processing
Sequence-based prediction
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  • AAM77990.1
 27203 - 28198 (-) NDP-hexose-4,6-dehydratase
  • Tailoring (Dehydration)
Sequence-based prediction
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  • AAM77991.1
 28341 - 29060 (-) N-methyltransferase
  • Tailoring (Amination)
Sequence-based prediction
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  • AAM77992.1
 29285 - 30493 (+) glycosyltransferase
  • Tailoring (Glycosylation)
Sequence-based prediction
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  • AAM77993.1
 30490 - 31383 (+) Unknown
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  • AAM77994.1
 31475 - 31918 (-) neocarzinostatin apoprotein
  • Resistance/immunity
Activity assay
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  • AAM77995.1
 32765 - 33922 (+) epoxide hydrolase
  • Tailoring (Hydrolysis)
Activity assay
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  • AAM77996.1
 34420 - 35190 (-) esterase/hydrolase
  • Tailoring (Acylation)
Sequence-based prediction
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  • AAM77997.1
 35390 - 36622 (-) cytochrome P-450
  • Tailoring (Hydroxylation)
Sequence-based prediction
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  • AAM77998.1
 36937 - 37308 (-) hydroxylase
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  • AAM77999.1
 37422 - 38906 (-) transmembrane transporter
  • Transport
Sequence-based prediction
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  • AAM78000.1
 39459 - 40076 (+) transcriptional regulator
  • Regulation
Sequence-based prediction
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  • AAM78001.1
 40368 - 41744 (+) oxidase
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  • AAM78002.1
 41951 - 43114 (-) epoxide hydrolase
  • Tailoring (Hydrolysis)
Activity assay
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  • AAM78003.1
 43345 - 44670 (+) hydroxylase
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  • AAM78004.1
 44775 - 45578 (-) Unknown
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  • AAM78005.1
 45715 - 47373 (-) oxidoreductase
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  • AAM78006.1
 47370 - 47969 (-) Unknown
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  • AAM78007.1
 48237 - 49301 (+) transcriptional regulator
  • Regulation
Sequence-based prediction
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  • AAM78008.1
 49345 - 50139 (+) transcriptional regulator
  • Regulation
Sequence-based prediction
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  • AAM78009.1
 50199 - 51551 (-) cytochrome P-450
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  • AAM78010.1
 51548 - 52096 (-) flavin reductase
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  • AAM78011.1
 52093 - 52554 (-) thioesterase
  • Scaffold biosynthesis
Activity assay
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  • AAM78012.1
 52544 - 58477 (-) warhead-forming iterative polyketide synthase
  • Scaffold biosynthesis
Activity assay
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  • AAM78013.1
 58474 - 59568 (-) putative transporter
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  • AAM78014.1
 59565 - 61475 (-) Unknown
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  • AAM78015.1
 61472 - 62458 (-) Unknown
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  • AAM78016.1
 62542 - 63522 (-) Unknown
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  • AAM78017.1
 63625 - 64068 (-) Unknown
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  • AAM78018.1
 64364 - 65566 (+) transposase
  • Unknown
Sequence-based prediction
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  • AAM78019.1
 65682 - 66860 (-) transcriptional regulator
  • Regulation
Sequence-based prediction
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  • AAM78020.1
 67166 - 67786 (+) transcriptional regulator
  • Regulation
Sequence-based prediction
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  • AAM78021.1
 67831 - 68811 (-) NDP-hexose-2,3-dehydratase
  • Regulation
Sequence-based prediction
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  • AAM78022.1
 68808 - 69434 (-) butyrolactone receptor
  • Regulation
Sequence-based prediction
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  • AAM78023.1
 69554 - 70507 (+) transcriptional regulator
  • Regulation
Knock-out
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  • AAM78024.1
 70817 - 71611 (-) Unknown
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  • AAM78025.1
 71738 - 72712 (-) Unknown
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Polyketide-specific information
Subclass Enediyine
Starter unit Acetyl-CoA
Cyclic? no
Polyketide-synthases
Genes Properties Modules
AAM78011.1
+
AAM77986.1
+
AAM78012.1 		Synthase subclass: Iterative type I/Enediyne type I
Thioesterases:
  • AAM78011.1 (Type II)
Iterative PKS type: Partially reducing, Unknown
Iteration counts: 7
Module 1
Specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: AAM77986.1
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Non-canonical activity:
  • Iterated
Evidence for non-canonical activity:
  • Structure-based inference
Module 2
Specificity: Malonyl-CoA
Evidence for specificity: Activity assay
Genes: AAM78012.1
Core domains: Ketosynthase, Acyltransferase, Thiolation (ACP/PCP), Ketoreductase, Dehydratase, Phosphopantetheinyl transferase
KR-domain stereochemistry: L-OH
Non-canonical activity:
  • Iterated
Evidence for non-canonical activity:
  • Activity assay
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: E2T2HCM4X7QXVS4LL4JVBPPK, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected incomplete and duplicated gene identifiers
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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