Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000119.1:
Cluster 1Polyketide / Other / Saccharide186350

BGC0000119, pactamycin biosynthetic gene cluster from Streptomyces pactum. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: pactamycin
PubChem ID: 5289124
SMILES string: Copy to clipboard
Molecular formula: C28H38N4O8
Average molecular mass: 558.632 Da
Molecular activity: Antibacterial, Antifungal, Cytotoxic
Molecular target: Ribosomes

Class-specific details

Biosynthetic class(es):
Polyketide / Other / Saccharide
Polyketide subclass:
Other (linear)
Polyketide synthase subclass:
Iterative type I
Starter unit:
Acetyl-CoA
Polyketide synthase / ketosynthase-encoding genes:
ACJ24875
Iterative PKS subtype:
Partially reducing
Number of iterations:
3
C2-C7 first
Thioesterase type:
None
Release / cyclization type:
Other

Saccharide subclass:
hybrid/tailoring
Glycosyltransferases:
PTMJ:
Specificity: Unknown
Evidence for specificity: Unknown
ACJ24888.1:
Specificity: Unknown
Evidence for specificity: Unknown
Other biosynthetic class:
None

Gene cluster description

pactamycin (BGC0000119). Gene Cluster 1. Biosynthetic class = Polyketide/Other/Saccharide. GenBank FJ392609. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Taifo Mahmud (Oregon State University)

Literature references

1. Kudo F et al. (2007) Cloning of the pactamycin biosynthetic gene cluster and characterization of a crucial glycosyltransferase prior to a unique cyclopentane ring formation. J Antibiot (Tokyo) 60(8):492-503. doi: 10.1038/ja.2007.63.
2. Ito T et al. (2009) Deciphering pactamycin biosynthesis and engineered production of new pactamycin analogues. Chembiochem 10(13):2253-65. doi: 10.1002/cbic.200900339.
3. Lu W et al. (2011) Biosynthetic studies and genetic engineering of pactamycin analogs with improved selectivity toward malarial parasites. Chem Biol 18(4):425-31. doi: 10.1016/j.chembiol.2011.01.016.
4. Almabruk KH et al. (2013) Mutasynthesis of fluorinated pactamycin analogues and their antimalarial activity. Org Lett 15(7):1678-81. doi: 10.1021/ol4004614. Epub 2013 Mar 22.
5. Hirayama A et al. (2013) A single PLP-dependent enzyme PctV catalyzes the transformation of 3-dehydroshikimate into 3-aminobenzoate in the biosynthesis of pactamycin. Chembiochem 14(10):1198-203. doi: 10.1002/cbic.201300153. Epub 2013