BGC0000125 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000125 (Streptomyces natalensis)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS-like	1	84,985	pimaricin	PKS	100%

BGC0000125 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 84,985 nt. (total: 84,985 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 84,986
Label		BGC0000125

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000125	1.35	Polyketide	pimaricin	Streptomyces natalensis
BGC0001690	1.11	Polyketide	natamycin	Streptomyces gilvosporeus
BGC0000108	0.83	Polyketide	natamycin	Streptomyces chattanoogensis
BGC0000016	0.83	Other	amphotericin B	Streptomyces nodosus
BGC0002099	0.64	RiPP	biarylite-YFH	Planomonospora sp. ID107089
BGC0002084	0.64	RiPP	biarylitide YYH, biarylitide YFH	Planomonospora sp. ID82291
BGC0002631	0.61	RiPP	myxarylin	Pyxidicoccus fallax
BGC0000138	0.59	Polyketide	rimocidin	Streptomyces diastaticus
BGC0001817	0.57	Other	vancosamine	Streptomyces sp. WAC2288
BGC0001544	0.50	NRP, Polyketide	chlorflavonin	Aspergillus campestris IBT 28561

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000125	1.00	Polyketide	pimaricin	Streptomyces natalensis
BGC0001690	0.94	Polyketide	natamycin	Streptomyces gilvosporeus
BGC0000108	0.82	Polyketide	natamycin	Streptomyces chattanoogensis
BGC0000016	0.76	Other	amphotericin B	Streptomyces nodosus
BGC0002099	0.65	RiPP	biarylite-YFH	Planomonospora sp. ID107089
BGC0002084	0.65	RiPP	biarylitide YYH, biarylitide YFH	Planomonospora sp. ID82291
BGC0001163	0.65	Polyketide	1-heptadecene	Moorea producens 3L
BGC0001164	0.65	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002031	0.65	Alkaloid	kitasetaline	Kitasatospora setae KM-6054
BGC0000160	0.65	Polyketide	terreic acid	Aspergillus terreus NIH2624

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 84985:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal - ccmal - ccmal - ccmal) + (X - mal) + (ccmal - ohmal - Me-ohmal - ohmal - ohmal - ohmal) + (ccmal) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

pimS4

PKS_AT (550..835): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 229.7
inactive: 105.0
Methoxymalonyl-CoA: 59.9
Methylmalonyl-CoA: 56.0
Propionyl-CoA: 28.2
Acetyl-CoA: 25.0
Isobutyryl-CoA: 23.9
Benzoyl-CoA: 20.8
Ethylmalonyl-CoA: 15.4
CHC-CoA: 8.5
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

pimS3

PKS_AT (551..836): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 229.0
inactive: 104.2
Methoxymalonyl-CoA: 62.2
Methylmalonyl-CoA: 54.3
Propionyl-CoA: 31.4
Isobutyryl-CoA: 26.7
Acetyl-CoA: 23.6
Ethylmalonyl-CoA: 17.9
Benzoyl-CoA: 17.3
2-Methylbutyryl-CoA: 13.9
fatty_acid: 7.0
CHC-CoA: 5.4
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

pimS2

PKS_AT (561..851): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.7
inactive: 88.5
Methoxymalonyl-CoA: 56.7
Methylmalonyl-CoA: 47.2
Acetyl-CoA: 27.7
Propionyl-CoA: 27.3
Benzoyl-CoA: 23.6
Ethylmalonyl-CoA: 19.2
Isobutyryl-CoA: 18.8
2-Methylbutyryl-CoA: 18.7
CHC-CoA: 16.6
trans-1,2-CPDA: 10.1
fatty_acid: 9.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (2317..2616): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 199.6
inactive: 85.8
Methoxymalonyl-CoA: 70.3
Methylmalonyl-CoA: 60.0
Isobutyryl-CoA: 33.6
Propionyl-CoA: 29.9
Ethylmalonyl-CoA: 22.6
CHC-CoA: 22.1
Acetyl-CoA: 21.2
Benzoyl-CoA: 19.3
fatty_acid: 13.1
2-Methylbutyryl-CoA: 11.2
trans-1,2-CPDA: 9.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (3860..4157): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 209.6
Ethylmalonyl-CoA: 193.4
Methoxymalonyl-CoA: 165.6
2-Methylbutyryl-CoA: 128.4
trans-1,2-CPDA: 126.0
Propionyl-CoA: 105.8
CHC-CoA: 94.8
Malonyl-CoA: 91.5
Isobutyryl-CoA: 73.1
Benzoyl-CoA: 62.1
Acetyl-CoA: 34.7
fatty_acid: 28.8
3-Methylbutyryl-CoA: 26.6
inactive: 17.4

