BGC0000128 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000128 (Pseudomonas sp. M18)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	32,800	pyoluteorin	PKS	100%

BGC0000128 - Region 1 - T1PKS

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Location: 1 - 32,800 nt. (total: 32,800 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 32,801
Label		BGC0000128

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000128	1.00	Polyketide	pyoluteorin	Pseudomonas sp. M18
BGC0000127	0.44	Polyketide	pyoluteorin	Pseudomonas protegens Pf-5
BGC0001160	0.34	Polyketide	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000016	0.33	Other	amphotericin B	Streptomyces nodosus
BGC0002341	0.32	Other	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0001137	0.31	Alkaloid	marinacarboline A, marinacarboline B, marinacarboline C, marinacarboline D	Marinactinospora thermotolerans
BGC0001463	0.31	Other	2,3,4,5-tetrabromopyrrole	Pseudoalteromonas piscicida
BGC0001464	0.31	Other	tetrabromopyrrole	Pseudoalteromonas sp. PS5
BGC0002476	0.31	NRP	enterobactin	Escherichia coli str. K-12 substr. MG1655
BGC0002597	0.30	NRP, Polyketide	nocuolactylates	Nodularia sp. LEGE 06071

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000128	1.00	Polyketide	pyoluteorin	Pseudomonas sp. M18
BGC0000127	0.74	Polyketide	pyoluteorin	Pseudomonas protegens Pf-5
BGC0001160	0.67	Polyketide	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000016	0.66	Other	amphotericin B	Streptomyces nodosus
BGC0002341	0.65	Other	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0001137	0.65	Alkaloid	marinacarboline A, marinacarboline B, marinacarboline C, marinacarboline D	Marinactinospora thermotolerans
BGC0001463	0.65	Other	2,3,4,5-tetrabromopyrrole	Pseudoalteromonas piscicida
BGC0001464	0.64	Other	tetrabromopyrrole	Pseudoalteromonas sp. PS5
BGC0002476	0.64	NRP	enterobactin	Escherichia coli str. K-12 substr. MG1655
BGC0002597	0.64	NRP, Polyketide	nocuolactylates	Nodularia sp. LEGE 06071

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 32800:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (ohmal)
Non-colinear ordering:: pltB + pltC

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

pltB

PKS_AT (534..826): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; 2-Methylbutyryl-CoA: 66.7%; Methylmalonyl-CoA: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 121.7
Methylmalonyl-CoA: 104.7
Methoxymalonyl-CoA: 87.9
Isobutyryl-CoA: 71.3
2-Methylbutyryl-CoA: 66.7
Ethylmalonyl-CoA: 63.1
Propionyl-CoA: 55.0
CHC-CoA: 46.0
fatty_acid: 44.4
trans-1,2-CPDA: 39.0
inactive: 36.2
Benzoyl-CoA: 30.9
Acetyl-CoA: 25.9
3-Methylbutyryl-CoA: 14.5

pltC

PKS_AT (570..868): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 176.6
inactive: 100.1
Methylmalonyl-CoA: 65.1
Methoxymalonyl-CoA: 63.7
Propionyl-CoA: 45.6
2-Methylbutyryl-CoA: 44.4
Isobutyryl-CoA: 32.6
CHC-CoA: 29.4
trans-1,2-CPDA: 24.5
Ethylmalonyl-CoA: 23.9
Benzoyl-CoA: 19.2
fatty_acid: 18.3
Acetyl-CoA: 16.3
3-Methylbutyryl-CoA: 15.5

pltF

Search NORINE for peptide: strict or relaxed

AMP-binding (9..403): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLLYLALVCK
Nearest Stachelhaus code(s):: Pro DLLYLALVCK (88% 8Å match)
Stachelhaus code match:: 100% (strong)