Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000115.1:
Cluster 1Polyketide / Saccharide1123580

BGC0000115, nystatin biosynthetic gene cluster from Streptomyces noursei. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: nystatin A1
PubChem ID: 11286230
SMILES string: Copy to clipboard
Molecular formula: C47H75NO17
Average molecular mass: 926.107 Da
Molecular activity: Antifungal
Molecular target: cellular membrane/ergosterol

Class-specific details

Biosynthetic class(es):
Polyketide / Saccharide
Polyketide subclass:
Polyene (cyclic)
Polyketide synthase subclass:
Modular type I
Starter unit:
Malonyl-CoA
Thioesterase type:
Type I & II
Release / cyclization type:
Macrolactonization

Modular polyketide synthases:
nysI (AAF71766.1)
Module 10
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Scaffold-modifying domain: None
Module 11
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Scaffold-modifying domain: None
Module 12
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 13
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 14
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 15
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None

nysJ (AAF71767.1)
Module 16
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 17
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 18
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None

nysK (AAF71768.1)
Module 19
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: None

nysA (AAF71774.1)
Module 1
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Scaffold-modifying domain: None
Module is non-elongating
Evidence for non-elongation: Unknown

nysB (AAF71775.1)
Module 2
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Scaffold-modifying domain: None
Module 3
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Scaffold-modifying domain: None

nysC (AAF71776.1)
Module 4
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 5
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 6
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 7
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 8
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module 9
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Scaffold-modifying domain: None

Saccharide subclass:
hyrbid/tailoring
Glycosyltransferases:
NYSDI:
Specificity: mycoamine
Evidence for specificity: Unknown

Gene cluster description

nystatin (BGC0000115). Gene Cluster 1. Biosynthetic class = Polyketide/Saccharide. GenBank AF263912. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies
MIxS-compliance:Unknown
Contact for this cluster:Sergey Zotchev (Norwegian University of Science and Technology)

Literature references

1. Brautaset T et al. (2000) Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway. Chem Biol 7(6):395-403.