{"accession":"BGC0000120","version":5,"changelog":{"releases":[{"version":"1","entries":[{"contributors":["7M4BGWDEABIQBOJEBD7LO4YZ"],"reviewers":["AAAAAAAAAAAAAAAAAAAAAAAA"],"date":"2015-06-12","comment":"Submitted"}],"date":"2015-06-12"},{"version":"2","entries":[{"contributors":["AAAAAAAAAAAAAAAAAAAAAAAA"],"reviewers":["AAAAAAAAAAAAAAAAAAAAAAAA"],"date":"2019-10-16","comment":"Migrated from v1.4"},{"contributors":["AAAAAAAAAAAAAAAAAAAAAAAA"],"reviewers":["AAAAAAAAAAAAAAAAAAAAAAAA"],"date":"2019-10-16","comment":"Updated compound(s) information (MIBiG Annotathon)"},{"contributors":["AAAAAAAAAAAAAAAAAAAAAAAA"],"reviewers":["AAAAAAAAAAAAAAAAAAAAAAAA"],"date":"2019-10-16","comment":"Updated compound(s) information (NPAtlas curation)"}],"date":"2019-10-16"},{"version":"3","entries":[{"contributors":["AAAAAAAAAAAAAAAAAAAAAAAA"],"reviewers":["AAAAAAAAAAAAAAAAAAAAAAAA"],"date":"2022-09-15","comment":"Cleaned up synonym list"}],"date":"2022-09-15"},{"version":"4","entries":[{"contributors":["AAAAAAAAAAAAAAAAAAAAAAAA"],"reviewers":["AAAAAAAAAAAAAAAAAAAAAAAA"],"date":"2022-10-07","comment":"Update chemical activity to schema version 2.11"}],"date":"2022-10-07"}]},"quality":"questionable","status":"active","completeness":"complete","loci":[{"accession":"KF899892.1","location":{"from":0,"to":0},"evidence":[{"method":"Knock-out studies"},{"method":"Heterologous expression"},{"method":"Gene expression correlated with compound production"}]}],"biosynthesis":{"classes":[{"class":"PKS","subclass":"Iterative type I","cyclases":[]}],"modules":[{"type":"pks-modular","name":"1","genes":["AIG62146.1"],"active":true,"carriers":[{"type":"carrier","gene":"AIG62146.1","location":{"from":-1,"to":-1},"subtype":"ACP","beta_branching":false}],"modification_domains":[{"type":"dehydratase","gene":"AIG62146.1","location":{"from":-1,"to":-1}},{"type":"ketoreductase","gene":"AIG62146.1","location":{"from":-1,"to":-1}}],"at_domain":{"type":"acyltransferase","gene":"AIG62146.1","location":{"from":-1,"to":-1},"substrates":[],"evidence":[]},"ks_domain":{"type":"ketosynthase","gene":"AIG62146.1","location":{"from":-1,"to":-1}},"non_canonical_activity":{"evidence":[{"method":"Activity assay"}],"iterations":-1,"nonElongating":false,"skipped":false}}]},"compounds":[{"name":"patulin","evidence":[],"bioactivities":[{"name":"cytotoxic","observed":true,"references":[]}],"structure":"C1C=C2C(=CC(=O)O2)C(O1)O","mass":154.026608672,"formula":"C7H6O4"}],"taxonomy":{"name":"Penicillium expansum","ncbiTaxId":27334},"genes":{"annotations":[{"id":"AIG62144.1","name":"patH","product":"M-cresol hydroxylase","functions":[{"function":{"name":"Tailoring"},"evidence":[{"method":"Heterologous expression"}]}]},{"id":"AIG62133.1","name":"patG","product":"6-methylsalicylic decarboxylase","functions":[{"function":{"name":"Tailoring"},"evidence":[{"method":"Heterologous expression"}]}],"tailoring":[{"function":"Decarboxylation","references":["doi:10.1016/j.ijfoodmicro.2013.11.020"],"db_reference":"mite:MITE0000191","details":"Decarboxylase"}]},{"id":"AIG62135.1","name":"patD","product":"hypothetical protein","functions":[{"function":{"name":"Tailoring"},"evidence":[{"method":"Knock-out"}]}]},{"id":"AIG62143.1","name":"patL","product":"C6 transcription activator","functions":[{"function":{"name":"Regulation"},"evidence":[{"method":"Knock-out"}]}]},{"id":"AIG62145.1","name":"patI","product":"M-hydroxybenzyl alcohol hydroxylase","functions":[{"function":{"name":"Tailoring"},"evidence":[{"method":"Heterologous expression"}]}]}]},"comment":"The patulin genes cluster and its boudaries have been validated by the deletetion of patL (AIG62143), gene encoding the specific transcription factor (PMID: 26582186 ).\\n\\nThe patulin cluster has been described in A. clavatus (PMID:19383676)\\n\\nThe role of PatH (AIG62144) , PatI (AIG62145) and PatG (AIG62133) has been determined by heterologous expression studies (PMID:19383676 and PMID:24334092)\\n\\nThe role of PatE (AIG62134) has been determined by knock-out (27913847).","legacy_references":["pubmed:25120234","pubmed:26582186","pubmed:27913847","pubmed:24334092","pubmed:19383676"]}