BGC0000126 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000126 (Streptomyces platensis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	74,342	High	pladienolide B	PKS

BGC0000126 - Region 1 - T1PKS

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Location: 1 - 74,342 nt. (total: 74,342 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 74,343
Label		BGC0000126

Tailoring functions

Oxidoreductases: oxygenase

pldB: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

pldD: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

Ligases: ligase synthase

pldAI: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

pldAII: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

pldAIII: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

pldAIV: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

pldC: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000126.5	1.00	PKS	pladienolide B	Streptomyces platensis
BGC0002102.2	0.89	PKS	pladienolide B	Streptomyces platensis
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0000067.5	0.66	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000041.3	0.65	PKS	oronofacic acid	Pseudomonas syringae
BGC0000094.5	0.65	PKS, saccharide	methymycin	Streptomyces venezuelae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000126.5	1.00	PKS	pladienolide B	Streptomyces platensis
BGC0002102.2	0.89	PKS	pladienolide B	Streptomyces platensis
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0000067.5	0.66	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000041.3	0.65	PKS	oronofacic acid	Pseudomonas syringae
BGC0000094.5	0.65	PKS, saccharide	methymycin	Streptomyces venezuelae

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 74342:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ohmal - ccmal - Me-redmal) + (ccmal - Me-ccmal - Me-ohmal - ccmal) + (Me-ohmal - redmal) + (ohmal)
Non-colinear ordering:: pldAI + pldAII + pldAIII + pldAIV

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

pldAI: mmal - mmal - mal - mmal

PKS_AT (540..838): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.8
Ethylmalonyl-CoA: 204.2
Methoxymalonyl-CoA: 185.1
2-Methylbutyryl-CoA: 145.8
trans-1,2-CPDA: 130.1
CHC-CoA: 116.3
Propionyl-CoA: 112.2
Malonyl-CoA: 109.6
Benzoyl-CoA: 71.7
Isobutyryl-CoA: 68.6
Acetyl-CoA: 43.7
3-Methylbutyryl-CoA: 38.6
inactive: 36.4
fatty_acid: 31.3

PKS_AT (1556..1854): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 202.6
Ethylmalonyl-CoA: 187.5
Methoxymalonyl-CoA: 171.3
2-Methylbutyryl-CoA: 144.3
trans-1,2-CPDA: 110.5
CHC-CoA: 108.5
Malonyl-CoA: 101.1
Propionyl-CoA: 95.8
Isobutyryl-CoA: 67.8
Benzoyl-CoA: 58.7
Acetyl-CoA: 45.9
inactive: 36.3
fatty_acid: 36.3
3-Methylbutyryl-CoA: 35.8

PKS_AT (3122..3422): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 220.3
inactive: 122.0
Methoxymalonyl-CoA: 71.3
Methylmalonyl-CoA: 66.7
Isobutyryl-CoA: 40.9
Benzoyl-CoA: 32.9
Propionyl-CoA: 31.7
Acetyl-CoA: 30.3
Ethylmalonyl-CoA: 27.1
trans-1,2-CPDA: 19.7
2-Methylbutyryl-CoA: 14.2
CHC-CoA: 11.6
fatty_acid: 11.4
3-Methylbutyryl-CoA: 0.0

PKS_AT (4907..5205): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.3
Ethylmalonyl-CoA: 195.9
Methoxymalonyl-CoA: 181.8
2-Methylbutyryl-CoA: 146.9
trans-1,2-CPDA: 124.0
CHC-CoA: 114.1
Propionyl-CoA: 112.6
Malonyl-CoA: 104.6
Isobutyryl-CoA: 68.1
Benzoyl-CoA: 63.6
Acetyl-CoA: 46.3
3-Methylbutyryl-CoA: 45.6
inactive: 30.8
fatty_acid: 27.4

pldAII: mal - mmal - mmal - mal

PKS_AT (590..892): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.4
inactive: 107.0
Methylmalonyl-CoA: 63.7
Methoxymalonyl-CoA: 62.7
Isobutyryl-CoA: 45.3
Ethylmalonyl-CoA: 34.5
Propionyl-CoA: 30.2
Benzoyl-CoA: 27.7
2-Methylbutyryl-CoA: 23.7
Acetyl-CoA: 18.3
CHC-CoA: 16.4
fatty_acid: 8.6
trans-1,2-CPDA: 7.1
3-Methylbutyryl-CoA: 6.5

PKS_AT (2384..2681): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 209.1
Ethylmalonyl-CoA: 193.1
Methoxymalonyl-CoA: 181.2
2-Methylbutyryl-CoA: 142.6
trans-1,2-CPDA: 124.3
CHC-CoA: 111.3
Propionyl-CoA: 110.8
Malonyl-CoA: 108.8
Isobutyryl-CoA: 67.5
Benzoyl-CoA: 63.7
Acetyl-CoA: 48.0
3-Methylbutyryl-CoA: 45.8
inactive: 32.7
fatty_acid: 27.4

PKS_AT (4204..4502): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.3
Ethylmalonyl-CoA: 195.9
Methoxymalonyl-CoA: 181.8
2-Methylbutyryl-CoA: 146.9
trans-1,2-CPDA: 124.0
CHC-CoA: 114.1
Propionyl-CoA: 112.6
Malonyl-CoA: 104.6
Isobutyryl-CoA: 68.1
Benzoyl-CoA: 63.6
Acetyl-CoA: 46.3
3-Methylbutyryl-CoA: 45.6
inactive: 30.8
fatty_acid: 27.4

PKS_AT (5757..6056): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 200.4
inactive: 92.1
Methoxymalonyl-CoA: 61.8
Methylmalonyl-CoA: 60.5
Isobutyryl-CoA: 43.1
Ethylmalonyl-CoA: 34.3
Propionyl-CoA: 30.8
Benzoyl-CoA: 29.3
2-Methylbutyryl-CoA: 27.9
CHC-CoA: 19.2
fatty_acid: 14.8
Acetyl-CoA: 14.7
trans-1,2-CPDA: 7.7
3-Methylbutyryl-CoA: 4.3

pldAIII: mmal - mal

PKS_AT (569..864): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 218.5
Ethylmalonyl-CoA: 210.2
Methoxymalonyl-CoA: 183.3
2-Methylbutyryl-CoA: 168.2
trans-1,2-CPDA: 126.3
Malonyl-CoA: 120.5
Propionyl-CoA: 110.2
CHC-CoA: 110.0
Isobutyryl-CoA: 90.9
Benzoyl-CoA: 69.3
Acetyl-CoA: 48.9
3-Methylbutyryl-CoA: 34.9
fatty_acid: 30.3
inactive: 22.4

PKS_AT (2124..2424): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 222.1
inactive: 95.8
Methoxymalonyl-CoA: 65.5
Methylmalonyl-CoA: 55.9
Propionyl-CoA: 36.7
Ethylmalonyl-CoA: 33.5
2-Methylbutyryl-CoA: 28.9
Acetyl-CoA: 24.2
Isobutyryl-CoA: 23.9
Benzoyl-CoA: 17.9
CHC-CoA: 17.6
trans-1,2-CPDA: 11.6
fatty_acid: 11.2
3-Methylbutyryl-CoA: 0.0

pldAIV: mal

PKS_AT (591..893): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.8
inactive: 94.9
Methoxymalonyl-CoA: 67.9
Methylmalonyl-CoA: 65.4
Isobutyryl-CoA: 35.8
Propionyl-CoA: 35.4
Benzoyl-CoA: 26.2
Ethylmalonyl-CoA: 22.1
2-Methylbutyryl-CoA: 14.8
fatty_acid: 13.1
Acetyl-CoA: 11.8
trans-1,2-CPDA: 9.4
CHC-CoA: 7.8
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor