BGC0000148 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000148 (Saccharopolyspora spinosa)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	80,161	A83543A/Spinosyn D	PKS:Type I	100%

BGC0000148 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 80,161 nt. (total: 80,161 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 80,162
Label		BGC0000148

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000148	1.00	Polyketide	A83543A	Saccharopolyspora spinosa
BGC0000041	0.36	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000166	0.31	Polyketide	tylactone	Streptomyces fradiae
BGC0001164	0.31	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001275	0.31	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276	0.30	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273	0.30	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001051	0.30	NRP, Polyketide	thuggacin A	Sorangium cellulosum
BGC0001163	0.29	Polyketide	1-heptadecene	Moorea producens 3L
BGC0000996	0.29	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000148	1.00	Polyketide	A83543A	Saccharopolyspora spinosa
BGC0000041	0.69	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000166	0.65	Polyketide	tylactone	Streptomyces fradiae
BGC0001164	0.65	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001275	0.64	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276	0.64	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273	0.64	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001051	0.63	NRP, Polyketide	thuggacin A	Sorangium cellulosum
BGC0001163	0.63	Polyketide	1-heptadecene	Moorea producens 3L
BGC0000996	0.63	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 1110 - 80161:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - ohmal) + (redmal) + (Me-ohmal - ohmal) + (ccmal - ohmal - ohmal) + (Me-ccmal - ccmal - ccmal)
Non-colinear ordering:: spnA + spnB + spnC + spnD + spnE

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

spnA

PKS_AT (528..824): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 83.3%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 216.7
Ethylmalonyl-CoA: 203.2
Methoxymalonyl-CoA: 178.8
trans-1,2-CPDA: 129.1
2-Methylbutyryl-CoA: 128.9
Malonyl-CoA: 123.9
CHC-CoA: 113.7
Propionyl-CoA: 104.4
Isobutyryl-CoA: 100.5
Benzoyl-CoA: 80.4
Acetyl-CoA: 70.6
fatty_acid: 30.5
inactive: 30.2
3-Methylbutyryl-CoA: 25.4

PKS_AT (1525..1826): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 181.4
inactive: 86.7
Methoxymalonyl-CoA: 71.7
Methylmalonyl-CoA: 57.2
Propionyl-CoA: 47.1
fatty_acid: 21.9
Acetyl-CoA: 20.4
Isobutyryl-CoA: 15.1
CHC-CoA: 14.7
Benzoyl-CoA: 13.0
trans-1,2-CPDA: 10.8
2-Methylbutyryl-CoA: 9.4
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -1.3

spnB

PKS_AT (572..873): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 181.4
inactive: 86.7
Methoxymalonyl-CoA: 71.7
Methylmalonyl-CoA: 57.2
Propionyl-CoA: 47.1
fatty_acid: 21.9
Acetyl-CoA: 20.4
Isobutyryl-CoA: 15.1
CHC-CoA: 14.7
Benzoyl-CoA: 13.0
trans-1,2-CPDA: 10.8
2-Methylbutyryl-CoA: 9.4
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -1.3

spnC

PKS_AT (564..861): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.7
Ethylmalonyl-CoA: 201.2
Methoxymalonyl-CoA: 174.0
2-Methylbutyryl-CoA: 128.5
trans-1,2-CPDA: 124.7
CHC-CoA: 100.2
Malonyl-CoA: 98.0
Propionyl-CoA: 92.7
Isobutyryl-CoA: 81.2
Benzoyl-CoA: 77.3
Acetyl-CoA: 43.9
3-Methylbutyryl-CoA: 40.2
inactive: 30.1
fatty_acid: 21.8

PKS_AT (2099..2398): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 66.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 191.0
inactive: 104.0
Methoxymalonyl-CoA: 73.8
Methylmalonyl-CoA: 56.4
Propionyl-CoA: 38.3
Isobutyryl-CoA: 33.1
fatty_acid: 21.7
trans-1,2-CPDA: 20.9
Acetyl-CoA: 19.4
Benzoyl-CoA: 17.3
2-Methylbutyryl-CoA: 12.7
CHC-CoA: 12.3
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.6

spnD

PKS_AT (576..872): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 193.2
inactive: 87.8
Methoxymalonyl-CoA: 77.5
Methylmalonyl-CoA: 61.6
Propionyl-CoA: 39.2
Isobutyryl-CoA: 23.9
fatty_acid: 23.4
Acetyl-CoA: 23.2
trans-1,2-CPDA: 15.7
CHC-CoA: 15.0
2-Methylbutyryl-CoA: 12.4
Benzoyl-CoA: 11.9
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -1.9

PKS_AT (2320..2619): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 66.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 192.4
inactive: 86.4
Methoxymalonyl-CoA: 72.5
Methylmalonyl-CoA: 59.0
Propionyl-CoA: 44.8
Isobutyryl-CoA: 31.6
Ethylmalonyl-CoA: 25.3
fatty_acid: 23.0
trans-1,2-CPDA: 20.8
Acetyl-CoA: 18.3
CHC-CoA: 18.2
2-Methylbutyryl-CoA: 12.8
Benzoyl-CoA: 12.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (3862..4160): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 173.5
Methoxymalonyl-CoA: 75.7
inactive: 65.4
Methylmalonyl-CoA: 55.7
Propionyl-CoA: 36.1
fatty_acid: 29.6
Isobutyryl-CoA: 29.6
Acetyl-CoA: 26.6
CHC-CoA: 21.9
2-Methylbutyryl-CoA: 18.3
Ethylmalonyl-CoA: 16.6
trans-1,2-CPDA: 15.0
Benzoyl-CoA: 14.1
3-Methylbutyryl-CoA: 8.3

spnE

PKS_AT (563..859): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 202.9
Ethylmalonyl-CoA: 194.9
Methoxymalonyl-CoA: 163.1
2-Methylbutyryl-CoA: 130.5
trans-1,2-CPDA: 123.5
Malonyl-CoA: 106.8
CHC-CoA: 103.1
Isobutyryl-CoA: 100.5
Propionyl-CoA: 98.0
Benzoyl-CoA: 65.4
Acetyl-CoA: 42.7
3-Methylbutyryl-CoA: 41.7
fatty_acid: 31.2
inactive: 29.0

PKS_AT (2320..2617): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 184.6
inactive: 88.8
Methoxymalonyl-CoA: 69.7
Methylmalonyl-CoA: 54.6
Propionyl-CoA: 36.4
fatty_acid: 25.4
Acetyl-CoA: 24.7
Isobutyryl-CoA: 23.9
trans-1,2-CPDA: 16.6
CHC-CoA: 13.9
2-Methylbutyryl-CoA: 8.5
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -1.5
Benzoyl-CoA: -2.3

PKS_AT (4093..4390): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 188.7
inactive: 84.6
Methoxymalonyl-CoA: 72.9
Methylmalonyl-CoA: 54.1
Propionyl-CoA: 36.7
trans-1,2-CPDA: 24.1
fatty_acid: 22.0
Acetyl-CoA: 18.9
Benzoyl-CoA: 16.9
Ethylmalonyl-CoA: 16.3
CHC-CoA: 11.5
2-Methylbutyryl-CoA: 10.8
Isobutyryl-CoA: 10.6
3-Methylbutyryl-CoA: 0.0