PKS_AT (5393..5693): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.8
inactive: 98.9
Methoxymalonyl-CoA: 72.9
Methylmalonyl-CoA: 61.0
Isobutyryl-CoA: 44.3
Propionyl-CoA: 30.0
Benzoyl-CoA: 19.9
Ethylmalonyl-CoA: 17.4
2-Methylbutyryl-CoA: 17.3
Acetyl-CoA: 15.8
fatty_acid: 13.5
3-Methylbutyryl-CoA: 10.5
CHC-CoA: 8.8
trans-1,2-CPDA: 0.0

PKS_AT (6913..7198): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 223.8
inactive: 100.2
Methoxymalonyl-CoA: 56.7
Methylmalonyl-CoA: 50.6
Isobutyryl-CoA: 27.9
Propionyl-CoA: 23.0
Acetyl-CoA: 22.7
Benzoyl-CoA: 21.3
Ethylmalonyl-CoA: 14.6
fatty_acid: 8.3
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (8430..8723): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 226.6
inactive: 106.3
Methoxymalonyl-CoA: 62.1
Methylmalonyl-CoA: 60.0
Isobutyryl-CoA: 38.2
Propionyl-CoA: 29.9
Benzoyl-CoA: 23.1
Ethylmalonyl-CoA: 22.5
Acetyl-CoA: 20.6
2-Methylbutyryl-CoA: 14.6
CHC-CoA: 11.2
fatty_acid: 10.7
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

pimS0

AMP-binding (29..446): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LFQFRAVTGK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

PKS_AT (1209..1511): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 182.5
inactive: 72.9
Methoxymalonyl-CoA: 70.4
Methylmalonyl-CoA: 60.7
Isobutyryl-CoA: 35.1
Ethylmalonyl-CoA: 33.6
Propionyl-CoA: 32.9
Acetyl-CoA: 29.9
fatty_acid: 25.2
Benzoyl-CoA: 24.0
CHC-CoA: 23.1
2-Methylbutyryl-CoA: 15.3
trans-1,2-CPDA: 15.1
3-Methylbutyryl-CoA: 0.0

pimS1

PKS_AT (565..864): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.1
inactive: 101.9
Methoxymalonyl-CoA: 63.8
Methylmalonyl-CoA: 54.0
Isobutyryl-CoA: 46.0
Propionyl-CoA: 31.7
Benzoyl-CoA: 22.7
Acetyl-CoA: 19.9
Ethylmalonyl-CoA: 19.3
2-Methylbutyryl-CoA: 14.8
trans-1,2-CPDA: 12.6
CHC-CoA: 7.3
3-Methylbutyryl-CoA: 6.9
fatty_acid: 0.0

PKS_AT (2103..2393): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.5
inactive: 101.6
Methoxymalonyl-CoA: 51.1
Methylmalonyl-CoA: 42.0
Acetyl-CoA: 19.8
Propionyl-CoA: 18.9
Benzoyl-CoA: 15.5
2-Methylbutyryl-CoA: 11.5
fatty_acid: 7.7
CHC-CoA: 5.1
Isobutyryl-CoA: 4.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -3.9

PKS_AT (3829..4119): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 228.7
inactive: 108.0
Methoxymalonyl-CoA: 51.3
Methylmalonyl-CoA: 38.8
Propionyl-CoA: 28.9
Acetyl-CoA: 22.0
Benzoyl-CoA: 16.2
Ethylmalonyl-CoA: 12.9
fatty_acid: 11.7
Isobutyryl-CoA: 11.0
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.6

PKS_AT (5543..5833): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.8
inactive: 108.1
Methoxymalonyl-CoA: 63.1
Methylmalonyl-CoA: 47.4
Propionyl-CoA: 27.6
Acetyl-CoA: 24.7
Ethylmalonyl-CoA: 21.0
2-Methylbutyryl-CoA: 18.1
Benzoyl-CoA: 17.6
fatty_acid: 17.4
Isobutyryl-CoA: 13.1
CHC-CoA: 10.4
trans-1,2-CPDA: 6.9
3-Methylbutyryl-CoA: 0.0