BGC0000127 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000127 (Pseudomonas protegens Pf-5)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	33,648	High	pyoluteorin	PKS

BGC0000127 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 33,648 nt. (total: 33,648 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 33,649
Label		BGC0000127

Tailoring functions

Oxidoreductases: dehydrogenase

AAX56379.1: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

pltE: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: acylase halogenase

pltA: flavin-dependent pyrrole halogenase

halogenases: flavin-dependent pyrrole halogenase
smcogs: SMCOG1119: halogenase

pltB: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

pltD: halogenase

smcogs: SMCOG1119: halogenase

pltM: conventional flavin-dependent halogenase

halogenases: conventional flavin-dependent halogenase
smcogs: SMCOG1119: halogenase

Hydrolases: esterase

pltG: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

pltC: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

pltF: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000127.5	0.99	PKS	pyoluteorin	Pseudomonas protegens Pf-5
BGC0000128.3	0.76	PKS	pyoluteorin	Pseudomonas sp. M18
BGC0002341.2	0.75	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.67	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.67	PKS	oronofacic acid	Pseudomonas syringae
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000127.5	0.99	PKS	pyoluteorin	Pseudomonas protegens Pf-5
BGC0000128.3	0.76	PKS	pyoluteorin	Pseudomonas sp. M18
BGC0002341.2	0.75	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.67	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.67	PKS	oronofacic acid	Pseudomonas syringae
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 33648:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (ohmal)
Non-colinear ordering:: pltB + pltC

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

pltB: mal

PKS_AT (536..829): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; Methylmalonyl-CoA: 70.8%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 120.0
Methylmalonyl-CoA: 113.6
Methoxymalonyl-CoA: 93.0
Isobutyryl-CoA: 79.0
2-Methylbutyryl-CoA: 67.0
Propionyl-CoA: 62.9
Ethylmalonyl-CoA: 60.7
trans-1,2-CPDA: 46.5
fatty_acid: 45.4
CHC-CoA: 42.7
Benzoyl-CoA: 27.5
Acetyl-CoA: 25.4
inactive: 21.8
3-Methylbutyryl-CoA: 20.6

pltC: mal

PKS_AT (569..869): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 186.4
inactive: 109.7
Methylmalonyl-CoA: 71.9
Methoxymalonyl-CoA: 66.8
Propionyl-CoA: 53.0
2-Methylbutyryl-CoA: 41.9
Ethylmalonyl-CoA: 40.2
CHC-CoA: 30.8
Isobutyryl-CoA: 28.2
trans-1,2-CPDA: 27.0
Benzoyl-CoA: 23.7
fatty_acid: 23.3
Acetyl-CoA: 19.9
3-Methylbutyryl-CoA: 0.0

pltF: Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (10..404): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLLYLALVCK
Nearest Stachelhaus code(s):: Pro DLLYLALVCK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor