BGC0000133 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000133 (Amycolatopsis orientalis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	82,338	High	quartromicin A1	PKS

BGC0000133 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 82,338 nt. (total: 82,338 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 82,339
Label		BGC0000133

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

AFI56999.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

AFI57027.1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: aminotransferase transferase

AFI57002.1: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

AFI57009.1: aminotransferase class V

smcogs: SMCOG1139: aminotransferase class V

Hydrolases: esterase hydrolase phosphatase

AFI57003.1: thioesterase

smcogs: SMCOG1004: thioesterase

AFI57008.1: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

AFI57024.1: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

Lyases: cyclase

AFI57012.1: Diels-Alderase cyclase

extras: DA_cyclase: Diels-Alderase cyclase

Ligases: synthase

AFI57005.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AFI57006.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

AFI57007.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

AFI57023.1: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000133.5	0.96	PKS	quartromicin A1	Amycolatopsis orientalis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0000041.3	0.66	PKS	oronofacic acid	Pseudomonas syringae
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001164.3	0.66	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.66	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002192.3	0.64	PKS	FR901512	fungal sp. No.14919

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000133.5	1.00	PKS	quartromicin A1	Amycolatopsis orientalis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0000041.3	0.66	PKS	oronofacic acid	Pseudomonas syringae
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001164.3	0.66	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.66	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002192.3	0.64	PKS	FR901512	fungal sp. No.14919

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 3934 - 70896:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-ccmal - Me-ccmal - ccmal) + (Me-ccmal) + (mal)
Non-colinear ordering:: AFI57005.1 + AFI57006.1 + AFI57007.1

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AFI57005.1: mal - mmal - mmal - mal

PKS_AT (493..778): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 205.9
inactive: 116.8
Methoxymalonyl-CoA: 69.0
Methylmalonyl-CoA: 62.7
Propionyl-CoA: 44.9
Isobutyryl-CoA: 34.7
Ethylmalonyl-CoA: 22.8
Acetyl-CoA: 16.9
2-Methylbutyryl-CoA: 15.2
fatty_acid: 12.7
Benzoyl-CoA: 10.5
CHC-CoA: 10.1
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (1471..1758): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 217.4
Ethylmalonyl-CoA: 211.8
Methoxymalonyl-CoA: 175.7
trans-1,2-CPDA: 145.8
2-Methylbutyryl-CoA: 141.1
Propionyl-CoA: 114.1
Malonyl-CoA: 103.1
CHC-CoA: 85.7
Isobutyryl-CoA: 81.4
Benzoyl-CoA: 65.2
Acetyl-CoA: 48.3
fatty_acid: 42.4
3-Methylbutyryl-CoA: 36.5
inactive: 22.9

PKS_AT (3116..3408): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 218.4
Ethylmalonyl-CoA: 206.1
Methoxymalonyl-CoA: 166.7
2-Methylbutyryl-CoA: 149.3
trans-1,2-CPDA: 127.1
Propionyl-CoA: 106.6
Malonyl-CoA: 90.8
CHC-CoA: 90.0
Benzoyl-CoA: 73.8
Isobutyryl-CoA: 67.8
fatty_acid: 33.6
Acetyl-CoA: 32.4
3-Methylbutyryl-CoA: 29.7
inactive: 14.2

PKS_AT (4787..5068): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 217.4
inactive: 108.2
Methoxymalonyl-CoA: 65.7
Methylmalonyl-CoA: 55.2
Propionyl-CoA: 43.6
Benzoyl-CoA: 31.4
Ethylmalonyl-CoA: 29.6
Isobutyryl-CoA: 28.2
2-Methylbutyryl-CoA: 23.4
Acetyl-CoA: 22.6
fatty_acid: 17.4
trans-1,2-CPDA: 16.6
CHC-CoA: 15.1
3-Methylbutyryl-CoA: 0.0

AFI57006.1: mmal

PKS_AT (568..867): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 213.6
Ethylmalonyl-CoA: 209.4
Methoxymalonyl-CoA: 176.2
2-Methylbutyryl-CoA: 155.5
trans-1,2-CPDA: 132.8
Propionyl-CoA: 116.3
Malonyl-CoA: 112.9
CHC-CoA: 106.5
Isobutyryl-CoA: 76.9
Benzoyl-CoA: 56.5
Acetyl-CoA: 38.9
3-Methylbutyryl-CoA: 36.7
fatty_acid: 29.9
inactive: 16.4

AFI57007.1: mal

PKS_AT (563..854): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 204.2
inactive: 105.5
Methoxymalonyl-CoA: 62.9
Methylmalonyl-CoA: 48.8
Isobutyryl-CoA: 35.1
Propionyl-CoA: 30.7
Acetyl-CoA: 14.9
Benzoyl-CoA: 11.9
fatty_acid: 10.6
CHC-CoA: 6.8
trans-1,2-CPDA: 6.3
Ethylmalonyl-CoA: 5.2
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor