BGC0000124 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000124 (Streptomyces piomogenus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	49,420	High	piericidin A1	PKS

BGC0000124 - Region 1 - T1PKS

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Location: 1 - 49,420 nt. (total: 49,420 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 49,421
Label		BGC0000124

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

AEZ54377.1: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

AEZ54384.1: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: methyltransferase

AEZ54383.1: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: hydrolase

AEZ54382.1: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Ligases: synthase

AEZ54374.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

AEZ54375.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AEZ54376.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

AEZ54378.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AEZ54379.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000124.5	1.00	PKS	piericidin A1	Streptomyces piomogenus
BGC0001742.5	0.77	PKS	piericidin A1	Candidatus Streptomyces philanthi bv. triangulum
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001169.5	0.70	PKS	piericidin A1	Streptomyces sp. SCSIO 03032
BGC0000018.5	0.68	PKS	angolamycin	Streptomyces eurythermus
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000124.5	1.00	PKS	piericidin A1	Streptomyces piomogenus
BGC0001742.5	0.77	PKS	piericidin A1	Candidatus Streptomyces philanthi bv. triangulum
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001169.5	0.70	PKS	piericidin A1	Streptomyces sp. SCSIO 03032
BGC0000018.5	0.68	PKS	angolamycin	Streptomyces eurythermus
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 49420:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ccmal) + (Me-ohmal - Me-ccmal) + (Me-redmal) + (ccmal) + (Me-mal - mal)
Non-colinear ordering:: AEZ54374.1 + AEZ54375.1 + AEZ54377.1 + AEZ54378.1 + AEZ54379.1

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AEZ54374.1: mmal - mmal

PKS_AT (320..624): mmal

ATSignature: Isobutyryl-CoA, 2-Methylbutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 75.0%; 2-Methylbutyryl-CoA: 75.0%; Methylmalonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 145.2
Ethylmalonyl-CoA: 140.4
Methoxymalonyl-CoA: 125.0
2-Methylbutyryl-CoA: 123.7
Propionyl-CoA: 108.5
trans-1,2-CPDA: 98.0
CHC-CoA: 89.5
Malonyl-CoA: 52.5
Isobutyryl-CoA: 52.2
3-Methylbutyryl-CoA: 36.4
Benzoyl-CoA: 32.5
Acetyl-CoA: 24.1
fatty_acid: 21.8
inactive: 0.0

PKS_AT (1310..1604): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 222.9
Ethylmalonyl-CoA: 221.4
Methoxymalonyl-CoA: 168.8
2-Methylbutyryl-CoA: 155.4
trans-1,2-CPDA: 148.8
Propionyl-CoA: 116.6
Malonyl-CoA: 109.0
CHC-CoA: 104.1
Isobutyryl-CoA: 84.1
Benzoyl-CoA: 65.3
Acetyl-CoA: 49.8
3-Methylbutyryl-CoA: 30.7
fatty_acid: 30.1
inactive: 21.6

AEZ54375.1: mmal - mmal

PKS_AT (561..853): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 222.8
Ethylmalonyl-CoA: 221.7
Methoxymalonyl-CoA: 173.3
2-Methylbutyryl-CoA: 155.7
trans-1,2-CPDA: 147.8
Propionyl-CoA: 116.8
CHC-CoA: 111.8
Malonyl-CoA: 107.3
Isobutyryl-CoA: 83.1
Benzoyl-CoA: 66.5
Acetyl-CoA: 54.5
fatty_acid: 36.1
3-Methylbutyryl-CoA: 31.8
inactive: 22.0

PKS_AT (2099..2391): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 221.1
Ethylmalonyl-CoA: 219.2
Methoxymalonyl-CoA: 171.9
2-Methylbutyryl-CoA: 155.2
trans-1,2-CPDA: 150.1
Propionyl-CoA: 120.6
Malonyl-CoA: 107.6
CHC-CoA: 106.2
Isobutyryl-CoA: 85.8
Benzoyl-CoA: 67.9
Acetyl-CoA: 51.6
3-Methylbutyryl-CoA: 33.7
fatty_acid: 31.4
inactive: 18.8

AEZ54377.1: mmal

PKS_AT (539..833): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 220.1
Ethylmalonyl-CoA: 215.6
Methoxymalonyl-CoA: 169.9
2-Methylbutyryl-CoA: 151.9
trans-1,2-CPDA: 148.0
Propionyl-CoA: 120.3
CHC-CoA: 102.6
Malonyl-CoA: 102.1
Isobutyryl-CoA: 86.1
Benzoyl-CoA: 66.5
Acetyl-CoA: 49.4
3-Methylbutyryl-CoA: 32.2
fatty_acid: 29.4
inactive: 18.4

AEZ54378.1: mal

PKS_AT (573..872): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 223.5
inactive: 116.1
Methoxymalonyl-CoA: 58.6
Methylmalonyl-CoA: 56.7
Propionyl-CoA: 39.8
Isobutyryl-CoA: 37.4
Benzoyl-CoA: 27.4
2-Methylbutyryl-CoA: 23.8
Ethylmalonyl-CoA: 18.9
fatty_acid: 17.1
Acetyl-CoA: 16.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
CHC-CoA: -0.2

AEZ54379.1: mmal - mal

PKS_AT (618..910): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 223.5
Ethylmalonyl-CoA: 222.1
Methoxymalonyl-CoA: 173.8
2-Methylbutyryl-CoA: 157.4
trans-1,2-CPDA: 146.9
Propionyl-CoA: 117.9
CHC-CoA: 109.2
Malonyl-CoA: 107.1
Isobutyryl-CoA: 83.4
Benzoyl-CoA: 67.0
Acetyl-CoA: 54.2
fatty_acid: 36.1
3-Methylbutyryl-CoA: 31.5
inactive: 22.0

PKS_AT (1650..1953): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 179.3
inactive: 79.2
Methoxymalonyl-CoA: 42.9
Methylmalonyl-CoA: 40.0
Isobutyryl-CoA: 21.4
Propionyl-CoA: 20.6
Acetyl-CoA: 13.7
Ethylmalonyl-CoA: 12.0
fatty_acid: 11.1
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor