BGC0000123 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000123 (Streptomyces sp. HK803)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	76,196	High	phoslactomycin B	PKS

BGC0000123 - Region 1 - T1PKS

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Location: 1 - 76,196 nt. (total: 76,196 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 76,197
Label		BGC0000123

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

chcA: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

plmL: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

plmM: NADH:flavin oxidoreductase/NADH oxidase

smcogs: SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase

plmS2: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

plmT4: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

plmT7: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

plmT8: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

Transferases: acylase aminotransferase

plm2-3: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

plm6: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

plmT1: aminotransferase

smcogs: SMCOG1019: aminotransferase

Hydrolases: esterase

plm8: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

plm1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

plm4: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

plm5: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

plm7: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

plmJK: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000123.5	1.00	PKS	phoslactomycin B	Streptomyces sp. HK803
BGC0001275.3	0.77	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000123.5	1.00	PKS	phoslactomycin B	Streptomyces sp. HK803
BGC0001275.3	0.77	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 76196:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - ccmal) + (ccmal - ohmal) + (ohemal) + (ccmal) + (ohemal) + (ohmal)
Non-colinear ordering:: plm1 + plm2-3 + plm4 + plm5 + plm6 + plm7

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

plm4: emal

PKS_AT (511..809): emal

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 100.0%; Methylmalonyl-CoA: 79.2%; Malonyl-CoA: 75.0%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 236.0
Methylmalonyl-CoA: 191.5
Methoxymalonyl-CoA: 153.7
trans-1,2-CPDA: 120.3
2-Methylbutyryl-CoA: 114.3
Propionyl-CoA: 96.0
Malonyl-CoA: 91.3
CHC-CoA: 84.6
Isobutyryl-CoA: 75.9
Benzoyl-CoA: 65.9
Acetyl-CoA: 45.7
inactive: 32.0
3-Methylbutyryl-CoA: 30.6
fatty_acid: 24.5

plm5: mal

PKS_AT (566..866): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 188.0
inactive: 106.2
Methoxymalonyl-CoA: 54.7
Methylmalonyl-CoA: 44.8
Propionyl-CoA: 35.8
Acetyl-CoA: 18.6
Ethylmalonyl-CoA: 17.4
Isobutyryl-CoA: 17.1
2-Methylbutyryl-CoA: 16.9
Benzoyl-CoA: 16.8
fatty_acid: 11.4
trans-1,2-CPDA: 8.5
CHC-CoA: 2.1
3-Methylbutyryl-CoA: 0.0

plm6: emal

PKS_AT (545..842): emal

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 100.0%; Methylmalonyl-CoA: 83.3%; Methoxymalonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 237.6
Methylmalonyl-CoA: 179.9
Methoxymalonyl-CoA: 154.8
trans-1,2-CPDA: 119.1
2-Methylbutyryl-CoA: 115.9
CHC-CoA: 107.0
Propionyl-CoA: 99.7
Malonyl-CoA: 87.1
Benzoyl-CoA: 77.6
Isobutyryl-CoA: 76.0
Acetyl-CoA: 50.6
3-Methylbutyryl-CoA: 31.9
fatty_acid: 31.7
inactive: 24.8

plm7: mal

PKS_AT (549..821): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 191.7
inactive: 89.6
Methylmalonyl-CoA: 78.5
Methoxymalonyl-CoA: 78.3
Ethylmalonyl-CoA: 44.6
Propionyl-CoA: 38.3
CHC-CoA: 34.0
2-Methylbutyryl-CoA: 32.3
Benzoyl-CoA: 30.2
Acetyl-CoA: 29.6
trans-1,2-CPDA: 28.9
fatty_acid: 21.1
Isobutyryl-CoA: 21.1
3-Methylbutyryl-CoA: 11.5

plm1: pk - mal

PKS_AT (202..495): pk

ATSignature: CHC-CoA

Top 3 matches:: CHC-CoA: 100.0%; Propionyl-CoA: 66.7%; Methylmalonyl-CoA: 66.7%

minowa: CHC-CoA

Prediction, score:

CHC-CoA: 271.0
Methylmalonyl-CoA: 147.7
Ethylmalonyl-CoA: 137.3
2-Methylbutyryl-CoA: 122.7
Methoxymalonyl-CoA: 120.3
Propionyl-CoA: 105.6
trans-1,2-CPDA: 94.0
Malonyl-CoA: 74.6
Isobutyryl-CoA: 57.8
Benzoyl-CoA: 46.3
fatty_acid: 27.0
Acetyl-CoA: 25.9
inactive: 15.7
3-Methylbutyryl-CoA: 13.1

PKS_AT (1187..1459): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 180.8
Methoxymalonyl-CoA: 81.2
Methylmalonyl-CoA: 80.7
inactive: 79.3
Ethylmalonyl-CoA: 52.6
Isobutyryl-CoA: 45.6
Propionyl-CoA: 43.3
CHC-CoA: 43.3
2-Methylbutyryl-CoA: 36.8
Acetyl-CoA: 35.3
Benzoyl-CoA: 31.9
trans-1,2-CPDA: 29.0
fatty_acid: 22.1
3-Methylbutyryl-CoA: 14.6

plm2-3: mal - mal

PKS_AT (563..834): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 185.7
inactive: 94.8
Methoxymalonyl-CoA: 82.2
Methylmalonyl-CoA: 77.9
Propionyl-CoA: 46.9
Ethylmalonyl-CoA: 42.4
CHC-CoA: 33.3
2-Methylbutyryl-CoA: 31.5
Isobutyryl-CoA: 29.7
trans-1,2-CPDA: 27.3
Benzoyl-CoA: 26.6
Acetyl-CoA: 26.0
fatty_acid: 17.4
3-Methylbutyryl-CoA: 6.1

PKS_AT (2308..2578): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 199.0
inactive: 90.4
Methylmalonyl-CoA: 77.7
Methoxymalonyl-CoA: 77.6
Ethylmalonyl-CoA: 41.9
CHC-CoA: 37.8
Propionyl-CoA: 37.2
Acetyl-CoA: 31.6
2-Methylbutyryl-CoA: 31.3
trans-1,2-CPDA: 30.4
Benzoyl-CoA: 30.2
Isobutyryl-CoA: 23.7
fatty_acid: 18.1
3-Methylbutyryl-CoA: 13.7

plmJK: X

AMP-binding (470..900): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TGYQVTALGK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor