BGC0000119

BGC0000119: 7-deoxypactamycin biosynthetic gene cluster from Streptomyces pactum

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Location: 1 - 86,350 nt. (total: 86,350 nt).
This entry is originally from NCBI GenBank FJ392609.1.

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core biosynthetic genes

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Gene details

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General

Compounds

Genes

Biosynthesis

History

NRPS/PKS domains

KnownClusterBlast

General information about the BGC

MIBiG accession	BGC0000119
Short description	7-deoxypactamycin biosynthetic gene cluster from Streptomyces pactum
Status	Quality: questionable The quality level of this entry. Status: active The status of this entry. Completeness: complete Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)	PKS (Iterative type I) saccharide (hybrid/tailoring)
Loci	FJ392609.1 via Knock-out studies, Enzymatic assays, Heterologous expression
Compounds	7-deoxypactamycin
Species	Streptomyces pactum [taxonomy]
References	[1] Cloning of the pactamycin biosynthetic gene cluster and characterization of a crucial glycosyltransferase prior to a unique cyclopentane ring formation. Kudo F et al., J Antibiot (Tokyo) (2007) PMID:17827660 [2] Deciphering pactamycin biosynthesis and engineered production of new pactamycin analogues. Ito T et al., Chembiochem (2009) PMID:19670201 [3] Biosynthetic studies and genetic engineering of pactamycin analogs with improved selectivity toward malarial parasites. Lu W et al., Chem Biol (2011) PMID:21513878 [4] Mutasynthesis of fluorinated pactamycin analogues and their antimalarial activity. Almabruk KH et al., Org Lett (2013) PMID:23521145 [5] A single PLP-dependent enzyme PctV catalyzes the transformation of 3-dehydroshikimate into 3-aminobenzoate in the biosynthesis of pactamycin. Hirayama A et al., Chembiochem (2013) PMID:23744829

Chemical products information

7-deoxypactamycin Evidence:

