BGC0000116 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000116 (Pseudonocardia autotrophica)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	125,717	High	nystatin-like Pseudonocardia polyene A1	PKS

BGC0000116 - Region 1 - T1PKS

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Location: 1 - 125,717 nt. (total: 125,717 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 125,718
Label		BGC0000116

Tailoring functions

Oxidoreductases: oxygenase

cppL: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

cppN: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: PPTase acylase aminotransferase glycosyltransferase transferase

cppB: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

cppDI: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

cppDII: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

cppF: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1060: 4'-phosphopantetheinyl transferase

cppO: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

Hydrolases: esterase

cppE: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

ABV83220.1: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

cppA: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

cppC: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

cppI: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

cppJ: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

cppK: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000116.5	1.00	PKS	nystatin-like Pseudonocardia polyene A1	Pseudonocardia autotrophica
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000041.3	0.76	PKS	oronofacic acid	Pseudomonas syringae
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001709.5	0.73	PKS	nystatin	Streptomyces albulus
BGC0000115.5	0.69	PKS, saccharide	nystatin A1	Streptomyces noursei ATCC 11455
BGC0002175.3	0.66	PKS	YWA1	Aspergillus oryzae RIB40
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000116.5	1.00	PKS	nystatin-like Pseudonocardia polyene A1	Pseudonocardia autotrophica
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000041.3	0.76	PKS	oronofacic acid	Pseudomonas syringae
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001709.5	0.73	PKS	nystatin	Streptomyces albulus
BGC0000115.5	0.69	PKS, saccharide	nystatin A1	Streptomyces noursei ATCC 11455
BGC0002175.3	0.66	PKS	YWA1	Aspergillus oryzae RIB40
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 125717:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (Me-ohmal - Me-ohmal) + (ccmal - ccmal - redmal - ccmal - ccmal - ccmal) + (ccmal - ohmal - Me-ohmal - ohmal - ohmal - ohmal) + (redmal - ohmal - ccmal) + (ccmal)
Non-colinear ordering:: cppA + cppB + cppC + cppI + cppJ + cppK

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

cppI: mal - mal - mmal - mal - mal - mal

PKS_AT (567..874): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 206.0
inactive: 96.6
Methoxymalonyl-CoA: 60.5
Methylmalonyl-CoA: 51.7
Benzoyl-CoA: 26.0
Isobutyryl-CoA: 21.3
Propionyl-CoA: 21.0
Acetyl-CoA: 18.3
trans-1,2-CPDA: 16.7
CHC-CoA: 14.9
2-Methylbutyryl-CoA: 14.0
fatty_acid: 12.6
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -1.5

PKS_AT (2325..2621): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.7
inactive: 109.6
Methoxymalonyl-CoA: 64.4
Methylmalonyl-CoA: 55.0
Propionyl-CoA: 30.8
Ethylmalonyl-CoA: 29.2
Acetyl-CoA: 25.0
Isobutyryl-CoA: 22.6
Benzoyl-CoA: 20.3
CHC-CoA: 20.2
trans-1,2-CPDA: 16.8
2-Methylbutyryl-CoA: 16.8
fatty_acid: 14.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (3872..4169): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.2
Ethylmalonyl-CoA: 206.6
Methoxymalonyl-CoA: 180.1
2-Methylbutyryl-CoA: 133.9
trans-1,2-CPDA: 132.5
Propionyl-CoA: 118.7
Malonyl-CoA: 109.2
CHC-CoA: 101.9
Isobutyryl-CoA: 93.8
Benzoyl-CoA: 84.3
Acetyl-CoA: 61.9
fatty_acid: 37.7
inactive: 37.0
3-Methylbutyryl-CoA: 36.4

PKS_AT (5413..5711): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 226.2
inactive: 111.9
Methoxymalonyl-CoA: 76.1
Methylmalonyl-CoA: 66.2
Isobutyryl-CoA: 34.4
Propionyl-CoA: 25.2
Ethylmalonyl-CoA: 20.4
Benzoyl-CoA: 19.9
fatty_acid: 14.5
Acetyl-CoA: 14.4
CHC-CoA: 13.3
trans-1,2-CPDA: 10.3
2-Methylbutyryl-CoA: 8.6
3-Methylbutyryl-CoA: 0.0

