BGC0000144 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000144 (Streptomyces albus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,butyrolactone	1	127,961	High	salinomycin	PKS:Type I

BGC0000144 - Region 1 - T1PKS,butyrolactone

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Location: 1 - 127,961 nt. (total: 127,961 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
butyrolactone: AfsA

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 127,962
Label		BGC0000144

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase reductase

AEZ53933.1: alkyl hydroperoxide reductase/ Thiol specific

smcogs: SMCOG1196: alkyl hydroperoxide reductase/ Thiol specific

AEZ53938.1: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

AEZ53943.1: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

AEZ53970.1: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

slnC: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

slnF: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: PPTase acylase aminotransferase

AEZ53937.1: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

AEZ53972.1: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

slnA4: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

slnA6: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

slnA7: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase

slnDI: thioesterase

smcogs: SMCOG1004: thioesterase

slnDII: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

AEZ53944.1: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

AEZ53969.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

slnA1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

slnA2: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

slnA3: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

slnA5: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

slnA8: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

slnA9: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Translocases: transporter

AEZ53979.1: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000144.5	1.01	PKS	salinomycin	Streptomyces albus
BGC0001160.5	0.55	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.55	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001273.3	0.47	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.47	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.46	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.46	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.42	PKS	oronofacic acid	Pseudomonas syringae
BGC0000018.5	0.39	PKS	angolamycin	Streptomyces eurythermus
BGC0000067.5	0.34	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000144.5	1.00	PKS	salinomycin	Streptomyces albus
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.73	PKS	oronofacic acid	Pseudomonas syringae
BGC0000018.5	0.71	PKS	angolamycin	Streptomyces eurythermus
BGC0000067.5	0.68	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 122658:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - pk - redmal) + (Me-ccmal - redmal) + (mal - ohmal) + (Me-ohmal) + (Me-redmal - pk) + (Me-mal) + (pk) + (Me-ohmal - redmal) + (pk)
Non-colinear ordering:: slnA1 + slnA2 + slnA3 + slnA4 + slnA5 + slnA6 + slnA7 + slnA8 + slnA9

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 112290:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - pk - redmal) + (Me-ccmal - redmal) + (mal - ohmal) + (Me-ohmal) + (Me-redmal - pk) + (Me-mal) + (pk) + (Me-ohmal - redmal) + (pk)
Non-colinear ordering:: slnA1 + slnA2 + slnA3 + slnA4 + slnA5 + slnA6 + slnA7 + slnA8 + slnA9

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

slnA1: mal - pk - mal

PKS_AT (545..834): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 224.6
inactive: 100.7
Methoxymalonyl-CoA: 68.2
Methylmalonyl-CoA: 57.9
Isobutyryl-CoA: 41.8
Propionyl-CoA: 35.0
Ethylmalonyl-CoA: 34.6
2-Methylbutyryl-CoA: 30.0
Benzoyl-CoA: 25.0
Acetyl-CoA: 24.3
CHC-CoA: 16.6
trans-1,2-CPDA: 14.4
fatty_acid: 11.8
3-Methylbutyryl-CoA: 0.0

PKS_AT (1554..1854): pk

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Methylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 182.2
Ethylmalonyl-CoA: 173.7
Methoxymalonyl-CoA: 147.1
2-Methylbutyryl-CoA: 125.1
trans-1,2-CPDA: 93.0
Propionyl-CoA: 89.9
CHC-CoA: 89.4
Malonyl-CoA: 71.6
Isobutyryl-CoA: 68.4
3-Methylbutyryl-CoA: 38.0
fatty_acid: 34.9
Benzoyl-CoA: 33.3
Acetyl-CoA: 32.6
inactive: 14.9

PKS_AT (3311..3598): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 220.2
inactive: 112.3
Methoxymalonyl-CoA: 68.2
Methylmalonyl-CoA: 66.1
Isobutyryl-CoA: 32.3
Ethylmalonyl-CoA: 29.1
Propionyl-CoA: 25.0
Benzoyl-CoA: 21.1
Acetyl-CoA: 20.6
2-Methylbutyryl-CoA: 16.0
fatty_acid: 14.7
CHC-CoA: 14.7
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

slnA2: mmal - mal

PKS_AT (571..867): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 216.2
Ethylmalonyl-CoA: 215.8
Methoxymalonyl-CoA: 182.0
2-Methylbutyryl-CoA: 152.1
trans-1,2-CPDA: 131.1
Propionyl-CoA: 111.9
Malonyl-CoA: 97.0
CHC-CoA: 94.6
Isobutyryl-CoA: 70.3
3-Methylbutyryl-CoA: 57.6
Benzoyl-CoA: 48.7
Acetyl-CoA: 47.0
inactive: 34.9
fatty_acid: 33.9

PKS_AT (2307..2595): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 215.1
inactive: 108.3
Methoxymalonyl-CoA: 69.1
Methylmalonyl-CoA: 61.8
Isobutyryl-CoA: 28.1
Propionyl-CoA: 26.8
Ethylmalonyl-CoA: 25.6
Acetyl-CoA: 19.8
Benzoyl-CoA: 18.5
CHC-CoA: 11.4
fatty_acid: 9.5
2-Methylbutyryl-CoA: 5.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