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C₂₈H₃₈N₄O₇

Chemical database entries
NPAtlas
PubCHEM

List of genes involved in compound(s) production

Identifiers	Position	Product	Functions	Evidence	Extra
ACJ24841.1	2 - 1489 (+)	phosphoribosylformylglycinamidine synthase II-like protein			copy AA seq copy Nt seq
ACJ24842.1	1756 - 2643 (+)	hypothetical protein			copy AA seq copy Nt seq
ACJ24843.1	2803 - 4377 (-)	n-acetylgalactosamine-6-sulfate sulfatase-like protein			copy AA seq copy Nt seq
ACJ24844.1	5443 - 7026 (+)	amidophosphoribosyltransferase-like protein			copy AA seq copy Nt seq
ACJ24845.1	7060 - 8160 (+)	phosphoribosylaminoimidazole synthetase-like protein			copy AA seq copy Nt seq
ACJ24846.1	9066 - 10160 (-)	valine dehydrogenase (NADP+)-like protein			copy AA seq copy Nt seq
ACJ24847.1	10461 - 11279 (+)	hypothetical protein			copy AA seq copy Nt seq
ACJ24848.1	12500 - 13135 (-)	hypothetical protein			copy AA seq copy Nt seq
ACJ24849.1	14199 - 18185 (-)	ATP-dependent helicase-like protein			copy AA seq copy Nt seq
ACJ24850.1	18815 - 19801 (+)	integral membrane protein			copy AA seq copy Nt seq
ACJ24851.1	19889 - 21616 (+)	putative ECF-family RNA polymerase sigma factor			copy AA seq copy Nt seq
ACJ24852.1	21753 - 22910 (+)	putative FAD-dependent pyridine nucleotide-disulphide oxidoreductase			copy AA seq copy Nt seq
ACJ24853.1	23582 - 24052 (+)	hypothetical protein			copy AA seq copy Nt seq
ACJ24854.1	24165 - 24932 (+)	putative translation initiation factor IF-2			copy AA seq copy Nt seq
ACJ24855.1	25050 - 25502 (+)	putative translation initiation factor IF-2			copy AA seq copy Nt seq
ACJ24856.1	25602 - 27968 (+)	putative ATP-dependent RNA helicase			copy AA seq copy Nt seq
ACJ24857.1 ptmY	29893 - 31086 (-)	putative cytochrome P450 monooxygenase	Tailoring	Knock-out	copy AA seq copy Nt seq
ACJ24858.1 ptmZ	31140 - 31628 (-)	glutathione peroxidase-like protein			copy AA seq copy Nt seq
ACJ24859.1 ptmA	31845 - 33020 (-)	L-alanine:N-amidino-3-keto-scyllo-inosamine aminotransferase-like protein	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
ACJ24860.1 ptmB	33035 - 34747 (-)	putative carbamoyltransferase	Tailoring	Knock-out	copy AA seq copy Nt seq
ACJ24861.1 ptmC	35020 - 36123 (+)	Fe-S radical SAM oxidoreductase-like protein	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
ACJ24862.1 ptmD	36189 - 37265 (+)	HemK family methyltransferase	Tailoring	Knock-out	copy AA seq copy Nt seq
ACJ24863.1 ptmE	37258 - 37794 (-)	putative glucokinase			copy AA seq copy Nt seq
ACJ24864.1 ptmF	37794 - 38444 (-)	two component transcriptional regulator	Regulation		copy AA seq copy Nt seq
ACJ24865.1 ptmG	38620 - 39756 (-)	putative nucleotydyltransferase			copy AA seq copy Nt seq
ACJ24866.1 ptmH	40175 - 42160 (+)	putative C-methyltransferase	Tailoring	Knock-out	copy AA seq copy Nt seq
ACJ24867.1 ptmI	42192 - 42476 (+)	putative acyl carrier protein	Scaffold biosynthesis		copy AA seq copy Nt seq
ACJ24868.1 ptmJ	42482 - 43438 (+)	putative glycosyltransferase	Scaffold biosynthesis	Activity assay	copy AA seq copy Nt seq
ACJ24869.1 ptmK	43435 - 45123 (+)	putative ketoacyl-ACP synthase	Scaffold biosynthesis		copy AA seq copy Nt seq
ACJ24870.1 ptmL	45116 - 46843 (+)	putative C-methyltransferase	Tailoring	Knock-out	copy AA seq copy Nt seq
ACJ24871.1 ptmM	46894 - 48630 (+)	putative C-methyltransferase	Tailoring	Knock-out	copy AA seq copy Nt seq
ACJ24872.1 ptmN	48634 - 49689 (+)	putative NAD dependent dehydrogenase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
ACJ24873.1 ptmO	50000 - 50773 (-)	putative hydrolase/acyltransferase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
ACJ24874.1 ptmP	50883 - 51518 (+)	phosphopantetheinyl transferase	Activation / processing	Knock-out	copy AA seq copy Nt seq
ACJ24875.1 ptmQ	52474 - 58059 (-)	6-methylsalicylic acid synthase	Precursor biosynthesis	Knock-out	copy AA seq copy Nt seq
ACJ24876.1 ptmR	58056 - 59129 (-)	ketoacyl-ACP synthase	Precursor biosynthesis		copy AA seq copy Nt seq
ACJ24877.1 ptmS	59143 - 60660 (-)	putative AMP-forming acyl-CoA synthetase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
ACJ24878.1 ptmT	60657 - 61991 (-)	glutamate-1-semialdehyde aminotransferase-like protein	Precursor biosynthesis	Knock-out	copy AA seq copy Nt seq
ACJ24879.1 ptmU	62153 - 62992 (+)	putative short-chain dehydrogenase/reductase	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
ACJ24880.1 ptmV	63030 - 63710 (+)	putative phosphatase			copy AA seq copy Nt seq
ACJ24881.1 ptmW	64635 - 65450 (+)	hypothetical protein			copy AA seq copy Nt seq
ACJ24882.1 ptmX	66186 - 67481 (+)	integral membrane protein			copy AA seq copy Nt seq
ACJ24883.1	67855 - 69201 (+)	putative peptidase			copy AA seq copy Nt seq
ACJ24884.1	69778 - 70359 (+)	nourseothricin acetyltransferase-like protein			copy AA seq copy Nt seq
ACJ24885.1	70343 - 73474 (-)	putative ATP-dependent helicase			copy AA seq copy Nt seq
ACJ24886.1	73641 - 74723 (+)	hypothetical protein			copy AA seq copy Nt seq
ACJ24887.1	74737 - 76386 (-)	hypothetical protein			copy AA seq copy Nt seq
ACJ24888.1	76463 - 76867 (+)	glycosyl transferase family 51			copy AA seq copy Nt seq
ACJ24889.1	77327 - 78619 (-)	putative methyltransferase			copy AA seq copy Nt seq
ACJ24890.1	78767 - 79951 (-)	hypothetical protein			copy AA seq copy Nt seq
ACJ24891.1	80490 - 81968 (-)	sporulation associated protein			copy AA seq copy Nt seq
ACJ24892.1	82831 - 84993 (+)	putative secreted protein			copy AA seq copy Nt seq
ACJ24893.1	85154 - >86350 (+)	multi-domain regulatory protein			copy AA seq copy Nt seq

Biosynthesis information

Annotation changelog

Entry version: 4

Date

Changes

Submitters

Reviewers

2022-10-07

Update chemical activity to schema version 2.11

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 3

Date

Changes

Submitters

Reviewers

2022-09-15

Added version numbers to protein accessions

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

2022-09-15

Removed duplicate gene name

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

2022-09-15

Corrected gene identifiers

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

2022-09-15

Updated bioactivity data

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 2

Date

Changes

Submitters

Reviewers

2019-10-16

Migrated from v1.4

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

2019-10-16

Updated compound(s) information (NPAtlas curation)

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(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 1

Date

Changes

Submitters

Reviewers

2015-06-12

Submitted

(ID: ZV4PLWZMKXHKHM6T5Z6RFAE3)

(ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Detailed domain annotation

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