PKS_AT (6929..7228): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 206.0
inactive: 95.5
Methoxymalonyl-CoA: 66.5
Methylmalonyl-CoA: 61.4
Ethylmalonyl-CoA: 29.2
Isobutyryl-CoA: 23.9
Propionyl-CoA: 22.1
Benzoyl-CoA: 17.8
CHC-CoA: 16.2
Acetyl-CoA: 15.7
fatty_acid: 14.8
2-Methylbutyryl-CoA: 9.3
trans-1,2-CPDA: 8.0
3-Methylbutyryl-CoA: 5.3

PKS_AT (8437..8726): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 236.6
inactive: 112.1
Methoxymalonyl-CoA: 67.3
Methylmalonyl-CoA: 61.4
Propionyl-CoA: 36.5
Benzoyl-CoA: 29.1
Ethylmalonyl-CoA: 28.7
Isobutyryl-CoA: 27.2
Acetyl-CoA: 23.9
CHC-CoA: 19.2
fatty_acid: 15.2
trans-1,2-CPDA: 14.9
2-Methylbutyryl-CoA: 13.1
3-Methylbutyryl-CoA: 0.0

cppJ: mal - mal - mal

PKS_AT (573..851): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 223.7
inactive: 103.6
Methoxymalonyl-CoA: 68.5
Methylmalonyl-CoA: 64.1
Propionyl-CoA: 28.1
Isobutyryl-CoA: 27.6
Ethylmalonyl-CoA: 27.3
Benzoyl-CoA: 24.5
fatty_acid: 18.0
Acetyl-CoA: 17.8
CHC-CoA: 17.1
trans-1,2-CPDA: 15.6
2-Methylbutyryl-CoA: 9.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (2626..2918): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 193.8
inactive: 80.8
Methoxymalonyl-CoA: 50.8
Methylmalonyl-CoA: 44.3
Isobutyryl-CoA: 23.9
Propionyl-CoA: 21.5
Acetyl-CoA: 17.7
CHC-CoA: 17.2
Ethylmalonyl-CoA: 16.4
fatty_acid: 13.2
Benzoyl-CoA: 12.4
trans-1,2-CPDA: 8.1
2-Methylbutyryl-CoA: 0.6
3-Methylbutyryl-CoA: 0.0

PKS_AT (4149..4433): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Propionyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 201.8
inactive: 85.6
Methoxymalonyl-CoA: 73.0
Methylmalonyl-CoA: 69.0
Isobutyryl-CoA: 40.7
fatty_acid: 35.4
Propionyl-CoA: 33.8
Ethylmalonyl-CoA: 30.3
CHC-CoA: 26.5
Acetyl-CoA: 17.4
trans-1,2-CPDA: 13.4
Benzoyl-CoA: 10.5
2-Methylbutyryl-CoA: 9.1
3-Methylbutyryl-CoA: 3.6

cppK: mal

PKS_AT (553..841): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 202.3
inactive: 102.6
Methoxymalonyl-CoA: 62.3
Methylmalonyl-CoA: 44.0
Isobutyryl-CoA: 24.9
Propionyl-CoA: 21.4
Ethylmalonyl-CoA: 18.5
Acetyl-CoA: 15.1
Benzoyl-CoA: 12.9
CHC-CoA: 11.5
trans-1,2-CPDA: 5.4
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

cppA: mal

PKS_AT (523..818): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.1
inactive: 102.5
Methoxymalonyl-CoA: 65.9
Methylmalonyl-CoA: 62.4
Isobutyryl-CoA: 32.8
Ethylmalonyl-CoA: 32.7
Propionyl-CoA: 25.7
CHC-CoA: 19.2
Acetyl-CoA: 19.1
Benzoyl-CoA: 19.0
trans-1,2-CPDA: 13.0
fatty_acid: 12.5
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.8

cppB: mmal - mmal

PKS_AT (564..861): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 199.1
Ethylmalonyl-CoA: 187.2
Methoxymalonyl-CoA: 166.1
trans-1,2-CPDA: 123.6
2-Methylbutyryl-CoA: 117.1
Malonyl-CoA: 98.8
Propionyl-CoA: 93.4
CHC-CoA: 72.5
Isobutyryl-CoA: 71.7
Benzoyl-CoA: 57.2
Acetyl-CoA: 35.9
fatty_acid: 31.6
3-Methylbutyryl-CoA: 30.1
inactive: 22.3