slnA3: mal - mal

PKS_AT (573..861): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.2
inactive: 112.9
Methoxymalonyl-CoA: 69.0
Methylmalonyl-CoA: 60.6
Ethylmalonyl-CoA: 27.5
Propionyl-CoA: 24.6
Isobutyryl-CoA: 24.2
Acetyl-CoA: 18.7
Benzoyl-CoA: 17.7
CHC-CoA: 14.2
fatty_acid: 8.9
2-Methylbutyryl-CoA: 6.1
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (1627..1915): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.4
inactive: 112.4
Methoxymalonyl-CoA: 71.1
Methylmalonyl-CoA: 64.0
Isobutyryl-CoA: 33.0
Propionyl-CoA: 28.6
Ethylmalonyl-CoA: 25.6
Acetyl-CoA: 19.4
Benzoyl-CoA: 18.7
CHC-CoA: 11.4
fatty_acid: 9.5
2-Methylbutyryl-CoA: 4.8
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

slnA4: mmal

PKS_AT (576..872): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 228.5
Ethylmalonyl-CoA: 224.6
Methoxymalonyl-CoA: 190.4
2-Methylbutyryl-CoA: 161.8
trans-1,2-CPDA: 146.2
Propionyl-CoA: 121.4
CHC-CoA: 108.5
Malonyl-CoA: 107.2
Isobutyryl-CoA: 79.0
Benzoyl-CoA: 63.3
fatty_acid: 47.9
3-Methylbutyryl-CoA: 47.0
Acetyl-CoA: 38.4
inactive: 31.1

slnA5: mmal - pk

PKS_AT (588..884): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 224.3
Ethylmalonyl-CoA: 219.0
Methoxymalonyl-CoA: 181.9
2-Methylbutyryl-CoA: 154.1
trans-1,2-CPDA: 140.9
Propionyl-CoA: 115.4
CHC-CoA: 102.1
Malonyl-CoA: 102.0
Isobutyryl-CoA: 78.6
Benzoyl-CoA: 63.4
3-Methylbutyryl-CoA: 47.4
Acetyl-CoA: 40.5
inactive: 36.0
fatty_acid: 24.9

PKS_AT (2683..2983): pk

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Methylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 183.0
Ethylmalonyl-CoA: 173.4
Methoxymalonyl-CoA: 147.5
2-Methylbutyryl-CoA: 129.8
trans-1,2-CPDA: 95.7
CHC-CoA: 92.2
Propionyl-CoA: 90.1
Isobutyryl-CoA: 72.7
Malonyl-CoA: 70.1
3-Methylbutyryl-CoA: 40.8
fatty_acid: 38.6
Acetyl-CoA: 38.5
Benzoyl-CoA: 36.0
inactive: 14.5

slnA6: mmal

PKS_AT (586..882): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 223.7
Ethylmalonyl-CoA: 219.2
Methoxymalonyl-CoA: 185.6
2-Methylbutyryl-CoA: 165.4
trans-1,2-CPDA: 144.0
Propionyl-CoA: 122.9
CHC-CoA: 114.4
Malonyl-CoA: 101.8
Isobutyryl-CoA: 81.2
Benzoyl-CoA: 59.8
fatty_acid: 43.7
Acetyl-CoA: 40.8
3-Methylbutyryl-CoA: 39.0
inactive: 35.6

slnA7: pk

PKS_AT (563..859): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 222.3
Methylmalonyl-CoA: 221.4
Methoxymalonyl-CoA: 169.4
2-Methylbutyryl-CoA: 154.1
trans-1,2-CPDA: 145.6
CHC-CoA: 109.1
Propionyl-CoA: 107.9
Malonyl-CoA: 103.0
Isobutyryl-CoA: 73.6
Benzoyl-CoA: 63.5
inactive: 43.0
Acetyl-CoA: 42.5
3-Methylbutyryl-CoA: 38.8
fatty_acid: 30.8

slnA8: mmal - mal

PKS_AT (572..868): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 223.7
Ethylmalonyl-CoA: 221.0
Methoxymalonyl-CoA: 176.8
2-Methylbutyryl-CoA: 153.0
trans-1,2-CPDA: 148.3
Propionyl-CoA: 109.6
CHC-CoA: 108.5
Malonyl-CoA: 98.9
Isobutyryl-CoA: 85.3
Benzoyl-CoA: 72.7
3-Methylbutyryl-CoA: 52.4
Acetyl-CoA: 42.7
inactive: 38.4
fatty_acid: 29.4

PKS_AT (2103..2400): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 219.0
inactive: 113.8
Methoxymalonyl-CoA: 65.6
Methylmalonyl-CoA: 60.8
Propionyl-CoA: 37.5
Isobutyryl-CoA: 35.7
Ethylmalonyl-CoA: 23.7
2-Methylbutyryl-CoA: 21.8
Acetyl-CoA: 18.3
Benzoyl-CoA: 17.3
fatty_acid: 14.2
CHC-CoA: 11.6
trans-1,2-CPDA: 10.3
3-Methylbutyryl-CoA: 9.6

slnA9: pk

PKS_AT (566..866): pk

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Methylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 183.1
Ethylmalonyl-CoA: 175.4
Methoxymalonyl-CoA: 148.3
2-Methylbutyryl-CoA: 125.1
trans-1,2-CPDA: 93.3
Propionyl-CoA: 90.1
CHC-CoA: 88.4
Malonyl-CoA: 73.5
Isobutyryl-CoA: 67.6
fatty_acid: 38.3
3-Methylbutyryl-CoA: 37.4
Acetyl-CoA: 32.7
Benzoyl-CoA: 32.4
inactive: 14.8

AEZ53969.1: fatty_acid

CAL_domain (29..468): fatty_acid

minowa: fatty_acid

Prediction, score:

fatty_acid: 22.6
AHBA: 17.3
NH2: 9.4
shikimic_acid: 8.3
Acetyl-CoA: 0.2

Link to PARAS substrate predictor