PKS_AT (2097..2401): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 213.8
Ethylmalonyl-CoA: 208.6
Methoxymalonyl-CoA: 180.6
trans-1,2-CPDA: 139.5
2-Methylbutyryl-CoA: 139.1
Propionyl-CoA: 116.3
CHC-CoA: 114.8
Malonyl-CoA: 107.4
Benzoyl-CoA: 78.7
Isobutyryl-CoA: 77.7
Acetyl-CoA: 53.4
fatty_acid: 51.4
inactive: 35.0
3-Methylbutyryl-CoA: 30.2

cppC: mal - mal - mal - mal - mal - mal

PKS_AT (544..838): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.5
inactive: 95.8
Methoxymalonyl-CoA: 60.7
Methylmalonyl-CoA: 52.7
Propionyl-CoA: 29.5
Ethylmalonyl-CoA: 27.9
Isobutyryl-CoA: 23.3
CHC-CoA: 18.3
Acetyl-CoA: 17.1
Benzoyl-CoA: 15.1
trans-1,2-CPDA: 10.6
2-Methylbutyryl-CoA: 8.0
fatty_acid: 7.2
3-Methylbutyryl-CoA: 0.0

PKS_AT (2308..2601): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 204.0
inactive: 87.2
Methoxymalonyl-CoA: 50.5
Methylmalonyl-CoA: 49.7
Propionyl-CoA: 29.0
Ethylmalonyl-CoA: 25.8
Isobutyryl-CoA: 22.0
Acetyl-CoA: 16.2
Benzoyl-CoA: 14.8
trans-1,2-CPDA: 10.5
2-Methylbutyryl-CoA: 9.3
fatty_acid: 6.8
3-Methylbutyryl-CoA: 0.0
CHC-CoA: -1.3

PKS_AT (4052..4342): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.6
inactive: 103.3
Methoxymalonyl-CoA: 60.8
Methylmalonyl-CoA: 55.2
Propionyl-CoA: 30.2
Ethylmalonyl-CoA: 23.8
Acetyl-CoA: 23.1
Isobutyryl-CoA: 19.8
Benzoyl-CoA: 17.7
CHC-CoA: 14.7
2-Methylbutyryl-CoA: 12.3
trans-1,2-CPDA: 9.1
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (6112..6404): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 214.4
inactive: 106.2
Methoxymalonyl-CoA: 59.3
Methylmalonyl-CoA: 57.2
Propionyl-CoA: 39.8
Acetyl-CoA: 22.9
Isobutyryl-CoA: 20.5
Ethylmalonyl-CoA: 19.1
Benzoyl-CoA: 13.9
CHC-CoA: 12.7
fatty_acid: 9.6
trans-1,2-CPDA: 8.7
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -3.2

PKS_AT (7847..8136): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 215.3
inactive: 94.3
Methoxymalonyl-CoA: 62.8
Methylmalonyl-CoA: 59.0
Propionyl-CoA: 31.8
Ethylmalonyl-CoA: 27.1
Isobutyryl-CoA: 24.9
Acetyl-CoA: 19.2
2-Methylbutyryl-CoA: 17.6
CHC-CoA: 16.3
Benzoyl-CoA: 12.3
fatty_acid: 11.9
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (9575..9873): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 232.5
inactive: 110.2
Methoxymalonyl-CoA: 57.9
Methylmalonyl-CoA: 55.2
Propionyl-CoA: 35.5
Ethylmalonyl-CoA: 29.5
Acetyl-CoA: 27.4
CHC-CoA: 24.4
Benzoyl-CoA: 23.7
trans-1,2-CPDA: 21.8
Isobutyryl-CoA: 16.6
2-Methylbutyryl-CoA: 15.5
fatty_acid: 10.4
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor